Reticulocyte Parameters in Microcytic Hypochromic Anaemias Using Beckman Coulter DxH800 – A Study from Kerala, India

2021 ◽  
Vol 8 (14) ◽  
pp. 888-892
Author(s):  
Blessy Mary Thomas ◽  
Sheila Das ◽  
Sunil Antony ◽  
Alice David
Keyword(s):  
Bone Marrow ◽  
Iron Deficiency ◽  
Iron Deficiency Anaemia ◽  
Response To Therapy ◽  
Control Group ◽  
Blood Samples ◽  
Deficiency Anaemia ◽  
Chronic Disorder ◽  
Microcytic Hypochromic Anaemia ◽  
Hypochromic Anaemia

BACKGROUND Microcytic hypochromic anaemia is commonly due to iron deficiency, anaemia of chronic disorder [ACD] and thalassaemic syndromes. Reticulocyte count reflects the erythropoietic activity of bone marrow and is thus useful in both diagnosing anaemias and monitoring bone marrow response to therapy METHODS All samples were selected from routine blood counts, and sent for investigation of anaemia, over a period of two years. These samples were run on the DxH800 (Beckman Coulter). 385 cases were selected for the study. Blood analysis for all these cases had been requested by general practitioners to investigate anaemia. These blood samples had been collected in ethylenediaminetetraacetic acid (EDTA) anticoagulant vacutainers and processed within 2 hours of collection. Determination of red cell and reticulocyte parameters in all blood samples, was performed using the Beckman Coulter 7-part analyser [Unicell DxH 800]. RESULTS Of the 156 cases of microcytic hypochromic anaemia studied, iron deficiency anaemia (IDA) was present in 91 cases, anaemia of chronic disorder (ACD) in 50 cases, beta thalassemia trait (BTT) in 15 cases. Of the 50 ACD cases, 37 were associated with IDA. The control group comprised of 229 adult medical students (143 women and 103 men) with a median age of 18.84 ± 0.98 years. We also had 4 cases of other haemoglobinopathies, which were microcytic hypochromic, but were not included in our study as the number of cases was too less to be analysed. CONCLUSIONS New reticulocyte parameters are useful for evaluation of iron status and diagnosing iron deficiency anaemias. They also are reliable parameters for recognising subsets of anaemic patients thereby improving the management of anaemia. KEYWORDS Reticulocyte, Microcytic, Hypochromic, Anaemia, Beckman Coulter

scholarly journals Haematological Profile in Microcytic Hypochromic Anaemia in Children

2021 ◽  
Vol 9 (02) ◽  
pp. 3-7
Author(s):  
Raju Kafle ◽  
Anupama Bastola ◽  
Ashok P Samdurkar ◽  
Narayan Gautam ◽  
Anuj Poudel ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anaemia ◽  
Cross Sectional Study ◽  
Severe Anaemia ◽  
Cross Sectional ◽  
Deficiency Anaemia ◽  
Microcytic Hypochromic Anaemia ◽  
Hypochromic Anaemia ◽  
Haematological Profile ◽  
Rbc Count

INTRODUCTION: The most common disorders presenting with microcytic hypochromic anaemia are iron deficiency anaemia (IDA) and β-Thalassemia trait (β-TT), and each of them has different pathogenesis and treatment modality. Here we intend to see the haematological profile in microcytic hypochromic anaemia in children apart from IDA and Thalassemia with respect to age and gender. MATERIAL AND METHODS: A total of 95 pediatric patients between the ages of 2 to 12 years with microcytic hypochromic anaemia were included in the study. Blood samples obtained from each patient were evaluated for various haematological and biochemical profiles along with haemoglobin electrophoresis. It was a hospital based observational cross-sectional study done for a period of 18 months from January 2019 to July 2020. RESULTS: Severe anaemia was seen in 55 (57.89%) cases, followed by 38 (40.00%) cases with moderate anaemia and 2 (2.11%) cases with mild anaemia. Majority of the cases showed IDA, which were 85 (89.5%) cases, followed by 6 (6.3%) cases with β-TT and 4 (4.2%) cases with β-TM. RBC count, hematocrit and RDW showed significant variation between IDA, β-TM and β-TT. Most number of correctly diagnosed cases were shown by Ricerca Index with 90 (94.74 %). CONCLUSION: Our study concludes RBC count and RDW, along with Srivasthava Index, Ricerca Index and RDW Index could be used as reliable indices to differentiate between iron deficiency anaemia and β-Thalassemia. Red cell indices, serum iron profile and haemoglobin electrophoresis complement each other for the precise diagnosis of underlying cause of microcytic hypochromic anaemia.

scholarly journals A study to assess the effectiveness of honey dates amla mix on biochemical markers among adolescent girls with Iron deficiency anaemia

2020 ◽  
Vol 11 (2) ◽  
pp. 2288-2293
Author(s):  
Bhuvaneswari G.
Keyword(s):  
Iron Deficiency ◽  
Adolescent Girls ◽  
Biochemical Markers ◽  
Serum Iron ◽  
Iron Deficiency Anaemia ◽  
Control Group ◽  
Deficiency Anaemia ◽  
Post Test ◽  
And Control ◽  
Experimental Group

WHO Global Database on Anaemia for 1993-2005, covering almost half of the world’s population, estimated the prevalence of anaemia worldwide at 25 per cent.The aim of the study is to determine the effectiveness of honey dates amla mix on biochemical markers among adolescent girls with Iron deficiency anaemia. Quantitative experimental and control group pre and post-test design was used in this study. The study was conducted among adolescent girls in two different residential homes. Total study population is 170 adolescent girls were selected by simple random sampling technique. 85 adolescents girls were assigned to the experimental group and 85 participants in control group. For experimental group honey dates amla mix was given for 3 months whereas in  the control group iron and folic acid supplementation were provided. The study results shows that pre and post-test intervention scores of control and experimental group were compared by Wilcoxon scores rank test. Comparison of pre-test and post-test scores of severity of anemia, clinical variables, and level of fatigue and level of satisfaction, bio chemical parameters (reticulocytes, MCV, haematocrite, serum iron, serum ferritin, TIBC) were assessed in both experimental and control group. Between the experimental and control group (unpaired‘t’ test) reticulocytes, mcv, heamatocrite, serum iron serum ferritin showed significant changes in the pre test and post-test but there is no changes in TIBC. The study concluded that alternative nutritional therapy was effective for enhancing the blood heamoglobin & biochemical paramteres level, decreased the clinical variables and fatigue level among the adolescent girls with iron deficiency anemia.

Iron Deficiency Anaemia and Child Development

2005 ◽  
Vol 17 (1) ◽  
pp. 19-21 ◽  
Author(s):  
T. Hokama ◽  
M. Gushi Ken ◽  
N. Nosoko
Keyword(s):  
Child Development ◽  
Iron Deficiency ◽  
Iron Deficiency Anaemia ◽  
Speech Development ◽  
Control Group ◽  
Deficiency Anaemia ◽  
Developmental Index ◽  
The Mean ◽  
Group 2 ◽  
Group 1

A developmental test was carried out to evaluate the effect of iron deficiency anaemia on child development by using Bailey Scale of Infant Development (BSID) and Enjoji Scale of Infant Analytical development (ESID). The subjects were 54 children, divided into three groups. Group 1 consisted of 15 infants with anaemia; Group 2: 10 infants with a past history of anaemia and Group 3: 29 healthy normal infants without anaemia formed the control group. The characteristics of infants and their family background were not different among the three groups except for the male/female ratio. The mean mental developmental index (MDI) and psychomotor developmental index (PDI) of Group 1 and Group 2 were lower than that of control group using the BSID test. The mean speech development quotients of Group 1 and Group 2 were lower than that of control group in ESID. These tendencies were observed after subjects were stratified by sex. Therefore, the results of the study suggests that iron deficiency anaemia may affect child development especially speech development. Asia Pac J Public Health 2005: 17(1): 19-21.

scholarly journals Differential effects of chronic iron deficiency anaemia on junctional and labyrinthine zones of placenta in Sprague dawely rat

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Omer Awad ◽  
Abdel Malek ◽  
Julius Ogeng’o
Keyword(s):  
Iron Deficiency ◽  
Gestational Age ◽  
Iron Deficiency Anaemia ◽  
Control Group ◽  
Albino Rats ◽  
Control Groups ◽  
Junctional Zone ◽  
Deficiency Anaemia ◽  
The Difference ◽  
And Control

Iron deficiency anaemia causes adverse pregnancy outcome. Studies reveal its generalized effects on histomorphometry of the placenta, without details on specific zones nor effect of gestational age. These data are important for planning  intervention. This study was, therefore, designed to describe the histomorphometric changes associated with iron deficiency anaemia on placenta of albino rat. Fourty nine (49) Sprague – Dawely albino rats were randomly separated into experimental and control groups. The  experimental group was rendered anaemic by removing 1.5 ml of blood per bleed on five alternate days. Placentas were collected on gestational days 17, 19 and 21. Five cubic milimetre segments were fixed in 10 % buffered formaldehyde solution; dehydrated in ethanol and embedded in paraffin wax. Five micron thick sections were cut, deparaffinized and stained with Hematoxylin and Eosin. Micrographs were taken using Leica ICC 50 digital photomicrographic camera attached to a computer at magnification x40 and the thickness of the labyrinth and junctional zones measured. Student t- test was used to compare values for the   experimental and control groups. The labyrinth in the chronic anaemia group was thinner than in the control group at gestational days 17, 19 and 21. The junctional zone, on the other hand, was consistently thicker in anaemic than in the control animals. The difference in thickness of junctional zone varied with  gestational age. At gestational day 17, the zone was significantly thicker in the anaemic group (628.9 μ) than in the control (381 μ). On day 19 and 21, however, the difference was not statistically  significant. In conclusion, the effects of chronic iron deficiency anaemia on the labyrinth differ from those on the junctional zone of the placenta. This differential effect appears to depend on the function and gestational age. The decrease in thickness of the  labyrinth may be designed to maintain placental diffusion capacity while increased thickness of the junctional zone constitutes a compensatory physical and nutritional adaptation to hypoxia.Key words: placenta, labyrinthine, junctional zones, thickness, anaemia.

scholarly journals A study on influence of iron deficiency anaemia over HbA1c levels

2019 ◽  
Vol 6 (4) ◽  
pp. 1095
Author(s):  
Sankar Kalairajan ◽  
Vijaya Durairaj K. ◽  
Malathy A. R.
Keyword(s):  
Iron Deficiency ◽  
Hba1c Level ◽  
Iron Deficiency Anaemia ◽  
Control Group ◽  
Haemoglobin A1c ◽  
Deficiency Anaemia ◽  
The Mean ◽  
Ethnic Variations ◽  
Racial Ethnic ◽  
Hba1c Levels

Background: Haemoglobin A1c (HbA1c) is a glycated form of haemoglobin reflects average plasma glucose over the previous 8 to 12 weeks. HbA1c can be affected by multiple non-glycaemic parameters. Iron deficiency anaemia (IDA) is one among them which is the most common type of anaemia in India. However, reports on the effects of iron deficiency anaemia on HbA1c levels are inconsistent. Hence we conducted a study to find out the influence of iron deficiency anaemia over HbA1c levels.  Methods: 120 patients confirmed to have iron deficiency anaemia were enrolled in this study. HbA1c levels were measured at baseline and 3 months after treatment, and these values were compared with those in the control population.Results: The mean baseline HbA1c level in anaemic patients (4.62%) was significantly lower than that in the control group (5.45%, P<0.001). A significant increase was observed in the patients HbA1c levels at 3 months after treatment (5.82%, P<0.001). There was a significant correlation observed between haemoglobin and HbA1c level (Coefficient of correlation=0.26, P<0.01) in the study group before correction.Conclusions: In contrast to the observations of previous studies, ours showed that HbA1c levels increased with treatment of iron deficiency anaemia. This could be attributable to nutritional deficiency, racial-ethnic variations and/or certain unknown variables. Further studies are warranted.

scholarly journals CHANGES IN SOME FRACTIONS OF CYTOKINES IN CHILDREN WITH IRON DEFICIENCY ANEMIA

2021 ◽  
Vol 21 (3) ◽  
pp. 82-87
Author(s):  
V.A. Najafova
Keyword(s):  
Iron Deficiency ◽  
Correlation Coefficient ◽  
Serum Ferritin ◽  
Iron Deficiency Anaemia ◽  
Deficiency Anemia ◽  
Control Group ◽  
Average Correlation ◽  
General Group ◽  
Deficiency Anaemia ◽  
Tnf Α

The aim of the study was to investigate the effect of iron deficiency anaemia on certain cytokine fractions (IL-2, INF-γ, TNF-α) in children aged 6 months to 5 years in Azerbaijan. Methods. The study involved 123 children. According to the hematological and biochemical blood parameters of children aged 6 months to 5 years, 95 children (58 boys, 37 girls) were diagnosed as having anaemia of varying degrees: 32 children had mild iron deficiency anaemia, 37 children had moderate iron deficiency anaemia, and 26 children had severe iron deficiency anaemia. Results. The study demonstrates the level of INF-γ is lower in the general group of children with iron deficiency anaemia compared to the control group (2.5±1.2 pg/ml and 3.9±1.6 pg/ml, respectively). In the general group of children with iron deficiency anaemia, the average correlation coefficient (r=+0.7) between INF-γ with haemoglobin and with serum ferritin (r=+0.6) has been shown. Taking into account the weakening of INF-γ in children with iron deficiency anaemia during the study period, we investigated the annual incidence of acute respiratory viral infections among the general group of children with iron deficiency anaemia and the control group (63.2% and 25%, respectively). There were no significant changes in TNF-α value ​​in children of the general group with iron deficiency anaemia compared to the control group (2.7±1.5 pg / ml, 2.7±1.1 pg / ml, respectively). The IL-2 index in the general group was lower than in the control group (1.6±1.0 pg / ml, 2.9±1.6 pg / ml, respectively). Children in the general group with iron deficiency anaemia had an average correlation coefficient (r=+0.65) IL-2 with haemoglobin and a high correlation coefficient with serum ferritin (r=+0.8). The incidence of pneumonia in the group of children with iron deficiency anaemia was 17.9%. Conclusion. The obtained lowered results of İNF-γ and İL-2 in comparison with the control group and an increased incidence of respiratory diseases indicate a possible reduction in cellular immunity.

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