scholarly journals Age Dependency of Spermatogenesis Efficiency in Cats

2020 ◽  
Vol 9 (1) ◽  
pp. 64-68
Author(s):  
P. M. Torgun ◽  
D. B. Nikityuk ◽  
S. V. Klochkova ◽  
N. T. Alexeeva ◽  
A. G. Kvaratskheliya ◽  
...  

The purpose of this study is to study the efficiency of spermatogenesis in old and young cats in various seasons of the yearMaterial and methods. The material has been collected from 16 cats of different ages in winter and summer periods in Voronezh veterinary clinics while animals being sterilized. The testicles were fixed in Shtiva’s liquid and Buen’s liquid. The material was poured into paraffin and a series of paraffin sections 4–5 μm thick were prepared. Sections were stained with hematoxylin and eosin, iron hematoxylin, Heidengain azane, trichrome-PAS reaction and tetrachrome-PAS reaction. By means of a helical eyepiece-micrometer, the diameter of the testicles tubules and the epididymis (50 measurements for each animal) were measured. To determine the effectiveness of spermatogenesis at an increase in 900 times, the number of first-order spermatocytes in the zigotene and pachitene stage, early spermatid (50 canals for each animal) was estimated. The normality of the distribution was determined using the Kolmogorov–Smirnov test and the Lilliefors adjustment. The measurement results were processed using the nonparametric Mann–Whitney U-criteria. Changes at Р<0.05 were considered statistically significant.Results. The maximum diameter of the testicular tubules and the canal of the epididymis was found in animals at two years of age. These parameters in cats at the age of 8 years are reduced by 29.2%, and by17.0%, respectively. Comparing the number of dying cells in old and young animals, it can be noted that in cats at the age of 8 years the number of dying spermatids increases, while spermatogenesis efficiency decrease is statistically significant (by 19.1%).

FLORESTA ◽  
2012 ◽  
Vol 42 (4) ◽  
pp. 741 ◽  
Author(s):  
Saulo Jorge Téo ◽  
Júlio César Bianchi ◽  
Adriano Peloso ◽  
Paulo Roberto Nava ◽  
Alan Marcon ◽  
...  

ResumoO objetivo deste trabalho foi analisar as funções de densidade probabilísticas (FDP) Normal, Ln-Normal, Sb de Johnson, Weibull 3P, Gamma, Beta e Weber, para descrever as mudanças na estrutura diamétrica de povoamentos de Pinus taeda L., na região de Caçador (SC), em diferentes idades e classes de sítio. O processamento dos dados foi realizado por meio da ferramenta Solver, do software MS Excel 2010, a qual utiliza o algoritmo linear de gradiente reduzido generalizado (GRG) na interação dos parâmetros. Verificou-se que a FDP Sb de Johnson e Weibull 3 P apresentaram os melhores desempenhos. Para a avaliação da aderência das FDP, é recomendada a utilização das estatísticas R2, R2aj, syx e syx%, além do teste de Kolmogorov-Smirnov, em todos os casos, especialmente quando houver número de observações superior a 5.000. Geralmente, houve um aumento da amplitude dos valores dos diâmetros e um achatamento da distribuição diamétrica com o avanço da idade e com a melhora da produtividade do sítio. Com o progresso da idade, há um aumento dos valores do diâmetro máximo e do diâmetro modal das distribuições, para as classes de sítio de maior produtividade, porém o mesmo não ocorre para o sítio menos produtivo. AbstractPerformance of probability density functions in order to describe diameter distribution of Pinus taeda, in the region of Caçador, SC. This research aims to analyze probability of density functions (pdf) Normal, Ln-Normal, Johnson Sb, 3 P Weibull, Gamma, Beta and Weber in order to describe  diameter changes in Pinus taeda L. plantations structure, in the region of Caçador - SC, Brazil, at different age and site classes. The data processing was carried out by Solver tool of the software MSExcel2010, using the linear algorithm of generalized reduced gradient (GRG) for interaction of parameters. As result, Johnson Sb and 3PWeibull presented the best performances. For the pdf adherence evaluation, it was recommended the employment of R2, R2aj, syx e syx% statistics, besides the Kolmogorov-Smirnov test, in any situation, specially, when there is more than 5,000 observations. Generally, there was an increasing in the range of diameter values and a flatness of diameter distribution at advancing age and improvement of the site productivity. At age advancing, there was an increasing of maximum diameter and modal diameter values of distributions, for the higher productivity site classes, on the other hand, the same did not occur for the low productivity site class.Keywords: Horizontal structure; Kolmogorov-Smirnov test; probability density function; forestry site.


2021 ◽  
Vol 10 (2) ◽  
pp. 43-48
Author(s):  
A. G. Ul'yanov ◽  
N. A. Slesarenko ◽  
N. T. Alekseeva ◽  
P. M. Torgun ◽  
A. G. Kvaratskheliiya

The aimof this study is to study the recovery processes in the thyroid gland of Russian long-haired sheep in postnatal ontogenesis.Material  and  methods. The  material  for  the  study  was  77  rams  of  the  Russian  long-haired  breed  of various ages. Fragments of the thyroid gland were fixed in a 10% solution of neutral formalin, in liquids of Shtive and Buena. Thin paraffin sections with a thickness of 4–5 μm were stained with hematoxylin and eosin, azan according to Heydenhein, and the PAS-reaction was applied. The average area of all follicles in the field of view was determined at a magnification of ×80 (ob. 10; oc. 8) using G.G. Autandilov ocular grid (50 fields of viewfor each animal). Based on the obtained absolute values of the area of the follicles and the interfollicular epithelium (mm 2 ), the relative value (%) of the area of the follicles and the interfollicular epithelium was determined. Using a screw eyepiece micrometer, the diameter of the nuclei of thyrocytes in the follicles and thyrocytes of the interfollicular epithelium was measured. The area of the nuclei was determined by the diameter of the nuclei. The mitotic index (MI) in ‰ was calculated . The number of dividing cells per thousand thyrocytes was calculated. At  least 20,000 cells were counted for each animal.Results.It was found that in rams aged 15 days, the area of interfollicular epithelium was 86.5%, microfollicles occupied an area of 13.5%. Dividing cells were common, with the MI of follicular and interfollicular thyrocytes being 0.19‰. In rams at the age of 2 months, the number of small follicles increased, the areaof which was 19.7%. MI in follicles increased to 0.21‰, in interfollicular epithelium it decreased to 0.16‰. In rams at the age of 4 months, the area of follicles almost doubled, the area of interfollicular epithelium decreased by 16.1%. In rams aged 2–3 years, the area of interfollicular epithelium decreased, while MI decreased and was 0.13‰ in 3-year-old animals. In rams at the age of 4 and 5 years, there was a decrease in the functional activity of thefollicles. MI in large follicles was higher than in small follicles (0.12–0.13‰ versus 0.09‰).


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohammadreza Azimi ◽  
Seyed Ahmad Rasoulinejad ◽  
Andrzej Pacut

AbstractIn this paper, we attempt to answer the questions whether iris recognition task under the influence of diabetes would be more difficult and whether the effects of diabetes and individuals’ age are uncorrelated. We hypothesized that the health condition of volunteers plays an important role in the performance of the iris recognition system. To confirm the obtained results, we reported the distribution of usable area in each subgroup to have a more comprehensive analysis of diabetes effects. There is no conducted study to investigate for which age group (young or old) the diabetes effect is more acute on the biometric results. For this purpose, we created a new database containing 1,906 samples from 509 eyes. We applied the weighted adaptive Hough ellipsopolar transform technique and contrast-adjusted Hough transform for segmentation of iris texture, along with three different encoding algorithms. To test the hypothesis related to physiological aging effect, Welches’s t-test and Kolmogorov–Smirnov test have been used to study the age-dependency of diabetes mellitus influence on the reliability of our chosen iris recognition system. Our results give some general hints related to age effect on performance of biometric systems for people with diabetes.


Author(s):  
Du Wenliao ◽  
Guo Zhiqiang ◽  
Gong Xiaoyun ◽  
Xie Guizhong ◽  
Wang Liangwen ◽  
...  

A novel multifractal detrended fluctuation analysis based on improved empirical mode decomposition for the non-linear and non-stationary vibration signal of machinery is proposed. As the intrinsic mode functions selection and Kolmogorov–Smirnov test are utilized in the detrending procedure, the present approach is quite available for contaminated data sets. The intrinsic mode functions selection is employed to deal with the undesired intrinsic mode functions named pseudocomponents, and the two-sample Kolmogorov–Smirnov test works on each intrinsic mode function and Gaussian noise to detect the noise-like intrinsic mode functions. The proposed method is adaptive to the signal and weakens the effect of noise, which makes this approach work well for vibration signals collected from poor working conditions. We assess the performance of the proposed procedure through the classic multiplicative cascading process. For the pure simulation signal, our results agree with the theoretical results, and for the contaminated time series, the proposed method outperforms the traditional multifractal detrended fluctuation analysis methods. In addition, we analyze the vibration signals of rolling bearing with different fault types, and the presence of multifractality is confirmed.


Revista CEFAC ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 37-43
Author(s):  
Rafaela Coelho Minsky ◽  
Tayná Castilho ◽  
Roseane Rebelo Silva Meira ◽  
Tatiana Godoy Bobbio ◽  
Camila Isabel Santos Schivinski

ABSTRACT Purpose: to analyze whether deleterious oral habits can influence the number of attempts of forced spirometry maneuvers performed by healthy children. Methods: this observational and cross-sectional analytical study included 149 healthy children aged 6-12 years attending public and private schools in Florianópolis, SC, Brazil. A validated protocol was applied for the analysis of deleterious oral habits. The children were grouped according to the number of spirometry maneuvers needed to achieve successful spirometry results, as follows: G1) children who needed 3 maneuvers; G2) 4 maneuvers; G3) 5-8 maneuvers. Data were analyzed with the Kolmogorov-Smirnov test and the Kruskal-Wallis test was applied to compare quantitative variables between the groups. The Chi-square test was used to assess the association between the groups and qualitative variables. Results: there was no association between the number of attempts and the qualitative variables evaluated by the protocol. There was also no difference between the groups regarding quantitative variables for breastfeeding time, breastfeeding occurrence, use of pacifiers, and thumb sucking. Conclusion: the presence of DOH did not influence the number of forced spirometry maneuvers, performed by the healthy children in this study.


2021 ◽  
Vol 15 (7) ◽  
pp. 1940-1944
Author(s):  
Sevcan Altun ◽  
Aykut Aksu ◽  
Osman Imamoglu ◽  
Murat Erdogdu ◽  
Kursat Karacabey

The aim of this study is to investigate the nutritional approaches of student athletes studying at the university during the coronavirus outbreak period. Participants consisted of students studying and doing sports at the University. 446 students, 246 males and 200 females, participated in the study. Besides the personal form, students were filled the questionnaire testing questionnaire. Students voluntarily participated. The surveys were done on social media. Nutritional habits questionnaire consists of 12 questions. In the preparation of the survey questions, the questions proved validity of the researches which have been done on the subject before have been used. SPSS 23.00 package program was used in statistical analyses. Kolmogorov-Smirnov test was performed to test whether the data was normally distributed and it was determined that the data showed normal distribution. Independent t-test, paired t-test, unidirectional variance analysis and LSD tests were used in statistical operations. There was no significant difference in students' nutrition approaches by gender, both in the pre-outbreak period and in the outbreak period points (p> 0.05). Nutrition scores were significantly increased during the outbreak period (p <0.001). A significant difference was found between the students who felt bad before the epidemic and those who felt well before the epidemic and their nutritional scores according to the levels they felt (p <0.05). A significant difference was found between the pre-outbreak period and post-epidemic nutrition scores of the sports faculty students (p <0.05). During the coronavirus epidemic, university student athletes have either increased their nutritional opportunities or have changed their eating habits positively to keep their immune systems strong or both. The fact that sports faculty students have better nutrition compared to other faculty students can be attributed to their taking courses in nutrition, health and similar. It is recommended to give lectures or seminars on nutrition to athlete students. Keywords: Student, Nutrition, Sports Nutrition, Nutritional Approach, Covid-19


2017 ◽  
Vol 78 (2) ◽  
pp. 328-336
Author(s):  
M. S. C. S. Lima ◽  
J. Pederassi ◽  
C. A. S. Souza

Abstract The practice of capture-recapture to estimate the diversity is well known to many animal groups, however this practice in the larval phase of anuran amphibians is incipient. We aimed at evaluating the Lincoln estimator, Venn diagram and Bayes theorem in the inference of population size of a larval phase anurocenose from lotic environment. The adherence of results was evaluated using the Kolmogorov-Smirnov test. The marking of tadpoles for later recapture and methods measurement was made with eosin methylene blue. When comparing the results of Lincoln-Petersen estimator corresponding to the Venn diagram and Bayes theorem, we detected percentage differences per sampling, i.e., the proportion of sampled anuran genera is kept among the three methods, although the values are numerically different. By submitting these results to the Kolmogorov-Smirnov test we have found no significant differences. Therefore, no matter the estimator, the measured value is adherent and estimates the total population. Together with the marking methodology, which did not change the behavior of tadpoles, the present study helps to fill the need of more studies on larval phase of amphibians in Brazil, especially in semi-arid northeast.


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