scholarly journals Relıabılıty of complex ındexes for fluorescent-hyperspectral method for assessıng chlorophyll content ın plant leaves

2021 ◽  
Vol 1 (65) ◽  
Author(s):  
В. А. Касумов ◽  
К. Дж. Алиева
2021 ◽  
Vol 12 (3) ◽  
pp. 7-16
Author(s):  
Z. Bilousova ◽  
◽  
V. Keneva ◽  
Y. Klipakova ◽  
◽  
...  

To obtain the maximum yield of winter wheat, it is necessary to further optimize the existing cultivation technologies in the direction of their adaptation to changing environmental conditions. One of the areas of adaptation of plants to adverse abiotic factors is the active functioning of the photosynthetic apparatus, which depends on the amount of nutrients introduced. The influence of fertilizer application on the condition of the pigment complex of winter wheat plants in the conditions of the Southern Steppe of Ukraine has been studied. Two varieties of winter wheat were selected for the study: Shestopalivka and Mason. The experiment scheme involved the application of fertilizers at sowing (K0; K12) and foliar treatment with various tank mixtures (urea; urea + magnesium sulfate; urea + magnesium sulfate + potassium monophosphate). The pigment content has been determined by grinding fresh leaves of winter wheat, followed by the addition of a solvent in the form of acetone. Measurements of pigments were performed using a spectrophotometer. According to the research results, it was established that before the foliar treatment the a-chlorophyll content and carotenoids was higher in the plant leaves of the Shestopalivka variety. At the same time, the b-chlorophyll content on the contrary was higher for plants of the Mason variety by 17%, which may be due to the adaptation of plants of this variety to lack of light. On the 3rd day after foliar treatment, a decrease in the pigment content in the plant leaves of all experimental variants has been observed, which was due to the active growth of the photosynthetic surface and a decrease in the total dry matter mass. There was no significant difference between the varieties of the content of photosynthetic pigments in this period. On the 10th day after foliar treatment, an increase in a- and b-chlorophyll content has been observed for both studied varieties, which may be the result of adaptation of the photosynthetic apparatus of winter wheat plants to lighting conditions. Foliar treatment of winter wheat plants with a tank mixture of urea with magnesium sulfate and potassium monophosphate contributed to a further increase in the content of a-chlorophyll by 12-23%, and b-chlorophyll by 5-37% depending on the variety compared to the control. The results of the research testify to the high efficiency of complex application of nitrogen-phosphorus-potassium fertilizers for foliar treatment of winter wheat plants in the BBCH 31 stage, both against the background of pre-sowing application of potassium fertilizers and without it.


2010 ◽  
Vol 33 (2) ◽  
pp. 175
Author(s):  
Ma. Luisa España-Boquera ◽  
Philippe Lobit ◽  
Vilma Castellanos-Morales

Chlorophyll is an essential element of photosynthesis and its content in plant leaves indicates their photosynthetic capacity as well as the presence of stress or diseases. The purpose of this work was to evaluate the feasibility of estimating chlorophyll content in the Monarch Butterfly Biosphere Reserve forest (Sierra Chincua sanctuary, México) based on vegetation indices calculated by using hyperspectral reflectance measurements of plant leaves. This study focused on oyamel (Abies religiosa L.) which is the main tree specie of this area. Leaf samples were taken on 140 trees and analyzed for chlorophyll a and b, nitrogen and carbon content. The hyperspectral reflectance spectra were measured on each sample and different vegetation indices were calculated. Results showed that the indices best correlated with chlorophyll content were the red edge position index (r = 0.531) and the red edge position chlorophyll reflectance index (r = 0.506), followed by the MERIS terrestrial chlorophyll index (r = 0.497) and the green chlorophyll reflectance index (r = 0.472). Although there was a significant correlation between nitrogen and chlorophyll content, none of the indices studied here correlated with nitrogen content. The influence of various environmental factors (altitude, slope, vegetation density and aspect) on leaf composition (nitrogen, carbon chlorophyll content and chlorophyll a/b ratio) and on the vegetation indices was studied. Environmental factors had an influence on both leaf composition and vegetation indices. Chlorophyll and nitrogen content were influenced mostly by the altitude and slope of the site while vegetation indices were affected mostly by its orientation.


Lab on a Chip ◽  
2015 ◽  
Vol 15 (7) ◽  
pp. 1708-1716 ◽  
Author(s):  
Bingen Cortazar ◽  
Hatice Ceylan Koydemir ◽  
Derek Tseng ◽  
Steve Feng ◽  
Aydogan Ozcan

We demonstrate accurate measurement of chlorophyll concentration in plant leaves using Google Glass and a custom-developed software application together with a cost-effective multi-spectral leaf illuminator unit.


HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 952-957 ◽  
Author(s):  
Joshua D. Butcher ◽  
Charles P. Laubscher ◽  
Johannes C. Coetzee

Pelargonium tomentosum Jacq.; the peppermint-scented pelargonium, is an herbaceous groundcover indigenous to the Western Cape of South Africa. Volatile oils are produced by this plant, which are used in the fragrance industry. Studies on other Pelargonium species have shown chlorophyll content may affect the yield of essential oils. This study was carried out to investigate the viability of growing P. tomentosum in deep water culture (DWC) hydroponics and how best to aerate/oxygenate the nutrient solution to increase the chlorophyll content within leaves. The experiment was conducted over a period of 74 days, 16 different methods of oxygenation were applied to 9 replicates. The control had passive aeration; the treatments were made up of air-pumps, vortex oxygenators, and the application of hydrogen peroxide at various frequency intervals; these were combined with each other and run as separate oxygenation methods. The measurement of the chlorophyll content of plant leaves has been established to be an accurate way of establishing vigor, health, and levels of stress. It was found that the combination of high-frequency application (every third day) of hydrogen peroxide, vortex oxygenation, and air-pump injection (both operational for 24 hours/day) which formed treatment 11 (APVHa), yielded the highest production of chlorophyll within all the replicates differing significantly (P ≤ 0.001) from the control and other treatments.


1972 ◽  
Vol 25 (2) ◽  
pp. 215 ◽  
Author(s):  
JT O Kirk ◽  
DJ Goodchild

A polarographic technique for the study of photosynthesis in higher plant leaves, using a bare platinum electrode of the Haxo and Blinks type, is described.


2021 ◽  
Vol 328 ◽  
pp. 08003
Author(s):  
Rachmadiarti Fida ◽  
Asri Mahanani Tri ◽  
Sari ◽  
Nella Yulia ◽  
Kandilia ◽  
...  

Lead (Pb) is one of the heavy metals that causes pollution in ecosystems, heavy metal Pb can cause disturbances to humans, including plants and animals. In Indonesia, especially in Surabaya, Tabebuya grows on main roads as a shade tree as well as an ornamental plant; This plant can be exposed to Pb metal, among others, from motor vehicle exhaust. This study aimed to determine the levels of Pb in Tabebuya and to study chlorophyll in the leaves. Pb levels in plant leaves were measured using an atomic absorption spectrophotometer (AAS), and chlorophyll levels using a spectrophotometer. Data in the form of Pb and chlorophyll levels at various locations were analyzed by analysis of variance (Anova). The results showed that the location of plant species affected the content of Pb metal and there is no effect of chlorophyll content in the leaves. The results also showed that the absorption of lead (Pb) content by the tabebuia 0.25-0.44 ppm and content of chlorophyll 12.89-14, 94. Lead (Pb) content do not affect the amount of leave chlorophyll produced and the plant had capabilities in absorbing lead like pollutants (Pb).


2011 ◽  
Vol 57 (No. 4) ◽  
pp. 180-185 ◽  
Author(s):  
F. Šrámek ◽  
M. Dubský

The effect of various concentrations and ratios of iron (Fe) and manganese (Mn) chelates on growth, micronutrient uptake and chlorophyll content was investigated in a glasshouse experiment using potted petunia plants. The plants were cultivated in a peat substrate amended with dolomitic limestone at rate of 3.0 g/L (control substrate) or 12.0 g/L. The higher rate was established both to restrict the uptake of Fe, Mn and other nutrients and also to test the effectiveness of various Fe and Mn treatments. The plants in all the treatments were fertigated at weekly intervals with a nutrient solution containing macronutrients and micronutrients. Various chelate forms of Fe and Mn were used with the exception of two treatments where these two elements were omitted. The effects of different substrate pH levels (derived from different limestone contents) had a large effect on plant growth, chlorophyll content and content of Fe and Mn accumulated in the plant leaves. The plants in the high-limestone substrate devoid of Fe and Mn had reduced growth and lower chlorophyll content. They also had lower leaf Fe and Mn content than the control plants in the R3 substrate. Regular fertigation with a nutrient solution containing Mn and Fe improved plant growth rate and also increased chlorophyll content. However, its efficiency depended on the chelate form and concentration used. The effect of chelate application on the Fe and Mn leaf content was unclear as it only marginally increased leaf Fe absorption in some treatments. No effect of the various Fe/Mn ratios was observed.


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