scholarly journals Identifikasi Bakteri Patogen (Vibrio spp. dan Salmonella spp.) yang Mengontaminasi Ikan Layang dan Bandeng di Pasar Tradisional

2021 ◽  
Vol 24 (1) ◽  
pp. 89-96
Author(s):  
Burhanuddin Ihsan
Keyword(s):  
Bile Salt ◽  
Determinative Bacteriology ◽  
Salmonella Spp ◽  
Vibrio Spp

Sumber konsumsi ikan masyarakat Kota Tarakan adalah ikan layang dan bandeng yang berasal dari pasar tradisional. Pengelolaan pasar tradisional yang ada di Kota Tarakan cukup memadai namun, tingkat kesehatan dan higienis lingkungan masih kurang baik, penanganan yang belum memadai memungkinkan banyak bakteri yang berkembang dan mengontaminasi ikan. Tujuan penelitian untuk mengetahui kontaminasi bakteri patogen (Vibrio spp. dan Salmonella spp.) pada ikan yang dijual di pasar tradisional kota Tarakan. Penelitian dilakukan dengan metode deskriptif dengan beberapa tahapan mulai dari observasi pasar, pengambilan sampel, isolasi bakteri dan identifikasi. Isolasi bakteri Vibrio menggunakan media selektif TCBS (Thiosulfate Citrate Bile Salt Sucrose) dan bakteri Salmonella menggunakan media SSA (Salmonella Shigella Agar) identifikasi bakteri Vibrio dan Salmonella yang berpedoman pada buku Bergey’s Manual of Determinative Bacteriology berdasarkan sifat morfologi dan kimiawi. Hasil identifikasi terdapat kontaminasi bakteri Vibrio spp. dan Salmonella spp. pada hasil perikanan yang dijual di pasar tradisional kota Tarakan khususnya ikan bandeng dan ikan layang.

scholarly journals Identification of Vibrio spp. causing vibriosis in spiny lobsters (Panulirus homarus L.) in Bengkulu marine temporary shelter ponds

2021 ◽  
Vol 869 (1) ◽  
pp. 012019
Author(s):  
R H Wibowo ◽  
Sipriyadi ◽  
W Darwis ◽  
N Susianti ◽  
S P Yudha ◽  
...  
Keyword(s):  
Bile Salt ◽  
Clinical Symptoms ◽  
Spiny Lobster ◽  
Bacterial Isolation ◽  
Determinative Bacteriology ◽  
Biochemical Tests ◽  
Internal Organs ◽  
Spiny Lobsters ◽  
Shrimp Fisheries ◽  
Vibrio Spp

Abstract Spiny lobster (Panulirus homarus) is one of the export commodities of the Indonesian fisheries subsector and an important component for shrimp fisheries in Indonesia. In the development of lobster cultivation, there are several obstacles, the presence of vibriosis infection caused by the pathogenic Vibrio bacteria. This study aimed to identify Vibrio spp. bacteria in spiny lobsters (P. homarus) reared in the marine cultivation ponds, Bengkulu, Indonesia. Clinical symptoms of lobsters infected with vibriosis are red spots on the uropod, pleopod, and abdominal parts. Bacterial isolation was conducted by isolated some internal organs in spiny lobsters, that are, gills, stomachs, haemolymph, and hepatopancreas. The result showed there are 5 isolates of Vibrio bacteria that coded by IN3, ST2, HA1, HP2, and HP3. These bacteria isolates were identified through their colony morphology and biochemical tests. Characterization on the Thiosulfate Citrate Bile Salt Sucrose (TCBS) medium showed that lobsters were infected with Vibrio species. Based on Bergey’s Manual of Determinative Bacteriology, Austin and Austin, the identification results showed that HA1 isolate was identified as Vibrio algynolyticus, IN3 isolate was identified as V. anguillarum, ST2 was identified as V. ordalii, HP2 in first lobster was identified as with V. algynolyticus that mostly in the hepatopancreas, and HP3 was identified as V. splendidus

NGHIÊN CỨU ĐẶC ĐIỂM GÂY BỆNH VÀ DỊCH TỄ CỦA BỆNH TÔM CHẾT SỚM (EMS) TẠI TỈNH QUẢNG BÌNH NHẰM ĐỀ XUẤT QUY TRÌNH CHẨN ĐOÁN VÀ PHÒNG TRỊNGHIÊN CỨU ĐẶC ĐIỂM DỊCH TỄ CỦA BỆNH TÔM CHẾT SỚM (EMS) TẠI TỈNH QUẢNG BÌNH NHẰM ĐỀ XUẤT QUY TRÌNH CHẨN ĐOÁN VÀ PHÒNG TRỊ

2015 ◽  
Vol 104 (5) ◽  
Author(s):  
Trần Thị Linh Giang ◽  
Dương Viết Phương Tuấn
Keyword(s):  
Bile Salt ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Spp

Nghiên cứu về Hội chứng chết sớm ở tôm thực hiện ở Quảng Bình với mục đích tìm hiểu đặc điểm gây bệnh của vi khuẩn Vibrio paraheamolyticus và đặc điểm dịch tể để khuyến cao cách phòng trị và có dự báo sớm làm giảm rủi roc ho nghề nuôi tôm. Có 120 phiếu và 91 mẫu tôm nghi bệnh được thu và nuôi cấy, tìm hiểu đặc điểm vi khuẩn này và phân tích gen để xác định độc tố, đồng thời nghiên cứu về đặc điểm dịch tễ của bệnh EMS ở 4 huyện, thành phố . Kết quả cho thấy rằng hơn 70% số mẫu nghi bệnh có kết quả dương tính, tần suất nhiễm bệnh cao 10 – 60,6 % và khác nhau ở các tháng và vụ nuôi, cao nhất vào tháng 4 – 7 DL, X2 = 1.60 (df = 4), với P < 0,05. Tôm nhiễm bệnh EMS có các biểu hiện các triệu chứng điển hình gan tuỵ và tỷ lệ chết cao đến 100% nếu không can thiệp kịp thời. Đặc điểm chung các loài vi khuẩn thuộc giống Vibrio: Gram âm, hình que thẳng hoặc hơi uốn cong, kích thước 0,3-0,5 x 1,4-2,6 μm, không hình thành bào tử và chuyển động nhờ một tiên mao hoặc nhiều tiên mao mảnh và yếm khí, hầu hết là oxy hoá và lên men trong môi trường O/F Glucose. Thiosulphate citrate bile salt agar (TCBS) là môi trường chọn lọc của Vibrio spp. Chúng mẫn cảm với Vibriostat 2,4 diamino-6,7 diisopropyl pteridine phosphate (0/129). Tỷ lệ V. parahaemolyticus gây bệnh tôm chết sớm.Vi khuẩn V. parahaemolyticus gây bệnh có đặc điểm cấu trúc gen khác biệt, nhiễm sắc thể tự điều chỉnh nằm ở vị trí 01. Từ những kết quả hình ảnh trên ta thấy được sự sai khác về trình tự gen DNA của mẫu W1, trình tự gen của mẫu này trùng hợp với trình tự gen DNA của Vibrio parahaemolyticus dùng đối chứng trên và sự sai khác về trình tự gen cũng đã thể hiện được khả năng gây bệnh của vi khuẩn V. parahaemolyticus. Các phản ứng với các loại kháng sinh có hiệu quả từ 8 – 45%, đều làm giảm số lượng tôm chết khi nhiễm, cao nhất là Baymet và Osamet, Olimos. Sử dụng chế phẩm Bokashi trầu với hiệu quả tốt nếu dùng từ đầu vụ và đến cuối vụ, với thành phần Eugenol, chavicol và chavibetol đã hạn chế sự phát triển của bệnh, kể cả những ao có mật độ V. parahaemolyticus cao nhưng ít có nguy cơ gây bệnh.

scholarly journals Quality assessment of Zeus faber (Peter’s fish) ovaries regularly commercialized for human consumption

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Filippo Giarratana ◽  
Graziella Ziino ◽  
Valerio D'Andrea ◽  
Antonio Panebianco ◽  
Alessandro Giuffrida
Keyword(s):  
Quality Assessment ◽  
Mean Value ◽  
Raw Material ◽  
Human Consumption ◽  
Salmonella Spp ◽  
The Mean ◽  
Fresh Products ◽  
Vibrio Spp ◽  
Zeus Faber

n the last few years, the consumption of fish eggs has increased rapidly, finding widespread use also in mass catering. This increase has involved also those of the Peter’s fish (Zeus faber). Females of this species, by their reproductive characteristics, have highly developed gonads in different periods of the year, making the raw material easy to find. The aim of the present study was to perform a quality assessment of Zeus faber ovaries regularly commercialized for human consumption. A total number of 34 samples, divided in fresh (11) and frozen (23), were processed for microbiological characterization, parasitological and histological evaluations. Fresh and frozen samples have significant (P<0.01) differences in total bacterial charge, with values of 4.75±0.5 Log CFU/g and 3.65±0.7 Log CFU/g respectively. The mean value of Enterobacteriaceae was 2.58±0.7 Log CFU/g in fresh products, while 52.17% (12) of frozen samples reported loads of <1 Log CFU/g. No Salmonella spp. and Listeria monocytogenes were found. Aeromonas spp. was detected in two frozen sample (with loads of 2.2 and <1 Log CFU/g) and in 5 fresh ovaries with value ranged from 1.70 to 3.48 Log CFU/g. Vibrio spp. was found in 4 (36.36%) and 3 (13.04%) of fresh and frozen samples respectively, with loads always <1 Log CFU/g. All 31 Vibrio strains isolated, were identified as Vibrio alginolyticus, and 61.29% (19) of them was positive for the ToxRS factor and 6.45% (2) for ToxR. The 47.06% (16) of total samples showed infestations by larvae of Anisakis Type 1 in the serous and inside the ovary. In this last case, histologically it was found to be free larvae. This study attested satisfactory hygiene conditions for Zeus faber ovaries currently marked for human consumption. The presence of potentially pathogenic strains of V. alginolyticus and Aeromonas spp., but above all the frequent infestation by Anisakis larvae, represent a potentially hazard for the consumer.

scholarly journals Determination of Salmonella spp., E. coli VTEC, Vibrio spp., and Norovirus GI-GII in Bivalve Molluscs Collected from Growing Natural Beds in Sardinia (Italy)

Foods ◽  
2017 ◽  
Vol 6 (10) ◽  
pp. 88 ◽  
Author(s):  
Marta Marceddu ◽  
Sonia Lamon ◽  
Simonetta Consolati ◽  
Sara Ciulli ◽  
Roberta Mazza ◽  
...  
Keyword(s):  
Bivalve Molluscs ◽  
Salmonella Spp ◽  
E Coli ◽  
Vibrio Spp

scholarly journals Análisis microbiológico de dos muestras de pescado comercializadas en Tunja, Boyacá. Estudio de caso

2019 ◽  
pp. 52-65
Author(s):  
Gabriela Sanabria Sánchez ◽  
Jhojan Camilo Chiquillo Pompeyo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Oncorhynchus Mykiss ◽  
Salmonella Spp ◽  
Vibrio Spp ◽  
Estudio De Caso

La producción pesquera en Colombia representa una actividad económica creciente. Entre las especies más consumidas, se encuentran la trucha arcoíris (Oncorhynchus mykiss) y el bagre (Brachyplatistoma sp.). El primero fue introducido al país a finales de la década de los 30, mientras que el bagre es una especie propia de Latinoamérica. El objetivo de la presente investigación fue determinar la calidad microbiológica de una trucha y un bagre comercializados en Tunja, y realizar una comparación microbiológica de las dos muestras. Para ello, se emplearon medios selectivos y diferenciales para el aislamiento de patógenos como coliformes, Salmonella spp., Vibrio spp. y Staphylococcus aureus coagulasa positiva. Los resultados evidenciaron la presencia de cepas de Salmonella spp., Escherichia coli, y Vibrio spp. Finalmente, se concluye que las muestras analizadas, pese a ser de diferente origen comercial y contar con condiciones de hábitat y alimentación completamente diferentes, no cumplen con los requisitos microbiológicos para ser comercializadas y podrían representar una potencial amenaza para la salud del consumidor.

scholarly journals Microbiological analysis and antibacterial activity of pear samples

2016 ◽  
Vol 5 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Habiba Afroz ◽  
Tasnia Ahmed ◽  
Md Aftab Uddin
Keyword(s):  
Fungal Growth ◽  
Bacterial Count ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Two Samples ◽  
And Staphylococcus Aureus ◽  
Klebsiella Spp ◽  
Vibrio Spp

Present study attempted to isolate and quantify the microorganisms from the pear samples collected within Dhaka metropolis. Out of the five samples studied, total viable bacterial count and the total coliform count were near about 105 cfu/g and 104 cfu/g, respectively. Among the specific pathogens, prevalence of Salmonella spp. and Vibrio spp. were found only in two samples. Whereas Klebsiella spp, Pseudomonas spp. and Staphylococcus aureus were detected in all the samples studied. Fungal growth was also observed in each sample within the range of 8.8×104 – 9.5×104 cfu/g. The study of antibiogram revealed that, most of the pathogens were resistant to more than one antibiotic. Interestingly the pear samples showed no anti-bacterial activity against any of the laboratory isolates such as, Escherichia coli, Pseudomonas spp., Salmonella spp. and Bacillus spp.Stamford Journal of Microbiology, Vol.5(1) 2015: 1-4

scholarly journals Assessment of Antibiotics and Fluoride Toothpastes against Pathogenic Bacteria associated with Dental Caries

2018 ◽  
pp. 1-10
Keyword(s):  
Dental Caries ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Significant Activity ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Test Organisms ◽  
Susceptibility Tests ◽  
Vibrio Spp

Dental caries is the most contagious disease throughout the world. In the present study, a total of 100 dental caries patients were selected and samples from these patients were collected through sterile pincers for microbiological examination. A total of 9 different pathogenic bacterial isolates were identified through conventional culturing technique and among them maximum number of occurrence was by Salmonella spp (26.13%), followed by E. coli (17.39%), Proteus spp (17.39%), Shigella spp (8.69%), Pseudomonas spp (8.69%), Vibrio spp (8.69%), Enterobacter spp (4.34%), Yersinia spp (4.34%) and S. mutans (4.34%). Furthermore, antibiotic susceptibility tests were performed by 9 different commercially available antibiotics i.e. vancomycin, erythromycin, clindamycin, amoxicillin, tetracycline, rifampicin, ampicillin, ticarcillin and metronidazole/silver sulphadizine. Kirby Bauer disc diffusion method was used against test organisms and it was observed that all these antibiotics expressed significant activity against greater part of test organisms but few of them showed resistance to metronidazole/silver sulphadizine. In addition to this, different toothpastes encoded as TP-1, TP-2, TP-3, TP-4 and TP-5 were used at a concentration of 20 mg/mL in order to evaluate their activity against test organisms and it was observed that all these toothpastes showed significant activity against isolated bacteria. It was concluded that all these toothpastes contained fluoride which supports to avoid tooth decay and promote minty fresh breath. Furthermore, it was recommended that avoid usage of too much sweets, chocolates, candies especially in children/adults, and there must be a proper use of toothpastes twice a day at every stage of life. Keywords: Dental Caries, Pathogenic Bacteria, Antibiotics, Fluoride Toothpastes

scholarly journals Detection of Vibrio spp., Salmonella spp., and Shigella spp. among the frozen food samples employing enrichment culture technique

2016 ◽  
Vol 5 (1) ◽  
pp. 26-29 ◽  
Author(s):  
Tahmina Shammi
Keyword(s):  
Enrichment Culture ◽  
Bacterial Load ◽  
False Negative ◽  
Food Samples ◽  
Food Preservation ◽  
Salmonella Spp ◽  
Potential Health ◽  
Frozen Food ◽  
Shigella Spp ◽  
Vibrio Spp

Freezing has long been an established method for food preservation. Freezing temperature may act as a stress factor for microbial cells, transforming the cells into injured or dormant state. Upon inoculation, these debilitated cells cannot grow on solid media and hence produce false negative results. Foods contaminated with injured cells of pathogenic bacterial strains are of potential health risk. Employing enrichment cultivation technique, present study attempted to detect such injured, dormant or viable but non culturable (VBNC) cells in different frozen food samples, collected from local markets and super-shops of Dhaka metropolis. Compared to the conventional cultivation means, the enrichment procedure revealed a significant increase in bacterial burden as well as increase in the pathogenic load. A maximum of 3 log increase in case of total bacterial load while 4 log, 5 log and 2 log increase in case of Vibrio spp., Salmonella spp. and Shigella spp., consecutively were observed. These findings clearly demonstrated the presence of injured cells in frozen foods which could be lethal under normal condition thereby posing public health risk.Stamford Journal of Microbiology, Vol.5(1) 2015: 26-29

scholarly journals Vibrio spp. and Salmonella spp., presence and susceptibility in crabs Ucides cordatus

2004 ◽  
Vol 46 (4) ◽  
pp. 179-182 ◽  
Author(s):  
Regine H.S.F. Vieira ◽  
Elenice Araújo de Lima ◽  
Dannielle Batista Rolim Sousa ◽  
Eliane Falavina dos Reis ◽  
Renata Garcia Costa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Nalidixic Acid ◽  
Enterobacter Cloacae ◽  
Species Level ◽  
Salmonella Spp ◽  
Ucides Cordatus ◽  
Number Of Individuals ◽  
Vibrio Spp

The presence of Vibrio spp. and Salmonella spp. in crabs marketed at the Bezerra de Menezes Ave., Fortaleza, State of Ceará, Brazil, was assessed between February and May, 2003. The number of individuals sampled in each one of the fifteen weekly samplings ranged between four and eight. Seven strains of Salmonella, from four different samplings, were identified, being five of them identified as serotype S. Senftenberg and two as S. Poona. All strains of Salmonella were sensitive to the tested anti-microbial drugs, with the exception of tetracycline and nalidixic acid, for which an intermediary sensibility was found. The MPN's for Vibrio ranged between 110/g and 110,000/g. Of the forty five Vibrio strains isolated from the crab samples, only 10 were identified up to the species level: two V. alginolyticus and eight V. parahaemolyticus. Bacteria belonging to the Enterobacteriaceae and Pseudomonaceae families were also identified, namely Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pantoea agglomerans and Pseudomonas aeruginosa. The proper cooking of the animals is recommended in order to avoid problems for the consumers of this crustacean.

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