scholarly journals IMPROVEMENT OF PROCEDURE OF PHAGE TITER RISE REACTION SETUP FOR INDICATION OF BACILLUS ANTHRACIS IN SOIL SAMPLES

2016 ◽  
pp. 86-91
Author(s):  
Natalya A. Feoktistova ◽  
◽  
Sergey N. Zolotukhin ◽  
Dmitriy A. Vasilyev ◽  
◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Soil Samples ◽  
Phage Titer

scholarly journals Identification of Anthrax in Endemic Areas in South Sulawesi Province

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Sartika Juwita ◽  
Purwanta Purwanta ◽  
Muflihanah Muflihanah ◽  
Titis Furi Djatmikowati
Keyword(s):  
Bacillus Anthracis ◽  
Bacterial Culture ◽  
Virulent Strain ◽  
Lethal Factor ◽  
Soil Samples ◽  
Genome Chromosome ◽  
South Sulawesi ◽  
Dna Fragment ◽  
Endemic Areas ◽  
Bacillus Genome

Anthrax is a strategic and important disease because it is a zoonotic disease which caused death and difficult to eradicate because it produces spores. The aim of the study was to identify anthrax in endemic areas in South Sulawesi Province. Soil samples obtained from Gowa and Pinrang regency were examined by bacterial culture test and then positive results from bacterial culture followed by multiplex PCR. Based on the results of the isolation of 52 soil samples show 35 samples from Pinrang regency show all negative anthrax, while from 17 soil samples from Gowa regency show 2 positive samples of anthrax. Positive samples of anthrax were from Timbuseng village, Patallasang district, Gowa regency. The results continued with PCR technique using Bacillus anthracis colonies derived from positive soil samples. It showed the virulent strain of Bacillus anthracis. A virulent strain is indicated by a DNA fragment of a pXO1 plasmid encoding a toxin lethal factor (Lef) of 385 bp and a DNA fragment of a pXO2 plasmid encoding a capsule of anthrax (Cap) of 264 bp and also common bacterial markers of the Bacillus genome chromosome (Ba183) of 152 bp.

scholarly journals Flocculating Properties of a Bioflocculant Purified from Bacillus Subtilis Isolated from the Stream Sediments of Onyearugbulem Market, Akure, Nigeria

2019 ◽  
pp. 1-16
Author(s):  
F. O. Ekundayo ◽  
F. B. Omiyale ◽  
E. R. Omomo
Keyword(s):  
Bacillus Subtilis ◽  
Metal Ions ◽  
Bacillus Anthracis ◽  
Streptomyces Griseus ◽  
Stream Sediments ◽  
Soil Samples ◽  
Bacterial Isolates ◽  
Potential Source ◽  
Flocculating Activity ◽  
Ondo State

Soil samples (sediments of stream, its bank and abattoir soil) were collected from Onyearugbulem market abattoir, Akure, Ondo State, Nigeria. Bacteria were isolated from the above soil samples by dilution and pour plate methods. Screening for best bioflocculating bacteria was also performed. Effects of metal ions (such as Mg2+, Ca2+ and Al3+), temperature and pH on flocculating activities of the bioflocculant were also determined. Six bacterial isolates producing flocculating substances were isolated and the isolate with the best flocculating property was selected. The identified bioflocculant producing bacteria are Bacillus anthracis, B. subtilis, B. thuringiensis, B. cereus, Streptomyces griseus and S. somaliensis. The best bioflocculant producing bacterium was Bacillus subtilis and the flocculating activity of its bioflocculant was stimulated in the presence of Mg2+, Ca2+and Al3+. This bioflocculant was thermostable and retained more than 80% of its flocculating activity after being heated at 100ºC for 25 minutes. It had the highest flocculating activity of 85% at pH 6 with optimum bioflocculant dosage of 0.8 mL. This study suggests soil samples from Onyearugbulem market abattoir as a potential source of bioflocculant-producing bacteria with good bioflocculating properties.

scholarly journals No hints for abundance of Bacillus anthracis and Burkholderia pseudomallei in 100 environmental samples from Cameroon

2021 ◽  
Author(s):  
Hagen Frickmann ◽  
Sven Poppert
Keyword(s):  
Nucleic Acid ◽  
Bacillus Anthracis ◽  
Real Time Pcr ◽  
Environmental Samples ◽  
Plant Material ◽  
Burkholderia Pseudomallei ◽  
Soil Samples ◽  
Water Contact ◽  
Dressing Room ◽  
Proof Of Principle

Abstract Background Little is known on the abundance of the pathogens Bacillus anthracis and Burkholderia pseudomallei in environmental samples in Cameroon. Therefore, 100 respective samples were assessed in a proof-of-principle assessment. Methods DNA residuals from nucleic acid extractions of 100 environmental samples, which were collected between 2011 and 2013 in the Mapé Basin of Cameroon, were screened for B. anthracis and B. pseudomallei by real-time PCR. The samples comprised soil samples with water contact (n = 88), soil samples without water contact (n = 6), plant material with water contact (n = 3), water (n = 2), and soil from a hospital dressing room (n = 1). Results B. anthracis and B. pseudomallei were detected in none of the samples assessed. Conclusion The results indicate that at least a quantitatively overwhelming, ubiquitous occurrence of B. anthracis and B. pseudomallei in the environment in Cameroon is highly unlikely. However, the number and choice of the assessed samples limit the interpretability of the results.

scholarly journals Complete Genome Sequences of Three Bacillus anthracis Bacteriophages

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Sivan Alkalay ◽  
Sarit Sternberg ◽  
Shunit Coppenhagen-Glazer ◽  
Ronen Hazan
Keyword(s):  
Bacillus Anthracis ◽  
Complete Genome ◽  
Phage Therapy ◽  
Soil Samples ◽  
Genome Sequences ◽  
Complete Genomes ◽  
Highly Effective ◽  
Potential Use ◽  
Therapy Treatment

ABSTRACT The new highly effective Bacillus anthracis phages Negev_SA, Carmel_SA, and Tavor_SA were isolated from soil samples, and their complete genomes were sequenced and analyzed. The isolated phages have potential use in future phage therapy treatment against anthrax.

scholarly journals Natural Dissemination of Bacillus anthracis Spores in Northern Canada

2005 ◽  
Vol 71 (3) ◽  
pp. 1610-1615 ◽  
Author(s):  
D. C. Dragon ◽  
D. E. Bader ◽  
J. Mitchell ◽  
N. Woollen
Keyword(s):  
Bacillus Anthracis ◽  
Sample Selection ◽  
Soil Samples ◽  
The Body ◽  
Complete Coverage ◽  
Phenotypic Analysis ◽  
Significant Difference ◽  
Bacillus Anthracis Spores ◽  
Wood Buffalo National Park ◽  
A Site

ABSTRACT Soil samples were collected from around fresh and year-old bison carcasses and areas not associated with known carcasses in Wood Buffalo National Park during an active anthrax outbreak in the summer of 2001. Sample selection with a grid provided the most complete coverage of a site. Soil samples were screened for viable Bacillus anthracis spores via selective culture, phenotypic analysis, and PCR. Bacillus anthracis spores were isolated from 28.4% of the samples. The highest concentrations of B. anthracis spores were found directly adjacent to fresh carcasses and invariably corresponded to locations where the soil had been saturated with body fluids escaping the carcass through either natural body orifices or holes torn by scavengers. The majority of positive samples were found within 2 m of both year-old and fresh carcasses and probably originated from scavengers churning up and spreading the body fluid-saturated soil as they fed. Trails of lesser contamination radiating from the carcasses probably resulted from spore dissemination through adhesion to scavengers and through larger scavengers dragging away disarticulated limbs. Comparison of samples from minimally scavenged and fully necropsied carcass sites revealed no statistically significant difference in the level of B. anthracis spore contamination. Therefore, the immediate area around a suspected anthrax carcass should be considered substantially contaminated regardless of the condition of the carcass.

scholarly journals Isolation and identification of avirulent strains of Bacillus anthracis from environmental samples in Central Java, Indonesia

2021 ◽  
pp. 204-211
Author(s):  
Ully Apriliana ◽  
Hendra Wibawa ◽  
Endang Ruhiat ◽  
Tri Untari ◽  
Soedarmanto Indarjulianto
Keyword(s):  
Bacillus Anthracis ◽  
Soil Samples ◽  
Avirulent Strain ◽  
Isolation And Identification ◽  
Central Java ◽  
Gram Staining ◽  
Solid Media ◽  
Virulent Strains ◽  
Different Characteristics ◽  
Central Java Province

Background and Aim: Anthrax is a non-contagious infectious disease caused by Bacillus anthracis. The bacteria form spores that are resistant to extreme conditions and can contaminate the environment for decades. This study aimed to detect and characterize B. anthracis found in endemic areas of anthrax in Yogyakarta and Central Java province, Indonesia. Materials and Methods: Soil samples were collected from Gunungkidul regency, Yogyakarta province (n=315) and Boyolali regency, Central Java province (n=100). Additional soil samples (n=10) and straw samples (n=5) were obtained from Pati regency, Central Java province. The isolation and identification of B. anthracis were performed using conventional methods: Morphology of bacteria colony in solid media, Gram staining, capsule staining, spores staining, and motility test. Isolates were further identified using polymerase chain reaction (PCR) against Ba813, lef (pXO1), and capC (pXO2) gene. An avirulent vaccine strain of B. anthracis (strain 34F2) was used as a control. Results: Only four samples grew on blood agar with a ground-glass appearance, white-gray colony (Gunungkidul and avirulent strain) or yellowish (Boyolali and Pati). All were Gram-positive, presented chains, square-ended rods, spores, and were then identified as B. anthracis. Boyolali, Pati, and avirulent strain isolates had slightly different characteristics, including the growth of non-mucoid in the bicarbonate agar medium, and their uncapsulated form. The PCR showed two Gunungkidul isolates which amplified three genes, including Ba813, lef, and capC. Contrarily, the other isolates did not amplify the capC gene. Conclusion: Gunungkidul isolates were identified as virulent strains of B. anthracis while Boyolali and Pati isolates were proposed as avirulent strains. This is the first report of isolation and identification of avirulent strains of B. anthracis in Central Java, Indonesia.

scholarly journals Bioindication of Bacillus anthracis in soil samples

2016 ◽  
pp. 55-60
Author(s):  
Е. I. Кlimushkin ◽  
◽  
N. А. Feoktistova ◽  
D. А. Vasilyev ◽  
◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Soil Samples

scholarly journals Novel multiplex-PCR for rapid detection of Bacillus anthracis spores present in soils of Sirajganj district in Bangladesh

2015 ◽  
Vol 26 (1) ◽  
pp. 67-70 ◽  
Author(s):  
KHMNH Nazir ◽  
J Hassan ◽  
SMZH Chowdhury ◽  
MB Rahman
Keyword(s):  
Bacillus Anthracis ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Polymyxin B ◽  
Soil Samples ◽  
The Other ◽  
Step Method ◽  
Bacillus Anthracis Spores ◽  
Pcr Method ◽  
Acetate Agar

Bacillus anthracis spores were isolated and detected from soil samples (n=72) using multiplex-PCR method. The bacteria were isolated and primarily identified as Bacillus anthracis using selective Polymyxin B - Lysozyme - EDTA - Thallous acetate agar. A multiplex-PCR method targeting three genes; rpoB of genome, pag of pX01 and cap of pX02 was done to confirm the isolated bacteria. Among 72 soil samples, the viable B. anthracis spores could be extracted from 14 (19.44%) samples. However, both pX01 and pX02 plasmids were harbored in 5 (6.94%) isolates. On the other hand, pX01 and pX02 was present in 8 (57.14%) and 11 (78.57%) isolates, respectively.  This two-step-method was found to be easy, accurate and rapid in identification of B. anthracis spores from soil samples and to identify the toxigenic plasmids present in this bacterium. Progressive Agriculture 26:67-70, 2015

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