Identification of Fasciola Species Using Tegumental Spines in Tissue Sections

2020 ◽  
Author(s):  
Arezoo FADAVI ◽  
Keyhan ASHRAFI ◽  
Hamid HASSANPOUR ◽  
Mohamad Bagher ROKNI ◽  
Seyyed Mostafa HOSSEINI ◽  
...  
Keyword(s):  
Staining Technique ◽  
Morphological Characters ◽  
Image Analysis System ◽  
Scanning Electron Micrographs ◽  
Tissue Sections ◽  
Fasciola Species ◽  
Accuracy Of Measurement ◽  
Hematoxylin And Eosin ◽  
Analysis System ◽  
Liver Flukes

Background: Efforts to find a reliable non-molecular means of identification has been the main purpose of the current work that always is persuaded by researchers interested in the field of parasitology. Methods: Adult fasciolids were obtained from the slaughterhouses in different parts of Iran in 2017, and investigated using the classical old fashion morphological appearances of the worms implementing a camera lucida equipped microscope. Histological procedure was subsequently performed for almost the entire collected adult worms followed by Hematoxylin and Eosin (H&E) staining technique. DNA extraction and RFLP-PCR technique were carried out for the entire fasciolid liver flukes. To attain more comparable morphological conclusions, Scanning Electron Micrographs were also implemented for two molecularly identified fasciolids. Results: Based on spine morphology observed in worm’s tissue sections two types of tegumental spines, “pointed” and “molar” shapes have been identified addressing to distinguish F. hepatica and F. gigantica species respectively. The present identification has been also supported by Molecular analysis using RFLP-PCR technique. Conclusion: There are some hidden morphological characters implemented in species identification for certain helminths. Meanwhile, the emergence of computer image analysis system (CIAS) on the scene of taxonomy, has revolutionized the accuracy of measurement in morphology by employing detailed parameters that have not been regarded before. The current study has illustrated the tegumental spines of two Fasciola species in tissue sections which has not been enough considered in helminthological publications so far.

scholarly journals Histologic study of use of microfibrillar collagen hemostat in rat dental sockets

2003 ◽  
Vol 14 (1) ◽  
pp. 12-15 ◽  
Author(s):  
Natasha Magro-Érnica ◽  
Osvaldo Magro-Filho ◽  
Idelmo Rangel-Garcia
Keyword(s):  
Control Group ◽  
Image Analysis System ◽  
Bone Area ◽  
Central Incisor ◽  
Histologic Study ◽  
Socket Healing ◽  
Hematoxylin And Eosin ◽  
Analysis System ◽  
The Right ◽  
Qualitative Analyses

The aim of this paper was to evaluate if the placement of microfibrillar collagen hemostat (MCH) into a dental socket interfered with healing. General anesthesia was administered to 30 adult male Albinus Wistar rats and the maxillary right central incisor was extracted. In the control group after each tooth was extracted, the socket was sutured. In the MCH group after each tooth was extracted, MCH was placed into the socket before suturing. Postoperatively, 5 animals were sacrificed from each group at 7, 21 and 28 days. The right maxilla was removed from each animal and histologic slides were stained with Masson's trichromic and hematoxylin and eosin. Quantitative and qualitative analyses were done. The percentage of bone area in the dental socket was quantified using the Image Lab 98 image analysis system. The bone area formation for the control and MCH groups was: 8.1% and 3.3% at 7 days, 34.4% and 33% at 21 days and 41% and 41.3% at 28 days, respectively. We concluded that MCH interferes with the beginning of dental socket healing but does not interfere with the final healing of the dental socket.

FEATURES OF THE PATHOMORPHOLOGICAL DIAGNOSIS OF MICROCRYSTALLINE ARTHROPATHIES IN THE PRACTICE OF SURGICAL MATERIAL EXAMINATION

2020 ◽  
Vol 58 (3) ◽  
pp. 286-289
Author(s):  
N. S. Migalkin ◽  
T. A. Stupina ◽  
A. V. Kaminsky ◽  
D. S. Mokhovikov ◽  
D. A. Shabalin ◽  
...  
Keyword(s):  
Staining Technique ◽  
Calcium Pyrophosphate ◽  
Alcohol Solution ◽  
Formalin Fixation ◽  
Histological Techniques ◽  
Tissue Sections ◽  
Stereo Microscope ◽  
Hematoxylin And Eosin ◽  
Tissue Specimens ◽  
Msu Crystals

The development of microcrystalline arthritides is most frequently associated with the formation of monosodium urate (MSU) and calcium pyrophosphate (CPP) crystals. Their identification is of crucial importance in recognizing these diseases. Objective: to determine the possibilities of histological techniques in identifying MSU and CPP crystals and to evaluate the effectiveness of the techniques. Subjects and methods. Twenty-four tissue blocks (fragments of the affected areas of the elbow joint, the interphalangeal joint of the index finger, and hip joint) from 7 patients were examined. Paraffin sections were stained with a 0.5% alcohol solution of eosin, as well as with hematoxylin and eosin. Tissue specimens were examined and digitized using an AxioScope.A1 stereo microscope with Zenblue software (Carl Zeiss MicroImaging GmbH, Germany). Results and discussion. When staining the tissue sections with hematoxylin and eosin, microcrystals were not visualized; the major portions of MSU crystals was dissolved during fixation and staining, whereas CPP crystals were masked with hematoxylin as focal basophilic aggregates. The staining technique with an alcohol solution of eosin and short formalin fixation (within 12 hours) made it possible to avoid dissolution of MSU crystals and to visualize both MSU and CPP crystals, and to determine their shape and color. Conclusion. Light microscopy of the tissue sections stained with an alcohol solution of eosin along with short formalin fixation is a reliable method to differentiate MSU and CPP crystals. In patients undergoing endoprosthetic replacement, the significance of this technique for the pathomorphological study of surgical material consists in assessing inflammatory activity and in eliminating a disease, such as microcrystalline arthropathy.

scholarly journals Molecular and Morphological Characterization ofFasciolaspp. Isolated from Different Host Species in a Newly Emerging Focus of Human Fascioliasis in Iran

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Reza Shafiei ◽  
Bahador Sarkari ◽  
Seyed Mahmuod Sadjjadi ◽  
Gholam Reza Mowlavi ◽  
Abdolali Moshfe
Keyword(s):  
Fasciola Hepatica ◽  
Morphological Diversity ◽  
Morphological Characterization ◽  
Image Analysis System ◽  
Human Fascioliasis ◽  
Rflp Profile ◽  
Analysis System ◽  
Liver Flukes ◽  
Southwest Of Iran ◽  
And Control

The current study aimed to find out the morphometric and genotypic divergences of the flukes isolated from different hosts in a newly emerging focus of human fascioliasis in Iran. AdultFasciolaspp. were collected from 34 cattle, 13 sheep, and 11 goats from Kohgiluyeh and Boyer-Ahmad province, southwest of Iran. Genomic DNA was extracted from the flukes and PCR-RFLP was used to characterize the isolates. The ITS1, ITS2, and mitochondrial genes (mtDNA) of NDI and COI from individual liver flukes were amplified and the amplicons were sequenced. Genetic variation within and between the species was evaluated by comparing the sequences. Moreover, morphometric characteristics of flukes were measured through a computer image analysis system. Based on RFLP profile, from the total of 58 isolates, 41 isolates (from cattle, sheep, and goat) were identified asFasciola hepatica, while 17 isolates from cattle were identified asFasciola gigantica. Comparison of the ITS1 and ITS2 sequences showed six and seven single-base substitutions, resulting in segregation of the specimens into two different genotypes. The sequences of COI markers showed seven DNA polymorphic sites forF. hepaticaand 35 DNA polymorphic sites forF. gigantica. Morphological diversity of the two species was observed in linear, ratios, and areas measurements. The findings have implications for studying the population genetics, epidemiology, and control of the disease.

Digital Processing of STEM Images

1981 ◽  
Vol 39 ◽  
pp. 236-237
Author(s):  
A. V. Crewe ◽  
M. Ohtsuki
Keyword(s):  
High Resolution ◽  
Low Dose ◽  
Assembly Language ◽  
Processing System ◽  
Principal Component ◽  
Digital Processing ◽  
Image Analysis System ◽  
Black And White ◽  
Analysis System ◽  
Dose Imaging

We have assembled an image processing system for use with our high resolution STEM for the particular purpose of working with low dose images of biological specimens. The system is quite flexible, however, and can be used for a wide variety of images.The original images are stored on magnetic tape at the microscope using the digitized signals from the detectors. For low dose imaging, these are “first scan” exposures using an automatic montage system. One Nova minicomputer and one tape drive are dedicated to this task.The principal component of the image analysis system is a Lexidata 3400 frame store memory. This memory is arranged in a 640 x 512 x 16 bit configuration. Images are displayed simultaneously on two high resolution monitors, one color and one black and white. Interaction with the memory is obtained using a Nova 4 (32K) computer and a trackball and switch unit provided by Lexidata.The language used is BASIC and uses a variety of assembly language Calls, some provided by Lexidata, but the majority written by students (D. Kopf and N. Townes).

Chemical Classification and Particle Sizing of Foundry Sands

1985 ◽  
Vol 43 ◽  
pp. 388-389
Author(s):  
D.S. DeMiglio
Keyword(s):  
Image Analysis ◽  
Energy Dispersive Spectrometer ◽  
Image Analysis System ◽  
Representative Sampling ◽  
Foundry Sands ◽  
Large Atomic Number ◽  
Backscattered Electron ◽  
Sand Particles ◽  
Analysis System ◽  
Reclamation System

Much progress has been made in recent years towards the development of closed-loop foundry sand reclamation systems. However, virtually all work to date has determined the effectiveness of these systems to remove surface clay and metal oxide scales by a qualitative inspection of a representative sampling of sand particles. In this investigation, particles from a series of foundry sands were sized and chemically classified by a Lemont image analysis system (which was interfaced with an SEM and an X-ray energy dispersive spectrometer) in order to statistically document the effectiveness of a reclamation system developed by The Pangborn Company - a subsidiary of SOHIO.The following samples were submitted: unreclaimed sand; calcined sand; calcined & mechanically scrubbed sand and unused sand. Prior to analysis, each sample was sprinkled onto a carbon mount and coated with an evaporated film of carbon. A backscattered electron photomicrograph of a field of scale-covered particles is shown in Figure 1. Due to a large atomic number difference between sand particles and the carbon mount, the backscattered electron signal was used for image analysis since it had a uniform contrast over the shape of each particle.

Цифровая морфометрия разнокачественности семян овощных культур

2018 ◽  
Author(s):  
F.B. Musaev ◽  
N.S. Priyatkin ◽  
M.V. Arkhipov ◽  
P.A. Shchukina ◽  
A.F. Bukharov ◽  
...  
Keyword(s):  
Seed Quality ◽  
Image Analysis System ◽  
Flatbed Scanner ◽  
The Future ◽  
Scanned Images ◽  
Computer Morphometry ◽  
Analysis System ◽  
Matrix Heterogeneity ◽  
Relationship Of ◽  
Selection Of

Приведено описание разработанной авторами методики цифровой компьютерной морфометрии семян овощных культур на основе системы анализа изображений, состоящей из планшетного сканера и программного обеспечения для автоматических измерений. В основу метода положено представление о разнокачественности семян, обусловленной генетической неоднородностью самих семенных растений, используемых в промышленном семеноводстве. Физические свойства семян (их форма и линейные размеры) – основные параметры при определении их качества. Цифровые изображения семян получены при помощи планшетного сканера HP Sсanjet 200 на базе Агрофизического НИИ с использованием серийного программного обеспечения «Argus-BIO», производства ООО «АргусСофт» (г. Санкт-Петербург). Метод состоит из подбора контрастной подложки (фона) для сканирования семян с минимальными теневыми эффектами, калибровку программного обеспечения для привязки к истинным размерным величинам, подбор параметров измерений и автоматическое распознавание цифровых сканированных изображений семян. Представлены экспериментальные данные по морфометрии экологически разнокачественных семян фасоли овощной, матрикально разнокачественных семян укропа, пастернака и лука Кристофа. Семена укропа и пастернака, собранные из разных порядков ветвления семенного растения, значительно различались по величине линейных параметров. Наиболее показательный линейный параметр семян – площадь проекции. Предложенная авторами методика цифровой морфометрии, уже использована на практике и в перспективе может быть задействована в исследованиях экологической и матрикальной разнокачественности семян овощных культур. Так, она прошла апробацию на разнокачественных семенах пяти сортов фасоли овощной (Настена, Магура, Миробела, Морена, Бажена) полученных в пяти контрастных эколого-географических условиях среды (Москва, Белгород, Ставрополь, Омск, Горки) в 2011–2012 годах. В дальнейшем методика может быть использована для улучшения качества цифровых изображений семян, изучения разнокачественности семян в том числе и для совершенствования контроля за селекционным процессом. Кроме того, она применима для изучения взаимосвязи совокупности морфометрических характеристик семян и их посевных качеств.The description of the method of digital computer morphometry of vegetable seeds developed by the authors on the basis of the image analysis system consisting of a flatbed scanner and software for automatic measurements is given. The method is based on the idea of seed quality, due to the genetic heterogeneity of the seed plants used in industrial seed production. Physical properties of seeds (their shape and linear dimensions) are the main parameters in determining their quality. Digital image of the seed obtained using the flatbed scanner, HP Sсanjet 200 on the basis of the Agrophysical research Institute with serial software “Argus-BIO”, produced by LLC “Argussoft” (Saint-Petersburg). The method consists of selection of a contrast substrate (background) for scanning seeds with minimal shadow effects, calibration of software for binding to true size values, selection of measurement parameters and automatic recognition of digital scanned images of seeds. Experimental data on the morphometry of ecologically different-quality seeds of vegetable beans, matrix seeds of dill, Pasternak and Christoph onion are presented. Seeds of dill and parsnip, collected from different orders of branching of the seed plant, significantly differed in size of linear parameters. The most revealing linear parameter seed – area projection. The method of digital morphometry proposed by the authors has already been used in practice and in the future can be used in studies of ecological and matrix heterogeneity of vegetable seeds. So, it was tested on different quality seeds of five varieties of vegetable beans (Nastena, Magura, Mirobelа, Morena, Bazhenf) obtained in five contrasting environmental and geographical conditions (Moscow, Belgorod, Stavropol, Omsk, Gorki) in 2011-2012. In the future, the technique can be used to improve the quality of digital images of seeds, study of seed diversity, including to improve the control of the breeding process. In addition, it is applicable to study the relationship of the set of morphometric characteristics of seeds and their sowing qualities.

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