ANTINEPHROPATHY EFFECT OF <i>Aloe vera</i> GEL TO PKC-β LEVEL ON WISTAR RAT KIDNEY IN DIABETES MELLITUS

Aloe veragelhas a large vitamins which has become potential candidate to supressed vascular damaged oforganwhich potentially lead to complications such as diabetic nephropathy. This process through down regulation of PKC-β level as a cascade activator of gene transcription such as contraction and permeability of cell. The research goal was to determinethe effect of Aloe vera gel on PKC-β level in the kidneys of diabetes mellitus (DM) wistar rats. Twenty four rats divided into eight treatments (negative controls, positive controls, Non DM with A.vera gel dosage 30, 60, and 120 mg/day, DM with A.vera gel dosage 30, 60 and 120 mg/day) with three replications each. All of the data was analyzed using one way Anova with Statistical Product and Service Solution 16 software. The research result showed that the A. vera gelhas significant effectto decrease PKC-β level significantly (p < 0,000) in diabetes mellitus (DM) wistar rat kidney. In conclusion A.vera gel supressed diabetic nephropathy process via PKC-β and ROS activity in optimum dosage 30 mg/ day. Keywords: Aloe vera Gel, PKC-β level, Diabetes mellitus.

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EFFECT OF Aloe vera GEL ON SUPEROXIDE DISMUTASE (SOD) LEVEL IN STREPTOZOTOCIN (STZ)-INDUCED DIABETIC WISTAR Rattus novergicus LIVER

KnE Life Sciences ◽

10.18502/kls.v2i1.116 ◽

2015 ◽

Vol 2 (1) ◽

pp. 51

Author(s):

Nia Lukita Ariani ◽

Abdul Gofur ◽

Dwi Listyorini ◽

Hendra Susanto ◽

Yuda Handaya

Keyword(s):

Diabetes Mellitus ◽

Superoxide Dismutase ◽

Aloe Vera ◽

Wistar Rat ◽

Positive Control ◽

Negative Control ◽

Diabetic Rats ◽

Wistar Strain ◽

Aloe Vera Gel ◽

One Way Anova

Diabetes mellitus is a metabolic disorder characterized by the increasing levels of blood glucose as a result of either impaired of insulin secretion, insulin action, or both. Persistent hyperglycemia causes oxidative stress. Aloe vera is known of having an antioxidant activity. One of intrinsic antioxidant enzyme is superoxide dismutase (SOD) which eliminates superoxide radical. This research aims to determine the effect of Aloe vera gel on SOD level in STZ-induced diabetic Wistar rat liver. Wistar strain of Rattus novergicus used in this research were grouped into eight groups, consist of negative control (K-), non diabetic groups (NDM) consist of NDM 1 (dose of 30 mg/day), NDM 2 (dose of 60 mg/day), NDM 3 (dose of 120 mg/day), positive control (DM), and the DM group consists of DM 1 (dose of 30 mg/day), DM 2 (dose of 60 mg/day), and DM 3 (dose of 120 mg/day), with three replications each. Diabetic rats were induced using an intra-peritoneal injection of 60 mg/kg BW STZ. Three milliliters of Aloe vera gel were given intragastrointestinally for 14 days started from three days after injection of STZ. The level of liver SOD was measured with spectrophotometer.The results of One-Way Anova showed that Aloe vera gel has a significant effect on SOD levels (p< 0.05). Further analysis using LSD showed that only the treatment of NDM with 60 mg/day of Aloe vera gel giving a significant decreasing of SOD level compared to (K-). The conclusion is that Aloe vera has potency as a natural antioxidant. Keywords: Aloe vera, diabetes mellitus, superoxide dismutase (SOD), liver, streptozotocin (STZ)

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The role of nicotinamide in the correction of renal function in diabetic nephropathy

Reports of Vinnytsia National Medical University ◽

10.31393/reports-vnmedical-2019-23(2)-06 ◽

2019 ◽

Vol 23 (2) ◽

pp. 218-221

Author(s):

L. V. Yanitskaya ◽

L. F. Osinskaya ◽

A. V. Redko

Keyword(s):

Diabetes Mellitus ◽

Body Weight ◽

Diabetic Nephropathy ◽

Statistical Analysis ◽

Rat Kidney ◽

Clinical Manifestations ◽

Diabetic Rats ◽

Cortical Layer ◽

The Difference

Hyperglycemia of diabetes mellitus leads to the activation of the polyol way of oxidation of glucose with the activation of the enzymes of aldose reductase and sorbitol dehydrogenase and of their coenzymes NADPH and NAD, which triggers the mechanism of formation of sorbitol. The consequences of these changes lead to microangiopathy of the tissues of the kidneys, which may be one of the pathogenetic mechanisms of diabetic nephropathy. In an accessible literature, the role of coenzymes of sorbitol pathway in the development of diabetic nephropathy is not sufficiently defined. The purpose of the study was to study the content of NAD and NADPH coenzymes, their correlation, and their role in the mechanism of kidney damage in diabetes mellitus and to predict the possible correction of these changes with the NAD-nicotinamide derivative. The study was conducted on a model of streptotrozectinic diabetes mellitus (single administration of streptozotocin in a dose of 60 mg per 1 kg of body weight). Four weeks after induction of diabetes, nicotinamide (100 mg per 1 kg body weight) was injected. The level of glucose was determined by the Accu-chek (Roshe Diagnostics, Switzerland) glucose meter. The content of NAD and NADH was determined in the non-protein extracts. The statistical analysis was carried out using the Microsoft Excel statistical analysis program. The difference between the indicators was considered statistically significant (p<0.05). The NAD level was reduced by 31%, the NAD/NADN ratio was 32%. The dependence of the ratio of NADP/NADPN in conditions of hyperglycemia of diabetes mellitus with clinical manifestations of diabetic nephropathy is determined. A decrease in the ratio of NADP/NADPN to 38% in the rat kidney in the cortical layer was detected. The introduction of nicotinamide normalized the reduced content of NAD diabetic rats. These results provide perspectives for further research in which nicotinamide can be used as a renal protector.

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Hypoglycemic and hypolipidemic effects of processed Aloe vera gel in a mouse model of non-insulin-dependent diabetes mellitus

Phytomedicine ◽

10.1016/j.phymed.2009.02.014 ◽

2009 ◽

Vol 16 (9) ◽

pp. 856-863 ◽

Cited By ~ 64

Author(s):

Kwanghee Kim ◽

Hyunyul Kim ◽

Jeunghak Kwon ◽

Sungwon Lee ◽

Hyunseok Kong ◽

...

Keyword(s):

Diabetes Mellitus ◽

Mouse Model ◽

Aloe Vera ◽

Insulin Dependent Diabetes Mellitus ◽

Insulin Dependent Diabetes ◽

Dependent Diabetes Mellitus ◽

Aloe Vera Gel ◽

Insulin Dependent

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A Study to assess the Effectiveness of Aloe Vera Gel Dressing on Wound Status among Patients with Diabetes Mellitus in Government Hospital, Dindigul

International Journal of Advances in Nursing Management ◽

10.5958/2454-2652.2019.00038.6 ◽

2019 ◽

Vol 7 (2) ◽

pp. 159

Author(s):

T. Linda Daphne ◽

Vijayarani Prince

Keyword(s):

Diabetes Mellitus ◽

Aloe Vera ◽

Government Hospital ◽

Aloe Vera Gel ◽

Patients With Diabetes

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Hypoglycemic and Hepatoprotective Effects of Processed Aloe vera Gel in a Mice Model of Alloxan Induced Diabetes Mellitus

Journal of Diabetes & Metabolism ◽

10.4172/2155-6156.1000303 ◽

2013 ◽

Vol 04 (09) ◽

Cited By ~ 1

Author(s):

Bhaskar Sharma Sufiyan Siddiqui

Keyword(s):

Diabetes Mellitus ◽

Aloe Vera ◽

Mice Model ◽

Aloe Vera Gel ◽

Hepatoprotective Effects

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Effect of Sphagneticola Trilobata Extract on Histological Wistar Rat Kidney Induced by DMBA

International Journal of Engineering, Science and Information Technology ◽

10.52088/ijesty.v1i3.82 ◽

2021 ◽

Vol 1 (3) ◽

pp. 28-34

Author(s):

Bima Juanda Surbakti ◽

Vivi Mardina ◽

Beni Al Fajar

Keyword(s):

Treatment Group ◽

Rat Kidney ◽

Wistar Rat ◽

Negative Control ◽

Female Rats ◽

Optimum Dose ◽

Treatment Groups ◽

Randomized Design ◽

Negative Controls

One factor can trigger the growth and development of cancer cells is free radicals that are from carcinogenic compounds such as dimethylbenz (α) anthracene (DMBA). The use of plant extracts as a preventive or curative in cases of tumors/cancer has been reported, however there is no reports about the in vivo study for Sphagneticola trilobata plant. S. trilobata is an herbal plant that has pharmacological activities, potential to be developed as anticancer agent. This study aims to examine the anticancer effect of the methanol extract of S. trilobata leaves using histology observation on Wistar rat (Rattus novergicus) kidney which was induced by 7,12 dimethylbenz [α] anthracene (DMBA). The study used a completely randomized design with female rats (15 rats) grouped into 5 treatment groups, namely (i) the normal treatment group (KN), (ii) the DMBA-only treatment group (negative control, K(-)), (iii) the first dose (200 mg / kg BW) treatment group (KI), (iv) the second dose (300 mg / kg BW) treatment group (KII), and (v) the third dose (400 mg / kg BW) treatment group (KIII). DMBA was given orally at a concentration of 18 mg / kg BW for 4 times then continued for the extract. The results showed that cell damages (degeneration, necrosis and inflammation) were found mostly in negative controls. The dosage of 200 mg / kg BW of S. trilobata extract was the optimum dose in this study which was able to inhibit histological damage of kidney organs exposed to carcinogens DMBA by decreasing the level of degeneration, necrosis and inflammation.

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