scholarly journals Prevalence, antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and Klebsiella oxytoca among hospitalized patients

2016 ◽  
Vol 118 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Sourav Chakraborty
Keyword(s):  
Klebsiella Pneumoniae ◽  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Hospitalized Patients ◽  
Esbl Production ◽  
Susceptibility Profiles

scholarly journals Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

2019 ◽  
Vol 79 (4) ◽  
pp. 555-565 ◽  
Author(s):  
A. F. G. Rave ◽  
A. V. Kuss ◽  
G. H. S. Peil ◽  
S. R. Ladeira ◽  
J. P. V. Villarreal ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Effluent Treatment ◽  
Automated System ◽  
Resistant Bacteria ◽  
Biochemical Tests ◽  
Biochemical Identification ◽  
Identification Techniques ◽  
Susceptibility Profiles ◽  
The Impact

Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of the Universidade Federal de Pelotas, Pelotas, Brazil. Bacteria were identified by conventional biochemical tests and by the VITEK ®2 automated system. Further, the susceptibility profile to antibiotics was also determined by the automated system. Six species of bacteria (Raoutella planticola, K. pneumoniae ssp. pneumoniae , Serratia marcescens, Raoutella sp., E. cloacae and Klebsiella oxytoca) were identified by conventional biochemical tests, while three species of bacteria (K. pneumoniae ssp. pneumoniae, S. marcescens and K. oxytoca ) were identified by VITEK®2 automated system. VITEK ®2 indicated agreement in 19 (79.17%) isolates and difference in five (20.83%) isolates when compared to results from conventional biochemical tests. Further, antibiotic susceptibility profile results showed that all isolates (100%) were resistant to at least one out of the 18 antibiotics tested by VITEK®2. Thus, no multi-resistant bacteria that may be used in effluent treatment systems or in bioremediation processes have been reported. Results indicate VITEK ® 2 automated system as a potential methodology in the determination of susceptibility profile and identification of environmental bacteria.

scholarly journals Presence of carbepenemase-producing Enterobacteriaceae in the River Lambro basin, Italy: might sediment represent an important resistance reservoir?

2021 ◽  
Author(s):  
Sara Giordana Rimoldi ◽  
Francesca Romeri ◽  
Anna Gigantiello ◽  
Cristina Pagani ◽  
Luigi Viganò ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Human Health ◽  
Klebsiella Oxytoca ◽  
River Sediments ◽  
Rapid Spread ◽  
Susceptibility Profiles ◽  
Living Bacteria

In the last years, the rapid spread in anthropized ecosystems of pathogens which are resistant to carbapenem antibiotics has raised great concern. In this study, KPC-producing Klebsiella pneumoniae was found in the River Lambro in June 2019, whereas KPC-producing Klebsiella oxytoca and Citrobacter braakii were identified in untreated wastewaters. Susceptibility profiles indicated resistance to imipenem, ertapenem and meropenem. Different carbapenamase genes (blaKPC, blaNDM, blaVIM, blaOXA-48) were also found in the River Lambro, although not associated to living bacteria. The presence of a wide set of carbapenemase genes and resistant pathogens show that river sediments could act as a reservoir of antibiotic resistance potentially threatening human health.

scholarly journals Characterization of Klebsiella pneumoniae Strains Isolated from Urinary Tract Infection: Detection of ESBL Characteristics, Antibiotic Susceptibility and RAPD Genotyping

2013 ◽  
Vol 62 (4) ◽  
pp. 401-409 ◽  
Author(s):  
MUSTAFA ONUR ALADAG ◽  
AHMET UYSAL ◽  
NIYAZI DUNDAR ◽  
YUSUF DURAK ◽  
ERDOGAN GUNES
Keyword(s):  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Antibiotic Susceptibility ◽  
Test Method ◽  
Penicillin G ◽  
Diffusion Method ◽  
Esbl Production ◽  
Rapd Pcr ◽  
Pcr Method ◽  
Tract Infections

In this study, a hundred Klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (ESBL) production. The random amplified polymorphic DNA (RAPD-PCR) method was used to identify the genetic differentiation of K. pneumoniae isolates. A total of 26 different DNA bands ranging between 334 bp and 28033 bp were detected among the strains. It was found that 100 K. pneumoniae strains revealed 11 different RAPD profiles. Antibiotic susceptibility tests were conducted using a disc diffusion method against 16 antibiotics. Fifty-five different resistance profiles were determined among the strains. ESBL-productions of the strains were determined by the double disc synergy test (DDST) and ESBL E-test methods. ESBL production rates among the strains were found to be 55% by E-test method and 45% by DDST method. While ESBL-producing K. pneumoniae strains showed the greatest resistance to penicillin G (100%), followed by piperacillin (92.7%) and erythromycin (85.4%),the resistance rates of non ESBLproducing strains to those antibiotics were determined as 97.8%, 88.8% and 88.8%, respectively. Both groups of strains showed the highest sensitivity to meropenem. Based on the results obtained from the study, it was concluded that the detection of ESBL-producing strains by the E-test method was more sensitive than by the DDST method. Phenotypic and genotypic identification methods should be used together to detect ESBL presence. The RAPD-PCR method alone will not be adequate in the genotyping of the strains and alternative DNA-based methods should be used.

scholarly journals Nosocomial infection and characterization of extended-spectrum β-lactamases-producing Enterobacteriaceae in Northeast Brazil

2011 ◽  
Vol 44 (4) ◽  
pp. 441-446 ◽  
Author(s):  
Afonso Gomes Abreu ◽  
Sirlei Garcia Marques ◽  
Valério Monteiro-Neto ◽  
Roberto Morais Luz de Carvalho ◽  
Azizedite Guedes Gonçalves
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Klebsiella Oxytoca ◽  
Detection Methods ◽  
Northeast Brazil ◽  
Esbl Production ◽  
Extended Spectrum ◽  
Disk Method ◽  
Esbl Producers

INTRODUCTION: Extended spectrum β-lactamases (ESBLs) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. METHODS: During 2009, 659 enterobacteria strains were isolated from different clinical specimens and tested for ESBL production. The disk approximation test, combined disk method and addition of clavulanic acid were used for phenotypic detection of the ESBL-producing strains and PCR for detection of the blaTEM and blaCTX-M genes. RESULTS: Among the isolates, 125 were ESBL producers. The blaCTX-M and blaTEM genes were detected in 90.4% and 75% of the strains, respectively. Most strains were isolated from urine. Klebsiella pneumoniae was the most prevalent organism. Microorganisms presented high resistance to the antibiotics. CONCLUSIONS: These results support the need for extending ESBL detection methods to different pathogens of the Enterobacteriaceae family because these methods are only currently standardized by the CLSI for Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and Proteus mirabilis. Carbapenems were the antibiotic class of choice for the treatment of infections caused by ESBL-producing Enterobacteriaceae.

Klebsiella, Salmonella, E. coli

2011 ◽  
pp. 180-183
Author(s):  
Dr Mark Harrison
Keyword(s):  
Escherichia Coli ◽  
Critical Care ◽  
Klebsiella Pneumoniae ◽  
Klebsiella Oxytoca ◽  
Hospitalized Patients ◽  
Nosocomial Pathogen ◽  
Gi Tract ◽  
Gram Negative ◽  
E Coli

6.1 Klebsiella, 180 6.2 Salmonella, 181 6.3 Escherichia coli, 182 • Klebsiella pneumoniae and Klebsiella oxytoca • Enterobacteria • Gram negative rods • Encapsulated, produce exotoxins • Primarily opportunistic and nosocomial pathogen. • Colonizes skin, nasopharynx, and GI tract. • Frequently colonizes hospitalized patients and contaminant in critical care areas and ventilators....

Антимікробна резистентність провідних збудників інфекцій сечових шляхів у Києві (Україна)

2017 ◽  
Vol 1 (2) ◽  
pp. 48-60
Author(s):  
A.G. Salmanov ◽  
A.V. Rudenko
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
Klebsiella Oxytoca ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Providencia Rettgeri ◽  
E Coli

Мета роботи — вивчити резистентність до антибіотиків бактеріальних збудників інфекцій сечових шляхів (ІСШ), виділених у пацієнтів урологічного стаціонару в м. Києві. Матеріали і методи. Досліджено 1612 штамів бактерій, виділених із сечі хворих з ІСШ (цистит, уретрит, пієлонефрит), госпіталізованих в урологічне відділення ДУ «Інститут урології НАМН України» у м. Києві протягом 2016 р. Серед пацієнтів переважали жінки — 1201 (74,5 %). Вік хворих становив від 17 до 74 років. Для збору даних використано медичну документацію лікарні. Мікробіологічні дослідження виконано у лабораторії мікробіології ДУ «Інститут урології НАМН України». Аналізували результати культурального дослідження зразків сечі, зібраних за наявності клінічних ознак ІСШ. Дослідження клінічного матеріалу та інтерпретацію отриманих результатів проводили загальноприйнятими методами. Вивчено чутливість уропатогенів до 31 антибіотика дискодифузійним методом відповідно до рекомендацій Інституту клінічних та лабораторних стандартів США (Clinical and Laboratory Standards Institute (CLSI)). Результати та обговорення. Аналіз мікробного спектра сечі виявив домінування серед уропатогенів штамів Escherichia coli (32,0 %), Enterococcus faecalis (19,5 %), Klebsiella pneumoniae (10,9 %), Staphylococcus epidermidis (8,9 %), S. haemolyticus (6,5 %) та Pseudomonas aeruginosa (6,4 %). Частка Enterococcus faecium, Enterobacter aerogenes і Streptococcus viridans становила відповідно 2,5, 2,2 і 1,6 %, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter baumannii, Proteus vulgaris та Providencia rettgeri — менше 1,0 %. У більшості випадків (69,7 %) мікроорганізми виділено у монокультурі, у решті випадків — у мікробних асоціа- ціях. Високу резистентність до тестованих антибіотиків виявили штами E. aerogenes (45,1 %), E. cloacae (45,7 %), E. faecium (40,9 %), E. faecalis (40,7 %), E. coli (39,9 %), P. aeruginosa (34,0 %), K. pneumoniae (28,6 %). Найбільш активними до уропатогенів були іміпенем (E. coli — 87,6 %, P. aeruginosa — 75,7 %, E. cloacae — 67,3 %, E. aerogenes — 72,6 %, K. pneumoniae — 93,2 %), меропенем (E. coli — 89,1 %, P. aeruginosa — 76,7 %, K. pneumoniae — 82,6 %), лефлоцин (E. coli — 74,5 %, ентерококи — 78,7 %, P. aeruginosa — 76,7 %, E. cloacae — 73,9 %, E. aerogenes — 80,4 %, K. pneumoniae — 83,5 %), амоксицилін/клавуланат (ентерококи — 84,6 %), фурагін (ентерококи — 82,6 %), цефоперазон (K. pneumoniae — 89,2 %, P. aeruginosa — 73,8 %), цефтріаксон (K. pneumoniae — 80,1 %). Висновки. Антибіотикорезистентність збудників ІСШ — важлива терапевтична проблема. Найбільшою активністю до уропатогенів характеризуються іміпенем, меропенем, лефлоцин, амоксицилін/ клавуланат, фурагін, цефоперазон, цефтріаксон, які можна розглядати як препарат вибору для призначення стартової терапії ІСШ. Необхідно здійснювати постійний моніторинг за резистентністю до дії антибіотиків. Політику використання антибіотиків у кожному стаціонарі слід визначати залежно від локальних даних щодо резистентності до протимікробних препаратів.

Asociación en un mismo plásmido de blaVIM-1 y qnrS2 en Klebsiella pneumoniae y Klebsiella oxytoca aisladas en Sevilla

2012 ◽  
Vol 30 (5) ◽  
pp. 246-248
Author(s):  
José M. Rodríguez-Martínez ◽  
M. Carmen Conejo ◽  
Paula Díaz de Alba ◽  
Lorena López-Cerero ◽  
Pedro Fernandez-Echauri ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Klebsiella Oxytoca

scholarly journals Carriage ofStaphylococcus aureusamong 104 healthy persons during a 19-month period

1995 ◽  
Vol 115 (1) ◽  
pp. 51-60 ◽  
Author(s):  
N. H.Riewerts Eriksen ◽  
F. Espersen ◽  
V.Thamdrup Rosdahl ◽  
K. Jensen
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Statistical Difference ◽  
Phage Type ◽  
Hospitalized Patients ◽  
Index Number ◽  
Carrier Rate ◽  
Type Distribution ◽  
Nasal Carrier ◽  
Healthy Persons

SummaryThe present study was undertaken to investigate the frequency of the nasal carrier rate ofStaphylococcus aureus. The investigation was performed on 104 healthy persons. The total number of swabs performed was 1498 and this resulted in isolation of 522S. aureusstrains. All strains have been identified, tested for antibiotic susceptibility, and phage-typed. The carrier-index (number of positive swabs/number of total swabs for each individual person) was compared with different sampling and culturing methods, phage type, age, and resistance to antibiotics. There was statistical difference in carrier rate according to sex (P·05). Among the 104 persons 15 (14·4%) were persistent carriers, 17 (16·3%) intermittent carriers, 55 (52·9%) occasional carriers and 17 (16·3%) non-carriers. Among intermittent and occasional carriers the phage-type distribution was different from theS. aureusstrains isolated from Danish hospitalized patients in 1992, while the persistent carriers had similar phage-type distribution.

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