scholarly journals Chromatographic Fingerprint Analysis and Bioactivity of Origanum Vulgare Extracts

2016 ◽  
Vol 3 (2) ◽  
pp. 95-98
Author(s):  
Corina Bubueanu ◽  
Ioana Nicu ◽  
Lucia Pirvu

A very important measure for any country, in terms of public health problems and commercial exploitation, must be the development of medicinal and aromatic plant resources. Long-term strategies for the conservation of medicinal and aromatic plants, personnel training, cultivation or passage in culture of valuable species, sustainable methods of harvesting and processing plants to promote scientific research and validation of traditional remedies are needed. In the present, the natural compounds research are mainly focused on their role in improving health.  The extraction processes of the natural compound are significant both in scientific and economic terms. The goal is to obtain extracts with pharmacological activity with a minimum production cost. In this paper are analyzed hot and cold ethanol 50 % extracts of Origanum vulgare (Fam. Lamiaceae) species in terms of qualitative and quantitative chemical composition, and their antioxidant and antimicrobial potential. The results obtained showed no significant differences in terms of chemical composition and bioactivity, between the extracts. Thus, in industry sector these results can be used for reducing cost production.  

2009 ◽  
Vol 45 (No. 2) ◽  
pp. 66-73 ◽  
Author(s):  
M. Zouhar ◽  
O. Douda ◽  
D. Lhotský ◽  
R. Pavela

The aim of this study was to assess the nematicidal activity of different essential oils from medicinal and aromatic plants for use in nematode management. Essential oils of <I>Eugenia caryophyllata, Origanum compactum, Origanum vulgare, Thymus matschiana</I> and <I>Thymus vulgaris</I> showed nematicidal activity against <I>Ditylenchus dipsaci.</I>


Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 727 ◽  
Author(s):  
Antonios Chrysargyris ◽  
Maria Mikallou ◽  
Spyridon Petropoulos ◽  
Nikolaos Tzortzakis

In the present study, the yield, the chemical composition, and the antioxidant activities of the essential oils (EOs) of eight medicinal and aromatic plants (MAPs) cultivated under two environmental conditions characterized by a different altitude (namely mountainous and plain) were evaluated. Cultivation at different environmental conditions resulted in significant differences in the chemical composition and antioxidant activity for most of the studied species. In particular, high altitudes resulted in increased phenolic compounds’ content and antioxidant activity for artemisia plants, while specific parameters increased in the case of spearmint (total phenols) and rosemary (flavonoids). In contrast, in pelargonium, all the tested parameters were positively affected in the plain area, whereas, for laurel and sage, only flavanols remained unaffected. EO yield in mountainous pelargonium and spearmint decreased while, in mountainous laurel, pelargonium and spearmint increased when compared to plain areas. In addition, the major EO constituents’ content for most of the species were affected by environmental conditions. The 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and ferric reducing antioxidant power (FRAP) were variably correlated with total phenols, flavonoids, and flavanols, depending on the species and the altitude. Lastly, in limited cases, antioxidant activity (DPPH or FRAP values) was positively correlated with some EO components (e.g., borneol and β-pinene in artemisia and laurel plants grown in the plain, respectively, or 1,8-cineole in mountainous grown verbena plants). In conclusion, environmental conditions (altitude) affected antioxidants’ content and EO yield and composition of the studied MAPs. These findings can be used to introduce cultivation of MAPs in specific ecosystems for the production of high added value products.


2020 ◽  
Vol 16 (2) ◽  
pp. 106-111
Author(s):  
Ioannis Sarakatsianos ◽  
Konstantinos Adamopoulos ◽  
Victoria Samanidou ◽  
Athanasia Goula ◽  
Elissavet Ninou

Background: Medicinal plants consist a rich source of natural pharmacologically active compounds. The aim of the current work, was the study and optimization of the extraction conditions of phenolic constituents from important medicinal and aromatic plants, id. Sideritis raeseri, Sideritis scardica and Origanum vulgare, implementing microwave assisted extraction technique. Methods: Optimization was based on various parameters affecting extraction procedure, such as the duration of microwave radiation, the ratio of plant material mass to the solvent, the type and the concentration of the solvent. Results: The results revealed that 10 min of microwave radiation provided the maximum extraction yield of the phenolic constituents. The ratio of the plant material mass and the solvent had minor effect to the extraction efficiency. Conclusion: The examined solvents provided an extraction yield of phenolic constituents following the order: EtOH<MeOH<H2O<MeOH:H2O (80:20, v/v)<EtOH:H2O (60:40, v/v), in the case of Sideritis raeseri and Sideritis scardica and the order: H2O<MeOH:H2O (80:20, v/v)<EtOH:H2O (60:40, v/v) < MeOH<EtOH in the case of Origanum vulgare.


2017 ◽  
Vol 34 ◽  
pp. 128-134 ◽  
Author(s):  
R.R. Gyawali ◽  
H.R. Paudel

Jumla is one of the districts in high mountain region of Nepal from where the medicinal and aromatic plants, their parts and products are exported annually. A study was carried out from March to June 2014 to document the plant resources used in ethno veterinary practice by local people. Focal group discussions and key informant interviews were carried out to enlist plant bio resources used as ethno veterinary medicines. It was revealed that people used 42 plant resources to correct 28 different ailments in animals. Among the listed plant resources, about 87% were the home products of the district. Hence, the study led to the conclusion that there is opportunity to explore the locally found medicinal plants as ethno veterinary medical practice and commercial veterinary herbal medicine.


Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 475
Author(s):  
Beatriz H. Paschoalinotto ◽  
Maria Inês Dias ◽  
José Pinela ◽  
Tânia C.S.P. Pires ◽  
Maria José Alves ◽  
...  

The chemical composition and biological properties correlation in several medicinal and aromatic plants is still underexplored, especially in its most common form of consumption as tisane. The present study aims to characterize the organic acids and vitamin E composition of five tisanes and their extracts by High-Performance Liquid Chromatography coupled to a diode-array detector (HPLC-DAD) and HPLC coupled to a fluorescence detector techniques, respectively, and the phenolic composition by HPLC-DAD-ESI/MS (mass spectrometry by electrospray ionization). It also focuses on their bioactive properties, namely antioxidant, antimicrobial, anti-inflammatory, cytotoxic, anti-tyrosinase, and anti-diabetic activities. A Principal Component Analysis (PCA) was performed in order to understand the correlation between the chemical composition and bioactive properties of the tisanes. The tisane 5 (T5) composed by lemon thyme, tutsan, cloves, and cinnamon, was the most promising mixture, presenting the lowest values for the lipid peroxidation inhibition, anti-inflammatory, and anti-diabetic activity. It also presented the highest concentration of phenolic acids (caffeoylquinic acids derivatives), and flavan-3-ols (catechin derivatives). Only the dry plants presented tocopherols. For the antihemolytic, antimicrobial, and cytotoxic activity, T2 and T4 (with lemon thyme) were highlighted as the best herbal mixtures. The PCA proved to be a valid tool to select the most promising tisane according to the bioactivity. These results suggest that the studied tisanes can be source of high added-value bioactive compounds with health-promoting effects and potential for application in the food and nutraceutical industries, among others.


Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
NS Abdel-Azim ◽  
KA Shams ◽  
MM El-Missery ◽  
SI Ismail ◽  
FM hammouda

2013 ◽  
Vol 40 (1) ◽  
pp. 43-48
Author(s):  
Nina Ciocârlan

Abstract This work refers to the native species of genus Astragalus L. (A. dasyanthus, A. ponticus), Adonis L. (A. vernalis, A. wolgensis) and Digitalis L. (D. lanata, D. grandiflora). The plants are cultivated in the Botanical Garden of Moldova in the field collection of the medicinal and aromatic plants. Investigation includes propagation aspects, research into cultivation techniques and conservation measures. The biological particularities and the phenologic rhythm are also registered. The obtained data shows the ecological flexibility of species and the possibility of preserving them in culture.


2010 ◽  
Vol 9 (12) ◽  
pp. 1671-1679
Author(s):  
Oliviu Grigore Pop ◽  
Angela Marculescu ◽  
Romulus Gruia

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 192 ◽  
Author(s):  
Sanae Akkaoui ◽  
Anders Johansson ◽  
Maâmar Yagoubi ◽  
Dorte Haubek ◽  
Adnane El hamidi ◽  
...  

In this study, the essential oil of Origanum vulgare was evaluated for putative antibacterial activity against six clinical strains and five reference strains of Aggregatibacter actinomycetemcomitans, in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography–mass spectrometry coupled (CG–MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), p-cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the A. actinomycetemcomitans strains. O. vulgare essential oil did not interfere with the neutralizing capacity of Psidium guajava against the A. actinomycetemcomitans leukotoxin. In addition, it was shown that the O. vulgare EO had an antibacterial effect against A. actinomycetemcomitans on a similar level as some tested antimicrobials. In view of these findings, we suggest that O.vulgare EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to A. actinomycetemcomitans. In addition, it can be used together with the previously tested leukotoxin neutralizing Psidium guajava.


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