Expression of OsPSTOL1 Improves the Phosphorus Utilization Efficiency of Transgenic Populus tomentosa
OsPSTOL1encodesa phosphorus (P) deficiency-tolerant protein expressed in the ausrice variety, Kasalash, which functions by enhancing the ability of the plant to obtain P and other nutrients; however, its role in woody plants remains unknown. In the present study, we isolated OsPSTOL1from Oryza sativa L. and subsequently generated OsPSTOL1-overexpressing transgenic Populus tomentosa. It was found that in the state of P deficiency, the transgenic P. tomentosahad a greater root length, the symptoms of P-deficiencyappeared much later, and the total nitrogen (TN) and phosphorus (TP) content was higher as compared with that of wild type plants. Upregulation of P-tolerance genes in transgenic P. tomentosaunder P-deficient conditions was analyzed by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR),the results of which were consistent with the physiological traits. In summary, these findings show thatOsPSTOL1 plays a positive role in the regulation of P uptake and utilization in transgenicP. tomentosatrees under P-deficient conditions and provide evidence for the potential application of OsPSTOL1in woody plants to overcome soil P deficiency.© 2021 Friends Science Publishers