Association of Amelogenin with High Caries Experience in Indian Children

2015 ◽  
Vol 39 (5) ◽  
pp. 458-461 ◽  
Author(s):  
R Saha ◽  
PB Sood ◽  
M Sandhu ◽  
A Diwaker ◽  
S Upadhyaye
Keyword(s):  
Dental Caries ◽  
Pcr Amplification ◽  
World Health ◽  
Caries Experience ◽  
Sequencing Data ◽  
Early Screening ◽  
Indian Children ◽  
Amelogenin Gene ◽  
Dental Caries Susceptibility ◽  
Health Organization

Objective: The purpose of this study was to identify alterations in amelogenin gene that are associated with dental caries susceptibility and to develop a non-invasive early screening test for caries risk. Study design: 60 individuals were selected for the study based on the inclusion and exclusion criteria and were divided into two groups based on DMFT score. DMFT was scored according to World Health Organization guidelines. Saliva obtained from all participants was stored in Indogenix DNA Self-Collection kits at 4°C. DNA was extracted according to the manufacturer's instructions. Once the entire DNA was isolated from each sample it was put forward for PCR amplification. The amplified amelogenin gene was then run on 2% agarose gel stained with ethidium bromide. The amplified gene was processed by SSCP technique to find out the altered bands and then subjected to DNA sequencing for identification of alterations in the amino acid sequence of amelogenin gene. Results: The sequencing data showed the presence of mutation. Samples showing mutation (43.3%) showed high correlation with caries (80.7%) experience which was statistically significant. Conclusion: Understanding the genetics of dental caries susceptibility will provide new insights into the caries process in individuals and will facilitate the development of targeted preventive strategies.

scholarly journals Prevalence of caries and DMF-T index with D1-D2 scale on students

2013 ◽  
Vol 25 (2) ◽  
Author(s):  
Setunggal Agung Nugroho Harahap ◽  
Sri Susilawati ◽  
Dede Sutardjo
Keyword(s):  
College Students ◽  
Dental Caries ◽  
Health Survey ◽  
Boarding School ◽  
Simple Random Sampling ◽  
World Health ◽  
Caries Experience ◽  
Health Habits ◽  
Factors Associated ◽  
Health Organization

Introduction: Many factors associated with poor health habits are initiated during adolescence. This is because as a teenager they have the feeling that they are unique and invulnerable that makes them think that the disease or disorder will not enter into their lives. Based on this, teenagers can have a bad habit for their oral health, thus increasing the level of caries in their mouths. The purpose of this study was to get data on the caries experience (DMF-T index) with D1-D2 scale on students. Method: This type of research is descriptive. The technique used in this sampling is simple random sampling, the sample is 266 students consisting of 109 men and 157 women. Prevalence rate of caries adjusted with 2004 Household Health Survey and DMFT category based on World Health Organization. Result: The results showed that the prevalence of caries in college students of Al-Ihsan Islamic boarding school is 90.6%. DMF-T index in college students of Al-Ihsan Islamic boarding school is 4.18. D1 scale that was found was 280 cases. D2 is the scale found 335 cases. and dental caries were found at 949 cases. Conclusion: Prevalence of caries and DMF-T index with D1-D2 scale on students are prevalence of dental caries is higher than prevalence in 2004 Household Health Survey. DMF-T index is  moderate, while the D1 scale found 280 cases and the D2 scale found 335 cases.

scholarly journals Dental caries experience in Indians of the Upper Xingu, Brazil

2001 ◽  
Vol 43 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Deborah Denise Leal RIGONATTO ◽  
José Leopoldo Ferreira ANTUNES ◽  
Paulo FRAZÃO
Keyword(s):  
Dental Caries ◽  
Age Groups ◽  
High Ratio ◽  
World Health ◽  
Caries Experience ◽  
Health Examination ◽  
Cultural Patterns ◽  
Care Services ◽  
Health Organization ◽  
Care Index

The present study describes the experience of dental caries in Indians communities of the Xingu, in order to supply parameters for further analysis of trends of the disease in Indians. We performed oral health examination in 288 Indians from four communities (Yawalapiti, Aweti, Mehinaku and Kamaiura) living in the southern part of the Xingu National Park, using international criteria defined by the World Health Organization. The outcome measures were the DMFT and dmft scores, and the care index. Indians of the Upper Xingu presented high levels of caries, in all age groups. The average DMFT for 11 to 13-year-old children - 5.93 - was lower than the index measured in 1993 for 12-year-old schoolchildren in nearby cities - 8.23 -, whose United Nations' human development index ranked medium. However, Indians presented a much lower care index, per age group, than these cities, and a high ratio of missing teeth for persons above 20 years old. These observations indicate low incorporation of dental care services. The irregularity of the services programmed for these communities, and the changing dietary and cultural patterns, mainly derived from their contact with the non-indigenous population of Brazil, reinforce the pressing need for health promotion initiatives aimed at these groups.

scholarly journals Dental caries in 6-12-year-old indigenous and non-indigenous schoolchildren in the Amazon basin of Ecuador

2008 ◽  
Vol 19 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Widman Medina ◽  
Anna-Karin Hurtig ◽  
Miguel San Sebastián ◽  
Edy Quizhpe ◽  
Cristian Romero
Keyword(s):  
Amazon Basin ◽  
Ethnic Origin ◽  
World Health ◽  
Caries Experience ◽  
Cross Sectional ◽  
Health Response ◽  
Indigenous Children ◽  
Prevention And Health Promotion ◽  
The Mean ◽  
Health Organization

The purpose of this study was to evaluate the caries experience among 6-12-year-old indigenous (Naporunas) and non-indigenous (recent settlers of mixed ethnic origin) schoolchildren, living in the Amazon basin of Ecuador. Cross-sectional data were obtained from 1,449 clinical exams according to the World Health Organization criteria. Nine (7.6%) indigenous and 3 (4.5%) non-indigenous children had no caries experience in their primary dentition at the age of 6. The mean dmft value (SD) among indigenous and non-indigenous children aged 6 was 6.40 (3.36) and 8.36 (3.93), respectively. Sixty-four (54.2%) indigenous and 29 (43.3%) non-indigenous children had no caries experience in their permanent first molars at the age of 6. Only 7 (6.26%) indigenous and 2 (2.60%) non-indigenous children were caries-free at the age of 12. The mean DMFT values (SD) for 12-year-olds were 4.47 (2.85) among indigenous and 5.25 (2.89) among non-indigenous children. Fillings were almost non existent. Caries rates were high among both groups, with untreated carious lesions predominating in all ages. The data of indigenous children suggest adoption of a non-traditional diet. An appropriate oral health response based primarily on prevention and health promotion is needed.

scholarly journals Avaliação das Variantes Genéticas dos Genes AMELX e ENAM na Cárie Dentária em Adolescentes

2021 ◽  
Vol 24 (5-esp.) ◽  
pp. 650-654
Author(s):  
Gabriela Paschoalini Romagni ◽  
Paula Marino Costa ◽  
Sandra Mara Maciel ◽  
Maria Paula Jacobucci ◽  
Regina Célia Poli-Frederico
Keyword(s):  
Genetic Component ◽  
World Health ◽  
Caries Experience ◽  
Nucleotide Polymorphisms ◽  
Chi Square ◽  
Single Nucleotide ◽  
Significance Level ◽  
Exact Test ◽  
Polymerase Chain ◽  
Health Organization

A doença cárie é considerada, atualmente, como biofilme sacarose dependente, entretanto, estudos recentes apontam que fatores genéticos também podem influenciar seu desenvolvimento. Variantes nos gene amelogenina (AMELX) e enamelina (ENAM), responsáveis pela formação do esmalte, têm sido propostas como potencialmente envolvidos na doença. O objetivo deste estudo foi avaliar se a ocorrência de cárie dentária em adolescentes está relacionado às variantes nos genes AMELX e ENAM. Para a avaliação da prevalência de cárie foi utilizado o índice de dentes cariados, perdidos e obturados (CPO-D), segundo critérios da Organização Mundial de Saúde. As amostras de DNA foram extraídas das células da mucosa oral. Para a análise dos polimorfismos de nucleotídeo único (SNPs) dos genes AMELX (rs17878486) e ENAM (rs7671281) foi utilizada  a técnica de amplificação de fragmentos de DNA pela reação em cadeia da polimerase foi realizada (PCR) em tempo real pelo sistema TaqMan (Applied Biosystems, Foster City, EUA). Para a análise estatística, foi utilizado o teste exato de Fisher e qui-quadrado com nível de significância de 5%. Apenas os fatores socioeconômicos influenciaram a experiência de cárie. Concluiu-se que o componente genético, na população deste estudo, não influenciou o desenvolvimento da cárie.   Palavras-chave: Polimorfismo genético. Adolescentes. Esmalte.   Abstract Caries disease is currently considered a sucrose-dependent biofilm, however recent studies indicate that a genetic component can also influence its development. Variants in the amelogenin (AMELX) and enamelin (ENAM) genes, responsible for the enamel formation, have been proposed as potentially involved in the disease. The purpose of this study was to evaluate whether the occurrence of dental caries in adolescents is related to variants in the AMELX and ENAM genes. To assess the caries prevalence, the index of decayed, missing and filled teeth (DMFT) were used, according to World Health Organization criteria. DNA samples were extracted from oral mucosa cells. For the analysis of single nucleotide polymorphisms (SNPs) of the AMELX (rs17878486) and ENAM (rs7671281) genes, the amplifying DNA fragments technique  by the polymerase chain reaction was performed (PCR) in real time by the TaqMan system (Applied Biosystems, Foster City, USA). For the statistical analysis, Fisher's exact test and chi-square were used with a 5% significance level. Only socioeconomic factors influenced the caries experience. It was concluded that the genetic component in the population of this study, did not influence the development of caries.   Keywords: Genetic polymorphism. Adolescents. Enamel.

Probiotics in Dental Caries Prevention

2019 ◽  
Vol 56 (1) ◽  
pp. 84 ◽  
Author(s):  
Padmapriya Ramanujam ◽  
Saravanan Poorni ◽  
Manali Ramakrishnan Srinivasan ◽  
Nivedhitha Malli Sureshbabu
Keyword(s):  
Dental Caries ◽  
Dental Health ◽  
Experimental Studies ◽  
Health Benefit ◽  
Potential Method ◽  
Caries Prevention ◽  
World Health ◽  
Oral Diseases ◽  
Chewing Gums ◽  
Health Organization

The prevalence of oral diseases is increasing and is becoming a major concern in the society. Treatment without prevention is simply unsustainable. One such preventable disease is dental caries. Several strategies for caries prevention have been developed over the years. Bacteriotherapy is one potential method. With better understanding on the importance of a well balanced oral microbial environment for maintaining good dental health, probiotics has gained great momentum in dentistry. The World Health Organization along with the Food and Agriculture Organisation of the United Nations defined probiotics as “Live microorganisms which when administered in adequate amounts confer a health benefit on the host”. Several probiotic strains have been identified for caries prevention through various experimental studies. However, identifying the right vehicle for administration of probiotics on everyday basis is the need of the hour. Toothpastes, mouthrinses, powders, tablets, lozenges, chewing gums are some probiotic vehicles that have been studied. This paper would throw light on the microbial strains, mechanism of action, forms and future for probiotics in caries prevention.

scholarly journals Population-Based Estimation of Dental Caries and Periodontal Disease Rates of Gravid and Recently Postpartum Women in Lilongwe, Malawi

2019 ◽  
Vol 09 (03) ◽  
pp. e268-e274
Author(s):  
Kathleen M. Antony ◽  
Peter N. Kazembe ◽  
Ryan M. Pace ◽  
Judy Levison ◽  
Jesse Mlotha-Namarika ◽  
...  
Keyword(s):  
Dental Caries ◽  
Periodontal Disease ◽  
Health Workers ◽  
Population Based ◽  
Oral Health Status ◽  
World Health ◽  
Postpartum Women ◽  
Dental Disease ◽  
Dmf Index ◽  
Health Organization

Objectives The objective of this study was to determine the rate of dental caries and periodontal disease among gravid and recently postpartum women at five delivery centers within and surrounding Lilongwe, Malawi. Study Design We partnered with obstetric specialists, community health workers, and dentists to perform dental history interviews and dental examinations during the study period from December 2012 to May 2014. Dental examinations were performed according to World Health Organization standards to assess periodontal and oral health status. Results Among the 387 gravid and recently postpartum women, the rate of dental caries was 69.3% and the rate of composite dental disease (caries and periodontal disease) was 76.7%. The majority (69.5%) of women examined had a decayed-missing-filled (DMF) index greater than or equal to one; the average DMF Index was 2.48. The majority of women had never seen a dentist (62.8%). However, most did perform oral hygiene, two or more times per day (90.2%); most women reported brushing with toothpaste (88.1%). Conclusion When assessing this population for dental caries and periodontal disease, the rate of dental disease was high. Therefore, this may be an ideal setting to test for impactful interventions aimed at reducing caries and periodontal disease.

scholarly journals Molecular profiling predicts meningioma recurrence and reveals loss of DREAM complex repression in aggressive tumors

2019 ◽  
Vol 116 (43) ◽  
pp. 21715-21726 ◽  
Author(s):  
Akash J. Patel ◽  
Ying-Wooi Wan ◽  
Rami Al-Ouran ◽  
Jean-Pierre Revelli ◽  
Maria F. Cardenas ◽  
...  
Keyword(s):  
World Health ◽  
Sequencing Data ◽  
Histopathological Classification ◽  
Considerable Uncertainty ◽  
Cell Cycle Activation ◽  
Targeted Treatments ◽  
Meningioma Recurrence ◽  
Aggressive Tumors ◽  
Biochemical Analyses ◽  
Health Organization

Meningiomas account for one-third of all primary brain tumors. Although typically benign, about 20% of meningiomas are aggressive, and despite the rigor of the current histopathological classification system there remains considerable uncertainty in predicting tumor behavior. Here, we analyzed 160 tumors from all 3 World Health Organization (WHO) grades (I through III) using clinical, gene expression, and sequencing data. Unsupervised clustering analysis identified 3 molecular types (A, B, and C) that reliably predicted recurrence. These groups did not directly correlate with the WHO grading system, which classifies more than half of the tumors in the most aggressive molecular type as benign. Transcriptional and biochemical analyses revealed that aggressive meningiomas involve loss of the repressor function of the DREAM complex, which results in cell-cycle activation; only tumors in this category tend to recur after full resection. These findings should improve our ability to predict recurrence and develop targeted treatments for these clinically challenging tumors.

scholarly journals Persistent Detection and Infectious Potential of SARS-CoV-2 Virus in Clinical Specimens from COVID-19 Patients

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1384
Author(s):  
Michael Zapor
Keyword(s):  
Pcr Amplification ◽  
World Health ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Distinctive Features ◽  
Clinical Specimens ◽  
Polymerase Chain ◽  
Health Organization ◽  
Infectious Potential ◽  
Infective Potential

The Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) that emerged in December 2019 as the causative agent of Coronavirus 2019 (COVID-19) and was declared a pandemic by the World Health Organization in March 2020 has several distinctive features, including extensive multiorgan involvement with a robust systemic inflammatory response, significant associated morbidity and mortality, and prolonged persistence of viral RNA in the clinical specimens of infected individuals as detected by Reverse Transcription Polymerase Chain Reaction (RT-PCR) amplification. This review begins with an overview of SARS-CoV-2 morphology and replication and summarizes what is known to date about the detection of the virus in nasal, oropharyngeal, and fecal specimens of patients who have recovered from COVID-19, with a focus on the factors thought to contribute to prolonged detection. This review also provides a discussion on the infective potential of this material from asymptomatic, pre-symptomatic, and convalescing individuals, to include a discussion of the relative persistence and infectious potential of virus in clinical specimens recovered from pediatric COVID-19 patients.

scholarly journals Correlating Dental Caries with Tooth Brushing Frequency, Type of Toothpaste and Dental Visits

2014 ◽  
Vol 02 (01) ◽  
pp. 009-015
Author(s):  
Vaibhav Munjal ◽  
Ritu Jindal ◽  
Rohini Dua ◽  
Ripin Garewal ◽  
Parveen Bathla
Keyword(s):  
Dental Caries ◽  
Age Groups ◽  
Cross Sectional Study ◽  
Tooth Brushing ◽  
World Health ◽  
Dental Clinic ◽  
Cross Sectional ◽  
Dental Visits ◽  
Dental Professional ◽  
Health Organization

Abstract Aim: This study was done to evaluate correlation of Dental Caries status with frequency of tooth brushing, type of toothpaste and regular dental visits in children. Materials and methods: This cross-sectional study was carried out randomly on 2500 school children of two age groups viz. 12 and 15 years, and caries examination was done using WHO (World Health organization) 1997 criteria in order to correlate with various risk factors viz. brushing frequency, use of fluoridated/non-fluoridated toothpaste and frequency of child's visit to dental clinic. Results: In both the age groups brushing frequency and type of toothpaste used insignificantly affected caries while evidence that frequency of child's visit to dental clinic reduces caries risk was also not substantial. Conclusion: The present study implicates that due to complex multifactorial nature of dental caries, its prevention still remains an enigma for the dental professional and society.

scholarly journals The prevalence of dental erosion in 5-year-old preschoolers in Sharjah, United Arab Emirates

2016 ◽  
Vol 10 (02) ◽  
pp. 215-219 ◽  
Author(s):  
Vellore Kannan Gopinath
Keyword(s):  
United Arab Emirates ◽  
Dental Health ◽  
Dental Erosion ◽  
World Health ◽  
Caries Experience ◽  
Tooth Erosion ◽  
The World ◽  
Erosion Index ◽  
Health Organization ◽  
The Uk

ABSTRACT Objective: The aim of this study was to determine the percentage of 5-year-old preschoolers in Sharjah, affected by dental erosion and to assess the predictors. Materials and Methods: A total of 403 5-year-old children were examined of which 48.14% (n = 194) were boys and 51.86% (n = 209) were girls; 31.27% (n = 126) were Emirati and 68.73% (n = 277) were non-Emirati Arabs. Examination of dental erosion was confined to palatal surfaces of maxillary incisors using the erosion index described in the UK National Survey of Children's Dental Health, 1993. Dental caries was charted using the World Health Organization 1997 criteria. Results: In the sample of 403 5-year-old preschoolers examined, dental erosion was apparent in 237 (58.80%) children, with 55.09% showing the dissolution of enamel and 3.72% exhibiting exposed dentin. Predictors of dental erosion as determined by logistic regression concluded that compared to Emirati citizens other Arab nationalities have 0.27 times the odds (95% confidence interval [CI] =0.18–0.42) of having tooth erosion (P < 0.05). Children with caries experience have 0.28 times the odds (95% CI = 0.16–0.51) of having tooth erosion compared to children with no caries experience (P < 0.05). Children who drink sugary or carbonated beverages have 0.30 times the odds (95% CI = 0.19–0.41) of having dental erosion compared to children who drink water (P < 0.05). Conclusions: The findings of this study indicate that 58.80% of 5-year-old preschoolers in Sharjah, United Arab Emirates, were affected by dental erosion. Caries experience and consumption of acidic drinks were associated with dental erosion.

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