scholarly journals Experimental Validation of the Use of Immobilized Form of Miramistin in the Treatment of Advanced Peritonitis

2021 ◽  
Vol 14 (1) ◽  
pp. 53-60
Author(s):  
Boris Semenovich Sukovatykh ◽  
Maria Alekseevna Zatolokina ◽  
Anastasiya Viktorovna Mosolova
Keyword(s):  
Aqueous Solution ◽  
Inflammatory Process ◽  
Comparison Group ◽  
Abdominal Cavity ◽  
Inflammatory Activity ◽  
Sodium Carboxymethylcellulose ◽  
Generalized Peritonitis ◽  
Fecal Suspension ◽  
Anti Inflammatory ◽  
Experimental Group

Introduction. Mortality in generalized peritonitis ranges from 16% to 30%.The aim of research was to experimentally study the effect of the immobilized form of 0.01% miramistin on the basis of sodium salt of carboxymethylcellulose on the course of the inflammatory process in generalized purulent peritonitis.Materials and methods. The experimental study included 288 male Wistar rats, divided into 3 groups, 96 animals each. Animals of the 1st group (control) were exposed to laparotomy and lavage of the abdominal cavity with saline under aseptic conditions 24 hours after the introduction of fecal suspension into the abdominal cavity. Simultaneously, animals of the 2nd group (comparison) underwent a thorough sanitation of the abdominal cavity with saline with removal of purulent effusion and fibrin films at the first stage, and at the second stage, they were injected 5 ml of an aqueous solution of 0.01% miramistin. In animals of the 3rd (experimental) group, 5 ml of 0.01% miramistin gel was evenly distributed over the entire surface of the peritoneum after laparotomy and sanitation of the abdominal cavity with saline solution. The anti-inflammatory activity of the dosage forms was assessed by the dynamics of leukocytosis and the leukocyte index of intoxication, and the anti-inflammatory activity was assessed by the dynamics of the number of microorganisms in the abdominal exudate. The lethality of animals in each group was estimated. The following areas were taken for histological examination: small and large intestine, parietal peritoneum, pancreas, liver.Results. The anti-inflammatory activity of the immobilized form of 0.01% miramistin on the basis of sodium carboxymethylcellulose was superior to the aqueous solution of miramistin 0.01% on the 1st day - 1.3 times, on the 3rd day - 1.6 times, on the 7th day - 1.5 times. Antimicrobial activity in animals of the experimental group was 1.3 times higher on the 1st day, 1.9 times higher on the 3rd day, and 1.7 times higher on the 7th day than in the comparison group. The mortality rate in animals of the experimental group was 1.5 times lower on the 1st day, and 1.4 times lower on the 3rd and 7th days than in animals of the comparison group. On the first day, the morphological picture of peritonitis in animals of the experimental and comparison groups had no significant differences. On the 3rd day in animals of the comparison group, the inflammatory process in the abdominal cavity was pronounced, and in the experimental group, the intensity of peritonitis began to decrease, and by the 7th day it was completely eliminated.Conclusion. The results of the study allow recommending the use of the immobilized form of 0.01% miramistin based on sodium carboxymethylcellulose in the treatment of generalized peritonitis.

Anti-Inflammatory Activity of Ethanol Extract of Marine Sponge Petrosia Sp. by Supression the Level of Tumor Necrosis Factor-alpha

2021 ◽  
pp. 4435-4439
Author(s):  
Adryan Fristiohady ◽  
Muhammad Hajrul Malaka ◽  
Andi Rizqa Wahyuni Safitri ◽  
Dewo Diha ◽  
Saripuddin Saripuddin ◽  
...  
Keyword(s):  
Inflammatory Process ◽  
Oral Treatment ◽  
Ethanol Extract ◽  
The Body ◽  
Inflammatory Activity ◽  
Inflammatory Agent ◽  
Edema Volume ◽  
Group I ◽  
Tnf Α ◽  
Anti Inflammatory

Inflammation is the host's protective response to any stimulus that harms the body. Excessive inflammatory process causes tissue damage. Therefore, an anti-inflammatory agent is needed. The use of natural ingredients, especially sea sponges, is an option to reduce the side effects of anti-inflammatory agents. This utilization is related to the discovery of new agents. So, we tested the effect of the ethanol extract of Petrosia sp. as an anti-inflammatory agent. Animal induced with 1% carrageenan and left for 1 hour. After that the animals were divided into 6 groups (n = 4) and given oral treatment, namely: Group I (normal group); Group II (negative group); Group III (ethanol extract of Petrosia sp. Concentration of 0.05mg/ml); Group IV (ethanol extract of Petrosia sp. Concentration 0.1mg/ml); Group V (ethanol extract of Petrosia sp. Concentration 0.2mg/ml); and Group VI (positive group, Diclofenac Sodium). After 1 hour, the animals were measured for edema volume and plasma TNF-α levels. Based on the research conducted, the ethanol extract of Petrosia sp. decreased edema volume and plasma TNF-α levels in inflammatory mice. The concentration of 0.2mg/mL had a significant effect on the negative control used (p <0.05). On the other hand, Petrosia sp. indicates the presence of alkaloids, flavonoids, and steroids. They may play an important role in the anti-inflammatory process. Thus, it can be concluded that the ethanol extract of Petrosia sp. has anti-inflammatory activity.

scholarly journals Lichens as a source of chemical compounds with anti-inflammatory activity

2018 ◽  
Vol 64 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Elżbieta Studzińska-Sroka ◽  
Adrianna Dubino
Keyword(s):  
Animal Studies ◽  
Inflammatory Process ◽  
Folk Medicine ◽  
Chemical Compounds ◽  
Biological Properties ◽  
Inflammatory Activity ◽  
Medicinal Substances ◽  
Anti Inflammatory ◽  
Biological Actions

SummarySymptoms of inflammation accompany a number of diseases. In order to mitigate them, folk medicine has used a variety of medicinal substances, including herbs and mushrooms. Lichens are less known organisms, containing specific secondary metabolites with interesting biological properties. One of their biological actions is the anti-inflammatory activity that has been confirmed byin vitroand animal studies. It has been proven that compounds and extracts from lichens inhibit the enzymes involved in the inflammatory process. The following paper is a review of research on the little-known anti-inflammatory properties of lichens.

scholarly journals MORPHOLOGICAL CHANGES IN THE SMALL INTESTINE OF RATS WITH ACUTE GENERALIZED PERITONITIS

2019 ◽  
pp. 110-113
Author(s):  
I. Ya. Dzyubanovsky ◽  
B. M. Vervega ◽  
S. R. Pіdruchna ◽  
N. A. Melnyk
Keyword(s):  
Endothelial Cells ◽  
Small Intestine ◽  
Mucous Membrane ◽  
Morphological Changes ◽  
Histological Study ◽  
Abdominal Cavity ◽  
Inflammatory Infiltration ◽  
Generalized Peritonitis ◽  
White Rats ◽  
Fecal Suspension

The main cause of mortality in acute generalized peritonitis (AGP) is the development of multiple organ insufficiency. The intestine is the organ where the first changes develop in this pathology. The aim of the study – to research and evaluate the morphological changes in the small intestine wall of animals with experimental AGP. Materials and Methods. 32 white rats were used in this study. Acute peritonitis was modeled by introduction of 10 % fecal suspension in the dosage of 0.5 ml per 100 g of the animal's weight into the abdominal cavity of rats by puncture. The terms of observation: the 1st, 3rd and the 7th days from the beginning of the peritonitis modeling. For histological study the intestinal tissue was taken. The resulting pieces of the organ were fixed in a 10 % neutral formalin solution, which were then stained with hematoxylin and eosin. Results and Discussion. On the 3rd day of the experiment in animals with a modeled AGP, vascular changes were manifested first of all by the rounding (retraction) of endothelial cells or their desquamation and the appearance of defects, that allow plasma proteins and the formed elements of blood to leave circulation boundaries of the vascular bed. On the 7 th day in animals with a modeled AGP increased vascular permeability of the mucous membrane of the small intestine was accompanied by a significant edema of the stroma of the villi and by focal hemorrhages. Conclusion. Consequently, the distinct inflammatory changes in all terms of the injury were seen in the wall of the small intestine at the simulated AGP. The significant expansion of the capillaries and venules against the background of inflammatory infiltration in the stroma of the glandular component of the small intestine was noted on the 1st day from the beginning of the experiment. An increase in the height of intestinal villus and a crypt with retraction and desquamation of endothelial cells in the wall of vessels, which caused platelet adhesion in the areas of destruction was observed on the 3rd day. The areas of focal necrosis of the superficial epithelium, which were accompanied by multiple hemorrhages per diapedesis in the perivascular space of the mucous membrane, were seen in the wall of the small intestine of animals with a simulated AGP on the 7th day.

Anti-inflammatory properties of lyophilized inflammatory exudates

1961 ◽  
Vol 201 (6) ◽  
pp. 1155-1158 ◽  
Author(s):  
G. DiPasquale ◽  
R. J. Girerd
Keyword(s):  
Inflammatory Process ◽  
Cortisone Acetate ◽  
Inflammatory Activity ◽  
Active Fraction ◽  
Adrenal Hormones ◽  
Conditioning Factors ◽  
Adrenal Steroid ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Adrenalectomized Rats

Lyophilized granuloma pouch exudates obtained from intact or adrenalectomized rats contain an anti-inflammatory substance active parenterally in the cotton pellet and in the granuloma pouch methods. In these crude preparations the activity is only a small fraction of that of cortisone acetate. Both exudates are active in hypophysectomized animals but not in adrenalectomized animals. It appears that the active fraction is not itself an adrenal steroid, but it is possible that small amounts of adrenal hormones act as conditioning factors for the anti-inflammatory activity. The release of a nonadrenal anti-inflammatory substance during inflammation would help explain some anergic phenomena; such a substance could also be involved in limiting and terminating the inflammatory process.

scholarly journals Association between Abscess Fluid and Procalcitonin in Blood Serum

2020 ◽  
Vol 13 (2) ◽  
pp. 109-115
Author(s):  
Nadezhda Anatol'evna Zubareva ◽  
Dmitriy Yur'evich Sosnin ◽  
Andrey Viktorovich Renzhin
Keyword(s):  
Blood Serum ◽  
Organ Failure ◽  
Soft Tissues ◽  
Comparison Group ◽  
Statistical Significance ◽  
Abdominal Cavity ◽  
Test System ◽  
Control Group ◽  
Surgical Department ◽  
Experimental Group

Introduction. Currently there are no relevant findings from a comparative study of the chemical composition of the abscess fluid and blood serum.The aim of the research was to study the composition of procalcitonin (PCT) contained in the blood serum and abscess fluids of the soft tissues and the abdominal cavity in local inflammation and sepsis.Materials and methods. The PCT concentration was determined in blood serum and the content of abscesses of the soft tissues and abdominal cavity in 45 patients of the surgical department. The experimental group included 31 patients with inflammatory processes that did not result in the organ failure. The comparison group included 14 patients with abscesses which manifested clinical and laboratory signs of the systemic inflammation and organ failure. The control group included 25 healthy individuals. The PCT level was determined by ELISA using the Procalcitonin - ELISA - BEST test system (Vector - Best, Russia).Results. Differences in the PCT content in the serum between groups were characterized by a high degree of statistical significance (H = 58.79206, p = 0.0000). The highest PCT level in the serum was observed in patients of the comparison group, where the average values ​​were 11.94 4.98 ng / ml, the lowest PCT level in the serum was observed in patients of the control group. The PCT concentration in the content of abscesses was lower compared to blood serum and was characterized by statistically significant differences in patients of both - experimental group and comparison groups (p = 0.00001)Conclusion. Abscesses of the soft tissues and the abdominal cavity without signs of generalized infection are not accompanied by an increase in the PCT concentration in the blood serum for more than 2 ng/ml. The PCT concentration in the content of abscesses was lower than its content in the blood serum in both - the local inflammatory process and in the development of sepsis.

scholarly journals Study of the expression of functionally relative genes in the treatment of coronavirus infection in chickens

2021 ◽  
Vol 51 (5) ◽  
pp. 68-76
Author(s):  
A. S. Bobikova ◽  
V. S. Cherepushkina ◽  
T. E. Mironova ◽  
V. N. Afonyushkin ◽  
N. A. Donchenko ◽  
...  
Keyword(s):  
Integrated Approach ◽  
Antiviral Drugs ◽  
Inflammatory Activity ◽  
Control Group ◽  
Virus Particles ◽  
Infectious Bronchitis ◽  
System A ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Experimental Group

The level of expression of anti-inflammatory cytokines NF-kB, IL-6, IFN-y, Caspasa-3, FC in chickens in the lungs and intestines during the modeling of infectious bronchitis in chickens was studied. To simulate coronavirus pneumonia, the vaccine was administered individually, 10 doses per head orally. The chickens of the 1st experimental group were fed with the Lyumantse preparation at the rate of 3 kg / t of feed, the 2nd experimental group received the Glitsevir drug at the rate of 200 μg / 0.3 ml per head. The chickens of the control group did not receive the preparations. It was revealed that antiviral drugs in the experimental groups suppressed the destruction of epithelial cells in the intestine. This may not always be an indication of a positive character, as in the case of apoptosis, not only the intestinal cells affected by the virus particles but also healthy cells are destroyed. There was a decrease in the number of active macrophages in the intestines of the experimental groups relative to the control. The amount of interferon produced was also below the control, which indicates a decreased activity of the immune system. A higher pro-inflammatory activity in the respiratory system of chickens was detected when Glicevir was used. It consists of increased expression of IL-6, interferon-gamma, macrophage receptor to Fc antibody fragments and inflammatory regulatory factor NF-kB genes compared to Lumantse with anti-inflammatory activity, but also compared to untreated control group chickens. It is concluded that it is possible to predict the risk of an exacerbation of an infectious process in the lungs against the background of a local decrease in the viral load in the intestine. An integrated approach is needed in the treatment of coronavirus infections, including either systemic antiviral drugs or anti-inflammatory drugs.

scholarly journals Status of cellular immunity in rats under conditions of acute widespread petitonitis in the setting of diabetes mellitus

2020 ◽  
Vol 10 (2) ◽  
pp. 5243-5246
Keyword(s):  
Diabetes Mellitus ◽  
Immune Response ◽  
Cellular Immunity ◽  
Abdominal Cavity ◽  
Main Group ◽  
Moderate Increase ◽  
Experimental Animals ◽  
Generalized Peritonitis ◽  
White Rats ◽  
Fecal Suspension

Acute generalized peritonitis (AGP) occurs in patients with concomitant pathology, in particular at diabetes mellitus. The severity of peritonitis depends on the adequacy of the immune response. Purpose of research was to study the features of cellular arm of immune response in the organism of experimental animals with simulated AGP in the setting of diabetes mellitus (DM). 56 white rats were used in the experiment, which was divided into three groups: main group – 24 animals with simulated AWP in the setting of DM; comparison group – 24 animals with simulated peritonitis. Animals of the main group AGP were modelled by injecting 10 % of filtered fecal suspension into the abdominal cavity of tested rats at a dose of 0,5 ml per 100 g of body weight. Removal of material for histological examination was performed on 1, 3 and 7 days. Cellular immunity was determined by a method based on the interaction of fluorescently labeed monoclonal antibodies with lymphocyte surface antigens. In both animals, all indicators of cellular immunity gradually decreased from day 1 to day 7 of the experiment, but these changes were more significant in animals with DM. With AGP, the cellular immune response imbalance is more significant in animals with DM than in animals with isolated AGP, which is characterized by a marked statistically significant decrease in the level of CD3+ cells, CD4+ cells, CD16+ cells, and a moderate increase in CD8+ cells. The imbalance of cellular immunity deepens depending on the duration of the lesion. The level of CD3+ cells at 1, 3, and 7 day in animals with AGP in the setting of DM was 53,77%, 60,48%, and 62,1 %, respectively, lower than the level in the group with animals with AGP. The prolonged imbalance of cellular immunity indices in experimental animals with simulated AGP in the setting of DM indicates not only the occurrence of secondary immunodeficiency, but also significant depletion of the body’s immune forces compared to animals with AGP.

scholarly journals Effects of armadin on hepatic transaminases in rats with acute generalized peritonitis on the background of diabetes mellitus

2021 ◽  
Vol 11 (5) ◽  
pp. 333-338
Author(s):  
M. Regeda ◽  
B. Verveha ◽  
N. Melnyk
Keyword(s):  
Diabetes Mellitus ◽  
Abdominal Cavity ◽  
White Male ◽  
The Body ◽  
Laboratory Animals ◽  
Control Group ◽  
Male Adult ◽  
Generalized Peritonitis ◽  
Streptozotocin Induced Diabetes ◽  
Experimental Group

The article discusses the issues about the effect of the Armadin on the hepatic transaminase in rats with acute generalized peritonitis on the background of diabetes mellitus. The experiment was performed on 48 white male adult rats, which were divided into two groups. The experimental group – animals with simulated acute generalized peritonitis against the background of streptozotocin-induced diabetes with the drug “Armadin” (ethylmethylhydroxypyridine succinate) application (n=24). The control group – animals with simulated acute generalized peritonitis against the background of streptozotocin-induced diabetes without pharmacocorrection (n = 24). Diabetes mellitus was simulated by a single intraperitoneal injection of streptozotocin at the dose of 60 mg/kg. Acute generalized peritonitis was induced with 10% faecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method. Every day for 6 days, rats of experimental group received the drug “Armadin” at the doses of 100 mg/kg. We found a probable decrease in aminotransferase activity on day 3 of the study in rats of the experimental group after administration of Armadin. Thus, ALT activity decreased by 26.7%, and AST activity – by 25.9% compared to the control group. On the 7th day of the study, the activity of ALT in the blood of rats of the experimental group decreased to 0.50 ± 0.03 mmol/l, which was 49.5% lower than the control group of rats in this period of the experiment. Similar changes are observed in the study of AST activity, where, accordingly, it decreased by 48 % relative to control. The positive effect of the drug "Armadin" on the body of rats, with AGP on the background of STZ-induced diabetes, is manifested by the restoration of the functional state of the liver.

Benoxaprofen (opren)

1981 ◽  
Vol 19 (24) ◽  
pp. 95-96
Keyword(s):  
Mode Of Action ◽  
Inflammatory Process ◽  
Acute Inflammation ◽  
Inflammatory Activity ◽  
Inflammatory Drug ◽  
Anti Inflammatory ◽  
Animal Tests

Benoxaprofen (Opren - Dista) is a non-steroidal anti-inflammatory drug (NSAID) with a novel mode of action. Other NSAIDs inhibit the formation of prostaglandins which are important in acute inflammation. Benoxaprofen has little such activity, but modifies leucocyte function, especially the migration of monocytes which perpetuate the inflammatory process.1 The significance of this mode of action is unclear. In the usual animal tests of anti-inflammatory activity benoxaprofen is as effective as phenylbutazone.2

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