scholarly journals Development of the method for spectrophotometric quantitative determination of the total flavonoids in Bupleurum multinerve herb

2020 ◽  
Vol 20 (5-6) ◽  
pp. 170-174
Author(s):  
Svetlana A. Petukhova ◽  
Alina A. Posokhina ◽  
Vera M. Mirovich
Keyword(s):  
Quantitative Determination ◽  
Plant Material ◽  
Raw Materials ◽  
Complexing Agent ◽  
Optimal Conditions ◽  
Differential Spectrophotometry ◽  
Analytical Wavelength ◽  
The Mean ◽  
Analytical Range

The article presents the method for the quantitative determination of flavonoids for the analysis of Bupleurum multinerve herb based on the method of differential spectrophotometry. The optimal conditions for analysis have been determined. They include extractant 40% ethyl alcohol, ratio of raw materials and extractant 1 : 100, extraction time of 60 minutes in the boiling water bath, complexing agent of 1 ml of 2% aluminum chloride. The use of rutin as a standard has been experimentally validated, analytical wavelength is 412 nm. The relative error of the mean result (for n = 9) was 3.20%. Validation studies of the method have shown that it meets the criteria: linearity (r = 0.99988), correctness, specificity, and precision. The analytical range of the method is 8.6726.08 g/ml. The method is recommended for the inclusion into the new edition of the Pharmacopoeia Monograph for this type of plant material.

scholarly journals QUANTITATIVE DETERMINATION OF THE AMOUNT OF CAROTENOIDS IN THE FRUITS OF CHINESE LYCIUM CHINENSE MILL.

2020 ◽  
pp. 139-144
Author(s):  
Yevgeniy Yevgen'yevich Kurdyukov ◽  
Yelena Fedorovna Semenova ◽  
Inessa Yakovlevna Moiseyeva ◽  
Natal'ya Aleksandrovna Gavrilova ◽  
Tat'yana Andreyevna Ponomareva
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
Raw Material ◽  
Lycium Chinense ◽  
Optimal Conditions ◽  
Single Determination ◽  
Layer Chromatography ◽  
Β Carotene ◽  
Acetone Hexane

The objects of the study were Mature dried fruits of Chinese birch (Lycium chinense Mill., this. Solanaceae (Solanaceae)). Four samples were examined: No. 1 (Gifts of Pamir, Russia), No. 2 (Gullin Tianhe Pharmaceutical, China), No. 3 (A. J. Alliance, Russia), No. 4 (Globaltorg, Russia). The purpose of this work is to determine the amount of carotenoids in the fruits of Chinese by spectrophotometry. The quantitative determination of the amount of carotenoids in the raw material of Chinese Dereza was carried out. Thin-layer chromatography was used to confirm the presence of carotenoids in the fruits of Dereza. The expediency of using the spectrophotometric method for the detection and quantitative determination of carotenoids in extracts from the fruits of Chinese birch is substantiated. The electron spectra of hexane, acetone and acetone-hexane solutions in the wavelength range 400–500 nm have a maximum optical density at 450±2 nm, characteristic of β-carotene. The optimal conditions of extraction of carotenoids from the raw materials of this plant (extractant – hexane; ratio "raw material–extractant" – 1 : 5; extraction time – 90 minutes; the degree of grinding of raw materials – 0.5 mm). It was determined that the error of a single determination of the content of carotenoids in the fruits of Chinese Dereza with a confidence probability of 95% is ±3,49%. It was revealed that the content of carotenoids in the raw material of Chinese Dereza varies in the range of 33–39 mg%.

scholarly journals METHOD OF QUANTITATIVE DETERMINATION OF THE SUM OF TANNINS IN EUTERPE OLERACEA FRUITS

2021 ◽  
pp. 225-229
Author(s):  
Evgeniy Evgen'yevich Kurdyukov ◽  
Ольга Александровна Vodop'yanova ◽  
Natalia Victorovna Antropova ◽  
Aleksandr Vladimirovich Mitishev ◽  
Natal'ya Evgen'yevna Evgrashkina
Keyword(s):  
Hydrochloric Acid ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Raw Material ◽  
Average Error ◽  
Euterpe Oleracea ◽  
Optimal Conditions ◽  
Ammonium Alum ◽  
Direct Spectrophotometry

Fruits of Euterpe oleracea are widely used in foreign medical practice as an antioxidant. The fruits of Euterpe contain tannins. The most common method of quantitative determination of tannins is spectrophotometry. The purpose of this work is to determine the content of the sum of tannins in the fruits of Euterpe by spectrophotometry Quantitative determination of the amount of tannins in the fruits of Euterpe by direct spectrophotometry was carried out. To confirm the presence of tannins in the fruits of Euterpe, qualitative reactions were used (1% solution of iron-ammonium alum, 1% solution of vanillin in concentrated hydrochloric acid). The presence of tannins was confirmed by direct spectrophotometry in extracts from euterpe fruits, the analytical maxima of the studied compounds were determined at about 282±2 nm, which corresponds to the maximum absorption of catechin. The optimal conditions for the extraction of tannins from the raw materials of this plant (extractant – ethyl alcohol 40%; the ratio of "raw material – extractant" – 1 : 100; extraction time – 60 minutes; the degree of grinding of raw materials – 1.0 mm) are justified. It was determined that the average error in determining the content of tannins in the fruits of euterpe with a confidence probability of 95% is ±1.59%. It was revealed that the content of tannins in the fruits of euterpe is 8.90%.

DEVELOPMENT OF A METHOD FOR QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN TRIPLEUROSPERMUM INODORUM’S FLOWERS

2021 ◽  
pp. 157-166
Author(s):  
Blinova O.L. ◽  
Gileva A.A. ◽  
Hlebnikov A.V. ◽  
Belonogova V.D. ◽  
Turyshev A.Y.
Keyword(s):  
Quantitative Determination ◽  
Aluminum Chloride ◽  
Chloride Solution ◽  
Uv Spectra ◽  
Raw Material ◽  
Spectral Studies ◽  
Scientific Medicine ◽  
Differential Spectrophotometry ◽  
The Stability

Chamomilla recutita is used in scientific medicine. Tripleurospermum inodorum (L.) Sch. Bip. is widely spread among possible impurities of Chamomilla recutita (L.) Rauschert. This plant is perspective for establishment into scientific medicine but it can’t change Chamomilla recutita (L.) Rauschert. Purpose of research: development of a method for the quantitative determination of the amount of flavonoids in terms of rutin in Tripleurospermum inodorum’s flowers using differential spectrophotometry. Materials and methods. The samples prepared in different parts of Russia were used as objects of research. (2017 – 2020). Spectral studies were carried out in the wavelength range of 350-430 nm with a step of 1 nm using a spectrophotometer SF-2000. Results. To determine the analytical wavelength, the UV spectra of alcohol extraction of Tripleurospermum inodorum’s flowers were studied. Maximum of absorption was noticed at wavelength 370 nm. The differential spectrum of the same extraction with an aluminum chloride solution of 2% in 96% alcohol has a maximum at a length of 410±2 nm, which coincides with the maximum of the Standard Sample (SS) of rutin. The largest number of flavonoids is extracted by 70% alcohol. The maximum optical density and the highest output of the number of flavonoids from the raw material is observed at a degree of grinding of 2 mm with a single extraction for 60 minutes. In the conditions of complexation, the optimal ratio of the volume of the test solution and aluminum chloride with a solution of 2% in 96% alcohol is the ratio of 1:1. The stability of the complex with an aluminum chloride solution of 2% in 96% alcohol is observed in 40 minutes after the start of the reaction and retains it for 30 minutes. Findings. The method is developed and the parameters of the quantitative determination of the amount of flavonoids in Tripleurospermum inodorum flores are determined in terms of rutin using differential spectrophotometry.

scholarly journals Solid-Phase Extraction and Gas Chromatography with Mass Spectrometric Determination of Capsaicin and Some of Its Analogues from Chili Peppers (Capsicum spp.)

1999 ◽  
Vol 82 (6) ◽  
pp. 1399-1405 ◽  
Author(s):  
Philemon Manirakiza ◽  
Adrian Covaci ◽  
Paul Schepens
Keyword(s):  
Gas Chromatography ◽  
Quantitative Determination ◽  
Solid Phase ◽  
Accurate Method ◽  
Mass Spectrometric ◽  
Phase Extraction ◽  
Capsicum Spp ◽  
The Mean ◽  
Chili Peppers

Abstract A rapid and accurate method has been developed for the quantitative determination of capsaicin and its most important analogues, dihydrocapsaicin and nordihydrocapsaicin in chili peppers. These components were extracted with methylene chlo ride and separated from interfering substances with activated charcoal. Further cleanup on Florisil cartridges and elution with ethyl acetate were performed before gas chromatographic with mass spectrometric quantitation. The concentrations found were 440 ± 64 μg/g capsaicin, 81 ± 10 μg/g dihydrocapsaicin, and 11 ± 2 μg/g nordihydrocapsaicin. The mean recovery values for triplicate analysis were between 85-94%.

A Radio-Gas Chromatographic Method for Determining the Specific Radioactivity of Glycolic Acid in 14C-Labeled Leaf Tissue

1975 ◽  
Vol 53 (5) ◽  
pp. 609-614 ◽  
Author(s):  
J. D. Mahon ◽  
K. Egle ◽  
H. Fock
Keyword(s):  
Quantitative Determination ◽  
Plant Material ◽  
Glycolic Acid ◽  
Chromatographic Method ◽  
Specific Radioactivity ◽  
Leaf Tissue ◽  
Acid Solutions ◽  
Complete Procedure ◽  
Gas Chromatographic Method

A method for the extraction and quantitative determination of both the mass and radioactivity of glycolic acid from 14C-labeled leaf tissue is described. The recoveries of both mass and radioactivity from standard [1-14C]glycolic acid solutions averaged 98%, and recovery of radioactivity added to plant samples as [1-14C]glycolic acid was over 90% after the complete procedure. The method was reliable with total samples containing as little as 130 nmol of glycolic acid.The mass of glycolic acid recovered from sunflower leaf tissue was proportional to the amount of tissue extracted. In experiments with different plant material, the amount of glycolic acid varied between 530 and 1120 nmol/dm2 of leaf tissue. The specific radioactivity of the glycolic acid in sunflower leaf tissue during photosynthesis in 14CO2 was never more than 20% of the specific radioactivity of the 14CO2 supplied.

EVALUATION OF FLAME PHOTOMETRIC DETERMINATION OF MAGNESIUM IN PLANT MATERIAL

1964 ◽  
Vol 44 (6) ◽  
pp. 520-524
Author(s):  
A. A. MacLean
Keyword(s):  
Standard Error ◽  
Plant Material ◽  
Photometric Determination ◽  
Flame Photometry ◽  
Mean Values ◽  
Edta Titration ◽  
The Mean ◽  
Flame Method ◽  
Oxyhydrogen Flame

Flame methods for the determination of magnesium in plant material were compared with EDTA titration and a thiazole yellow procedure. Mean values for quadruplicate determinations on 24 samples were 0.544, 0.543, 0.532, and 0.514% for EDTA, flame photometry with an oxyhydrogen flame, thiazole yellow, and flame photometry with an oxyacetylene flame respectively.The degree of precision, as indicated by the standard error of the mean, was highest for the oxyhydrogen flame method and lowest when the oxyacetylene flame was used.Silica repressed magnesium emission with either flame source but degree of interference was greater with the oxyhydrogen flame.

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