ISSLEDOVANIE AUTOANTITEL K KOLLAGENU RAZLIChNYKh TIPOVV SYVOROTKE KROVI BOL'NYKh S DEGENERATIVNO-DISTROFIChESKIMIZABOLEVANIYaMI TAZOBEDRENNYKh SUSTAVOV

2003 ◽  
Vol 10 (1) ◽  
pp. 73-77
Author(s):  
T V Buylova ◽  
E V Puchenkina ◽  
M Yu Lebedev
Keyword(s):  
Solid Phase ◽  
Clinical Manifestations ◽  
Femur Head ◽  
Aseptic Necrosis ◽  
Passive Hemagglutination ◽  
Immunoenzyme Analysis ◽  
High Level ◽  
Articular Pathology ◽  
Reliable Increase ◽  
Marked Tendency

The level of autoantibody (AAB) to collagen was studied in serum of patients with degenerative dystrophic hip joint diseases: deforming coxarthrosis of I, II, III degree, aseptic necrosis of femur head of III, IV degree and cystic remodeling of articular ends of II, III degree. In 123 patients level of AAB to general determinants of collagens was detected using reaction of passive hemagglutination. In 24% of patients high diagnostically significant of AAB titers to collagen were determined. Correlation of AAB level and general determinants of various collagen types as well as the type of articular pathology were studied. In 62 patients AAB level to collagen of I, II, III and IV types was detected using solid phase immunoenzyme analysis. High level of AAB to collagen of I, II types was shown. In patients with aseptic necrosis reliable increase of AAB level to collagen of I (osseous) type and marked tendency to the increase of AAB level to collagen of II (cartilagenous) type was detected. In patients with cystic remodeling reliable increase of AAB level to collagen of II type and tendency to the increase of AAB level to collagen of I type was observed. Strong correlation between AAB level to collagen of II type and clinical manifestations of pathology was determined.

scholarly journals Expression of the apoptosis-releated genes in patients with newly diagnosed chronic lymphocytic leukemia in clinical data context

2018 ◽  
Vol 17 (2) ◽  
pp. 41-46 ◽  
Author(s):  
S. G. Zakharov ◽  
A. K. Golenkov ◽  
A. V. Misyurin ◽  
E. V. Kataeva ◽  
A. A. Rudakova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Clinical Manifestations ◽  
Multivariate Regression Analysis ◽  
Lymphocytic Leukemia ◽  
Newly Diagnosed ◽  
Expression Of Genes ◽  
Gene Level ◽  
Genes Encoding ◽  
Group Ii ◽  
High Level

Introduction.The given data of fundamental studies of apoptosis processes in B-cell lymphocytic leukemia (B-CLL) testifies about the complexity and variety of mechanisms affecting the kinetics of normal cells and tumor lymphocytes in this disease. It is important to study the severity of clinical manifestations of the disease depending on the expression of the genes that modulate apoptosis.The purposeof the study is to compare the activity of genes encoding apoptosis modulators, the cell cycle and cancer-testicular PRAME protein with clinical manifestations of the disease in primary patients with B-CLL.Materials and methods.The level of expression of the proapoptotic genes FAS, TRAIL, TNFR2, DR4/5 and DR3, as well as the HSP27, XIAP genes, blocking apoptosis was determined in 23 patients with newly diagnosed chronic B-CLL. In addition, expression of genes TP53 and P21 and cancer-testis gene PRAME are tested.Results.According to the multivariate regression analysis, the FAS gene expression in the onset of the disease had the greatest impact on the clinical characteristics of the disease. In this connection, the patients were divided into groups with normal (group) and low gene level (group II). A low level of FAS expression (Me 387 %) was associated with stage II disease (p = 0.03), a large number of lympho cytes (p = 0.001), fewer erythrocytes (p = 0.08), and a lower level of TNFR2 gene expression (p = 0.08), high level of expression of XIAP, HSP27, P21. Overall, the anti-apoptotic potential in Group II patients was higher, which was accompanied by more pronounced clinical manifestations of the disease.Conclusions.The increased anti-apoptotic potential of tumor lymphocytes in newly diagnosed B-CLL is accompanied by a larger tumor mass and greater clinical and hematological manifestation of the disease.

Design Techniques for Microfluidic Devices Implementation Applicable to Chemical Analysis Systems

2019 ◽  
pp. 195-222 ◽  
Author(s):  
Reinaldo Lucas dos Santos Rosa ◽  
Antonio Carlos Seabra
Keyword(s):  
Chemical Analysis ◽  
Solid Phase ◽  
Microfluidic Devices ◽  
Water Quality Monitoring ◽  
Analysis Process ◽  
Micro Total Analysis Systems ◽  
Total Analysis ◽  
On Chip ◽  
Separation Cell ◽  
High Level

This chapter provides a guide for microfluidic devices development and optimization focused on chemical analysis applications, which includes medicine, biology, chemistry, and environmental monitoring, showing high-level performance associated with a specific functionality. Examples are chemical analysis, solid phase extraction, chromatography, immunoassay analysis, protein and DNA separation, cell sorting and manipulation, cellular biology, and mass spectrometry. In this chapter, most information is related to microfluidic devices design and fabrication used to perform several steps concerning chemical analysis, process preparation of reagents, samples reaction and detection, regarding water quality monitoring. These steps are especially relevant to lab-on-chip (LOC) and micro-total-analysis-systems (μTAS). μTAS devices are developed in order to simplify analytical chemist work, incorporating several analytical procedures into flow systems. In the case of miniaturized devices, the analysis time is reduced, and small volumes (nL) can be used.

Assessment of Redox Conditions in the Near Field of Nuclear Waste Repositories: Application to the Swiss high-level and intermediate level waste disposal concept

2003 ◽  
Vol 807 ◽  
Author(s):  
Paul Wersin ◽  
Lawrence H. Johnson ◽  
Bernhard Schwyn
Keyword(s):  
Solid Phase ◽  
Near Field ◽  
Intermediate Level ◽  
Redox Conditions ◽  
High Level Waste ◽  
Redox Potentials ◽  
Spent Fuel ◽  
Reducing Conditions ◽  
High Level ◽  
Waste Repositories

ABSTRACTRedox conditions were assessed for a spent fuel and high-level waste (SF/HLW) and an intermediate-level waste (ILW) repository. For both cases our analysis indicates permanently reducing conditions after a relatively short oxic period. The canister-bentonite near field in the HLW case displays a high redox buffering capacity because of expected high activity of dissolved and surface-bound Fe(II). This is contrary to the cementitious near field in the ILW case where concentrations of dissolved reduced species are low and redox reactions occur primarily via solid phase transformation processes.For the bentonite-canister near field, redox potentials of about -100 to -300 mV (SHE) are estimated, which is supported by recent kinetic data on U, Tc and Se interaction with reduced iron systems. For the cementitious near field, redox potentials of about -200 to -800 mV are estimated, which reflects the large uncertainties related to this alkaline environment.

scholarly journals Clinical Significance of Galactose-Deficient IgA1 by KM55 in Patients with IgA Nephropathy

2019 ◽  
Vol 44 (5) ◽  
pp. 1196-1206 ◽  
Author(s):  
Kai Zhang ◽  
Qiongqiong Li ◽  
Yaru Zhang ◽  
Wenya Shang ◽  
Li Wei ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Clinical Significance ◽  
Clinical Manifestations ◽  
Renal Tissue ◽  
Healthy Controls ◽  
Activity Assessment ◽  
Activation Products ◽  
The Difference ◽  
Positive Correlations ◽  
High Level

Background: Aberrant galactose-deficient IgA1 molecules (Gd-IgA1) are important causal factors in IgA nephropathy (IgAN); however, the detection of Gd-IgA1 in IgAN is complicated and instable. A monoclonal antibody, KM55, which specifically recognizes Gd-IgA1 has been developed. In the present study, we further explored the clinical significance of Gd-IgA1 using KM55. Methods: In this study, we enrolled 75 patients with IgAN and 80 healthy controls and detected the plasma Gd-IgA1 levels using the KM55 ELISA method. We also stained ­mesangial Gd-IgA1 deposition using KM55. Results: We observed that the levels of plasma Gd-IgA1 in IgAN patients were elevated compared to the corresponding levels of healthy controls. Patients were divided into 2 groups based on the median of Gd-IgA1. Patients with high Gd-IgA1 levels had significantly higher levels of uric acid (UA) and IgA. The other clinical manifestations demonstrated that there were no differences in age, sex, blood pressure, initial proteinuria, hematuria, estimated glomerular filtration rate and Oxford pathological classification between the 2 groups of patients. In addition, positive correlations were observed between Gd-IgA1 and Bb, C3a, C4d and MAC. Mesangial Gd-IgA1 was positive in IgAN but negative in the normal renal tissue adjacent to neoplasm. We next analyzed the correlation between plasma Gd-IgA1 and mesangial Gd-IgA1 deposition. The results showed that a high level of plasma Gd-IgA1 was related to the deposition of mesangial Gd-IgA1, although the difference was not significant. Conclusion: We verified the elevated level of plasma and ­mesangial Gd-IgA1 in patients with IgAN by KM55, which provided an alternative, easy, and reliable tool for diagnosis and activity assessment of IgAN. The level of plasma Gd-IgA1 positively correlated with levels of UA, total IgA levels, and complement activation products.

Chronic active hepatitis with high level of anti-ds-DNA detected by a solid phase radioimmunoassay

1982 ◽  
Vol 1 (3) ◽  
pp. 208-211 ◽  
Author(s):  
A. Peretz ◽  
F. Mascart-Lemone ◽  
G. Nuttin ◽  
J. P. Famaey
Keyword(s):  
Chronic Active Hepatitis ◽  
Solid Phase ◽  
Solid Phase Radioimmunoassay ◽  
High Level

Doping and Alloying of Hydrogenated Amorphous Germanium Films Prepared by DC-Magnetron Sputtering

1993 ◽  
Vol 297 ◽  
Author(s):  
T. DrÜsedau ◽  
A. Annen ◽  
B. SchrÖder
Keyword(s):  
Solid Phase ◽  
Dark Conductivity ◽  
Concentration Addition ◽  
Phase Concentration ◽  
N Doping ◽  
Solid Phase Concentration ◽  
Donor States ◽  
B Doping ◽  
Si Content ◽  
High Level

In this paper we present a detailed study of doping and alloying effects in a-Ge:H due to the incorporation of phosphorus, nitrogen, oxygen, boron, carbon and silicon. The film characterization was performed by means of quantitative analysis , optical absorption in the band and subband range and conductivity measurements. N and O were found to form donor states resulting in an increase of the dark conductivity up to five orders of magnitude. The maximum value of 0.3 (Ωcm)-1 has been obtained by N-doping of about 1 at% solid phase concentration, which exceeds the effect of P doping. B doping causes a decrease of the dark conductivity to a minimum of 10-6 (Ωcm)-1 at 150 ppm solid phase concentration. Addition of Si and C up to concentrations of about 5 at % does not influence the subgap absorption, where best values of E0<50 meV and α(0.6 eV)<10 cm-1 were obtained for the Urbach energy and midgap absorption, respectively. A Si content of 1 at % decreases the dark conductivity, whereas the normalized photoconductivity remains at a high level of ŋμτ=10-5 cm2V-1.

Advantage and disadvantage of laparoscopic choledochal cyst excision

2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Thanh Xuân Nguyễn
Keyword(s):  
Choledochal Cyst ◽  
Clinical Manifestations ◽  
Postoperative Outcome ◽  
Epidemiological Data ◽  
Postoperative Treatment ◽  
Biliary Leakage ◽  
Cyst Excision ◽  
Operation Duration ◽  
Tu 5 ◽  
High Level

Tóm tắt Đặt vấn đề: Đánh giá những thuận lợi và khó khăn trong phẫu thuật nội soi cắt nang ống mật chủ. Phương pháp nghiên cứu: Gồm 70 người bệnh nang ống mật chủ được chẩn đoán và điều trị bằng phẫu thuật nội soi tại Bệnh viện Trung ương Huế từ tháng1/2012 đến tháng 12/2017. Kết quả: Thuận lợi: Thời gian phẫu thuật trung bình (219,79 ± 64,88 phút) ngắn hơn so với thời gian phẫu thuật của nhiều nghiên cứu khác. Không có người bệnh tái khám sau mổ từ 10 ngày đến 3 tháng có đánh giá kết quả điều trị trung bình hoặc xấu. 94,1% người bệnh được rút dẫn lưu trong vòng 1 - 4 ngày sau mổ. 71,4% số người bệnh nằm viện sau mổ từ 5 - 10 ngày. Có 94,9% người bệnh hài lòng với kết quả điều trị sau mổ từ 10 ngày đến 3 tháng. Khó khăn: Có 5 trường hợp dò mật sau mổ trong đó 3 trường hợp theo dõi sau 5 ngày người bệnh ổn định, 2 trường hợp dò mật kéo dài phải mổ lại để làm lại miệng nối. Có 2 người bệnh phải truyền máu do kích thước nang lớn, dính nhiều tổ chức xung quanh và thời gian phẫu thuật kéo dài. Kết luận: Phẫu thuật nội soi trong điều trị cắt nang ống mật chủ là phương pháp an toàn, hiệu quả và khả thi ở các trung tâm y tế lớn, tuy nhiên đòi hỏi cao về kỹ năng của phẫu thuật viên, trình độ gây mê cũng như trang thiết bị phòng mổ. Abstract Introduction: Evaluating the advantage and disadvantage of the laparoscopic choledochal cyst excision. Material and Methods: We analysed 70 patients who were treated at the Hue Central Hospital from January 2012 to December 2017 with statistical analysis of epidemiological data, clinical manifestations, diagnosis, treatment and postoperative outcome. Results: Advantages: Average operation duration (219.79 ± 64.88 minutes) was shorter than typical intervention. Postoperative treatment results were evaluated from 10 days to 3 months after surgery: No average or bad result. 94.1% of patients were withdrawn drains within 1- 4 days after surgery. There were 71.4% of patients who hospitalized from 5 - 10 days postoperatively and 94.9% of patients were satisfied with the results. Disadvantages: 5 cases of postoperative biliary leakage were found, including 3 cases that were stabilized after 5 days of medical treatment and 2 cases requiring surgical intervention for prolonged anastomotic leakage. Blood transfusions were seen in 2 cases because the large size of the cyst, adherences to surrounding tissues and prolonged operation duration. Conclusion: Laparoscopic surgery for choledochal cyst resection is a safe and effective method. However, it requires a high level of anaesthesia and operating room equipment. Keyword: Laparoscopic choledochal cyst excision, Advantage and disadvantage.

Nonideality Effects on the Ion Exchange Behavior of the Zeolite Mineral Clinoptilolite

1990 ◽  
Vol 212 ◽  
Author(s):  
Roberto T. Pabalan
Keyword(s):  
Ion Exchange ◽  
Solid Phase ◽  
Solution Concentration ◽  
Nuclear Waste Repository ◽  
Thermodynamic Models ◽  
Diagenetic Alteration ◽  
Energy Models ◽  
High Level Nuclear Waste ◽  
High Level ◽  
Induced Changes

ABSTRACTThe presence of laterally-extensive zones of zeolitized tuff underlying the proposed high-level nuclear waste repository at Yucca Mt., Nevada, has focused attention on the potential role of zeolite minerals, particularly clinoptilolite, in sorbing radionuclides and thereby retarding their migration. Ion exchange between zeolites and aqueous solutions depends on factors including compositions of the aqueous and zeolite phases and solution concentration. In addition, the thermodynamic stability of zeolite minerals and their susceptibility to diagenetic alteration also depend on aqueous and solid phase compositions. Therefore, spatial variations in zeolite compositions which have been observed at Yucca Mt., as well as natural or repository-induced changes in groundwater chemistry, may result in variations in the effectiveness of the zeolite minerals as retardation agents.Ion exchange experiments were conducted to obtain isotherm data and to evaluate the use of thermodynamic models in describing and predicting the solid solution and ion exchange properties of clinoptilolite. The experimental data were interpreted using excess Gibbs energy models for the aqueous solution and zeolite phases to account for nonideality in the system. The results indicate that the thermodynamic models allow predictions of clinoptilolite ion exchange behavior at ionic strengths and relative concentrations for which data are absent, and provide a foundation for the interpretation of ion exchange equilibria in multicomponent geochemical systems.

Stress and Dopant Activation in Solid Phase Crystalized Si Films

1999 ◽  
Vol 558 ◽  
Author(s):  
A. Kaan Kalkan ◽  
Stephen J. Fonash
Keyword(s):  
Tensile Stress ◽  
Defect Density ◽  
Solid Phase ◽  
Structural Defects ◽  
Raman Shift ◽  
Solid Phase Crystallization ◽  
Dopant Activation ◽  
Defect Creation ◽  
Si Films ◽  
High Level

ABSTRACTDefect creation mechanisms during solid phase crystallization (SPC) of Si thin films were investigated with PECVD amorphous precursor samples produced with various deposition temperatures and thicknesses. These precursor films were implanted with dopant and then crystallized to obtain both SPC and dopant activation. The doping efficiency was found to decrease with the tensile stress level as measured by Raman shift. The stress shows a decrease as the precursor deposition temperature and thickness are lowered. Furthermore, a lower level of stress is induced by rapid thermal annealing when the annealing temperature is high enough to soften the glass substrate on which the films were deposited. We show that by control of stress during the SPC step, intragrain defect density can be lowered and electronic quality of the resulting polycrystalline Si films can be improved. Based on these observations, we propose the following tentative model to explain the defect creation: during SPC, tensile stress evolution is considered to result from the volumetric contraction of Si film when it transforms from the amorphous to crystalline phase. This contraction is retarded by the substrate, which imposes a tensile stress on the film. A high level of stress leads to formation of structural defects inside the grains of the resulting polycrystalline material. These defects trap carriers or complex with the dopant reducing doping efficiency.

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