scholarly journals The Accumulation of Heavy Metals and Cytometric Characteristics Features of Red Blood Cells in Different Ages of Carp Fish from Zaporozhian Reservoir

2016 ◽  
Vol 53 ◽  
pp. 72-79 ◽  
Author(s):  
Olena Fedonenko ◽  
Nataliіa Yesipova ◽  
Tetyana Sharamok

The research was held in terms of Zaporozhian Reservoir (Ukraine) from 2014 till 2015 year. It was established that prussian carp (Carassius gibelio Bloch, 1782) and roach (Rutilus rutilus Linnaeus, 1758) underyearlings accumulate essential elements more extensively, especially zinc, comparing to adults ones. The species characteristic of heavy metals accumulation in the carp fish body was observed. The intensity level of erythropoiesis occurrence was higher in young fish of both species of carp fish. The specific features of cytometric characteristics of fish erythrocytes were identified: the relative amount of mature red blood cells prevailed in roach, and the area of mature red blood cells was significantly higher in prussian carp. In addition, in the young roach among immature forms of red blood cells significantly higher percentage of polychromatophil normoblasts was revealed.

Author(s):  
Raimondas Leopoldas Idzelis ◽  
Vytautas Kesminas ◽  
Gintaras Svecevičius ◽  
Vaidas Misius

The paper analyses the effects of heavy metals on freshwater fishes. From the point of view of pollution caused by heavy metals, the condition of ecosystems in Lithuania is yet less affected by heavy metals compared to that the world over. Fishes accumulate heavy metals selectively. Fishes mostly accumulate Pb, Cr, Cd. Our experiments showed that MPC (Maximum Permitted Concentration) (0.2 mg/kg) of lead was exceeded 1.6 time in the muscles of perch, and 1.4 time ‐ in the muscles of roach. The MPC (0.05 mg/kg) of cadmium was exceeded 1.2 time in the muscles of perch and roach. It was determined that perch accumulate heavy metals more intensively than roach. Heavy metals accumulate in fishes: liver > muscle> gills. As heavy metals are considered to be one of the most harmful water pollutants, there is a great need for a constant ecological monitoring and reduction of their amounts released into the environment. According to long‐term research data, the MPC in fishes is exceeded only slightly. This confirms a good condition of aquatic ecosystems in Lithuania. Santrauka Įvertintas sunkiųjų metalų pasiskirstymas gėlavandenių žuvų audiniuose monitoringiniuose vandens telkiniuose, taip pat ir kuojų bei ešerių, paveiktų sunkiųjų metalų didžiausiomis leidžiamosiomis koncentracijomis (DLK), audiniuose. Eksperimentiniai tyrimai atlikti su Cu, Zn, Ni, Cr, Pb, Cd, kurių kiekvienas pasižymi kancerogeniniu mutageniniu poveikiu. Nustatyta, kad švino DLK (0,2 mg/kg) buvo viršyta ešerių raumenyse 1,6 karto, o kuojų – 1,4 karto. Kadmio DLK (0,05 mg/kg) ešerių ir kuojų raumenyse buvo viršyta apie 1,2 karto. Taip pat nustatyta, kad ešeriai sunkiuosius metalus kaupia intensyviau nei kuojos. Eksperimentiniai duomenys sutampa su sunkiųjų metalų tyrimų monitoringiniuose vandens telkiniuose duomenimis. Žuvų audiniuose Pb ir Cd koncentracijos yra didžiausios ir dažnai viršija Lietuvos higienos normoje nurodytąją DLK. Tokie rezultatai įpareigoja nuolat kontroliuoti sunkiųjų metalų kiekį žuvyse. Rezultatai pateikiami sunkiųjų metalų sklaidos pasiskirstymo grafikuose. Резюме Представлены данные по распределению тяжелых металлов в тканях пресноводных рыб в мониторинговых водоемах, а также результаты, полученные путем воздействия тяжелых металлов в предельно допустимых концентрациях (ПДК) на окуня и плотву. В экспериментальных исследованиях применялись металлы Cu, Zn, Ni, Cr, Pb, Cd, оказывающие на живые организмы воздействие мутагенного и канцерогенного характера. Установлено, что концентрация Cd в тканях окуня превышала ПДК (0,2 мг/кг) в 1,6 раза, а в тканях плотвы – в 1,4 раза. Концентрация Cd в тканях тех же рыб превышала ПДК (0,05 мг/кг) примерно в 1,2 раза. Также установлено, что окунь аккумулирует тяжелые металлы интенсивнее плотвы. Экспериментальные данные совпадают с данными, полученными в мониторинговых водоемах. Установлено также, что концентрация Pb и Cd в тканях рыб зачастую превышает ПДК. Полученные результаты обязывают постоянно контролировать уровень тяжелых металлов в тканях рыб.


Author(s):  
Kais Magomedovich GUSEYNOV ◽  
Ruslan Magomedovich BARKHALOV ◽  
Aysha Sharapatinovna GASANOVA ◽  
Marina Vladimirovna KHLOPKOVA ◽  
Umshanat Dzhamaldinovna ZURKHAEVA

The article presents the results of studies of feeding characteristics of such cyprinid fish species as the roach Rutilus rutilus caspius (Jakowlev, 1870) and the bream Abramis brama (Linnaeus, 1758) in various parts of the Daghestan coast of the Caspian Sea. The material was sampled along the entire Daghestan coast of the Caspian Sea, from the Kizlyar Bay to the mouth of the Samur river, in different biological seasons in the period of 2017–2018.


2020 ◽  
Vol 1 ◽  
pp. 92-96
Author(s):  
L. Yu. Karpenko ◽  
◽  
P. A. Polistovskaya ◽  
A. I. Enukashvili ◽  
A. B. Balykina ◽  
...  

The article is devoted to the assessment of the toxic effects of cadmium acetate on fish. The research topic is relevant, since heavy metals, and especially cadmium, are ones of the most dangerous pollutants for the aquatic environment. After the emission of the already used water by the electricity-power plants into the reservoirs of circulating wa-ter, a sharp increase in the maximum permit-ted concentrations of heavy substances can be observed often, which act on hydrobi-onts for a rather short period of time. That is why, modeling this effect is necessary to study the effects of heavy metals. The aim of the assay was to study the hematological parameters of carp after exposure to various concentrations of cadmium acetate. During the experiment, 5 groups of fish were formed - 1 control group (10 fish), 4 experimental groups - 10 fish each. The experimental groups of fish were exposed in a solution of cadmium acetate (Cd (CH3COO) 2) with concentrations of 0.05 mg / l, 0.5 mg / l, 5 mg / l, 50 mg / l (exceeding the maximum permissible concentration of cadmium for fisheries in 10, 100, 1000 and 10000 times respectively). We have investigated: the erythrocyte sedimentation rate, the number of red blood cells in the blood and the con-centration of hemoglobin in the blood. Dur-ing the study, the authors noted an increase in the erythrocyte sedimentation rate due to the formation of conglomerates of blood cells with plasma proteins, as well as a de-crease in the number of red blood cells due to the toxic effect of cadmium acetate. A decrease in the concentration of hemoglobin in the blood was also observed, probably associated with a decrease in the number of red blood cells due to their lysis. With an increase in the concentration of the metal under study, there is a significant increase in ESR, a decrease in the level of hemoglobin and the number of red blood cells.


Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.


Author(s):  
John A. Trotter

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).


Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.


Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).


2001 ◽  
Vol 120 (5) ◽  
pp. A356-A357
Author(s):  
M FURUKAWA ◽  
Y MAGAMI ◽  
D NAKAYAMA ◽  
F MORIYASU ◽  
J PARK ◽  
...  

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