scholarly journals Extraction by Dry Rendering Methode and Characterization Fish Oil of Catfish Viscera Fat by Product of Smooked Fish Processing

2017 ◽  
Vol 19 (3) ◽  
pp. 196
Author(s):  
Kamini Kamini ◽  
Pipih Suptijah ◽  
Joko Santoso ◽  
Sugeng Heri Suseno
Keyword(s):  
Heavy Metal ◽  
Fatty Acid ◽  
Fish Oil ◽  
Fatty Acid Profile ◽  
Profile Analysis ◽  
Fatty Acid Content ◽  
Oil Quality ◽  
Fish Processing ◽  
Yield Value ◽  
By Products

Abstract<br />The catfish viscera fat, is cathfish processing by-products, has potential to be used as a source of raw<br />material for production of fish oil. This study aimed to analyze the value of proximate, heavy metal content<br />and fatty acid profile of catfish viscera fat (Pangasius hypopthalmus) and characterized fish oil extracted by<br />dry rendering in various temperature and time than compared it to fish oil extracted by stove heating to<br />obtain the best treatment. Proximate, heavy metal residue, and the fatty acid profile analysis were conducted<br />for characterizing catfish viscera fat. Fish oil extraction was conducted by dry rendering in various<br />temperatures of 50, 60, 70, 80 °C for 1, 2, and 3 hours. Fish oil quality was determined by the chemical<br />characteristics i.e. PV, FFA, anisidin and TOTOX. The results of the study showed that fat content of catfish<br />fat viscera was 88.19 %, the heavy metals content was below SNI standart to be consumed, and fatty acid<br />profile composition was SFA&gt;MUFA&gt;PUFA. The highest fatty acid content was oleic acid. The best fish oil<br />quality was resulted on temperature extraction of 50°C for 2 hours with yield value, PV, FFA, anisidin, and<br />TOTOX were 45.17 %, 2.77 meq/kg, 0.83 %, 2.86 meq/kg, 8.39 meq/kg respectively. This result was not<br />significantly different with fish oil extracted by the stove heating expect for yield and PV were 80.11% and<br />6.52 meq/kg, respectively.<br /><br />

scholarly journals Extraction by Dry Rendering Methode and Characterization Fish Oil of Catfish (Pangasius hypopthalmus) Viscera Fat by Product of Smooked Fish Processing

2016 ◽  
Vol 19 (3) ◽  
pp. 196 ◽  
Author(s):  
Kamini Kamini ◽  
Pipih Suptijah ◽  
Joko Santoso ◽  
Sugeng Heri Suseno
Keyword(s):  
Heavy Metal ◽  
Fatty Acid ◽  
Fish Oil ◽  
Fatty Acid Profile ◽  
Profile Analysis ◽  
Fatty Acid Content ◽  
Oil Quality ◽  
Raw Material ◽  
Yield Value ◽  
By Products

The catfish viscera fat, is cathfish processing by-products, has potential to be used as a source of<br />raw material for production of fish oil. This study aimed to analyze the value of proximate, heavy metal<br />content and fatty acid profile of catfish viscera fat (Pangasius hypopthalmus) and characterized fish oil<br />extracted by dry rendering in various temperature and time than compared it to fish oil extracted by stove<br />heating to obtain the best treatment. Proximate, heavy metal residue, and the fatty acid profile analysis<br />were conducted for characterizing catfish viscera fat. Fish oil extraction was conducted by dry rendering<br />in various temperatures of 50, 60, 70, 80 °C for 1, 2, and 3 hours. Fish oil quality was determined by the<br />chemical characteristics i.e. PV, FFA, anisidin and TOTOX. The results of the study showed that fat content<br />of catfish fat viscera was 88.19 %, the heavy metals content was below SNI standart to be consumed, and fatty<br />acid profile composition was SFA&gt;MUFA&gt;PUFA. The highest fatty acid content was oleic acid. The best fish<br />oil quality was resulted on temperature extraction of 50°C for 2 hours with yield value, PV, FFA, anisidin,<br />and TOTOX were 45.17 %, 2.77 meq/kg, 0.83 %, 2.86 meq/kg, 8.39 meq/kg respectively. This result was not<br />significantly different with fish oil extracted by the stove heating expect for yield and PV were 80.11% and<br />6.52 meq/kg, respectively.<br /><br />

scholarly journals PROFIL ASAM LEMAK MINYAK IKAN SELAR (Selaroides leptolesis) DENGAN BERBAGAI LEVEL PENAMBAHAN BUBUK KEMANGI (Ocimum bacilicum)

2020 ◽  
Vol 2 (2) ◽  
pp. 147-152
Author(s):  
Minarny Gobel ◽  
Anita Treisya Aristawati ◽  
Steven Yoputra
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Profile Analysis ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Animal Husbandry ◽  
Monounsaturated Fatty Acids ◽  
Chemistry Laboratory ◽  
Gas Chromatography Mass Spectrometry

This study aimed to know the profile and composition of fatty acid consisting of polyunsaturated fatty acid content (PUFA), monounsaturated fatty acid (MUFA), and saturated fatty acid (SFA) in selar fish oil (selaroides leptolesis) with various levels of basil powder (Ocimum basilicum)². The research was conducted at the Animal Product Technology Laboratory, Department of Animal Husbandry, Faculty of Animal Husbandry and Fisheries of Tadulako University. The research using 4 treatments and 3 replications with treatment M0 = 10 ml fish oil + 0% Basil Powder, M1 = 10 ml fish oil + 2% Basil Powder, M2 = 10 ml fish oil + 4% Basil Powder, M3 = 10 ml fish oil + 6% Basil Powder, each treatment repeated 3 times so that there are 12 experimental units. Fatty acid profile analysis using GCMS (gas chromatography-mass spectrometry), conducted in Organic Chemistry Laboratory of Faculty of Mathematics and Natural Sciences UGM Jogjakarta. The results of this study showed that 10 ml fish oil with various levels of basil powder added 18 types of fatty acids belonging to polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA), and saturated fatty acids (SFA). It can be concluded that the addition of 6% powder of basil can defend SFA and increase MUFA and PUFA.

scholarly journals Biodiesel production from waste fish oil with high free fatty acid content from Moroccan fish-processing industries

2018 ◽  
Vol 27 (2) ◽  
pp. 249-255 ◽  
Author(s):  
K. Kara ◽  
F. Ouanji ◽  
El M. Lotfi ◽  
M. El Mahi ◽  
M. Kacimi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Fish Oil ◽  
Free Fatty Acid Content ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Biodiesel Production ◽  
High Free Fatty Acid ◽  
Fish Processing

scholarly journals Fish oil quality of by-product (fish skin) from swangi fish

2015 ◽  
Vol 17 (3) ◽  
pp. 232
Author(s):  
La Ode Huli ◽  
Sugeng Heri Suseno ◽  
Joko - Santoso
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Fatty Acid Profile ◽  
Oil Quality ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Extraction Temperature ◽  
Fish Skin ◽  
Fatty Acid Compositions

The skin of swangi fish is a potential fish skin to be produced for fish oil. The objectives of this research were aimed to determine the yield and the best quality of fish oil and also to compare fatty acid profile of the fish according to different extraction methods. Fish oil extractions were used by wet rendering method with extraction temperatures of 60, 70, 80, 90, 100°C for 20, 30, and 40 minutes. Fish oil quality was determined by the chemical oil characteristics i.e. PV, FFA, AV, anisidin, and TOTOX. Fatty acid profile was analyzed using gas chromatography (Shimadzu). The results of the study showed that the highest fish oil yield in each treatment was obtained extraction temperature of 60°C for 30 minutes with percentage of 0.33, (70°C for 30 minutes) 0.46, (80°C for 30 minutes) 1.23, (90°C for 20 minutes) 1.14 and (100°C for 20 minutes) 0.84. These values were lower compare to Bligh &amp; Dyer and Soxhlet methods. Then, the best fish oil quality was resulted on temperature extraction of 60°C for 30 minutes with PV, FFA, anisidin, AV, and TOTOX were 9.17 meq/kg, 6.92%, 13,77 mg KOH/g, 0.86 meq/kg and 19.19 meq/kg, respectively. FUFA fatty acid compositions of swangi skin fish oil especially EPA and DHA in wet rendering method were gained 0.73% and 2.53%, respectively. These results were lower than Bligh &amp; Dyer method which was consisted of 3.66% (EPA), and 13.29% (DHA) and also Soxhlet extraction method with value of EPA was 2.78% and DHA was 9.62%.<br />Keywords: EPA, extraction temperature, DHA, fish oil quality, fish skin

scholarly journals Enzyme-Assisted Extraction of Fish Oil from Whole Fish and by-Products of Baltic Herring (Clupea harengus membras)

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1811
Author(s):  
Ella Aitta ◽  
Alexis Marsol-Vall ◽  
Annelie Damerau ◽  
Baoru Yang
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Crude Oil ◽  
Proteolytic Enzymes ◽  
Clupea Harengus ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Baltic Herring ◽  
By Products

Baltic herring (Clupea harengus membras) is one of the most abundant commercially caught fish species from the Baltic Sea. Despite the high content of fat and omega-3 fatty acids, the consumption of Baltic herring has decreased dramatically over the last four decades, mostly due to the small sizes and difficulty in processing. At the same time there is an increasing global demand for fish and fish oil rich in omega-3 fatty acids. This study aimed to investigate enzyme-assisted oil extraction as an environmentally friendly process for valorizing the underutilized fish species and by-products to high quality fish oil for human consumption. Three different commercially available proteolytic enzymes (Alcalase®, Neutrase® and Protamex®) and two treatment times (35 and 70 min) were investigated in the extraction of fish oil from whole fish and by-products from filleting of Baltic herring. The oil quality and stability were studied with peroxide- and p-anisidine value analyses, fatty acid analysis with GC-FID, and volatile compounds with HS-SPME-GC-MS. Overall, longer extraction times led to better oil yields but also increased oxidation of the oil. For whole fish, the highest oil yields were from the 70-min extractions with Neutrase and Protamex. Protamex extraction with 35 min resulted in the best fatty acid composition with the highest content of eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) but also increased oxidation compared to treatment with other enzymes. For by-products, the highest oil yield was obtained from the 70-min extraction with Protamex without significant differences in EPA and DHA contents among the oils extracted with different enzymes. Oxidation was lowest in the oil produced with 35-min treatment using Neutrase and Protamex. This study showed the potential of using proteolytic enzymes in the extraction of crude oil from Baltic herring and its by-products. However, further research is needed to optimize enzymatic processing of Baltic herring and its by-products to improve yield and quality of crude oil.

scholarly journals Establishment of a Patient-Derived Xenograft Model of Colorectal Cancer in CIEA NOG Mice and Exploring Smartfish Liquid Diet as a Source of Omega-3 Fatty Acids

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 282
Author(s):  
Helle Samdal ◽  
Lene C Olsen ◽  
Knut S Grøn ◽  
Elin S Røyset ◽  
Therese S Høiem ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acid ◽  
Fish Oil ◽  
Fatty Acid Content ◽  
Xenograft Model ◽  
Liquid Diet ◽  
Lymphoid Cells ◽  
Omega 3 ◽  
Pdx Model ◽  
Nog Mice

Cancer patient-derived xenografts (PDXs) better preserve tumor characteristics and microenvironment than traditional cancer cell line derived xenografts and are becoming a valuable model in translational cancer research and personalized medicine. We have established a PDX model for colorectal cancer (CRC) in CIEA NOG mice with a 50% engraftment rate. Tumor fragments from patients with CRC (n = 5) were engrafted in four mice per tumor (n = 20). Mice with established PDXs received a liquid diet enriched with fish oil or placebo, and fatty acid profiling was performed to measure fatty acid content in whole blood. Moreover, a biobank consisting of tissue and blood samples from patients was established. Histology, immunohistochemistry and in situ hybridization procedures were used for staining of tumor and xenograft tissue slides. Results demonstrate that key histological characteristics of the patients’ tumors were retained in the established PDXs, and the liquid diets were consumed as intended by the mice. Some of the older mice developed lymphomas that originated from human Ki67+, CD45+, and EBV+ lymphoid cells. We present a detailed description of the process and methodology, as well as possible issues that may arise, to refine the method and improve PDX engraftment rate for future studies. The established PDX model for CRC can be used for exploring different cancer treatment regimes, and liquid diets enriched with fish oil may be successfully delivered to the mice through the drinking flasks.

scholarly journals Fatty Acid Profile, Lipid Quality and Squalene Content of Teff (Eragrostis teff (Zucc.) Trotter) and Amaranth (Amaranthus caudatus L.) Varieties from Ethiopia

2021 ◽  
Vol 11 (8) ◽  
pp. 3590
Author(s):  
Endale Amare ◽  
Luca Grigoletto ◽  
Viviana Corich ◽  
Alessio Giacomini ◽  
Anna Lante
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Functional Foods ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
Gluten Free ◽  
Amaranthus Caudatus ◽  
Lipid Quality ◽  
Squalene Content

Teff and amaranth are gluten-free cereals with significant nutritional and health benefits. However, they are underutilized and known in limited areas of the world. The present study evaluated the fatty acid profile, crude fat, squalene content and lipid quality of seven teff (Eragrostis teff (Zucc.) Trotter) and three amaranth (Amaranthus caudatus L.) varieties from Ethiopia. The fat content ranged from 2.92 to 3.34% (averaging 3.06%) and from 8.28 to 9.21% (averaging 8.6%) for teff and amaranth, respectively. Linoleic, oleic and palmitic acid were predominant in both teff and amaranth, accounting for approximately 89 and 85% of total fatty acid content, respectively. The saturated to unsaturated fatty acids ratio ranged from 0.30 to 0.32 in teff and from 0.38 to 0.40 in amaranth. The parameters used to describe lipid quality, i.e., thrombogenicity and atherogenicity indices, show that teff was superior over amaranth, suggesting a preference for the former for healthy food formulation. The squalene content of white amaranth (486.54 mg/100 g DM) was significantly higher than that of the other two varieties (327.54 and 340.81 mg/100 g DM for red and brown amaranth, respectively). In general, both gluten-free crops should be exploited for their potential as ingredients for the development of novel functional foods.

scholarly journals Fatty acid positional distribution (sn-2 fatty acids) and phospholipid composition in Chinese breast milk from colostrum to mature stage

2018 ◽  
Vol 121 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Ke Wu ◽  
Runying Gao ◽  
Fang Tian ◽  
Yingyi Mao ◽  
Bei Wang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Fatty Acid Profile ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
Positional Distribution ◽  
Phospholipid Composition ◽  
Mature Stage ◽  
Feeding Time

AbstractThis study quantified the fatty acid profile with emphasis on the stereo-specifically numbered (sn) 2 positional distribution in TAG and the composition of main phospholipids at different lactation stages. Colostrum milk (n 70), transitional milk (n 96) and mature milk (n 82) were obtained longitudinally from healthy lactating women in Shanghai. During lactation, total fatty acid content increased, with SFA dominating in fatty acid profile. A high ratio of n-6:n-3 PUFA was observed as 11:1 over lactation due to the abundance of linoleic acid in Chinese human milk. As the main SFA, palmitic acid showed absolute sn-2 selectivity, while oleic acid, linoleic acid and α-linolenic acid, the main unsaturated fatty acids, were primarily esterified at the sn-1 and sn-3 positions. Nervonic acid and C22 PUFA including DHA were more enriched in colostrum with an sn-2 positional preference. A total of three dominant phospholipids (phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM)) were analysed in the collected samples, and each showed a decline in amount over lactation. PC was the dominant compound followed by SM and PE. With prolonged breast-feeding time, percentage of PE in total phospholipids remained constant, but PC decreased, and SM increased. Results from this study indicated a lipid profile different from Western reports and may aid the development of future infant formula more suitable for Chinese babies.

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