SHED′s Response to Various Pulpotomy Materials: Cytotoxicity and Gene Expression Analysis

2021 ◽  
Vol 45 (6) ◽  
pp. 406-413
Author(s):  
Viral Maru ◽  
Omkar Shetty ◽  
Uma Dixit ◽  
Vishal G Warke ◽  
Mansi Khande ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
Mtt Assay ◽  
Calcium Silicate ◽  
Live Cells ◽  
Alizarin Red ◽  
Cytotoxic Effects ◽  
Scratch Assay ◽  
Matrix Deposition ◽  
Post Test

Purpose : To examine the cytotoxicity and genetic expression of SHEDs cultured in eluates of various calcium silicate based pulpotomy materials. Study design : MTT assay, flow cytometry, alizarin red staining and scratch assay was used to assess the cellular viability, apoptosis, calcium matrix deposits and cell migration respectively. The gene expression of ALP, OCN and BMP -2, were measured with rtPCR. One way ANNOVA and Bonferroni post test was used for statistical analysis. Results : MTT assay analysis reported that all the test specimen had no cytotoxic effects. The highest number of live cells [ % ] was found in RetroMTA. The highest percentage of cell migration was observed in SHEDs cultured in EndoCem Zr. The mean absorbance for calcium matrix deposition was higher or similar in all test specimens, when compared to control groups. The expression of BMP -2 and OCN were significantly higher in cells exposed to RetroMTA and NeoMTA respectively after 24 hrs of incubation. After 72 hrs of incubation the mRNA expression of ALP was significantly higher in MTA. Conclusions: SHEDs cultured in eluates of various calcium silicate based cements exhibited cytocompatibility and maintained odontogenic like phenotype differentiation in SHEDs.

scholarly journals Comparison of Cytotoxic Effects of Calcium Silicate-based Materials on Human Pulp Fibroblasts

2019 ◽  
Vol 13 (4) ◽  
pp. 241-246 ◽  
Author(s):  
Mehmet Adıgüzel ◽  
Fuat Ahmetoğlu ◽  
Ayçe Ünverdi Eldeniz ◽  
Mehmet Gökhan Tekin ◽  
Bülent Göğebakan
Keyword(s):  
Flow Cytometry ◽  
Cell Viability ◽  
Mtt Assay ◽  
Calcium Silicate ◽  
Culture Medium ◽  
In Vitro Cytotoxicity ◽  
The Other ◽  
Cytotoxic Effects ◽  
Pulp Capping

Background. This study aimed to compare the in vitro cytotoxicity of Theracal LC, BiodentineTM, iRoot BP Plus, and MTA Angelus on human pulp fibroblasts (HPF). Methods. Fifteen discs from each calcium silicate-based material were prepared in sterile Teflon molds. After setting, the fabricated discs were eluated with a culture medium for 24 h. HPF cells were plated onto 24-well plates at 5×103 cells/well, and the cells were exposed to the material eluates. The cell viability was evaluated with MTT assay at three different times (24, 48, and 72 h). Data were statistically analyzed. The apoptotic/necrotic status of HPF cells exposed to material eluates was determined by flow cytometry. Results. The differences between the effects of Theracal LC, BiodentineTM, MTA Angelus, and iRoot BP Plus on HPF cells were found to be statistically significant (P<0.05). Theracal LC was found to be more cytotoxic considering other vital pulp capping materials at 24- (28.3%), 48- (44.9%), and 72-hour (49.2%) intervals. On the other hand, BiodentineTM showed the least cytotoxic effects (97.1%, 130.0%, and 103.7%, respectively) According to flow cytometry results, Theracal LC material increased apoptosis/necrosis ratios compared to the other materials. Conclusion. Based on the results of the present study, BiodentineTM, MTA Angelus, and iRoot BP Plus can be classified as biocompatible materials in vital endodontic treatments. However, the Theracal LC materials should be used carefully due to their cytotoxic effects.

scholarly journals Inhibition Capacity of the n-Hexane Fraction of Myrmecodia pendens as a Potential Anti-Cancer in Breast and Cervical Cancer: In Vitro Study

2020 ◽  
Vol 11 (3) ◽  
pp. 115
Author(s):  
Muhammad Hasan Bashari ◽  
Eveline Yuniarti ◽  
Tenny Putri ◽  
Nurul Qomarilla ◽  
Dikdik Kurnia ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cervical Cancer ◽  
Cell Death ◽  
Cell Migration ◽  
Mtt Assay ◽  
Hela Cells ◽  
Hexane Fraction ◽  
Scratch Assay ◽  
Anti Cancer ◽  
Mcf 7

Breast cancer (BC) and cervical cancer (CC) have a high prevalence and mortality rate worldwide. Despite the availability of advanced treatment, resistance to conventional chemotherapies has emerged. Myrmecodia pendens, one of the species of Sarang Semut (local name), possess a potential of antitumor effects by inducing cell death different cancer cell entities. This study aimed to assess anti-tumor activities of n-hexane fraction of M. pendens in inhibiting cell survival and cell migration in BC and CC cells. M. pendens was extracted in methanol then fractionated using n-hexane or ethyl acetate. BC cells including MCF-7 (luminal A), HCC-1954 (HER2+) cells and CC Hela cells were treated with M. pendens extracts to evaluate cytotoxic activity using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay as well as anti-cell migration using scratch assay. We also analyzed inhibitory concentration 50 (IC50) of n-hexane fraction in BC and CC cells. We started with comparing cytotoxicity activities of methanol extract, ethyl acetate and n-hexane fractions of M. pendens. Data showed that the n-hexane fraction was the most potent inducing BC cell death. Therefore, we used the n-hexane fraction for further experiments. Interestingly, IC50 of this fraction in HCC-1954 and Hela cells were lower than in MCF-7 cells, 16; 13 and 60 ppm, respectively. Moreover, the low concentrations of n-hexane fraction inhibited HeLa cells migration, compared to control group (p<0.05). The n-hexane fraction of M. pendens shows promising anti-cancer agent, by inhibiting BC and CC cell survival as well as inhibiting CC cells migration.Keywords: breast cancer, cervical cancer, MTT assay, Sarang Semut, scratch assay

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

scholarly journals Co-ordinated Gene Expression in the Liver and Spleen during Schistosoma japonicum Infection Regulates Cell Migration

2010 ◽  
Vol 4 (5) ◽  
pp. e686 ◽  
Author(s):  
Melissa L. Burke ◽  
Donald P. McManus ◽  
Grant A. Ramm ◽  
Mary Duke ◽  
Yuesheng Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
Schistosoma Japonicum

Knockdown of Thrombomodulin Enhances HCC Cell Migration through Increase of ZEB1 and Decrease of E-cadherin Gene Expression

2010 ◽  
Vol 17 (12) ◽  
pp. 3379-3385 ◽  
Author(s):  
Ming-Te Huang ◽  
Po-Li Wei ◽  
Jun-Jen Liu ◽  
Der-Zen Liu ◽  
Huang Huey-Chun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
E Cadherin

scholarly journals Lymphotoxin-β receptor-NIK signaling induces alternative RELB/NF-κB2 activation to promote metastatic gene expression and cell migration in head and neck cancer

2018 ◽  
Vol 58 (3) ◽  
pp. 411-425 ◽  
Author(s):  
Rita Das ◽  
Jamie Coupar ◽  
Paul E. Clavijo ◽  
Anthony Saleh ◽  
Tsu-Fan Cheng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Head And Neck Cancer ◽  
Cell Migration ◽  
Head And Neck ◽  
Neck Cancer ◽  
Β Receptor

scholarly journals Regulation of human glioma cell migration, tumor growth, and stemness gene expression using a Lck targeted inhibitor

Oncogene ◽  
2018 ◽  
Vol 38 (10) ◽  
pp. 1734-1750 ◽  
Author(s):  
J. P. Zepecki ◽  
K. M. Snyder ◽  
M. M. Moreno ◽  
E. Fajardo ◽  
A. Fiser ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Migration ◽  
Tumor Growth ◽  
Glioma Cell ◽  
Human Glioma ◽  
Human Glioma Cell ◽  
Glioma Cell Migration

Biological Effects of p53 Mutation W248 and H175 on MCF-7 Cells

2013 ◽  
Vol 790 ◽  
pp. 550-554
Author(s):  
Xiang Yu Zhou ◽  
Ya Jun Liu ◽  
Dan Li
Keyword(s):  
Flow Cytometry ◽  
Cell Proliferation ◽  
Cell Migration ◽  
Tumor Cell ◽  
Mtt Assay ◽  
Biological Effects ◽  
Scratch Test ◽  
Control Group ◽  
Tumor Cell Migration ◽  
Mcf 7

Objective: p53, a tumor suppressor gene, is one of the hotspots in the world of the biomedical field. Mutation of p53 gene, which is found in approximately 50% of human cancers, is a key event in carcinogenesis. This project aims to investigate the new characteristics of two p53 mutants, p53-W248 and p53-H175, in MCF-7 cells, so as to provide the experimental basis for understanding the functional alternations of mutant p53. Methods: In this study, MCF-7 cells transfected with p53-H175 or p53-W248 plasmids were used as experimental group and the MCF-7 cells transfected wild type p53 plasmid were used as control group. Then the biological effects at the cellular level were investigated using 3-(4.5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, flow cytometry analysis and cell scratch test. Results: MTT assay showed that p53-W248 might promote cell proliferation in MCF-7 cells. The results of flow cytometry indicated that no significant effect on cell cycle progression and cell apoptosis by p53-H175 or p53-W248 in cells. The cell scratch test showed that p53-H175 could increase the ability of cell migration. Conclusion: p53-H175 could lead to the promotion of tumor cell migration, while p53-W248 may promote tumor cell proliferation. p53-H175 and p53-W248 might have acquired some new characteristics of oncogenes.

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