scholarly journals Effects of Different Extraction Methods and Solvents on the Phenolic Composition and Antioxidant Activity of Silybum Marianum Leaves Extracts

2018 ◽  
Vol 5 (3) ◽  
pp. 3641-3647 ◽  
Author(s):  
Abbassia Benchaachoua ◽  
Hassiba Mahdjoub Bessam ◽  
Imene Saidi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Condensed Tannin ◽  
Ethanolic Extract ◽  
Extraction Methods ◽  
Aqueous Methanol ◽  
Silybum Marianum ◽  
Antioxidant Power ◽  
Extraction Solvent ◽  
Percentage Yield

Objective: The objectives of this study were essentially to evaluate the best technique for extracting phenolic compounds from Silybum marianum leaves. Subjects and Methods: Two extraction methods (maceration and decoction), using four extraction solvents. The efficiency of the extraction methods was estimated by quantifying the total polyphenol contents by the Folin -Ciocalteu method and by determining the antioxidant power of the various extracts by the ability to trap the free radical DPPH °, and the influence of these parameters on the antioxidant activity. Results: The results showed that the extracts of the leaves by maceration have contained the highest percentage yield (24.68%); the highest content in the aqueous -methanol polyphenols extract (18.75 ± 0.55 mg GAE / g DW ) against the decoction is more effective for the extraction of flavonoids and condensed tannins. The highest content of flavonoids had been recorded for the aqueous- ethanolic extract (5.08 ± 0.57 CE mg / g DW). The water had the highest content of condensed tannin (03.86 ± 0.22 TAE / g DW). On the other hand, the aqueous -ethanolic and aqueous -methanol extract of the leaves obtained by maceration have the best antioxidant capacity (73.84 ± 5.90 EAG / g DW and 73.64 ± 5.93 mg EAG / g DW respectively) and the aqueous extract of the leaves by decoction had a better antiradical power (48.64 μg / ml). Conclusion: The extraction of phenolic compounds from Silybum marianum leaves was strongly influenced by the type of solvent and the extraction method. In addition, it is very difficult to choose an appropriate extraction solvent for all plant samples.

scholarly journals Phenolic Profile and Antioxidant Activity of Propolis Extracts From Poland

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984977 ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Agnieszka Waśkiewicz ◽  
Tomasz Rogoziński ◽  
Izabela Ratajczak
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Natural Material ◽  
Antioxidant Power ◽  
Antioxidant Agents

Propolis is a natural material collected by honeybees, containing bioactive compounds that exhibit biological activity. The aim of this study was to assess the chemical composition of Polish propolis extracted with two different concentrations of ethanol, namely 70% and 96%, and to evaluate their antioxidant activity depending on extraction conditions. Samples of Polish propolis were extracted with 70% and 96% ethanol in order to obtain the ethanolic propolis extracts EEP70 and EEP96, respectively. Concentrations of 10 flavonoids and 6 phenolic acids were determined using the UPLC-PDA-TQD system. The antioxidant properties were determined based on the DPPH· free radical scavenging activity, Fe3+ reducing power assay, and ferrous ions (Fe2+) chelating activity assays. Moreover, the effects of the propolis extracts on human red blood cell morphology , the selective permeability of their membrane, as well as on free radicals-induced hemolysis were also assessed. Qualitative and quantitative analyses of both propolis extracts indicated that 70% ethanolic extract contained higher amounts of phenolic compounds than 96% ethanolic extract. The levels of antioxidant activity indicated that both Polish propolis extracts exhibited a high and comparable antioxidant power. The concentration of ethanol used for extraction had no effect on the antioxidant potential of propolis. The presented results indicate that the extracts of Polish propolis are rich in phenolic compounds and are very effective as antioxidant agents. Therefore, they may be applied as a constituent of products used in phytotherapy regardless of the concentration of ethanol used in propolis extraction.

scholarly journals Comparative Evaluation of Argania spinosa and Olea europaea Leaf Phenolic Compounds and their Antioxidant Activity

Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Total Flavonoids Content

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.

scholarly journals Total phenolics, flavonoids, tannins and antioxidant activity of lima beans conserved in a Brazilian Genebank

2014 ◽  
Vol 45 (2) ◽  
pp. 335-341 ◽  
Author(s):  
Tânia da Silveira Agostini-Costa ◽  
Ana Flávia Pádua Teodoro ◽  
Rosa de Belem das Neves Alves ◽  
Leandro Ribeiro Braga ◽  
Ieler Ferreira Ribeiro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Abiotic Factors ◽  
Condensed Tannin ◽  
Germplasm Collection ◽  
Lima Bean ◽  
Ex Situ ◽  
Phaseolus Lunatus ◽  
Beneficial Effects ◽  
Lima Beans

The objective of this study was to characterize for the first time polyphenols and DPPH (2-diphenyl-1-picryhydrazyl radical) antioxidant activity in commonly cultivated accessions of Phaseolus lunatus from an ex situ germplasm collection maintained by Embrapa, in Brazil. Furthermore, the study aimed to detect changes in total polyphenols, total flavonoids and condensed tannin for the same accessions after regeneration in a greenhouse. The results showed the diversity of the lima bean collection for phenolic compounds, which were strongly correlated with antioxidant activity. Lima beans accessions with the highest polyphenols and antioxidant activity were those with colored seeds. Conservation through cold storage of P. lunatus seeds in a cold chamber in the germplasm collection did not necessarily affect phenolic compounds. Variations observed in values after regeneration seeds may be mainly results of biotic and abiotic factors, including not only cultivar, but also environmental conditions. This study suggests that polyphenols in the lima beans present antioxidant activity, with possible beneficial effects for human health. It was expected that the potential of this tasty legume can be also used as a functional food crop and/or as a new ingredient in gastronomy.

Genotypic variation for phenolic compounds in developing and whole seeds, and storage conditions influence visual seed quality of yellow dry bean genotypes

2020 ◽  
Vol 100 (3) ◽  
pp. 284-295
Author(s):  
Mei Xiong ◽  
Mengli Zhao ◽  
Zhen-Xiang Lu ◽  
Parthiba Balasubramanian
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Seed Coat ◽  
Storage Temperature ◽  
Condensed Tannin ◽  
Consumer Preference ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Duration Of Storage

Seed coat colour is an important determinant of the visual quality of dry beans, as seeds are sold as a dry commodity. Phenolic compounds have a major effect on the colour of bean seeds. The objectives of the study were to determine the changes in phenolic compounds during seed development and in whole seeds of yellow bean genotypes with contrasting seed coat colour, and the effects of storage temperature and duration on seed phenolics and colour. Condensed tannin, phenolic acid, flavonoids, and antioxidant activity were observed as early as 10 d after flowering in the developing seeds of Arikara Yellow, which darken at harvest and during postharvest storage. In contrast, for CDC Sol and AAC Y073 seeds which remain yellow, phenolic compounds and antioxidant activity were consistently low. Seed brightness (L*) and yellow colour (b*) were negatively correlated with phenolic compounds and antioxidant activity, and conversely seed redness (a*) was positively correlated with phenolic compounds, confirming a negative influence of phenolic compounds on seed coat colour. Yellow bean genotypes had low anthocyanin but were high in β-carotene. Storage temperature influenced condensed tannin and seed coat colour, whereas the duration of storage influenced phenolic compounds, antioxidant activity, and seed coat colour. Higher temperatures (20 or 30 °C) and longer storage duration (120 or 180 d) generally resulted in darker seeds with increasing redness compared with seeds stored at 6 °C or for 60 d. AAC Y073 and CDC Sol with improved seed coat colour may increase consumer preference, value, and marketability of yellow beans.

Antioxidant activity and zebrafish teratogenicity of hydroalcoholic Moringa oleifera L. leaf extracts

2020 ◽  
Vol 122 (10) ◽  
pp. 3129-3137
Author(s):  
Nurul Fatin Syazleen Mohamad Shariff ◽  
Thangam Singgampalam ◽  
Chean Hui Ng ◽  
Chin Siang Kue
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Moringa Oleifera ◽  
Zebrafish Embryo ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Aqueous Methanol ◽  
Leaf Extracts ◽  
Content Type ◽  
Aqueous Ethanolic Extract

PurposeMoringa oleifera (MO) is a herbal remedy that is rich in essential amino acids and phytochemicals in the leaves, seeds and pods. It is becoming a famous food in various ethnic groups, and the consumption is increasing worldwide. However, there is no safety profile of the hydroalcoholic MO leaf extracts, or documented teratogenicity aspect. This study was sought to compare the antioxidant activity and the teratogenicity of hydroalcoholic MO leaf extracts in the zebrafish embryo.Design/methodology/approachMO leaves were extracted with aqueous alcohols (ethanol or methanol: water, 80:20 v/v) using the Soxhlet method. The antioxidant capacity was determined using DPPH assay. The concentrations of extracts ranging from 3 to 1,000 µg/mL were used to examine the toxicity and teratogenicity on the zebrafish embryo.FindingsBoth hydroalcoholic MO leaf extracts were positive for alkaloids, terpenoids, steroids, tannins, flavonoids, phenols, coumarins, quinones and glycosides, except saponins only absent in aqueous methanol extract. The antioxidant capacity based on lC50, was 15.92 ± 3.62 mg/mL for aqueous methanolic and 25.28 ± 2.89 mg/mL for aqueous ethanolic extract. For acute toxicity, the aqueous methanolic extract has lower LC50 (163.87 ± 12.88 µg/mL) compared to aqueous ethanolic extract (337.48 ± 30.04 µg/mL). The embryo treated with aqueous methanolic extract showed phenotypic defect but not on the aqueous ethanolic extract.Originality/valueThis study suggests that aqueous methanol of MO leaves extract has better antioxidant capacity compared to ethanol, and ethanolic leaf extract is safer (higher LC50 and no teratogenicity) than methanolic extract.

scholarly journals LC-ESI-QTOF/MS Characterization of Phenolic Compounds from Medicinal Plants (Hops and Juniper Berries) and Their Antioxidant Activity

Foods ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 7 ◽  
Author(s):  
Jiafei Tang ◽  
Frank R. Dunshea ◽  
Hafiz A. R. Suleria
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Phenolic Content ◽  
Total Phenolic ◽  
High Concentration ◽  
Antioxidant Power ◽  
Pharmaceutical Industries

Hops (Humulus lupulus L.) and juniper berries (Juniperus communis L.) are two important medicinal plants widely used in the food, beverage, and pharmaceutical industries due to their strong antioxidant capacity, which is attributed to the presence of polyphenols. The present study is conducted to comprehensively characterize polyphenols from hops and juniper berries using liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS) to assess their antioxidant capacity. For polyphenol estimation, total phenolic content, flavonoids and tannins were measured, while for antioxidant capacity, three different antioxidant assays including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay, the 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cation decolorization assay and the ferric reducing-antioxidant power (FRAP) assay were used. Hops presented the higher phenolic content (23.11 ± 0.03 mg/g dw) which corresponded to its strong antioxidant activity as compared to the juniper berries. Using LC-ESI-QTOF/MS, a total of 148 phenolic compounds were tentatively identified in juniper and hops, among which phenolic acids (including hydroxybenzoic acids, hydroxycinnamic acids and hydroxyphenylpropanoic acids) and flavonoids (mainly anthocyanins, flavones, flavonols, and isoflavonoids) were the main polyphenols, which may contribute to their antioxidant capacity. Furthermore, the HPLC quantitative analysis showed that both samples had a high concentration of phenolic acids and flavonoids. In the HPLC quantification, the predominant phenolic acids in hops and juniper berries were chlorogenic acid (16.48 ± 0.03 mg/g dw) and protocatechuic acid (11.46 ± 0.03 mg/g dw), respectively. The obtained results highlight the importance of hops and juniper berries as a rich source of functional ingredients in different food, beverage, and pharmaceutical industries.

scholarly journals Phenolic Profile and Antioxidant Activity of Pulp and Peel from Peach and Nectarine Fruits

2016 ◽  
Vol 44 (1) ◽  
pp. 175-182 ◽  
Author(s):  
Branka T. STOJANOVIC ◽  
Snezana S. MITIC ◽  
Gordana S. STOJANOVIC ◽  
Milan N. MITIC ◽  
Danijela A. KOSTIC ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Prunus Persica ◽  
Biological Activities ◽  
Economic Value ◽  
Antioxidant Properties ◽  
Fruit Peel ◽  
Extraction Solvent ◽  
Phenolic Profiles ◽  
Antioxidant Characteristics

Peach (Prunus persica L.) is a fruit of high nutritional and economic value. Carbohydrates, dietary fibers, minerals and organic acids are among the major constituents of peach fruit, which contribute to the nutritional quality of both fresh fruits and juice. Polyphenolic compounds found in peach may play an important role in physiological functions related to human health. Different polyphenolics may have varied biological activities including antioxidant activity. In this study antioxidant characteristics between peel and pulp of different peach cultivars (‘Radmilovčanka’, ‘June Gold’, ‘Blake’, ‘Hale’, ‘Vesna’, ‘Adria’) and one of nectarine (‘Fantasia’) were investigated. The peel and pulp extracts showed a huge amount of total phenolics (TP), total flavonoids (TF), total hydroxycinnamates (TH) and total flavonols (TFL), ranging from 42.7-211.4, 11.1-128.5 mg GAE/100 g fresh weight (f.w.) (TP), 21.9 -94.9, 5.0-58.9 mg CE/100 g f.w. (TF), 28.4-389.2, 8.5-165.8 mg kg-1 f.w. (TH) and 17.3-54 mg kg-1 f.w. (TFL). High contents of phenolic compounds were significantly correlated with high antioxidant capacities. Peach pulp and peel differ significantly in their phenolic profiles: the pulp contains mainly chlorogenic, neochlorogenic and p-coumaric acids, whereas the peel possesses chlorogenic, neochlorogenic and p-coumaric acids together with several flavonol glycosides in huge amounts. Our results indicate that cultivar and extraction solvent play important roles in phenolic compositions and antioxidant properties of peach and nectarine extracts, which was shown using statistical analysis (ANOVA). There are high correlations between extracted phenolic compounds and peach and nectarine cultivars, and used solvent and part of the fruit (peel and pulp).

scholarly journals Study of preparations of bee pollen extracts, antioxidant and antibacterial activity

2007 ◽  
Vol 31 (6) ◽  
pp. 1818-1825 ◽  
Author(s):  
Solange Teresinha Carpes ◽  
Rosicler Begnini ◽  
Severino Matias de Alencar ◽  
Maria Lúcia Masson
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Activity Index ◽  
Ethanolic Extract ◽  
Ethanol Solution ◽  
Pollen Extract ◽  
Extraction Condition ◽  
Polyphenol Compounds ◽  
Significant Difference

The aim of this study was to determine the antioxidant activity, phenolic content and antibacterial activity of pollen extracts obtained with different concentrations of ethanol. Each extraction condition (ethanol solutions from 40 to 90%) had a different effect in the phenolic compounds content. Although, the pollen extract obtained at 60, 70 and 80% of ethanol showed relatively higher levels of phenolic compounds (>10 mg/g) and did not present statistical significant difference between the extraction conditions. The amount of total phenolics ranged from 3.6 to 8.1 and 6.6 to 10.9 mg GAE/g for Alagoas state and Parana state pollen, respectively. The higher value for antioxidant activity index was 83.30% for the pollen from Alagoas state and 81.15 % for Parana state pollen. The highest degree of antioxidant activity was found in the extraction at 60% of ethanol solution for Parana state pollen, which also showed the highest concentration of polyphenol compounds. Staphylococcus aureus was inhibited by the ethanolic extract of Alagoas state pollen in all the concentrations of solvent, except the ethanolic extract of pollen at 90%. The extract at 60% of ethanol solution (Parana sample) inhibited Bacillus subtilis, Pseudomonas aeruginosa and Klebsiella sp.

scholarly journals Comparison of total phenolic and flavonoids contents in Malaysian propolis extract with two different extraction solvents

2019 ◽  
Vol 6 (2) ◽  
pp. 1-11
Author(s):  
Siti Umairah Mokhtar
Keyword(s):  
Colorimetric Method ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Propolis Extract ◽  
Extraction Solvent ◽  
Percentage Yield

This study is aimed to prepare propolis extracts by using two different solvents which are ethanol and water via maceration method and to evaluate the total phenolic and flavonoid components of the extract. Optimization of the extraction process was done by using different solvents and sample-to-solvent ratio. The ethanolic and water extracts were analysed for the determination of Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) by using Folin-Ciocalteu colorimetric method and Aluminimum chloride method, respectively. Based on sample-to-solvent ratio, it showed that 1:10 gave the highest percentage yield compared to other ratio for both solvents. Ethanolic extract resulted contains high phenolic and flavonoids content compared to water extract. The result showed that sample-to-solvent ratio 1:5 gave highest TPC and TFC in ethanolic extract which are 29.09 and 7.75 mg GAE/g, respectively. In conclusion, this study revealed that ethanol is the best extraction solvent to be used for propolis extraction compared to water. It is also demonstrated that higher volume of solvent does not leads to high values of TPC and TFC. The optimum extraction parameters and conditions are necessary to be obtained in order to extract high TPC and TFC.

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