scholarly journals INFLUENCE OF SEMAX ON THE INTENSITY OF LIPID PEROXIDATION IN IMMUNOCOMPETENT ORGANS IN THE CONDITIONS OF “SOCIAL” STRESS

2019 ◽  
Vol 19 (1S) ◽  
pp. 188-191
Author(s):  
M A Samotrueva ◽  
A L Yasenyavskaya ◽  
V Kh Murtalieva ◽  
N F Myasoedov ◽  
L A Andreeva
Keyword(s):  
Lipid Peroxidation ◽  
Social Stress ◽  
Initial Level ◽  
Thiobarbituric Acid ◽  
Male Rats ◽  
Sensory Contact ◽  
Corrective Effect ◽  
Immunocompetent Organs ◽  
Thymus Tissue ◽  
Tissue Homogenates

This study is devoted to an experimental study of the effect of Semax (Met-Glu-His-Phe-Pro-Gly-Pro) on the intensity of lipid peroxidation of the thymus and spleen lipids of male rats under conditions of “social” stress. “Social” stress in animals was modeled by the formation of aggressive and submissive behavior of males in the conditions of distant sensory contact. The intensity of lipid peroxidation (LPO) in the thymus and spleen was determined by spectrophotometry using three indicators: the initial level of products reacting with thiobarbituric acid (TBA-reactive products), the rate of spontaneous and induced by ascorbate and iron ions of LPO. “Social” stress is accompanied by an increase in peroxidation processes in immunocompetent organs, which contributes to the development of stress-induced functional disorders of the immune system. Against the background of Semax administration under “social” stress, its pronounced corrective effect on lipid peroxidation rates is observed, as evidenced by a decrease in spleen and thymus tissue homogenates of male rats in the initial level of TBA-reactive products, as well as spontaneous and ascorbate-dependent levels of lipid peroxidation.

scholarly journals Organ-Specific Oxidative Events under Restrictive Versus Full Reperfusion Following Hemorrhagic Traumatic Shock in Rats

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2195
Author(s):  
Carina Penzenstadler ◽  
Anna Zifko ◽  
Mohammad Jafarmadar ◽  
Janin Schulte ◽  
Joachim Struck ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Thiobarbituric Acid ◽  
Traumatic Shock ◽  
Male Rats ◽  
Resonance Spectroscopy ◽  
Stress Reactions ◽  
Paramagnetic Resonance ◽  
Early Reperfusion ◽  
Ros Formation

Background aim: Reperfusion after hemorrhagic traumatic shock (HTS) is often associated with complications that are partly ascribed to the formation of reactive oxygen species (ROS). The aim of our study was to compare the effects of restrictive reperfusion (RR) to rapid full reperfusion (FR) on ROS formation and/or oxidative events. Materials and methods: Anesthetized male rats were randomly subjected to HTS followed by FR (75 mL/kg/h) or RR (30 mL/kg/h for 40 min, followed by 75 mL/kg/h) with Ringer’s solution (n = 8/group). Compartment-specific ROS formation was determined by infusion of ROS scavenger 1-hydroxy-3-carboxy-2,2,5,5-tetramethyl-pyrrolidine hydrochloride (CP-H) during resuscitation, followed by electron paramagnetic resonance spectroscopy. Sham-operated animals (n = 8) served as controls. The experiment was terminated 100 min post-shock. Results: Mean arterial pressure was significantly higher in the FR compared to the RR group during early reperfusion. Only RR animals, not FR animals, showed significantly higher ROS concentrations in erythrocytes (1951 ± 420 vs. 724 ± 75 AU) and in liver (474 ± 57 vs. 261 ± 21 AU) compared to sham controls. This was accompanied by elevated alanine aminotransferase and creatinine levels in RR animals compared to both shams and FR animals, while lipid peroxidation products (thiobarbituric acid reactive substances) were significantly increased only in the kidney in the FR group (p < 0.05). RR animals showed significantly higher plasma peroxiredoxin-4 values when compared to the FR group (20 ± 2 vs. 14 ± 0.5 RLU). Conclusion: Restrictive reperfusion after HTS is associated with increased ROS formation in erythrocytes and liver compared to sham controls. Moreover, the restrictive reperfusion is associated with a more pronounced injury to the liver and kidney, which is likely mediated by other than lipid peroxidation process and/or oxidative stress reactions.

scholarly journals Changes of Prooxidant-Antioxidant Systems in Experimental Acute Pancreatitis

2017 ◽  
Vol 24 (3) ◽  
Author(s):  
Viktoria Cherkasova ◽  
Luibomyr Zaiats
Keyword(s):  
Lipid Peroxidation ◽  
Acute Pancreatitis ◽  
White Male ◽  
Thiobarbituric Acid ◽  
Activity Level ◽  
Male Rats ◽  
Antioxidant Systems ◽  
Diene Conjugates ◽  
Pronounced Increase ◽  
Oxidation Rates

Mortality in acute destructive pancreatitis, despite the development and introduction of new methods of treatment, remains stable high and in severe forms reaches 25-85%. Activation of neutrophils and macrophages in acute pancreatitis leads to an "oxygen burst", which is closely linked with the activation of lipid peroxidation.Goals. The purpose is to establish dynamic changes in the indexes of prooxidant-antioxidant systems in acute L-arginine-induced pancreatitis.Materials and methods. The study was performed on 62 white male rats of Wistar line weighing 180-220g, with modeled acute pancreatitis. Blood for analysis have been taken: the blood serum on 12, 24, 48 and 72 hours of experiment to determine the activity level of thiobarbituric acid products, diene conjugates, catalase and lactate for assessment of the intensity of oxidative stress and antioxidant systems.Results. The obtained results of the study showed that acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation processes (LPO). Revealed that the most pronounced increase in all blood parameters is observed 24 hours after the beginning of the study. A significant increase in the active products of tiobarbituric acid (TBA-AP) and diene conjugates (DC) was detected - 1.98 and 2.7 times, respectively, and 2.2 times the growth of catalase (CT). At the next stage of the experiment there is a slowdown in the rate of LPO, as evidenced by the following values. Thus, for 48 years in the 3rd group: TBA-AP - they increased by 5.1% (p> 0.05), DC - by 3.3% (p> 0.05), and the level of CT - by 43.4% (P <0.05), compared with data for 24 hours. It is important to note that at 72 hours, the CT level decreased by 23.3% (p> 0.05), which may indicate an exhaustion of antioxidant systems. Indicators of LPO on 72 hours compared with 48 hours in group III: TBA-AP - increased by 1.7% (p> 0.05), DC - by 5.7% (p> 0.05).Conclusions. Acute L-arginine-induced pancreatitis is accompanied by an intensification of lipid peroxidation-oxidation processes that can potentiate the development of multiple organ failure in pancreatic inflammation. The most pronounced changes in lipid peroxidation-oxidation rates are observed for 24 hours of study.

scholarly journals Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats

2010 ◽  
Vol 3 (4) ◽  
pp. 109-117 ◽  
Author(s):  
Angelika Puzserova ◽  
Iveta Bernatova
Keyword(s):  
Nitric Oxide ◽  
Social Stress ◽  
Thiobarbituric Acid ◽  
Male Rats ◽  
No Production ◽  
Chronic Social Stress ◽  
Living Space ◽  
Dependent Component ◽  
Wistar Kyoto ◽  
Oxidative Load

Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive ratsThe aim of this study was to examine oxidative load and endothelium-dependent vasorelaxation in the serotonin pre-constricted femoral artery (FA) of Wistar-Kyoto (WKY) rats exposed to chronic social stress produced by crowding in the presence or absence of ascorbic acid (AsA) in working solution. Adult male rats were randomly divided into control (living space: 480 cm2/rat) or stressed (living space: 200 cm2/rat) groups for 8 weeks. Blood pressure and heart rate, determined using tail-cuff plethysmography, were not influenced by stress vs. control. Conjugated dienes (CD) and concentrations of thiobarbituric acid-reactive substances (TBARS) were measured in the left ventricle and liver (for assessment of oxidative load) and were found unchanged by chronic crowding. The nitric oxide (NO)-dependent component of endothelium-dependent relaxation was investigated in the FA using a wire myograph. In both the presence and absence of AsA, acetylcholine-induced relaxation of the FA of stressed rats significantly exceeded that of the controls, which was associated with an increase of the NO-dependent component. In conclusion, the data showed that chronic crowding did not produce oxidative stress in the organs investigated and indicate that elevation of NO production during chronic stress is an important way of adaptation, which may prevent normotensive rats from the development of stress-induced hypertension.

scholarly journals α-Tocopherol Protects the Heart, Muscles, and Testes from Lipid Peroxidation in Growing Male Rats Subjected to Physical Efforts

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Magdalena Górnicka ◽  
Anna Ciecierska ◽  
Jadwiga Hamulka ◽  
Małgorzata E. Drywień ◽  
Joanna Frackiewicz ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Experimental Period ◽  
Treadmill Running ◽  
Male Rats ◽  
Rat Tissues ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Tocopherol Concentration

The effect of α-tocopherol supplementation on adaptation to training is still equivocal. The aim of the study was to determine the effect of training and α-tocopherol supplementation on α-tocopherol and thiobarbituric acid reactive substance (TBARS) concentration in the rat liver, heart, muscles, and testes. Male Wistar rats (n=32) were divided into four groups (nonsupplemented, not trained—C; nonsupplemented, trained—CT; supplemented, not trained—E; supplemented and trained—ET). During the 14-day experimental period, 2 mg/d of vitamin E as α-tocopherol acetate was administered to the animals (groups E and ET). Rats in the training group (CT and ET) were subjected to 15 minutes of treadmill running each day. The α-tocopherol levels in rat tissues were assessed using high-performance liquid chromatography (HPLC). Lipid peroxides were determined by TBARS spectrophotometric method. α-Tocopherol had a significant impact on α-tocopherol concentration in all tissues. Training increased the α-tocopherol concentration in the heart and muscles but reduced it in the liver. Training also caused increased lipid peroxidation in the muscles, heart, and testes; but a higher α-tocopherol content in tissues reduced the TBARS level. The main finding of the study is that impaired α-tocopherol status and its adequate intake is needed to maintain optimal status to prevent damage to the skeletal and cardiac muscles as well as the testes in growing individuals.

scholarly journals Reperfusion endotoxin eye damage in experimental study

2015 ◽  
Vol 96 (5) ◽  
pp. 811-814 ◽  
Author(s):  
G A Drozdova ◽  
A F Samigullina ◽  
E A Nurgaleeva ◽  
A A Sorokin
Keyword(s):  
Lipid Peroxidation ◽  
Circulatory Arrest ◽  
Free Radical Oxidation ◽  
Thiobarbituric Acid ◽  
Male Rats ◽  
Integral Index ◽  
Radical Oxidation ◽  
Endogenous Intoxication ◽  
Eye Tissues ◽  
Peroxidation Processes

Aim. To evaluate the level of endogenous intoxication in the blood and the status of the lipid peroxidation processes in eye tissues of experimental animals after acute circulatory arrest. Methods. Chronic (5 weeks) pathophysiological experiments were performed in 72 nonlinear mature male rats, in which acute circulatory arrest was modeled by neurovascular heart bundle compression, followed by resuscitation. Endogenous intoxication intensity was assessed using endogenous intoxication integral index based on the detection of oligopeptides and low and medium molecular weight substances. Free radical oxidation in eye tissues was assessed by determining the level of 2’-thiobarbituric acid-reactive products, as well as by chemiluminescence. The condition of antioxidant system was investigated by catalase activity features and reduced glutathione level. Results. There was a significant two-phased increase of endogenous intoxication index during the whole postoperative period. Fe2+-induced chemiluminescence with subsequent accumulation of lipid peroxidation secondary metabolites, as well as increased activity of catalase protective system both in early stages (days 1-3) and on the second week were revealed in eye tissues. A positive correlation of endogenous intoxication integral index and the level of 2’-thiobarbituric acid-reactive products on the first day after resuscitation was revealed. Conclusion. Endogenous intoxication syndrome is formed after the acute circulatory arrest with maximal levels of endogenous toxins in the systemic circulation on 1-3rd and on 10-14th day after resuscitation, as well as with significant intensification of lipid peroxidation processes in eye tissues at the same period.

scholarly journals EFFECTS OF GLYPROLINES ON THE BEHAVIOR OF RATSIN THE PORSOLTTEST AND EXPERIMENTALLYINDUCED SOCIALS

2020 ◽  
Vol 10 (4) ◽  
pp. 31-34
Author(s):  
Marina Samotrueva ◽  
Anna Yasenyavskaya ◽  
Alexandra Tsibizova ◽  
Liudmila Andreeva ◽  
Nikolai Myasoedov
Keyword(s):  
Anxiety Disorders ◽  
Social Stress ◽  
White Male ◽  
Male Rats ◽  
Behavioral Analysis ◽  
Depression And Anxiety ◽  
Sensory Contact ◽  
Porsolt Test ◽  
Type Of Behavior ◽  
Behavior Of Rats

In this work we studied the effect of glyprolines on the behavior of rats under conditions of experimental social stress. White male rats 6-8 months of age were used in the experiment. Throughout the experiment all animals were kept in standard-barrier conditions. The effect of glyprolines on the behavior of white male rats was studied on the model of social stress, implying that the animals are kept in conditions of a constant sensory contact. Glyprolines (Selank, Pro-Gly-Pro, Pro-Gly-Pro-Leu) were injected to the animals Intraperitoneally at a dose of 100 μg/kg/day within 20 days. Porsolt test was employed to carry out behavioral analysis in the animals. The outcomes revealed that regardless of the type of behavior (aggressive and submissive) in all the animals depression and anxiety disorders were developed, whereas Selank, Pro-Gly-Pro, Pro-Gly-Pro-Leu facilitated their alleviation. Thus, our experiment has demonstrated a psychomodulatory effect of the glyprolines.

scholarly journals Influence of neuropeptides on psychoemotional state under conditions of “social” stress

2020 ◽  
pp. 37-45
Author(s):  
Anna L. Yasenyavskaya ◽  
Marina A. Samotrueva ◽  
Aleksandra A. Tsibizova ◽  
Nikolai F. Myasoedov ◽  
Lyudmila A. Andreeva
Keyword(s):  
Social Stress ◽  
White Male ◽  
The Body ◽  
Male Rats ◽  
Behavioral Reactions ◽  
Sensory Contact ◽  
White Rats ◽  
Situational Anxiety ◽  
Psychoemotional State

Objective: experimental study of the influence of neuropeptides on the psychoemotional state of white male rats exposed to “social” stress. Materials and methods. A study of the psychomodulatory effect of neuropeptides (Semax, Selank, ACTH(6-9)-Pro-Gly-Pro, Pro-Gly-Pro, Pro-Gly-Pro-Leu) under conditions of experimental “social” stress was performed on white non-linear male rats. The studied neuropeptides were synthesized at the Institute of Molecular Genetics of the National Research Center “Kurchatov Institute”. The study of the effect of neuropeptides on the behavior of male white rats was carried out on the model of “social” stress, the methodological reception of which is the constant residence of animals under conditions of sensory contact in order to form aggressive and submissive types of behavior. The effect of neuropeptides on the psychoemotional state of white rats under conditions of “social” stress was assessed on the basis of studying the behavior of animals in the tests “Elevated сruciform maze” and “Porsolt”. The “Elevated cruciform maze” test makes it possible to study the behavior of animals under conditions of variable stress (with a free choice of comfortable conditions) and allows assessing: the level of anxiety of the animal; symptoms of neurological deficiency. The test “Porsolt” provides an assessment of changes in the motor activity of animals and allows you to study the severity of the depressive state. Results. It was found that the effect of “social” stress on the behavior of male rats contributed to an increase in situational anxiety and the appearance of depressive-like behavioral reactions. It has been proven that the introduction of neuropeptides (Semax, Selank, ACTH(6-9)-Pro-Gly-Pro, Pro-Gly-Pro, Pro-Gly-Pro-Leu) helps to reduce increased anxiety in animals, both with aggressive and submissive types of behavior under conditions of “social” stress thereby providing a psychomodulatory effect. Conclusion. This study expands the understanding of the regulatory role of neuropeptides in the body, demonstrating the possibility of their influence on the psychoemotional state.

Effects of co-exposure to lead and zinc on redox status, kidney variables, and histopathology in adult albino rats

2018 ◽  
Vol 34 (7) ◽  
pp. 469-480 ◽  
Author(s):  
Ahlem Soussi ◽  
Manel Gargouri ◽  
Abdelfattah El Feki
Keyword(s):  
Antioxidant Activities ◽  
Kidney Tissue ◽  
Toxic Metal ◽  
Kidney Injury ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Male Rats ◽  
Albino Rats ◽  
Corrective Effect ◽  
Wide Range

Lead (Pb) is a toxic metal that induces a wide range of biochemical and physiological effects in humans. Oxidative damage has been proposed as a possible mechanism involved in Pb toxicity. The current study was carried out to evaluate the antioxidant activities of zinc (Zn) supplement against lead acetate–induced kidney injury in rats. In this study, adults male rats were treated for 15 days with Pb (0.344 g/kg body weight (bw)) associated or not with Zn (10 mg/kg bw). Our study showed that supplementation with Zn prevented renal dysfunction as indicated by plasma biomarkers (urea, uric acid, creatinine, lactate dehydrogenase, and alkaline phosphatase levels) and oxidative stress–related parameters (thiobarbituric acid reactive substances, protein carbonyl, advanced oxidation protein product, superoxide dismutase, catalase, glutathione peroxidase, and vitamins (A, E)) in kidney tissue. The corrective effect of Zn on Pb-induced kidney nephrotoxicity recovered normal kidney histology. Overall, this study indicates that Zn alleviated the toxic effects of this heavy metal on renal tissue, suggesting its role as a potential antioxidant and nephroprotective agent.

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