scholarly journals Evaluation of the analgesic activity of the water soluble extract of stem of Tinospora cordifolia in experimentally induced pain in albino rats

2019 ◽  
Vol 7 (3) ◽  
pp. 938
Author(s):  
Sumanlata . ◽  
Akanksha Suman ◽  
Rajeev Kumar Sharma ◽  
Meenakshi Jindal ◽  
Adnan Khan
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Water Soluble ◽  
Tinospora Cordifolia ◽  
Albino Rats ◽  
Tail Flick ◽  
Soluble Extract ◽  
Hot Plate ◽  
Tail Flick Test ◽  
Water Soluble Extract

Background: Pain and pyrexia are the warning signals, primarily protective in nature, that cause discomfort and suffering and may even be unbearable and incapacitating. The modern drugs (like opioids, NSAIDs, corticosteroids) currently used for the management of pain, fever and inflammatory conditions, present with many known adverse effects. Tinospora cordifolia known as Giloe, widely used in folk medicine due to its property to cure a number of diseases. Hence the present study was undertaken to explore the analgesic activity of water-soluble extract of stem of T. cordifolia in albino rats in experimentally induced pain.Methods: Present study was done in the department of pharmacology, albino rats were used to study the analgesic activity of T. cordifolia aqueous extract at the dose of 1.25g/kg,2.5g/kg and 5g/kg p.o. Various methods like Eddy’s hot plate, tail flick test and acetic acid induced writhing were used for the anti- nociceptive study.Results: In Eddy’s hot plate and tail flick test an increase in reaction time was observed with peak effect at 90min. Results were similar to the standard drug Tramadol in acetic acid induced writhing increase in time of onset, decrease in number and duration of writhing was observed.Conclusions: Aqueous extract of T. cordifolia was effective in all the three models of pain suggesting its possible action by central and peripheral mechanisms. Activity of T. cordifolia can be attributed to various phytoconstituents viz. protoberberine alkaloids, terpenoids, glycosides and polysaccharides. It can be developed as potent analgesic agent in future.

scholarly journals Analgesic activity of allopurinol and febuxostat in experimental animals

2018 ◽  
Vol 7 (4) ◽  
pp. 603
Author(s):  
Yajnesh P. Sahu ◽  
Sachchidanand Pandey ◽  
Sabita Mohapatra
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Physical Dependence ◽  
Experimental Pain ◽  
Opioid Analgesics ◽  
Analgesic Nephropathy ◽  
Tail Flick ◽  
Tail Flick Test ◽  
Analgesic Effects ◽  
Peripheral Analgesia

Background: Currently, two classes of analgesics, nonsteroidal anti-inflammatory drugs (NSAIDs) and opioid analgesics are used to manage pain in different clinical situations. Chronic uses of these drugs have various adverse effects like gastric ulceration/bleeding, analgesic nephropathy and respiratory depression, physical dependence, addiction, respectively. Xanthine oxidase inhibitors, used for chronic gout, might have a role in alleviation of pain, as per literature survey. Hence, the present study was carried out to evaluate the potential analgesic activity of allopurinol and febuxostat in different experimental models.Methods: The analgesic activity of allopurinol and febuxostat was assessed by employing two different experimental pain models-tail flick latency model in rats for central analgesia and acetic acid induced writhing model in mice for peripheral analgesia and was compared with tramadol and aspirin.Results: Allopurinol and febuxostat produced significant central and peripheral analgesic effects as is evident from increase in reaction time in tail flick test and inhibition in number of writhes in acetic acid induced writhing test.Conclusions: The results of the present study demonstrate marked analgesic effect of allopurinol and febuxostat.

scholarly journals Does an Alteration in Nociceptive Response to Mineral Components of Dental Composites Involve Changes in Oxidative Status? a Brief Report

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Natalija Arsenijevic ◽  
Jovana Milenkovic ◽  
Pavle Milanovic ◽  
Aleksandra Arnaut ◽  
Milica Jovanovic ◽  
...  
Keyword(s):  
Calcium Phosphates ◽  
Dental Composites ◽  
Albino Rats ◽  
Tail Flick ◽  
Nociceptive Response ◽  
Thermal Stimulus ◽  
Hot Plate ◽  
Tail Flick Test ◽  
Simultaneous Application ◽  
Mineral Components

Abstract Since that use of bioactive mineral components of dental composites have been accompanied with various toxicities, including neurotoxicity, the aim of the study was to examine the effect of chronic application of hydroxyapatite, tricalcium phosphate and amorphous calcium phosphate in nanoparticles (nHA, nTCP, nACP) to parameters of sensitivity to thermal pain stimuli. Although the systemic toxicity of those compounds is frequently attributed to an oxidative damage, we also decided to examine the potential effects of Filipendula ulmaria extract on nociception alterations induced by the nano-sized mineral components of dental composites. Forty-two Wistar albino rats were divided into control and six experimental (equal) groups that orally received either nHA, nTCP, nACP alone, or simultaneously with FU extract for 30 days. Nociceptive alterations were quantified in the hot plate and tail flick test. The chronic administration of nHA and nACP resulted in significant increase in reactivity to thermal stimulus, with no significant change observed in nTCP group when compared to the control in the hot plate test, while simultaneous application of FU extract prevented any significant alteration of time to respond. The reaction time in the tail flick test for all three groups that received only nano calcium phosphates was reduced, with no changes in the groups treated with FU extract. The results of this study confirmed that calcium phosphates of mineral components of dental composites produced hyperalgesic effects, and this side effect were significantly attenuated by antioxidant supplementation.

scholarly journals Antinociceptive and anti-inflammatory effects of Lantana camara L. extract in mice

2015 ◽  
Vol 17 (2) ◽  
pp. 224-229 ◽  
Author(s):  
T.S.C. SILVA ◽  
I.B. SUFFREDINI ◽  
E.L. RICCI ◽  
S.R.C. FERNANDES ◽  
V.JR GONÇALVES ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Lantana Camara ◽  
Tail Flick ◽  
Male Mice ◽  
Paw Edema ◽  
Hot Plate ◽  
Active Compounds ◽  
The Family ◽  
Anti Inflammatory

ABSTRACT:he Lantana camara L. belongs to the family Verbenaceae, which contains several active compounds in leaves and roots and which are reported to have medicinal and insecticidal properties. Studies of plants within the same family show the existence of anti-inflammatory activity in paw edema induced by carrageenan, serotonin and histamine and analgesic activity in the acetic acid writhing and tail-flick tests. The present study investigated whether the L. camara extract (ACE) also exerts these effects. The ACE toxicity was studied in male mice, and the percentage of mortality recorded 7 days after treatment was assessed. The ACE was evaluated as an antinociceptive agent in the hot plate, tail-flick and acetic acid writhing tests at a nontoxic dose of 1.0 g/Kg. The results showed that 1.5 g/Kg of ACE was not able to cause death, and doses of 3.0 and 4.0 g/Kg caused 50% and 60% death, respectively, in male mice. In all of the antinociceptive tests, 1 g/Kg of ACE markedly reduced responses to pain. Our findings suggest that ACE may have active anti-inflammatory and antinociceptive properties in much smaller doses than toxic.

scholarly journals A study to evaluate efficacy of Tinospora cordifolia (Guduchi) as analgesic agent using albino wistar rats as an experimental animal model

2020 ◽  
Vol 9 (2) ◽  
pp. 242
Author(s):  
Purnendu Mandal ◽  
Asoke Kumar Das ◽  
Ananya Mandal ◽  
Tuhin Kanti Biswas
Keyword(s):  
Side Effects ◽  
Analgesic Activity ◽  
Herbal Medicines ◽  
Tinospora Cordifolia ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Tail Flick ◽  
Standard Drug ◽  
Tail Flick Latency

Background: Pain is a very well-known symptom of many diseases and analgesics are used to relieve pain. The main problem with these drugs remains that of side effects. Herbal medicines are better in view of their cultural acceptability, better compatibility with human body systems and lesser incidence of side effects. Extract of Tinospora cordifolia (Guduchi) plant have been traditionally used to treat pain in traditional medicine.Methods: Commercially available preparation of T. cordifolia plant has been used as test drug (aqueous extract). Healthy albino rats of either sex, weighing between 140-200 g were selected for the study, divided into 4 groups of 6 each (control, standard, 100 mg/kg, 300 mg/kg). Central analgesic activity was assessed by tail flick model (morphine as standard drug I.P). Acetic acid 1% 10 ml/kg aqueous solution I.P. was used for abdominal writhing model. Diclofenac 150 mg/kg oral as standard drug for assessment of peripheral analgesic activity. Results were analysed using SPSS version 16 and Microsoft office excel 2007.Results: T. cordifolia extract significantly increased the tail flick latency time (sec) (mean tail flick latency control, T100, T300 6.833±0.25 sec, 8.65±017 sec, 10.01±0.14 respectively) (p value control vs T100, T300 at 90 min, 120 min, 0.0573, 0.0198, 0.0198 in between group) and decreased number of abdominal writhing in comparison with the control group (p value <0.0001).Conclusions: Extract of T. cordifolia was found to possess analgesic activity and also exhibited dose and time dependant increase involving central and peripheral mechanisms. The analgesic activity of T. cordifolia found to be comparable to standard drug used.

scholarly journals Onosma bracteatum wall: A Potent analgesic agent

2018 ◽  
Vol 17 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Hina Imran ◽  
Atiq Ur Rahman ◽  
Tehmina Sohail ◽  
S Intasar H Taqvi ◽  
Zahra Yaqeen
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Latency Period ◽  
Maximum Effect ◽  
Tail Flick ◽  
Significant Activity ◽  
Tail Flick Test ◽  
Writhing Test ◽  
Post Feeding

Background: This study was aimed to find out the central and peripheral analgesic activity of hydro methanolic extract of aerial parts of Onosma bracteatum.Material and methods: The central and peripheral analgesic activity is evaluated by tail flick test and acetic acid induced writhing test at the doses of 50, 100, 250 and 500mg/kg body weight respectively in animal models.Results: The results obtained from Tail flick test revealed that O. bracteatum possesses potent analgesic effects by inducing significant increase in latency period in dose dependent manner at all doses at 1, 2 and 3 hours post feeding respectively. The maximum effect was observed at a dose of 500mg/kg i.e. 258.9% (p<0.05) at 3hrs post feeding. Diclofenac sodium (5mg/kg body weight) run as standard also increased the latency period continuously and highest activity was noted at 3hr i.e. 284.5% (p<0.05). Acetic acid induced writhing test also showed significant activity in a similar manner by O. bracteatum i.e 54% (p<0.05) at 500mg/kg while standard drug Diclofenic sodium (5mg/kg body weight) showed 45.9% (p<0.05) activity.Conclusion: It is concluded that O. bracteatum possesses significant central and peripheral analgesic activity in animal model.Bangladesh Journal of Medical Science Vol.17(1) 2018 p.36-41

scholarly journals AN AN STUDY OF ANALGESIC ACTIVITY OF HAFFNER’S TAIL CLIP METHOD ON ALBINO WISTAR RATS OF SOME POLYHERBAL FORMULATION

2019 ◽  
pp. 142-144
Author(s):  
LOKENDRA SINGH
Keyword(s):  
Analgesic Activity ◽  
Side Effect ◽  
Analgesic Effect ◽  
Design Method ◽  
Curcuma Longa ◽  
Albino Rats ◽  
Tail Flick ◽  
Herbal Drugs ◽  
Tail Flick Test ◽  
Tail Clip

Objective: Herbal drugs are more beneficial better than aspirin because this is an herb so no side effect this drug and it is easy to collect or use to as herbal drugs. Words are inadequate to describe the motivation for my work given to my beloved guide. I would like to add special thanks to my guide Gauravbilwal, for their guidance, support, and encouragement. Purpose (Hypothesis): The main purpose of this article pays to attention for herbal drugs because they are naturally old effective drugs. As well as, Ayurveda treatment is very older effective technique. Design/Method: Haffner gave to this technique of determining analgesic are around in 1929. Procedure: This technique according to tail if clipped with any object and tightly or will be compression generation of pain in the tail as well as mice starting to bite that portion of its tail, and could evaluate and recorded the response how much it bites tail quickly or in potential. Using this simple yet important marvel, we may apply the drug to be evaluated and record the response whether it bites tail quickly or in potential. If given drugs have analgesic likely, then rat will not bite its tail so frequently. Mice that do not show any response within 15 s will reject from the experiment. Results: The found in analgesic activity of additional compounds test to significant on tail flick test than acetic acid-induced test and thus it appears that the test compounds inhibit predominantly the peripheral pain mechanism. The results of the study indicate that the extracts of polyherbal plants of analgesic activity by reducing the abdominal constriction significantly and may supposed to have a possible role in inhibition of cyclooxygenase in the prostaglandin pathways (p****<0.0001, ***0.0001, *0.05). Conclusion: The present study showed the significant analgesic effect of both aqueous and alcoholic at 400 mg/kg doses in albino rats, we reported for the 1st time analgesic effect of different plants (Curcuma longa, Colchicaceae, Colocynthis, Withania somnifera, and Achyranthes aspera) in Haffner’s tail clip models. Aspirin has each uncoated effervescent tablet content are acetylsalicylic acid I.P. 325 mg. Finally summarized in this article represent a most effective results of herbal drugs equalized allopathic drugs without any other side effect. Hence, this is very usefully combination of Ayurveda drugs.

Syntheses and Evaluation of the Analgesic Activity of Some 4-Acetyl- 4-phenylpiperidine and 4-Hydroxy-4-phenylpiperidine Derivatives

1999 ◽  
Vol 54 (10) ◽  
pp. 1327-1336 ◽  
Author(s):  
Zafar Saeed Saify ◽  
Khalid Mohammed Khan ◽  
Syed Moazzam Haider ◽  
Syed Tasadaque Ali Shah ◽  
Muhammad Saeed ◽  
...  
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Tail Flick ◽  
Spectroscopic Techniques ◽  
Tail Flick Test ◽  
Derivatives Of

Synthesis and analgesic activity of 4-phenylpiperidine derivatives is a topic of high actuality. A s part of a programme towards obtaining new potential analgesics, ten derivatives of 4- acetyl-4-phenylpiperidine and ten derivatives of 4-hydroxy-4-phenylpiperidine were synthesised and characterized through spectroscopic techniques. All derivatives along with the parent compounds were evaluated for analgesic activity in the acetic acid-induced writhing assay and tail-flick test in mice. All derivatives of 4-acetyl-4-phenylpiperidine except one com pound exhibited more or less protection against mice writhing, whereas all the compounds proved to be inactive in the tail-flick test at doses of 50 and 100 mg/kg body weight.

scholarly journals Analgesic and Anti-Inflammatory Activities of Quercetin-3-methoxy-4′-glucosyl-7-glucoside Isolated from Indian Medicinal Plant Melothria heterophylla

Medicines ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 59 ◽  
Author(s):  
Arijit Mondal ◽  
Tapan Kumar Maity ◽  
Anupam Bishayee
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Tail Flick ◽  
Test Compound ◽  
Active Components ◽  
Hot Plate ◽  
Rat Paw Edema ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Cox 1

Background: Melothria heterophylla (family: Cucurbitaceae), commonly known as kudari, is used in the Indian traditional medicine to treat various inflammation-associated diseases, such as asthma, arthritis and pain. However, the anti-inflammatory active components of this plant have not been identified yet. The aim of this study was to investigate the potential analgesic and anti-inflammatory activities of a compound, quercetin-3-methoxy-4′-glucosyl-7-glucoside, isolated from M. heterophylla. Methods: The anti-inflammatory activity was determined using carrageenan- and dextran-induced rat paw edema as well as cotton pellet-induced granuloma in rats, whereas the analgesic activity was analyzed using acetic acid-induced writhing, hot plate and tail flick response in mice. The test compound was orally administered at a dose of 5, 10 or 15 mg/kg. The cyclooxygenase-1 (COX-1)- and COX-2-inhibitory capacity of the test compound was studied by enzyme immunosorbent assay. Results: Quercetin-3-methoxy-4′-glucosyl-7-glucoglucoside at 15 mg/kg exhibited a maximum inhibition of carrageenan-induced inflammation (50.3%, p < 0.05), dextran (52.8%, p < 0.05), and cotton pellets (41.4%, p < 0.05) compared to control animals. At the same dose, it showed a 73.1% inhibition (p < 0.05) of the pain threshold in acetic acid-induced writhing model. It also exhibited a considerable analgesic activity by prolonging the reaction time of the animals based on hot plate as well as tail flick response. The test compound was found to inhibit COX-1 (IC50 2.76 µg/mL) and more efficiently, COX-2 (IC50 1.99 µg/mL). Conclusions: Quercetin-3-methoxy-4′-glucosyl-7-glucoside possessed substantial analgesic and anti-inflammatory activities possibly due to inhibition of prostaglandin production, supporting the ethnomedicinal application of M. heterophylla to treat various inflammatory disorders.

Analgesic activity of Hydroalcholic extract of Achyranthes aspera leaves on animal model

2021 ◽  
pp. 239-241
Author(s):  
Karunakar Shukla ◽  
Sourabh Jain ◽  
Narendra Patil ◽  
Ketan Patil ◽  
Kalpesh Wagh
Keyword(s):  
Animal Model ◽  
Reaction Time ◽  
Medicinal Plant ◽  
Analgesic Activity ◽  
Tail Flick ◽  
Analgesic Agent ◽  
Hot Plate ◽  
Tail Flick Test ◽  
Achyranthes Aspera ◽  
Albino Mice

Achyranthes aspera L. (Family: Amaranthaceae) is widely used as a medicinal plant. The hydroalcholic extract of Achyranthes aspera L. leaves was screened for its analgesic activity. The dose (200 mg/kg) was tested for analgesic activity using hot plate and Tail flick test in albino mice. The hydroalcholic extract of Achyranthes aspera L. leaves showed maximum analgesic activity in hot plate at reaction time 120 min (7.40±0.08) and tail flick method at reaction time 120 min (6.9±0.06). These study suggest that the hydroalcholic extract of Achyranthes aspera L. could be considered as potential analgesic agent.

Effects of aloperine on acute and inflammatory pain models in mice

2015 ◽  
Vol 8 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Yang Yang ◽  
Shao-Ju Jin ◽  
Hong-Ling Wang ◽  
Yu-Xiang Li ◽  
Juan Du ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Inflammatory Pain ◽  
Tail Flick ◽  
Paw Edema ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Tail Flick Test ◽  
Plate Test ◽  
Tnf Α ◽  
Anti Inflammatory

AbstractBackground and objectivesAloperine (ALO) is an alkaloid compound and presents in several medicinal plants. This study was undertaken to investigate the anti-nociceptive and anti-inflammatory activities of ALO on various chemical- and thermal-induced hypersensitivity models in mice.MethodsThe anti-nociceptive effect of ALO was evaluated using acetic acid-induced writhing test, hot plate test, tail flick test, formalin test, ear swelling test, locomotor activity test, rota-rod test and carrageenan-induced paw edema test in mice. Inflammatory cytokines including interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), interleukin-1 beta (IL-1β) and prostaglandin E2 (PGE2) expression were examined in ALO- and vehicle-treated mice.ResultsThe results showed that ALO significantly attenuated acetic acid-induced writhing numbers in mice in a dose-dependent manner. ALO showed no effect on prolonging latency in the hot plate test and the tail-flick test. ALO showed analgesic activity in the inflammatory phase of formalin-induced pain. Its anti-inflammatory effect was also confirmed in the ear-swelling test. In the carrageenan-induced paw edema model, ALO significantly and dose-dependently reduced the carrageenan-induced paw edema, decreased the contents of TNF-α IL-1β and PGE2, but increased the IL-10 production. On the other hand, ALO showed no influence on the rota-rod performance time or on spontaneous locomotor activity.ConclusionIt is concluded that ALO has both anti-inflammatory and analgesic effects, especially in the field of inflammation pain.ImplicationsOur findings support the hypothesis that ALO ameliorates inflammatory pain induced by chemical and thermal stimuli and provides a scientific basis for the resource development and clinical use of aloperine.

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