scholarly journals Comparison of hepatoprotective effect of aqueous and ethanolic extracts of Berberis lycium Royale (Sumbloo) in isoniazid induced hepatotoxicity in male mice model

2020 ◽  
Vol 9 (6) ◽  
pp. 835
Author(s):  
Saima Rafique ◽  
Khalida Ajmal ◽  
Uzma Naeem ◽  
Akbar Waheed ◽  
Ayesha Afzal ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Hepatoprotective Effect ◽  
High Dose ◽  
Dependent Manner ◽  
Male Mice ◽  
Mice Model ◽  
Ethanolic Extracts ◽  
Berberis Lycium

Background: The objective of the study was to compare the hepatoprotective effect of aqueous and ethanolic extracts of stem bark of Berberis lycium Royle in isoniazid (INH) induced hepatotoxicity in mice model.Methods: This randomized controlled in-vivo study conducted in male mice model from 10th April 2014 till 10th May 2014 at National Institute of Health, Islamabad. Group (C) was given normal diet and water. Group (D) was given isoniazid (50 mg/kg BW) to produce hepatotoxicity. Group (LA) and (HA) were given isoniazid (INH) plus low and high dose of aqueous extract of stem bark of Berberis lycium Royle respectively. Group (L.E) and (H.E) were given INH plus low and high dose of ethanolic extract of stem bark of Berberis lycium Royle respectively.Results: Hepatotoxicity produced by INH was shown by raised serum liver function tests (LFT’s), marked hepatocytic ballooning, significant steatosis and inflammation. Mice receiving simultaneous treatment of INH, low and high dose of aqueous extract of Berberis lycium Royle showed decrease serum LFT’s and their liver sections showed improved histological picture but more significant reduction in hepatotoxic effects were observed in animals receiving low and high doses of ethanolic extract.Conclusions: Hepatotoxicity of INH can be more fully reversed by simultaneous use of INH with ethanolic extract as it has better hepatoprotective potential in dose dependent manner as compare to aqueous extract of stem bark of Berberis lycium Royle.

scholarly journals Antioxidant Activity and Cytotoxicity of Taraxacum Hispanicum Aqueous and Ethanolic Extracts on Hepg2 Cells

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Gupta Meenu ◽  
Nogueira A ◽  
Almeida R ◽  
Oliveira A. I. ◽  
Oliveira R. F. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Viability ◽  
Hepg2 Cell ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
In Vitro Test ◽  
Dependent Manner ◽  
Chelating Activity ◽  
Ethanolic Extracts

Introduction: Plants belonging to the genus Taraxacum have been used in traditional medicine. Nowadays, extracts of these plants have been reported for the treatment of diseases, including liver disorders. Increasing interest and research on these plants also revealed its potential for treating cancer. This study aims to evaluate the antioxidant activity and cytotoxic properties of crude extracts from aerial parts of Taraxacum hispanicum H.Lindb, against human hepatocarcinoma (HepG2). Material and methods: Evaluation of the antioxidant properties was performed using DPPH in vitro test, superoxide scavenging assay and Fe2+ chelating activity. MTT assay was used to determine metabolic activity, for 24 and 48 hours. Results: For antioxidant capacity of the ethanolic extract (overall the one with the best results), IC50 values were 62.4 ± 6.7 μg/ml (DPPH radical scavenging activity) 53.9 ± 10.3 μg/ml (Fe2+ chelating activity) and 2.0 ± 0.3 μg/ml (superoxide scavenging assay). The aqueous and ethanolic extracts had different effects on HepG2 cell viability. Aqueous extract induced cell cytotoxicity in a time and dose-dependent manner, leaving only 52.6% viable cells at a concentration of 200 μg/ml, after 48 h. An increase in the cell viability was seen in the ethanolic extract, from 24 h to 48 h at higher concentrations. Conclusions: Ethanolic extract of T. hispanicum was the most promising, presenting anti-oxidative capacity and only the aqueous extract of the plant presented more relevant cytotoxicity over HepG2 cell line. These activities may be related with the extract phenolic content. However, further studies are needed to elucidate the main mechanisms responsible for these potential effects.

scholarly journals Isoniazid induced hepatotoxicity and its amelioration with ethanolic extract of stem bark of Berberis lycium Royale in mice

2017 ◽  
Vol 6 (8) ◽  
pp. 1865 ◽  
Author(s):  
Saima Rafiq ◽  
Khalida Ajmal ◽  
Ayesha Afzal
Keyword(s):  
Elevated Serum ◽  
Ethanolic Extract ◽  
Serum Levels ◽  
Stem Bark ◽  
Hepatoprotective Activity ◽  
Normal Diet ◽  
High Dose ◽  
Animal House ◽  
Berberis Lycium

Background: To study the hepatoprotective effect of Ethanolic extract of Stem Bark of Berberis lycium Royale in isoniazid (INH) induced hepatotoxicity in mice model.Methods: The study design was lab based randomized controlled in-vivo study in mice conducted from 9th April 2014 till 9th May 2014 at animal house of National Institute of Health, Islamabad. Group A was on normal diet and water and hepatotoxicity was produced by giving isoniazid (50mg/kg BW) in mice of Group B. Group C and D were given isoniazid (INH) plus low dose and high dose of Ethanolic extract of stem bark of Berberis Lycium Royle respectively.Results: INH induced hepatotoxicity was depicted by elevated serum LFT’s, hepatocytic ballooning, severe steatosis and inflammation. Mice getting concurrent treatment of INH, low and high dose of Ethanolic extract of Berberis Lycium Royle showed decreased serum levels of biomarkers and their liver sections manifested improved histological picture but more significant reduction in toxic effects were observed in animals receiving high dose.Conclusions: High dose of Ethanolic extract of stem bark of Berberis lycium Royale showed more marked hepatoprotective activity as compare to low doses. The hepatotoxicity of INH can be reduced by concurrent use of INH with ethanolic extracts of Berberis Lycium Royle.

scholarly journals Investigation of Anticancer Activity of Lindernia crustacea (L.) F. Muell. var. Crustacean

2018 ◽  
Vol 16 (5) ◽  
pp. 307-317
Author(s):  
Sahapat BARUSRUX ◽  
Natthida WEERAPREEYAKUL ◽  
Preeyaporn Plaimee PHIBOONCHAIYANAN ◽  
Munthipha KHAMPHIO ◽  
Waraporn TANTHANUCH ◽  
...  
Keyword(s):  
Cell Death ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Aqueous Extract ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Cancer Cell Lines ◽  
Dependent Manner ◽  
Ethanolic Extracts

Lindernia crustacea (L.) F. Muell. var. crustacean or “Ya Kap Hoi: YKH” was misunderstood as “Ya Yad Nam Kang” and cancer curative. This study aimed to investigate anticancer activity by the analysis of chemical constituent in extracts and in vitro screening of biological activities of the extract in several aspects such as cancer cell lines cytotoxic activity, immune cell proliferating activity, reducing power activity, alkylation activity. The HPLC analysis showed the absence of plumbagin peak observed in the HPLC chromatograms of both YKH aqueous and YKH ethanolic extracts. The YKH ethanolic extract yielded more chemical constituents than that of the YKH aqueous extract. The YKH aqueous extract was inactive against all cancer cells tested. Interestingly, YKH ethanolic extract caused cancer cell death in HCT116 colon cancer, HepG2 liver cancer, and Jurkat leukemic cancer cell lines in the concentration dependent manner. The following IC50 concentrations of the YKH ethanolic extract that possessed 50 % cell death after 24 h exposure in HCT116, HepG2, and Jurkat cell line were 195.4 ± 12, 171.7 ± 8.7, and 48.8 ± 5.7 µg/mL, respectively. Both aqueous and ethanolic extracts of YKH showed high antioxidant activity based on reducing power activity but did not have alkylation activity. At high concentration (250 µg/mL), YKH ethanolic extract can inhibit immune cells proliferation activity more than the YKH aqueous extract. An unexpected but critical outcome of our studies was the finding that anticancer activity is promised by selecting the plant extraction solvent with less polarity. Potential anticancer constituents were extracted from YKH using ethanol and these constituents cannot be found in aqueous solution of YKH.

The effect of deer antler from East Kalimantan to increase trabecular bone density and calcium levels in serum on osteoporotic mice

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Retno Widyowati ◽  
Suciati Suciati ◽  
Dewi Melani Haryadi ◽  
Hsin-I Chang ◽  
IPG Ngurah Suryawan ◽  
...  
Keyword(s):  
Bone Density ◽  
Trabecular Bone ◽  
Aqueous Extract ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Trabecular Bone Density ◽  
Male Mice ◽  
Deer Antler ◽  
East Kalimantan ◽  
Dose Dependent

Abstract Objectives Glucocorticoid-induced osteoporosis (dexamethasone) is a primary cause of secondary osteoporosis by the decreasing formation and increasing resorption activities. Previously, the in vitro study showed that 70% ethanol and aqueous extract of deer antler have increased alkaline phosphatase in osteoblast cell that known as marker of bone formation. The mind of this study is to analyze the effect of deer antlers in increasing the bone trabecular density of osteoporosis-induced male mice. Methods This study used a post-test control group design. A total of 54 healthy male mice were randomly divided to nine groups, i.e., healthy control, osteoporotic, positive control, 70% ethanol (4, 8, and 12 mg/kg BW), and aqueous extracts (4, 8, and 12 mg/kg BW) of deer antler groups. All of the interventions were given 1 mL of test sample for 4 weeks orally. The bone densities were determined using histomorphometry by Image J and Adobe Photoshop. The statistical data were performed using SPSS 23 and statistical significance was set at p<0.05. Results The results showed that alendronate group, 70% ethanol, and aqueous extract groups increased bone density and calcium levels in serum (p<0.05) compared to osteoporotic group in dose dependent manner. It indicated that 70% ethanol and aqueous extract of deer antler stimulating bone turnover and aqueous extract showed the highest. Conclusions Dexamethasone induction for 4 weeks caused osteoporotic mice and the administration of 70% ethanol and aqueous extracts of deer antler from East Kalimantan increased trabecular bone density and calcium levels in dose dependent manner.

scholarly journals HEPATOPROTECTIVE EVALUATION OF EPALTES DIVARICATA (L.) CASS. WHOLE PLANT EXTRACTS AGAINST PARACETAMOL-INDUCED HEPATOTOXICITY IN RATS

2016 ◽  
Vol 8 (12) ◽  
pp. 231 ◽  
Author(s):  
K. Amala ◽  
R. Ilavarasan ◽  
R. Arunadevi ◽  
S. Amerjothy
Keyword(s):  
Aqueous Extract ◽  
Histopathological Examination ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Dependent Manner ◽  
Traditional Use ◽  
Whole Plant ◽  
Medicinal Use ◽  
Histopathological Studies ◽  
Dose Dependent

<p><strong>Objective: </strong>The plant of <strong><em>Epaltes</em></strong><strong> <em>divaricata </em>(L.) </strong>Cass.<strong> Traditionally used for jaundice. </strong>The present work aimed to investigate the hepatoprotective activity of alcohol and aqueous extract of the whole plant against paracetamol-induced hepatotoxicity in rats to substantiate its traditional use.</p><p><strong>Methods: </strong>The alcohol and aqueous (200 and 400 mg/kg) extract of <em>Epaltes divaricata</em> prepared by cold maceration were administered orally to the animals with hepatotoxicity induced by paracetamol (1000 mg/kg). Silymarine (40 mg/k) was given as reference standard. Hepatoprotective activity was assessed by estimating marker enzymes and by histopathological studies.</p><p><strong>Results: </strong>Both alcohol and aqueous (200 and 400 mg/kg) extract treatment significantly restored the paracetamol-induced elevations in levels of serum enzymes aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphate (ALP) and total bilirubin in a dose-dependent manner. Histopathological examination revealed that the treatment attenuated the paracetamol-induced damage to the liver. The hepatoprotective effect of both extracts was comparable to that of the standard hepatoprotective agent, silymarin.</p><p><strong>Conclusion: </strong>The alcohol and aqueous extract of <em>E. divaricata</em> exhibited hepatoprotective effect against paracetamol-induced liver damage in rats. This study also validated their traditional medicinal use in jaundice.</p>

scholarly journals Evaluation of anticoagulant activity of aqueous extract of Cestrum nocturnum

2017 ◽  
Vol 8 (4) ◽  
pp. 525
Author(s):  
Chandra Kishore Tyagi ◽  
Deenanath Jhade ◽  
Sunil Kumar Shah
Keyword(s):  
Aqueous Extract ◽  
Anticoagulant Activity ◽  
Ethanolic Extract ◽  
Dependent Manner ◽  
Thrombin Time ◽  
Clotting Time ◽  
Standard Procedures ◽  
Prolonged Aptt ◽  
Pharmacologically Active

<p>The study evaluated anticoagulant properties of the aqueous extract of <em>Cestrum nocturnum</em> using aPTT-Activated Partial Thromboplastin Time, PT- Prothrombin Time &amp; TT-Thrombin Time as standard procedures.</p><p>For <em>in vitro</em> coagulation assays, aqueous extract of plant prolonged APTT, TT, and PT clotting times in a dose-dependent manner (Table 7). It prolonged APTT clotting time from 45 ± 2 (2mg/mL) to 82.2 ± 2.63s (10mg/mL), PT clotting time from 20.4 ± 1.49 (2mg/mL) to 31.4 ± 2.15s (10mg/mL), and TT clotting time from 9.2 ± 1.16 (2mg/mL) to 17.4 ± 1.01s (10mg/mL) at the concentration of 2 to 10mg/mL. Heparin prolonged APTT and PT clotting times more than 111.8s and 40.8s, respectively, at a concentration of 1 IU/mL. Heparin prolonged TT clotting times more than 20.6s at a concentration of 1 IU/mL.</p><p>The phytochemical screening of the plant confirm the presence of saponin in the water and ethanolic extract, Alkaloid in all the extract except hexane extract, tannin in water, ethanol and methanol extract, amino acid in water and ethanolic extract, carbohydrate in water and methanolic extract and triterpenoids and glycoside were absent in all the extracts. The results demonstrated that the aqueous extract of <em>Cestrum nocturnum</em> possesses pharmacologically active anticoagulant principles that could be isolated and evaluated for clinical or physiological purposes.</p>

scholarly journals A Original Article ANALGESIC ACTIVITY OF BARK AND LEAVES OF FICUS RELIGIOSA L. FROM NEPAL

2020 ◽  
pp. 32-35
Author(s):  
DHARMA RAJ MARASINI ◽  
JITENDRA PANDEY ◽  
LAXMAN PAUDEL SHARMA ◽  
LAXMI PAUDEL ◽  
ROSHNI GYAWALI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Test Group ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Latency Time ◽  
Hot Plate ◽  
Ficus Religiosa ◽  
Standard Drug ◽  
Ethanolic Extracts

Objective: Because of adverse side effects, caused by NSAIDs, tolerance, and dependence induced by opiates, the use of these analgesic agents has not been successful in all cases. Therefore, alternative analgesic drugs from plant sources are the new target now days. The objective of this study was to evaluate the analgesic activity of ethanolic extracts of stem barks and leaves of Ficus religiosa. Methods: The analgesic activity of ethanolic extract of stem barks and leaves was evaluated in the Swiss albino mice model using acetic acid-induced writing response and Eddy’s hot plate method. Analgesic activity was demonstrated with the percentage inhibition of acetic acid induced writings and the percentage increased in latency time of paw licking. The potency of test extracts was compared with standard drug, Diclofenac. Results: Ethanolic extract of leaves and bark of F. religiosa showed potential analgesic activity from both methods. From Eddy’s hot plate model, it was observed that the percentage of increased latency time at 90 min by ethanolic extract of leaves and stem bark was found to be 70.81 % (8.54 min) and 70.78 % (8.53 min) respectively at a dose of 400 mg/kg. Both of these results are statistically significant (p<0.05) as compared to the test group. Furthermore, both of these extracts showed the dose-dependent and time-dependent increased in latency time and these results are compared to that of standard drug Diclofenac. Similarly, ethanolic extract of leaves and stem at 400 mg/kg significantly inhibited the number of writhings induced by acetic acid. The percentage inhibition of writhings by ethanolic extract of leaves at a dose of 400 mg/kg was 68.47 % which was similar to that of standard drug Diclofenac (68.47 %). However, ethanolic extract of bark showed relatively lower percentage inhibition (60.79 %) as compared to leaf extract and standard, but the result was significant as compared to that of the test group (p<0.05). Conclusion: Ethanolic extracts of F. religiosa stem bark and leaf possess both central and peripheral analgesic properties and these effects may be beneficial for the management of pain.

scholarly journals Hepatoprotective Activity of Curcuma mangga Extract on Paracetamol-Induced Male Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 35-40
Author(s):  
Yuandani ◽  
Silvia Mardaliza ◽  
Marianne
Keyword(s):  
Ethanol Extract ◽  
Serum Levels ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Hepatoprotective Effect ◽  
Hepatic Tissue ◽  
High Dose ◽  
Male Mice ◽  
Mice Liver

This study was carried out to investigate the protective effect of ethanol extract of Curcuma mangga rhizomes on paracetamol-induced hepatotoxicity. High dose of paracetamol (1.35g/kg bw) was used to induce hepatic necrosis of mice liver. The male mice  received ethanol extract of C. mangga rhizomes (100, 200 and 400 mg/kg BW) for 7 days. The hepatoprotective actvity of extract was compared to normal, positive (curcuma) and negative control. The liver function was evaluated by measuring the biochemistry parameters which include alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, histophatological study on hepatic tissue section was also carried out. The C. mangga extract displayed hepatoprotective effect except at dose of 100 mg/kg bw. The increasing of serum levels of AST and ALT were inhibited after treatment with ethanol extract at doses of 200 and 400 mg/kb bw which was comparable with normal and curcuma as postive control (p>0.05). In addition, histological assessment of hepatic tissue demonstrated no liver damage, specially at dose of 400 mg/kb BW. The result indicate that ethanol extract of C. mangga rhizomes has hepatoprotective effect, especially at doses of 200 and 400 mg/kg bw .   Keywords: C. mangga, rhizomes, biochemistry parameters, histopathology

scholarly journals Hibiscus sabdariffa extract as anti-aging supplement through its antioxidant and anti-obesity activities

2020 ◽  
Vol 7 (1) ◽  
pp. 3572-3578
Author(s):  
Zuliana Muhamad Ridzwan Krishnamurthy ◽  
Nurzalina Abdul Karim Khan ◽  
Insathe Mohd Ali ◽  
Mohamad Dayoob ◽  
Shahad Shakho Hussein
Keyword(s):  
Aqueous Extract ◽  
Pancreatic Lipase ◽  
Healthy Aging ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
The Body ◽  
Hibiscus Sabdariffa ◽  
Ethanolic Extracts ◽  
Alpha Glucosidase

Introduction: Imbalance between total energy intake and expenditure causes accumulation of excess fat and sugar in the body which leads to development of diabetes mellitus type II, obesity, and metabolic syndrome. These harmful diseases accelerate aging and cause fatal metabolic disorders as people age. Inhibition of pancreatic lipase, and alpha glucosidase digestive enzymes is a step that can reduce excess fat and sugar from the body, which is an essential component of healthy aging. Methodology: In this study, aqueous and 50% ethanolic extracts of Hibiscus sabdariffa were investigated for their inhibitory activities on pancreatic lipase and alpha-glucosidase, in addition to their antioxidant activities (using UV-vis spectrophotometer). Results: Both extracts displayed antioxidant properties, indicated by IC50 of 5166.80 mg/mL for H. sabdariffa aqueous extract and 2809.10 mg/mL for H. sabdariffa 50% ethanolic extract. The extracts also suppressed the activities of pancreatic lipase and alpha-glucosidase enzymes, which suggests possible antiobesity and anti-diabetic activities. H. sabdariffa aqueous extract inhibited pancreatic lipase activity with IC50 of 167.5+/-12.7 mg/mL, whereas H. sabdariffa 50% ethanolic extract inhibited the enzyme with an IC50 of 790.65+/-16.02 mg/mL. Both H. sabdariffa aqueous and ethanolic extracts also successfully inhibited alpha-glucosidase enzyme activity with IC50 949.88 +/-10.83 mg/mL, and 378.33 +/-4.20 mg/mL, respectively. Conclusion: Taken together, the outcome of the investigations offers the possibility of the extracts as an anti-obesity, anti-diabetic, anti-metabolic and anti-aging agent, which can be developed into supplements for adults to prevent the occurrence of these prevalent diseases and delay the onset and effects of aging.  

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