scholarly journals Caracterización y perfil de susceptibilidad de uropatógenos asociados a la presencia de bacteriuria asintomática en gestantes del departamento del Atlántico, Colombia, 2014-2015. Estudio de corte transversal

2017 ◽  
Vol 68 (1) ◽  
pp. 62 ◽  
Author(s):  
Mirna Luz Campo-Urbina ◽  
Norella Ortega-Ariza ◽  
Alexander Parody-Muñoz ◽  
Lucila Del Carmen Gómez-Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
E Coli ◽  
El Sistema

Objetivo: la bacteriuria asintomática con manejo inadecuado en el embarazo se asocia con pielonefritis que puede llevar a complicaciones maternas y fetales. El objetivo de este estudio fue caracterizar los uropatógenos y su perfil de susceptibilidad, asociados a la presencia de bacteriuria asintomática en una muestra de mujeres gestantes.Materiales y métodos: estudio descriptivo de corte transversal, en gestantes atendidas en el programa de control prenatal en una institución de primer nivel en el departamento del Atlántico, Colombia. Se realizó un muestreo por conveniencia a partir del universo de pacientes atendidas en el programa. Se hizo el diagnóstico de bacteriuria asintomática por la presencia de recuentos ≥ 105 colonias / mL de un solo germen. Para la identificación y determinación del perfil de susceptibilidad de las bacterias aisladas se utilizó el sistema automatizado Phoenix® BD. Se describe la prevalencia de bacteriuria asintomática, y la frecuencia y susceptibilidad por tipo de germen.Resultados: ingresaron 226 gestantes.La frecuencia de bacteriuria asintomática fue del 10,6 %. El uropatógeno más frecuentemente aislado fue la Escherichia coli en un 25 % de los casos, seguida por Enterococcus faecalis en un 20,8 %. La resistencia a la ampicilina fue del 33,3 y 20 % respectivamente; el resto de enterobacterias identificadas presenta una resistencia natural a dicho antibiótico. La resistencia de la E. coli y el E. faecalis a la nitrofurantoína fue del 16,6 y 20 % respectivamente.Conclusiones: se encuentra resistencia importante en el nivel de atención de los gérmenes más frecuentemente aislados en gestantes con bacteriuria asintomática a la ampicilina: en trimetoprim sulfametoxazol fue del 66 %. La nitrofurantoína sigue siendo una buena opción para el tratamiento en la primera mitad del embarazo.

scholarly journals ДОСЛІДЖЕННЯ ПРОТИМІКРОБНИХ ВЛАСТИВОСТЕЙ КОМПОЗИЦІЇ ДЛЯ ЛІКУВАННЯ ЗАПАЛЬНИХ УРАЖЕНЬ ПАРОДОНТУ ВІТЧИЗНЯНОГО ВИРОБНИЦТВА

2021 ◽  
Vol 112 (3) ◽  
pp. 17-24
Author(s):  
О. В. Павленко ◽  
М. В. Кривцова ◽  
О. О. Случевська ◽  
О. М. Ступницька
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Streptococcus Pyogenes ◽  
Culture Collection ◽  
American Type Culture Collection ◽  
E Coli

Ціль дослідження. Запально-деструктивні захворю-вання пародонту в світі становлять не тільки зага-льномедичну, а й соціально-економічну проблему, яка спонукає до пошуку нових методів лікування та діаг-ностики. Сучасні концепції лікування хронічного гене-ралізованого пародонтиту передбачають застосу-вання комплексу терапевтичних, ортопедичних, хіру-ргічних і ортодонтичних методів лікування, і доволі часто їх ефективність є сумнівною.На сьогодні загальновизнаним препаратом вибору в пародонтології є хлоргексидину біглюконат (та його інші сполуки), оскільки він здатний пригнічувати ріст і формування колоній понад 80,00% мікроорганізмів біоплівки ротової порожнини.Також у клінічній практиці застосовують протимік-робні препарати системної дії, особливо у випадках агресивних і важких форм генералізованого пародон-титу, що виникають на фоні загальносоматичної патології, а також при ускладненнях хірургічних втручань. Матеріали та методи дослідження. Для проведення досліджень використовували типові музейні культу-ри ATTC (American Type Culture Collection, USA) Candida albicans ATCC 885–653; Staphylococcus aureus ATCC 25923; Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Streptococcus pyogenes ATCC 19615, та клінічні культури ізольовані із ротової порожнини людей із запальними захворю-ваннями: мікроскопічні гриби роду Candida (C. albicans) Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes, Enterococcus faecalis.Мінімальну бактерицидну та інгібуючу концентрацію досліджуваного гелю визначали у полістиролових пла-стикових 96-лункових планшетах.Результати дослідження та їх обговорення. Ре-зультати дослідження показали, що досліджуваний гель та розчин хлоргексидину біглюконат 0,05% чини-ли активний вплив на музейні та клінічні штами ла-бораторних мікроорганізмів.Висновки. Отже, досліджуваний гель для лікування запальних уражень пародонту вітчизняного виробни-цтва «Джен-Метрогелур» виявився ефективним протимікробним засобом відносно клінічних та лабо-раторних штамів мікроорганізмів. Високочутливими до засобу виявилися клінічні та музейні культури C. albicans. Чутливими до гелю були культури S. aureus та музейні штами S. pyogenes та E. coli. Помірно чу-тливими виявилися культури E. faecalis та клінічні штами S. pyogenes та E. coli.

scholarly journals Detección y caracterización de Escherichia coli O157 de ganado bovino faenado en frigoríficos de la Argentina

2015 ◽  
Author(s):  
◽  
Lourdes Leonor Del Castillo
Keyword(s):  
Escherichia Coli ◽  
E Coli ◽  
El Sistema

El principal objetivo fue estimar la prevalencia y caracterizar las <i>E. coli</i> O157 de ganado vacuno faenado en Argentina. Se recolectaron un total de 1622 muestras de heces y carcasas en 9 frigoríficos de exportación. Todas las muestras se sometieron a separación inmunomagnética y las cepas fueron identificadas por PCR múltiple (rfb<SUB>O157</SUB>, stx<SUB>1</SUB>, stx<SUB>2</SUB>). Se aislaron 54 STEC O157 y 48 <i>E. coli</i> O157 toxina Shiga negativas (EC O157 TSN), de las que se establecieron sus características fenotípicas, genotípicas y las variantes stx. La prevalencia promedio de STEC O157 en materia fecal fue de 4,1% y 2,6% en carcasa; mientras que para EC O157 TSN la incidencia fue de 4,7 y 2,6%; respectivamente. No se observaron diferencias significativas por el género o raza de los animales. Los terneros y vaquillonas presentaron mayores porcentajes de prevalencia de STEC O157 en heces (10,5 y 8,5%, respectivamente). Todas las STEC O157 aisladas albergaban los genes stx<SUB>2</SUB>, eae, ehxA, y fliC<SUB>H7</SUB> y sólo el 16,7% presentó el gen stx<SUB>1</SUB>. El genotipo prevalente fue el stx<SUB>2</SUB>/stx<SUB>2c(vh-a)</SUB>, que también es frecuente en los casos de SUH. Mediante XbaI-PFGE se obtuvieron 29 patrones diferentes y 11 clusters. En cinco oportunidades, las cepas de STEC O157 aisladas de las carcasas fueron idénticas a las cepas de materia fecal. También 7 cepas idénticas se aislaron de carcasas muestreadas en dos visitas consecutivas a dos frigoríficos. Cinco perfiles de fago tipo-PFGE-stx fueron coincidentes con perfiles de cepas recuperadas de SUH. Las técnicas de subtipificación molecular mostraron que las cepas EC O157 TSN presentan un origen filogenético diferente a STEC O157. Se cuantificó la resistencia ácida (RA) por 3 mecanismos y se determinó que el sistema Glutamato-dependiente proporciona mejor protección en desafío ácido. Las EC O157 TSN fueron, en promedio, más resistentes que las cepas STEC O157 por los sistemas descarboxilasa dependientes.

scholarly journals Propiedades antimicrobianas y citotóxicas de un adhesivo de uso ortodóncico adicionado con nanopartículas de plata

2018 ◽  
Vol 12 (22) ◽  
pp. 1
Author(s):  
Liliana Argueta Figueroa ◽  
Ma. Concepción Arenas-Arrocena ◽  
Ana Paulina Díaz–Herrera ◽  
Susana Vanessa García-Benítez ◽  
René García-Contreras
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
E Coli

<p class="p1">El objetivo de este estudio fue evaluar el efecto antibacteriano y la citotoxicidad de un adhesivo de uso ortodóncico con nanopartículas de plata (Ag np’s). Se determinó la actividad antimicrobiana mediante una prueba de difusión en disco para <em>Staphylococcus aureus</em>, <em>Escherichia coli</em>, y <em>Enterococcus faecalis </em>a diferentes concentraciones de nanopartículas de plata en un adhesivo ortodóncico. También se evaluó la citotoxicidad de dicho adhesivo al ser incorporadas<span class="Apple-converted-space"> </span>Ag np’s, mediante el método de mtt. Los resultados mostraron que las bacterias en estudio, <em>S. aureus</em>, <em>E. coli </em>y <em>E. faecalis </em>fueron susceptibles tanto a las Ag np’s como al adhesivo mip ortodóncico adicionado con Ag np’s a concentraciones de 10, 5 y 3 v/v%.<span class="Apple-converted-space"> </span></p><p class="p2">El efecto antibacteriano de las nanopartículas de plata es dosis dependiente. La citotoxicidad del adhesivo mip ortodóncico en sí mismo exhibe una citotoxidad ligera a moderada, así como al incorporarle las Ag np’s a las concentraciones antes mencionadas. Las nanopartículas incorporadas en el adhesivo de uso ortodóncico muestran efecto antibacterial así como mantienen una citotoxicidad ligera.<span class="Apple-converted-space"> </span></p>

Phytochemical Extracts of Leucas Aspera and Dahlia Pinnata Exhibit Antimicrobial Properties on Escherichia Coli and Enterococcus Faecalis.

2020 ◽  
Vol 09 ◽  
Author(s):  
Yamuna Saravanan ◽  
Bala Subramaniam Devaraj ◽  
Nithesh Kumar Velusamy ◽  
Pooja Shree Soundirarajan ◽  
Kumaravel Kandaswamy
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Bacterial Infections ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Efficacy Evaluation ◽  
E Coli ◽  
Methanol Extracts ◽  
Leucas Aspera

Background:: Phytochemicals such as tannins, alkaloids, flavonoids, and peptides been found to have antimicrobial activity against a variety of bacterial strains. Objective:: However, optimal extraction procedures for these phytochemicals and their efficacy evaluation against certain pathogenic bacterial strains remain unclear. Method:: Therefore, in this study phytochemicals from Leucas aspera (L. aspera) and Dahlia pinnata (D. pinnata) were extracted by hot and cold extraction methods using water and methanol as solvents. In addition, antimicrobial activity of L. aspera and D. pinnata extracts against bacterial strains such as the gram-negative Escherichia coli (E. coli) and the gram-positive Enterococcus faecalis (E. faecalis) were performed by Minimal Inhibitory Concentration (MIC) and CFU quantification assays. Results:: The majority of the phytochemicals such as protein, carbohydrate, tannins, flavonoids, phenols, and saponins were present in our extracts but steroids were absent in the extract. Protein, tannins, flavonoids, phenols, and saponins were present in both L. aspera and D. pinnata. The yield of proteins was high (1.990 ± 0.091 mg/ml) in methanol extracts of L. aspera and low (0.199 mg/ml) in aqueous extracts. However, the yield of tannins was high (1.713 ± 0.079 mg/ml) in methanol extracts of D. pinnata and low (0.528 ± 0.136 mg/ml) aqueous extracts. The MIC of D. pinnata extracts were found to be 200 mg/ml for both E. coli and E. faecalis. However the L. aspera extracts had a MIC of 100 mg/ml and 200 mg/ml on E. coli and E. faecalis, respectively. Conclusion:: This article demonstrated the potential use of phytochemicals as novel antimicrobial compounds against bacterial infections.

scholarly journals Co-Infection of Escherichia coli, Enterococcus faecalis and Chlamydia psittaci Contributes to Salpingitis of Laying Layers and Breeder Ducks

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 755
Author(s):  
Huanxin Fang ◽  
Hongkun Quan ◽  
Yuhang Zhang ◽  
Qiang Li ◽  
Yihui Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Artificial Insemination ◽  
Egg Production ◽  
Laying Hens ◽  
Secondary Infection ◽  
Chlamydia Psittaci ◽  
Control Group ◽  
Biochemical Tests ◽  
E Coli

Salpingitis is manifested as hemorrhagic follicular inflammation exudations and peritonitis, leading to reduced egg production and high culling of breeder flocks. From 2018 to 2021, increasing salpingitis during egg peak is threatening the poultry industry post-artificial insemination, both in breeder layers and breeder ducks across China. In our study, Escherichia coli (E. coli), Enterococcus faecalis (E. faecalis) and Chlamydia psittaci (C. psittaci) were isolated and identified from the diseased oviducts using biochemical tests and PCR. To identify and isolate pathogenicity, we inoculated the isolates into laying hens via an intravaginal route. Later, laying hens developed typical salpingitis after receiving the combination of the aforementioned three isolates (1 × 105 IFU/mL of C. psittaci and 1 × 106 CFU/mL of E. faecalis and E. coli, respectively), while less oviduct inflammation was observed in the layers inoculated with the above isolate alone. Furthermore, 56 breeder ducks were divided into seven groups, eight ducks per group. The birds received the combination of three isolates, synergic infection of E. coli and E. faecalis, and C. psittaci alone via vaginal tract, while the remaining ducks were inoculated with physiological saline as the control group. Egg production was monitored daily and lesions of oviducts and follicles were determined post-infection on day 6. Interestingly, typical salpingitis, degenerated follicles and yolk peritonitis were obviously found in the synergic infection of three isolates and the birds inoculated with C. psittaci alone developed hemorrhagic follicles and white exudates in oviducts, while birds with E. faecalis or E. coli alone did not develop typical salpingitis. Finally, higher E. coli loads were determined in the oviducts as compared to E. faecalis and C. psittaci infection. Taken together, the combination of E. coli and E. faecalis, and C. psittaci could induce typical salpingitis and yolk peritonitis both in laying hens and breeder ducks. Secondary infection of E. coli and E. faecalis via artificial insemination is urgently needed for investigation against salpingitis.

scholarly journals In Vitro Reduction of Interleukin-8 Response to Enterococcus faecalis by Escherichia coli Strains Isolated from the Same Polymicrobial Urines

2021 ◽  
Vol 9 (7) ◽  
pp. 1501
Author(s):  
Gabriella Piatti ◽  
Laura De Ferrari ◽  
Anna Maria Schito ◽  
Anna Maria Riccio ◽  
Susanna Penco ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Enterococcus Faecalis ◽  
Interleukin 8 ◽  
Fluoroquinolone Resistance ◽  
Epithelial Cell Line ◽  
E Coli ◽  
Tract Infections ◽  
K 12

Urinary tract infections are often polymicrobial and are mainly due to uropathogenic Escherichia coli (UPEC). We previously demonstrated a link among clinical fluoroquinolone susceptible E. coli reducing in vitro urothelial interleukin-8 (CXCL8) induced by E. coli K-12, polymicrobial cystitis, and pyuria absence. Here, we evaluated whether fifteen clinical fluoroquinolone susceptible UPEC were able to reduce CXCL8 induced by Enterococcus faecalis that had been isolated from the same mixed urines, other than CXCL8 induced by E. coli K-12. We also evaluated the connection between fluoroquinolone susceptibility and pathogenicity by evaluating the immune modulation of isogenic gyrA, a mutant UPEC resistant to ciprofloxacin. Using the 5637 bladder epithelial cell line, we observed that lower CXCL8 induced the most UPEC isolates than K-12 and the corresponding E. faecalis. During coinfections of UPEC/K-12 and UPEC/E. faecalis, we observed lower CXCL8 than during infections caused by K-12 and E. faecalis alone. UPEC strains showed host–pathogen and pathogen–pathogen interaction, which in part explained their persistence in the human urinary tract and coinfections, respectively. Mutant UPEC showed lower modulating activity with respect to the wildtypes, confirming the connection between acquired fluoroquinolone resistance and the decrease of innate microbial properties.

scholarly journals Effect of sodium hypochlorite solution and gel with/without passive ultrasonic irrigation on Enterococcus faecalis, Escherichia coli and their endotoxins

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 642
Author(s):  
Amjad Abu Hasna ◽  
Laiana Pereira Da Silva ◽  
Fernanda Carvalho Pelegrini ◽  
Cláudia Luísa Ribeiro Ferreira ◽  
Luciane Dias de Oliveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Ethylenediaminetetraacetic Acid ◽  
Lipoteichoic Acid ◽  
Sodium Hypochlorite Solution ◽  
Passive Ultrasonic Irrigation ◽  
E Coli ◽  
Significant Difference ◽  
Ultrasonic Irrigation

Background: Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics. The purpose of this study was to evaluate the effect of NaOCl solution (2.5%) and gel (3%) with/without passive ultrasonic irrigation (PUI) on Enterococcus faecalis, Escherichia coli, and their endotoxins, lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Methods: 40 human lower premolars were contaminated with E. coli (ATCC 25922) for 28 days and E. faecalis (ATCC 29212) for 21 days. Specimens were randomly divided into four groups: (1) 2.5% NaOCl irrigating the canals without PUI activation; (2) 2.5% NaOCl with PUI; (3) 3% NaOCl gel irrigating the canals without PUI; and (4) 3% NaOCl gel with PUI. 40 mL of irrigant was used for each group. PUI activation was carried out using E1-Irrisonic stainless-steel tip at 10% frequency. After treatment, all specimens were filled with 3mL of 17% ethylenediaminetetraacetic acid (EDTA) for 3min and then washed with nonpyrogenic saline solution. Three samples were collected from the canals: S1, at baseline to confirm biofilm formation; S2 after treatment; and S3 after EDTA. Samples were assessed for E. coli and E. faecalis colony forming units, and LPS and LTA were assessed using chromogenic kinetic LAL assay and ELISA, respectively. Data were analyzed by Kruskal-Wallis, Friedmann and Dunn tests with α≤0.05. Results: All groups were effective in reducing the microbial load of E. coli and E. faecalis after treatment without a significant difference among the groups. NaOCl and NaOCl gel groups had no significant difference in reducing LPS and LTA. Statistically increased reduction was seen for NaOCL + PUI and NaOCl gel + PUI compared for groups without PUI. Conclusions: NaOCl gel has the same antimicrobial action of NaOCl solution and can partially detoxify endotoxins. PUI improves NaOCl (gel or solution) action over E. faecalis and E. coli and their endotoxins.

Removal of Escherichia coli and Enterococcus faecalis after Hand Washing with Antimicrobial and Nonantimicrobial Soap and Persistence of These Bacteria in Rinsates

2017 ◽  
Vol 80 (10) ◽  
pp. 1670-1675 ◽  
Author(s):  
J. Pérez-Garza ◽  
S. García ◽  
N. Heredia
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Membrane Filtration ◽  
Chlorhexidine Gluconate ◽  
Hand Washing ◽  
Distilled Water ◽  
Antimicrobial Compounds ◽  
Filtration Method ◽  
E Coli ◽  
Reducing Bacteria

ABSTRACT Foodhandlers are important sources of contamination in the agricultural environment. This study was conducted (i) to evaluate the activity of antimicrobial soaps against Escherichia coli and Enterococcus faecalis using a hand washing model with soiled hands and (ii) to determine the survival and persistence of these bacteria in rinsates. Sterilized agricultural soil from tomato and pepper farms was inoculated with E. coli or E. faecalis at 103 or 106 CFU/g. Decontaminated hands were placed in contact with contaminated soil for 2 min and were then washed with soaps with or without antimicrobial compounds (citric extracts, chloroxylenol, triclosan, or chlorhexidine gluconate). As the control, hands were washed with sterile distilled water. The levels of bacteria remaining on the hands and recovered from the rinsates were determined using a membrane filtration method and selective media. Antimicrobial soaps removed levels of E. coli similar to those removed by distilled water and nonantimicrobial soap on hands contaminated with E. coli at 103 CFU/g. However, when hands were contaminated with E. coli at 106 CFU/g, more E. coli was removed with the chlorhexidine gluconate soap. When hands were contaminated with E. faecalis at 103 CFU/g, bacteria were removed more effectively with soaps containing chloroxylenol or chlorhexidine gluconate. When hands were contaminated with E. faecalis at 106 CFU/g, all of the antimicrobial soaps were more effective for removing the bacteria than were distilled water and nonantimicrobial soap. E. coli grew in all of the hand washing rinsates except that containing triclosan, whereas E. faecalis from the 106 CFU/g treatments grew in rinsates containing chlorhexidine gluconate and in the distilled water rinsates. Washing with antimicrobial soap was more effective for reducing bacteria on soiled hands than was washing with water or nonantimicrobial soap. However, persistence or growth of bacteria in these rinsates poses health risks.

scholarly journals Different Biliary Microbial Flora Influence Type of Complications after Pancreaticoduodenectomy: A Single Center Retrospective Analysis

2021 ◽  
Vol 10 (10) ◽  
pp. 2180
Author(s):  
Alessandro Coppola ◽  
Vincenzo La Vaccara ◽  
Tommaso Farolfi ◽  
Michele Fiore ◽  
Chiara Cascone ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Postoperative Complications ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Postoperative Morbidity ◽  
Bacterial Species ◽  
Infectious Complications ◽  
E Coli ◽  
Culture Positive

Background: Bacterobilia is associated with postoperative morbidity after pancreaticoduodenectomy (PD), mostly due to infectious complications. The aim of this study was to investigate the prevalence of bacteria species isolated from intraoperative biliary cultures, and related complications after PD. Methods: An ANOVA test was used to assess the prevalence of isolated bacterial species and postoperative complications. The odds ratio was computed to evaluate the association between biliary cultures and each complication, Endoscopic Retrograde CholangioPancreatography (ERCP) and each complication, ERCP and biliary cultures, Delayed Gastric Emptying (DGE) and Postoperative Pancreatic Fistula (POPF). Results: Positive biliary cultures were found in 162/244 (66%) PDs. Different prevalences of polymicrobial biliary culture were detected in patients with postoperative complications. In SSIs, a significant prevalence of biliary culture positive for E. coli, Klebsiella pneumoniæ and Enterococcus fæcalis (p < 0.001) was detected. Prevalences of polymicrobial biliary cultures with Escherichia coli, Klebsiella pneumoniæ, Enterococcus fæcalis and Enterococcus fæcium were significantly associated with POPF (p < 0.001). Biliary culture positive for Escherichia coli, Enterococcus fæcalis and Enterococcus fæcium showed a higher prevalence of intra-abdominal collection and DGE (p < 0.001). Notably, Escherichia coli was significantly associated with DGE as a unique complication (OR = 2.94 (1.30–6.70); p < 0.01). Conclusions: Specific prevalences of polymicrobial bacterobilia are associated with major complications, while monomicrobial Escherichia coli bacterobilia is associated with DGE as a unique complication after PD.

scholarly journals Identificación de microbiota bucal en caninos en estado de abandono

Nova ◽  
2019 ◽  
Vol 17 (32) ◽  
pp. 39-64
Author(s):  
Lucia Constanza Corrales ◽  
Diana Marcela Antolinez Romero ◽  
Johanna Azucena Bohórquez Macías ◽  
Aura Marcela Corredor Vargas
Keyword(s):  
Escherichia Coli ◽  
Enterococcus Faecalis ◽  
Proteus Mirabilis ◽  
Enterobacter Cloacae ◽  
Citrobacter Freundii ◽  
Enterobacter Sakazakii ◽  
Eikenella Corrodens ◽  
Enterococcus Durans ◽  
El Sistema

Objetivo. Describir la microbiota que se encuentra en la cavidad bucal de caninos en condición de abandono de la Fundación Razas Únicas en el municipio de Chía – Cundinamarca. Métodos. Para el estudio se tomaron 29 muestras orales con escobillón a 23 caninos de la Fundación Razas Únicas del municipio de Chía – Cundinamarca. 23 muestras se recolectaron para identificación de bacterias aerobias y anaerobias facultativas,  las cuales se transportaron en medio líquido tripticasa soya y 6 muestras para recuperación de bacterias anaerobias estrictas transportadas en medio VMGA-III. El aislamiento de los microorganismos se realizó en medios selectivos y la identificación con el sistema BD BBL™Crystal™. Resultados. De las 29 muestras analizadas se aislaron 59 bacterias, entre ellas 15 géneros y 15 especies diferentes como; Escherichia coli, Proteus mirabilis, Citrobacter freundii, Enterobacter sakazakii, Enterobacter cloacae, Enterococcus durans, Enterococcus faecalis, Eikenella corrodens, Porphyromonas endodontalis, Fusobacterium spp y Capnocytophaga spp. De acuerdo con la revisión de la literatura, las bacterias anaerobias encontradas están principalmente relacionadas con enfermedad periodontal y las enterobacterias con contaminación oro-fecal.

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