Biological Activities of The Methanol Extracts of Smyrnium connatum Boiss. and Kotschy.

Berries, from the genusRubus, are among the vital components in a healthy diet. In this study, 80% methanol extracts from the three wildRubusspecies (Rubus moluccanusL.,Rubus fraxinifoliusPoir., andRubus alpestrisBlume) were evaluated for their phytochemical contents (total phenolics, flavonoid, anthocyanin, and carotenoid content), antioxidant (DPPH, FRAP, and ABTS assays), antiacetylcholinesterase, and antibacterial activities. GC-MS was used for quantification of naturally occurring phytochemicals. The results showed thatR. alpestriscontained the highest total phenolic [24.25±0.1 mg gallic acid equivalent (GAE)/g] and carotenoid content [21.86±0.63 mgβ-carotene equivalents (BC)/g], as well as the highest DPPH scavenging and FRAP activities. The highest total flavonoid [18.17±0.20 mg catechin equivalents (CE)/g] and anthocyanin content [36.96±0.39 mg cyanidin-3-glucoside equivalents (c-3-gE)/g] have been shown byR. moluccanus. For antibacterial assays,R. moluccanusandR. alpestrisextracts showed mild inhibition towardsBacillus subtilis,Staphylococcus aureus,Escherichia coli, andSalmonella enteritidis. Anticholinesterase activity for all extracts was in the range of 23–26%. The GC-MS analysis revealed the presence of at least 12, 21, and 7 different organic compounds in 80% methanol extracts ofR. alpestris,R. moluccanus, andR. fraxinifolius, respectively, which might contribute to the bioactivity.

Download Full-text

Phytochemical Constituency Profiling and Antimicrobial Activity Screening of Seaweeds Extracts Collected from the Bay of Bengal Sea Coasts

10.1101/680348 ◽

2019 ◽

Author(s):

Mahmudul Hasan ◽

Md. Abdus Shukur Imran ◽

Farhana Rumzum Bhuiyan ◽

Sheikh Rashel Ahmed ◽

Parsha Shanzana ◽

...

Keyword(s):

Antimicrobial Activity ◽

Biological Activities ◽

Inhibition Zone ◽

Antimicrobial Activities ◽

Total Phenolic ◽

Enteromorpha Intestinalis ◽

Bacterial Strains ◽

Hypnea Musciformis ◽

Methanol Extracts ◽

Seaweed Extracts

AbstractSeaweeds are able to produce a great variety of secondary metabolites that are characterized by a broad spectrum of biological activities. Two seaweeds species, namelyHypnea musciformisandEnteromorpha intestinalis werestudied to evaluate the phytochemical constituency and antimicrobial activities. First of all, crude extracts of both sea weeds were prepared by two different extraction methods (soaking and water bath) using different solvents. Phytochemicals profiling results revealed the presence of bioactive compounds (flavonoids, alkaloids, tannin, saponin and phenols) in both seaweed extracts. Quantification results for ethanolic extracts ofH. musciformis and E. intestinalisestimated 51 mg and 43 mg tannins in per gram of dried samples and flavonoids contents were found 67 mg and 57 mg/g mg QE/g respectively. Total phenolic contents were determined in terms of gallic acid equivalent (GAE).H. musciformisexhibited higher amount of phenolics (59 ± 0.0002 mg GAE/g) thanE. intestinalisextracts (41 ± 0.0002 mg GAE/g). In antimicrobial activity test, ethanol extractsof H. musciformisandE. intestinaliswere found 10 mm of inhibition diameter against all of the bacterial strains. Besides, methanol extracts ofE. intestinaliswere more susceptible toStaphylococcus aureus and Pseudomonaswhich was close to the inhibition diameter (>15 mm) of the mainstream antibiotic; Gentamicin. Moreover,Klebsiella sp. was found more susceptible to ethanol and methanol extracts of Hypnea musciformisas it showed inhibition zone greater than 15 mm. Both Seaweed extracts possessed higher amount of phytochemicals and showed promising antimicrobial activities when compared with the standards.

Download Full-text

Rapid Identification of Aldose Reductase Inhibitory Compounds fromPerilla frutescens

BioMed Research International ◽

10.1155/2013/679463 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 13

Author(s):

Ji Hun Paek ◽

Kuk Hyun Shin ◽

Young-Hee Kang ◽

Jae-Yong Lee ◽

Soon Sung Lim

Keyword(s):

Ethyl Acetate ◽

Chlorogenic Acid ◽

Aldose Reductase ◽

Rosmarinic Acid ◽

High Speed ◽

Soluble Fraction ◽

Biological Activities ◽

Methanol Extracts ◽

Chemical Structures ◽

Inhibitory Compounds

The ethyl acetate (EtOAc) soluble fraction of methanol extracts ofPerilla frutescens(P. frutescens) inhibits aldose reductase (AR), the key enzyme in the polyol pathway. Our investigation of inhibitory compounds from the EtOAc soluble fraction ofP. frutescenswas followed by identification of the inhibitory compounds by a combination of HPLC microfractionation and a 96-well enzyme assay. This allowed the biological activities to be efficiently matched with selected HPLC peaks. Structural analyses of the active compounds were performed by LC-MSn. The main AR inhibiting compounds were tentatively identified as chlorogenic acid and rosmarinic acid by LC-MSn. A two-step high speed counter current chromatography (HSCCC) isolation method was developed with a solvent system of n-hexane-ethyl acetate-methanol-water at 1.5 : 5 : 1 : 5, v/v and 3 : 7 : 5 : 5, v/v. The chemical structures of the isolated compounds were determined by1H- and13C-nuclear magnetic resonance spectrometry (NMR). The main compounds inhibiting AR in the EtOAc fraction of methanol extracts ofP. frutescenswere identified as chlorogenic acid (2) (IC50= 3.16 μM), rosmarinic acid (4) (IC50= 2.77 μM), luteolin (5) (IC50= 6.34 μM), and methyl rosmarinic acid (6) (IC50= 4.03 μM).

Download Full-text

LC-MS/MS Profiles and In Vitro Biological Activities of Extracts of an Endemic Species from Turkey: Stachys cretica ssp. anatolica

Plants ◽

10.3390/plants10061054 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1054

Author(s):

Ivana Carev ◽

Cengiz Sarikurkcu

Keyword(s):

Antioxidant Activity ◽

Biological Activity ◽

Biological Activities ◽

Antioxidant Properties ◽

Type Ii ◽

Endemic Plants ◽

Diabetes Mellitus Type Ii ◽

Methanol Extracts ◽

Promising Application

Background: Genus Stachys is one of the largest of the Lamiaceae family, having around 300 different plant species inhabiting areas with temperate and warm climates. The Stachys species in Turkey are represented with 81 taxa; 51 of them being endemic. Plants of the Stachys genus have been known for their biological activity and their use in ethnomedicine. Methods: The dominant components of S. cretica ssp. anatolica aqueous and methanol extracts were studied with the LC-MS/MS technique. Results: Chlorogenic acid, apigenin-7-glucoside and verbascoside present as the dominant polyphenols found in studied extracts. The prominent biological activity of the studied S. cretica ssp. anatolica methanol and aqueous extracts showed strong antioxidant activity and inhibition of enzymes tyrosinase and α-amylase, involved in skin disorders and diabetes mellitus type II. Conclusions: This study has proven that the aqueous and methanol extracts of S. cretica ssp. anatolica have prominent antioxidant activity, due to a high abundance of polyphenols. The strong antioxidant properties of S. cretica ssp. anatolica extracts show promising application for the pharmaceutical, food, and cosmetics industries.

Download Full-text

Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens Pleurosticta acetabulum and Cladonia subulata

Serbian Journal of Experimental and Clinical Research ◽

10.2478/sjecr-2020-0057 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Jovica Tomović ◽

Marijana Kosanić ◽

Branislav Ranković ◽

Perica Vasiljević ◽

Stevo Najman ◽

...

Keyword(s):

Cell Lines ◽

Biological Activities ◽

Reducing Power ◽

Natural Antioxidants ◽

Phytochemical Analysis ◽

Anticancer Activities ◽

Cytotoxic Effects ◽

Methanol Extracts ◽

Anticancer Effects ◽

Hela S3

Abstract The aim of this study is to investigate the chemical composition of methanol extracts of the lichens Pleurosticta acetabulum and Cladonia subulata and their antioxidant, and anticancer activities. The phytochemical analysis of the extracts of lichens was determined by HPLC-UV method. The predominant phenolic compounds in these extracts were norstictic acid and salazinic acids in P. acetabulum, while hypoprotocetraric acid and fumarprotocetraric acid were the major metabolites detected in C. subulata. Total phenolics and flavonoids in the extracts were determined spectrophotometrically, with the varied amount from 21.31 to 73.45 mg GA/g and from 8.48 to 15.42 mg RU/g, respectively. The lichen extracts showed comparable and strong antioxidant activity, exhibited higher DPPH and hydroxyl radical scavengings, inhibitory activity towards lipid peroxidation and reducing power. Cytotoxic effects of lichens were tested against HeLa S3 and LS174 cell lines using MTT method.The cytotoxic effects of P. acetabulum and C. subulata extracts toward two cancer cell lines were in the range from 39.17 to >200 μg/mL IC50 value. The present study showed that the tested extracts of lichens demonstrated important antioxidant and anticancer effects. That suggests that these lichens can be used as new sources of the natural antioxidants and anti-cancer compounds.

Download Full-text

Nutritional Composition and Biological Activities of the Methanol Extracts of Sea Mustard (Undaria pinnatifida) in Market.

Journal of Life Science ◽

10.5352/jls.2008.18.3.387 ◽

2008 ◽

Vol 18 (3) ◽

pp. 387-394 ◽

Cited By ~ 10

Author(s):

Jae-Suk Choi ◽

Hee-Jung Bae ◽

Yang-Chun Kim ◽

Nam-Hee Park ◽

Tae-Bong Kim ◽

...

Keyword(s):

Undaria Pinnatifida ◽

Biological Activities ◽

Nutritional Composition ◽

Methanol Extracts ◽

Sea Mustard

Download Full-text

Biological Activities of Extracts from Different Parts of Cryptomeria japonica

Natural Product Communications ◽

10.1177/1934578x1601100939 ◽

2016 ◽

Vol 11 (9) ◽

pp. 1934578X1601100 ◽

Cited By ~ 6

Author(s):

Hiroki Horiba ◽

Toshinori Nakagawa ◽

Qinchang Zhu ◽

Ahmed Ashour ◽

Atsushi Watanabe ◽

...

Keyword(s):

Cryptomeria Japonica ◽

Biological Activities ◽

Forest Tree ◽

In Vitro Assays ◽

Total Phenolic ◽

Melanin Biosynthesis ◽

Methanol Extracts ◽

Health Related ◽

Male Flowers

Cryptomeria japonica is one of the most important forest tree species in Japan. To increase the demand for domestic timber, broad uses related to the functional attributes of C. japonica must be developed. Several studies have examined the usefulness of C. japonica in terms of its biological activities, but a comprehensive study subjecting all parts of the C. japonica plant to the same solvent for extraction has not been done. Here, methanol extractions from the leaves, branches, bearing branches, male flowers, female flowers, cones, bark, heartwood, sapwood, pith, rhizomes, roots, and pollen were subjected to several in vitro assays of their biological activities such as antioxidant activity, anti-lipase activity, antibacterial activity and melanin-biosynthesis-inhibition activity. Their total phenolic content was also determined. The methanol extracts from each part of C. japonica except for pollen showed strong activities in the bioactivity assays. Furthermore, the methanol extracts were analyzed by GC/MS. The phytochemical profile varied among extracts from various parts of C. japonica. Our results suggest the great potential of C. japonica for use as a functional ingredient in health-related products.

Download Full-text

Antioxidant and antibacterial activity of Acorus calamus. L leaf and rhizome extracts

Jurnal Gizi Klinik Indonesia ◽

10.22146/ijcn.17937 ◽

2017 ◽

Vol 13 (4) ◽

pp. 144 ◽

Cited By ~ 1

Author(s):

Kho See Li ◽

Chan Sook Wah

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Biological Activities ◽

Radical Scavenging ◽

Diffusion Method ◽

Total Phenolic ◽

Acorus Calamus ◽

Methanol Extracts ◽

Antioxidant Agents ◽

Sweet Flag

Background: Acorus calamus (sweet flag) is a well-known traditional herb used in India. There were only limited studies done on both hydrophilic and hydrophobic extracts of A. calamus and the biological activities of phenolic and flavonoid compounds as well as alpha-asarone in the sweet flag.Objective: The present study was designed to explore the antioxidant and antibacterial activities of both hydrophilic and hydrophobic of A. calamus leaf and rhizome extracts. Lastly, the correlation between the biological activities and total phenolic content (TPC), total flavonoid content (TFC) as well as total alpha-asarone content (TAC) of three different extracts (water, hexane and methanol extracts) was revealed.Method: TAC was determined through using HPLC method, while TPC and TFC were determined through spectrophotometric method. DPPH-free radical scavenging assay (DPPH-FRS) and ferric reducing power assay (FRAP) were used to analyze antioxidant activity, whereby disc diffusion method was used to analyze the antibacterial activity.Results: Methanol extracts had significantly (p<0.05) highest levels of antioxidant activities, antibacterial activity TPC (4.466%1; 3.603%2) and TFC (12.819%1; 7.411%2) content. The significant (p<0.05) highest TAC was observed in hexane extracts (0.762%1; 2.975%2). TPC (p<0.05; r=0.855) and TFC (p<0.05; r=0.855) correlated to FRAP, while DPPH-FRS solely correlated to TFC (p<0.05; r=0.873).Conclusion: Both of the hydrophilic and hydrophobic extracts of A. calamus could be potent antioxidant agents for nutraceutical application and antibacterial agent for food application. Lastly, flavonoids and phenols extracted from sweet flag could be a good potent source of antioxidants for nutraceuticals or food additives as those compounds are correlated to the antioxidant activity.1 Leaf extract; 2Rhizome extract

Download Full-text