scholarly journals Mispa count X; The first indigenous indian hematology 3-part analyzer

2022 ◽  
Vol 8 (4) ◽  
pp. 265-273
Author(s):  
Deepa K Vijayan ◽  
Jiby Krishna K G ◽  
Dinimol Danniel ◽  
Ashily Shaji ◽  
Jojo Mathew ◽  
...  

: Impedance technology was a revolution in the history of Hematology. Mispa Count X is the first indigenous 3-part hematology analyzer in India, which works on the principle of impedance technology. : Performance evaluation of Mispa Count X.: The analyzer produces the measurement results of 18 parameters with throughput of 60 samples per hour. Mispa Count X was compared with benchmark analyzers Coulter DxH 800 and Sysmex XN 1000 to validate its performance. : Mispa Count X exhibited a wide linearity range for WBC, RBC, platelet and hemoglobin. The carry over for WBC, RBC, PLT and Hb was estimated and found to be well within the acceptable limits. The r values (> 0.90) and bias estimation of Mispa Count X on comparing with Coulter DxH 800 and Sysmex XN 1000 were acceptable, except for mid cell counts and for MPV. Mispa Count X exhibited good precision with an acceptable CV% (< 10%). The primary parameters of the stored samples were stable at room temperature for 24 hours. : So we conclude our study by proving that the Mispa Count X would be an affordable-reliable alternative for Indian healthcare sector instead of expensive imported hematology analyzers.

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 4731-4731
Author(s):  
H. Elizabeth Broome ◽  
Han-Inge Bengtsson ◽  
Laura Stephens ◽  
Lisa Palm

Abstract Introduction: Devices such as the CellaVision® DM96 (CellaVision AB, Lund, Sweden) locate and image nucleated cells on blood smears. Using image recognition software, the DM96 also pre-classifies those cells into differential categories similar to the most complex hematology analyzers. We compared the cell counts, differential counts and flagging information gained from a complex hematology analyzer, the XE5000 (Sysmex, Kobe, Japan), with information from a minimal hematology analyzer (Sysmex PocHi) plus the DM96. We found that the cell counts, differential and flagging capabilities are similar, but the PocHi plus DM96 advantages include allowing remote review of the blood smear. Methods: 210 blood samples, selected for various abnormalities, had complete blood counts with automated differentials produced by a Sysmex XE5000 hematology analyzer. These results were compared with cell counts from the Sysmex PocHi hematology analyzer, their 100-cell DM96 post reclassification differentials, and with DM96 pre-classification differentials using standard regression analyses and Rumke 95% confidence intervals (CI) as calculated using the Clopper-Pearson method. Flagging by the XE5000 for immature granulocytes (IG's) and for blasts/abnormal cells was compared to the DM96 pre-classification using truth tables with the DM96 post reclassification as the gold standard. The following translations were used to compare flagging: IG's > 2 for either post reclassification DM96 differential, XE5000 or the DM96 pre-classification differential; Any blast cells on the manual differential were compared to XE5000 flags WBC Abn Scg; NRBC Abn Scg; Blasts?; Atyp LY?; Abn Ly/ L_Bl? and to DM96 pre-classification % Blasts > 0%; unidentified cells >3%. . Results: Non-differential blood count parameters including white blood count, red blood count, hemoglobin, mean corpuscular volume (MCV), and platelet count showed excellent correlation between the PocHi and the XE5000 with R2>0.95. Differential-dependent blood count parameters including neutrophils, lymphocytes, monocytes, eosinophils, basophils and immature granulocytes showed excellent correlation between the XE5000 and pre-classification DM96 with R2>0.95. Nucleated red cells also showed excellent correlation between the XE5000 and the DM96 with R2>0.85. For blasts/abnormal cells, the DM96 showed 100% sensitivity and 40% specificity with 0% false negatives. The XE5000 showed 93% sensitivity and 19% specificity with 3% false negative. Two of the false negatives were shared by both instruments and were 1% blasts. Of the three false negatives with the XE5000 that were true positives with the DM96, two had 1% blasts while one had 2% blasts. For immature granulocytes (IG's), the XE5000 showed 94% sensitivity and 79% specificity with 2% false negatives. The DM96 showed 85% sensitivity and 95% specificity with 4% false negatives. All of the false negatives were for IG's < 5% Conclusions: Pairing the DM96 or a similar imaging instrument with a relatively inexpensive hematology analyzer, such as those commonly used in physician office laboratories, would provide all of the information available from expensive, complex hematology analyzers in high throughput laboratories AND allow remote review of the blood smear findings by experts. Disclosures Broome: CellaVision: Consultancy, Research Funding. Bengtsson:CellaVision AB: Employment, Equity Ownership. Palm:CellaVision: Employment, Equity Ownership.


Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3845-3845
Author(s):  
Mikael Roussel ◽  
Cyrille Benard ◽  
Béatrice Ly Sunnaram ◽  
Danielle Gerard ◽  
Jean Feuillard ◽  
...  

Abstract Hematology analyzers deliver high precision blood cell counts and a good leukocyte differential (WBCD) on normal samples. But their ability to identify and quantify abnormal cells is less good and generates a significant amount of false positive results. Routinely, about 10% to 30% of results must have manual blood film reviews, which requires considerable time and are prone to a high degree of inaccuracy, especially for the less frequent cell types (Rümke et al. 1975). In contrast, flow cytometry offers superior detection and quantification of these rare events. A Cyto Diff tube combining six antibodies (CD45, 16, 2, 36, 19 & CRTH2) analysed on a modern multicolor flow cytometer make very accurate automated WBCD feasible for abnormal samples (Feuillard J et al. ISLH 2007). The objective of the study was to evaluate the efficiency of the Cyto Diff process compared to the normal laboratory process as: The time for both methods, the labor and time savings, the relative costs of both methods including med tech time, consumables, number of residual manual review. Two Coulter LH750® hematology analysers were used for the analysis of CBC, WBCD and Reticulocyte counting. An immuno-phenotyping system, with an automatic preparator Coulter FP 1000 and an Coulter FC 500® flow cytometer were connected with a Hematology analyzer to the REMISOL data manager that requests a reflex CytoDiff tube on every sample flagged by the hematology analyzer according to the laboratory’s validation rules. The remaining samples are displayed for manual validation by an operator. The complete line is called HematoFlow. Among the 4896 non-selected CBC tests evaluated during the 10 working days of our study, 877 cases were flagged by the analyzers, reviewed manually following the normal procedure as well as analyzed on HematoFlow. Interestingly, this latter allowed: 68.8% of auto-validation by the REMISOL Data Manager, 12.8% validation directly by the operator after checking the auto-gating, 8.4% required a region readjustment before validation and finally, only 10.3% (91 of the 877-flagged samples) required further exploration because the presence of large amount of ImmGrans, Plt clumps, NRBCs, etc. In conclusion, the CytoDiff tube performs well in regular clinical lab workflow saving almost 90% of the samples flagged by the hematology analyzers for WBC abnormalities that need further exploration following current routine procedure. Our study confirmed our previous results and the fact that the standard auto-gating is set correctly needing only 8.4% of region readjustment by an operator who can be trained easily in few days. Basically, we are expecting that one operator well-trained for smear review and working on the HematoFlow line can handle the same workload as at least 3 operators at microscope stations following a current normal procedure. Furthermore, the CytoDiff approach provides additional information concerning the white blood cells in pathological context never obtained previously by cytomorphology including the detection of likely pro-inflammatory monocytes, several blast subsets, and multiple lymphocyte sub populations as well.


2017 ◽  
Vol 142 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Oh Joo Kweon ◽  
Mi-Kyung Lee ◽  
Hye Ryoun Kim

Context.— In the XN series of hematology analyzers (Sysmex, Kobe, Japan), the probability of the presence of abnormal cells is indicated by flags based on Q values. Objective.— To evaluate the Q value performance of the Sysmex XN-20 modular analyzer. Design.— The interinstrumental concordance, intrainstrumental precision, and diagnostic accuracy of Q values, with tested flags of “blasts/abnormal lymphocytes,” “atypical lymphocytes,” and “blasts,” were investigated. Results.— Absolute concordance rates in flagging between 2 analyzers ranged from 69.8% to 80.8%, and κ values ranged from 0.43 to 0.61. In samples with absolute related cell counts lower than 100/μL, the values ranged from 0.31 to 0.52. For intrainstrumental precision, standard deviations ranged from 4.8 to 23.9 for the blasts/abnormal lymphocytes, from 18.7 to 59.1 for the blasts, and from 11.0 to 23.0 for the atypical lymphocytes. Using a default Q value cutoff, diagnostic accuracy values based on the area under the curve, sensitivity, and specificity were, respectively, 0.910, 90.9%, and 72.2% for blasts/abnormal lymphocytes; 0.927, 84.9%, and 89.8% for blasts; and 0.865, 74.4%, and 84.9% for atypical lymphocytes. The diagnostic accuracy of Q values was much lower in samples with absolute related cell counts lower than 100/μL than in those 100/μL or higher. Conclusions.— Q values of the Sysmex XN-20 analyzer were found to be imprecise and irreproducible, especially with samples containing a small number of pathologic cells. Adjustments in the Q value threshold may help in the detection of these cells.


Author(s):  
Lauren N Pearson ◽  
Robert L Schmidt ◽  
Kenneth Cahoon ◽  
Christopher E Pelt

Abstract Background Total nucleated cell (TNC) count and differential are used to classify joint effusions as inflammatory or noninflammatory. Further diagnostic evaluation and management is contingent on this classification. TNC count can be measured by automated analyzers or by manual assessment using a hemocytometer. Studies have raised concerns regarding the accuracy of TNC counts measured by automated instruments, particularly in the setting of joint arthroplasty. The objective of this study was to determine whether metallosis, a complication of total hip arthroplasty in which metal debris accumulates in periprosthetic tissues and synovial fluid, is associated with inaccurate TNC counts in synovial fluid. Methods We compared the accuracy of cell counts measured by the Sysmex XN-1000 and Beckman Coulter Iris iQ200 with the gold standard of manual assessment using a hemocytometer in synovial fluid from patients with suspected metallosis and in fluid obtained from controls from patients with native joints and a history of arthroplasty for other indications. Results TNC counts produced by automated analyzers were associated with increased levels of discordance (relative to manual counts) in patients with metallosis. Metallosis was not associated with increased levels of discordance for RBC counts or WBC differentials. The Sysmex XN flagged all but 1 metallosis sample for manual verification of the results. Conclusions Automated methods are generally reliable for analysis of synovial fluid. TNC counts can be inaccurate in the context of metallosis following total hip arthroplasty. Laboratories should correlate automated cell counts with a microscopic assessment of the specimen, as recommended by instrument manufacturers.


2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Pandey U

Introduction: Postpartum haemorrhage is one of the leading causes of maternal death worldwide and it accounts for nearly one-quarter of all maternal deaths and almost half of all postpartum deaths in low-income countries. Primary postpartum haemorrhage (PPH) is the most common form of major obstetric haemorrhage. Materials and Methods: The study protocol was comprised of Consent, Measurement of Pre-delivery Hemoglobin, Administration of the Intervention, Measurement of postpartum blood loss and Measurement of Post-delivery (24-48 hours) Hemoglobin. Blood loss was measured using a calibrated drape. The drape was placed beneath the parturient buttocks and secured around her abdomen with ties. Blood loss was monitored for a minimum of one hour and was continued in the second hour in case of persistent bleeding. The drape with the collected blood was weighed on a scale. The weight of the drape and the container in which it is placed was deducted from the total recorded weight in order to obtain the weight of the blood collected in the drape. Blood loss weight in grams was converted to milliliters by dividing the figure in grams by 1.06 (blood density in grams per milliliter). Results: The cross tabulations were used to study the demographic, obstetrical and medical factors in women with obstetrical haemorrhage. Table 1 shows the selected sociodemographic characteristics of the study population. The mean age of cases and controls are 26.333.559 and 26.853.873 respectively. On comparison, they are statistically insignificant. (p=0.324). The educational, occupational and socioeconomic status was comparable between cases and controls (p >0.05). Table 2 shows Antenatal, intra-partum and post-partum data in cases and controls. Discussion & Conclusion: It is a study done in North India comparing the Oxytocin kept at room temperature with failure of maintenance of cold chain during transport and storage and the refrigerated Oxytocin. It is the common understanding and general training that Oxytocin must be stored in the refrigerator, failing which its efficacy reduces i.e. it, will not be effective in controlling PPH. During the study we compared the mean blood loss and change in hemoglobin levels in cases and control and despite the fact that major risk factor for PPH for example past history of PPH, past history of D&C, prolonged third stage labour duration, manual removal of placenta were comparable in both cases and controls, still the mean blood loss and change in hemoglobin values was more in cases than controls. This could be attributed to usage of market oxytocin which had failed cold chain maintenance resulted in less effective oxytocin in prevention of PPH, Hence causing more blood loss and drop in hemoglobin values. This shows the need of room temperature stable uterotonic drug in LMIC’s like ours. Recently room temperature stable carbetocin shows the potential as an effective uterotonic drug for the prevention of PPH. However according to various studies carbetocin cannot be used for induction or augmentation of labour so it cannot replace oxytocin fully, rather it acts as a part of collective PPH reduction strategy.


Ramus ◽  
1986 ◽  
Vol 15 (2) ◽  
pp. 105-121
Author(s):  
William W. Batstone

Sallust's style is provocative and tendentious, but does his admitted moral tendentiousness carry over into a political or partisan tendentiousness? For centuries we have heard of Sallust the partisan, Sallust the propagandist, Sallustian bias. The history of this perceived bias began at least in the age of Augustus when the anonymous writer of the Invectio in Ciceronem set stylus to wax and began his fraud. Less than a century later (before 96 A.D.) Quintilian regarded the work as genuine Sallust (I.O. 4.1.68; 9.3.89). The deception had worked; and both the fraud itself and Quintilian's acquiescence indicate a perceived anti-Gceronian bias to Sallust's writing.In the modern period, the history of perceived bias, already resisted by Voss, came to its climax in 1897 with an article by E. Schwartz which argued for a systematic and extreme anti-Ciceronian and pro-Caesarian bias and purpose to the Bellum Catilinae. The charges seemed to Schwartz necessary to explain (1) Sallust's chronology, (2) the significantly small role played by Cicero in Sallust's monograph, and (3) the report of certain rumours which implicated Cicero and Crassus and the denial of rumours which implicated Caesar. A systematic review of the arguments, however, gradually undermined or called into question the validity of most of Schwartz's points. By 1964 Syme could say that the main charges against Sallust had collapsed upon inspection. And they had; all but one.


2014 ◽  
Vol 34 (03) ◽  
pp. 322
Author(s):  
Maman Zuriwiatma ◽  
Mirwan Ushada ◽  
Guntarti Tatik Mulyati

Tempe ”Muchlar” is one the potential food indutry which all the job is pursued manually. The production process is based on the worker capacity. The main problem is the industry could not fulfi ll the production target of half-finished Tempe product of + 3.600 kg.  The research objective is to identify Capacity Constrained Worker using standard time, heart rate, profi le of mood states and recommending the improvement to increase the worker capacity. The measurement results were confi rmed using analysis of working environment. The research results indicated that Capacity Constrained Worker of Tempe Industry was the worker of peeling. It was indicated by the standard time of 5,63 detik/kg that could not fulfi ll the production target of 3.600 kg in 5 work hours. The improvement was pursued by decreasing the room temperature of working station. The improvement could increase the standard time of 5 second/kg, fulfi lling the production target and increasing the income. Finally the improvement using Buffer Management could improve the Capacity Constrained Worker in Tempe Industry “Muchlar”.Keywords: Standard time, Capacity Constrained Worker, production target ABSTRAKTempe ”Muchlar” merupakan salah satu industri pangan yang semua pekerjaannya dilakukan manual sehingga proses  produksinya sangat tergantung kapasitas dari pekerja. Permasalahan muncul karena target produksi tempe setengah jadi sebesar + 3.600 kg tidak tercapai.  Penelitian ini bertujuan untuk mengidentifi kasi Capacity Constrained Worker yang terjadi menggunakan pendekatan waktu baku, denyut jantung, profile of mood states serta menentukan perbaikan yang tepat untuk dapat meningkatkan kapasitasnya. Hasil pengukuran terhadap pekerja dikonfi rmasi dengan analisis keadaan lingkungan kerja. Dari hasil penelitian dapat ditentukan bahwa pekerja yang menjadi Capacity Constrained Worker adalah pekerja stasiun pemisahan kulit. Hal ini dibuktikan dari waktu baku yang diperoleh sebesar 5,63 detik/kg yang membuat target produksi sebesar 3.600 kg dalam 5 jam tidak tercapai. Dengan perbaikan yang dilakukan yaitu menurunkan suhu ruangan dari tinggi ke normal waktu baku pekerja dapat meningkat menjadi 5 detik/kg dan target produksi dapat tercapai serta meningkatkan pendapatan. Perbaikan dengan menerapkan buffer management dapat mengurangi stasiun kerja yang menjadi constraint. Kata kunci: Waktu baku, Capacity Constrained Worker, target produksi


2020 ◽  
Vol 4 (2) ◽  
pp. 216
Author(s):  
Rohmat Tulloh ◽  
Dadan Nur Ramadan ◽  
Dendi Gusnadi

E-KMS Application for Data Collection and Recapitulation of Toddler Growth in Posyandu Mekar Arum 18Abstract. Mekar Arum Posyandu 18, which located in Lengkong village, has a low to moderate participation rate of mothers and toddlers, ranging from 15% to 25%. Besides, the registration of growth and development of infants is done manually or handwritten by the cadre on a Kartu Menuju Sehat (KMS). KMS loss often occurs so that parents and Posyandu officials have difficulty finding a history of growth and development of infants. The purpose of this activity is to increase the number of toddlers who come to the Posyandu. Besides that, this activity intends to make it easier for parents of toddlers to get information about Posyandu activities schedules, data on children's growth and development, health information also Posyandu programs. While for Posyandu cadres, this activity can help to collect data and recapitulate the growth of children and also make it easier to reports to the village office or Puskesmas. This activity uses the technology implementation method through the creation of the Posyandu mobile application (mPosyandu). This application is an electronic KMS (e-KMS) based on Android. Electronic-based data collection methods are used as a basis for planning and implementing activities. From the measurement results, this community service activity can meet all the indicators of success. Those indicators are including, the number of mothers of toddlers who use the application reaches 90%, the number of toddlers who come to Posyandu has increased to 90%, all toddlers in Posyandu Mekar Arum 18 recorded digitally, the level of high satisfaction society and the level of suitability of activities that reach 100%.Keywords: Posyandu, e-KMS, toddler.Abstrak. Posyandu Mekar Arum 18 yang berlokasi di desa Lengkong memiliki tingkat partisipasi ibu dan balita yang terbilang rendah hingga sedang, berkisar antara 15 sampai dengan 25 persen. Selain itu pencatatan tumbuh kembang balita dilakukan secara manual atau ditulis tangan oleh kader pada selembar Kartu Menuju Sehat (KMS).  Kehilangan KMS sering terjadi sehingga para orang tua dan petugas posyandu kesulitan mencari riwayat tumbuh kembang balita. Tujuan dari kegiatan ini adalah untuk meningkatkan jumlah balita yang datang ke posyandu. Disamping itu kegiatan ini ditujukan untuk memudahkan orang tua balita untuk mendapatkan informasi tentang jadwal kegiatan posyandu, data tumbuh kembang anak dan informasi kesehatan serta program-program posyandu. Sedangkan bagi kader posyandu, kegiatan ini dapat membantu untuk pendataan dan rekapitulasi pertumbuhan balita dan juga dapat memudahkan dalam pelaporan ke kelurahan atau puskesmas. Kegiatan ini menggunakan metode penerapan teknologi melalui pembuatan aplikasi mobile Posyandu (mPosyandu). Aplikasi ini merupakan sebuah elektronik KMS (e-KMS) berbasis android. Metode pendataan berbasis elektronik digunakan sebagai landasan perencanaan dan pelaksanaan kegiatan. Dari hasil pengukuran, kegiatan pengabdian masyarakat ini mampu memenuhi semua indikator keberhasilan diantaranya, jumlah ibu balita yang memakai aplikasi mencapai 90%, jumlah balita yang datang ke posyandu meningkat hingga 90%, semua balita di posyandu mekar arum 18 sudah terdata secara digital, tingkat kepuasan masyarakat yang tinggi dan tingkat kesesuaian kegiatan yang mencapai 100%.Kata Kunci: Posyandu, e-KMS, Balita.


2020 ◽  
Vol 5 (2) ◽  
pp. 138-145
Author(s):  
Ely Kurniati ◽  
Rusnawati

Preeclampsia complicates 4-7% of pregnancies and remains the leading cause of maternal and fetal morbidity and mortality worldwide. Despite improvements in health care over the last decade, preeclampsia remains the second most common cause of maternal mortality in Indonesia. This study aims to determine whether the mean arterial pressure (MAP) is related to the incidence of preeclampsia in pregnant women at the Bontobangun Puskesmas, Bulukumba district for the period 2018 to 2019. This research uses a descriptive-analytic approach with Historical Cohort Studies, namely by looking at the cohort book of the history of blood pressure measurement results for pregnant women at the Bontobangun Public Health Center, Bulukumba Regency for the period 2018 to 2019. Determining the sample using the sampling method taken by purposive sampling. 31 samples of pregnant women who experienced preeclampsia and 31 controls were pregnant women with normal conditions who had certain criteria (matching) with the sample. The results explained that from the paired T-test results, the value of α> 0.037 so that it is said that there is a relationship between mean artery pressure (MAP) and the incidence of preeclampsia in pregnant women at Bontobangun puskesmas for the period 2018-2019.


1991 ◽  
Vol 05 (21) ◽  
pp. 1447-1456 ◽  
Author(s):  
A. R. HARUTUNYAN ◽  
L. S. GRIGORYAN ◽  
A. S. KUZANYAN ◽  
A. A. KUZNETSOV ◽  
A. A. TERENTIEV ◽  
...  

Two samples of benzene-treated Bi–Pb–Sr–Ca–Cu–O powder exhibited at 300 K magnetic field dependent diamagnetism and magnetization irreversibility. The treatment with benzene resulted also in the appearance of microwave absorption at low magnetic fields, while is sensitive to magnetic history of the sample. From X-ray diffraction data one can see that upon benzene treatment the reflections of 85 K and 110 K phases do not change practically, but a series of new reflections appeared, indicating a lattice modulation with 4.9 nm periodicity. A microprobe analysis revealed substantial inhomogeneity of chemical composition across the samples. The room temperature anomalies were weakened in one sample and vanished in the second upon thermal cycling.


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