Deciphering the dynamic gene expression patterns of pollen abortion in a male sterile line of Avena sativa through transcriptome analysis at different developmental stages

Abstract Background Male sterility (MS) has important applications in hybrid seed production, and the abortion of anthers has been observed in many plant species. While most studies have focused on the genetic factors affecting male sterility, the dynamic gene expression patterns of pollen abortion in male sterile lines have not been fully elucidated. In addition, there is still no hybrid oat that is commercially planted due to the lack of a suitable system of male sterility for hybrid breeding. Results In this study, we cultivated a male sterile oat line and a near-isogenic line by crossbreeding to elucidate the expression patterns of genes that may be involved in sterility. The first reported CA male sterile (CAMS) oat line was used for cross-testing and hybridization experiments and was confirmed to exhibit a type of nuclear sterility controlled by recessive genes. Oat stamens of two lines were sampled at four different developmental stages separately. Paired-end RNA sequencing was performed for each sample and generated 252.84 Gb sequences. There were 295,462 unigenes annotated in public databases in all samples, and we compared the histological characteristics and transcriptomes of oat stamens from the two oat lines at different developmental stages. Our results demonstrate that the sterility of the male sterile oat line occurs in the early stage of stamen development and is primarily attributable to abnormal meiosis and the excessive accumulation of superoxide. Conclusions To the best of our knowledge, this study is the first to decipher the dynamic expression profiles of pollen abortion CAMS and CA male fertile (CAMF) oat lines, which may represent a valuable resource for further studies attempting to understand pollen abortion and anther development in oats.

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Cytological observation of anther structure and genetic investigation of a thermo-sensitive genic male sterile line 373S in Brassica napus L

BMC Plant Biology ◽

10.1186/s12870-019-2220-1 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Yanyan Sun ◽

Dongsuo Zhang ◽

Zhenzhen Wang ◽

Yuan Guo ◽

Xiaomin Sun ◽

...

Keyword(s):

Brassica Napus ◽

Male Sterility ◽

Spontaneous Mutation ◽

Hybrid Breeding ◽

Pcr Analysis ◽

Cytological Observation ◽

Male Sterile ◽

Pollen Abortion ◽

Genetic Investigation ◽

Temperature Changes

Abstract Background Photoperiod and/or thermo-sensitive male sterility is an effective pollination control system in crop two-line hybrid breeding. We previously discovered the spontaneous mutation of a partially male sterile plant and developed a thermo-sensitive genic male sterile (TGMS) line 373S in Brassica napus L. The present study characterized this TGMS line through cytological observation, photoperiod/ temperature treatments, and genetic investigation. Results Microscopic observation revealed that the condensed cytoplasm and irregular exine of microspores and the abnormal degradation of tapetum are related to pollen abortion. Different temperature and photoperiod treatments in field and growth cabinet conditions indicated that the fertility alteration of 373S was mainly caused by temperature changes. The effects of photoperiod and interaction between temperature and photoperiod were insignificant. The critical temperature leading to fertility alteration ranged from 10 °C (15 °C/5 °C) to 12 °C (17 °C/7 °C), and the temperature-responding stage was coincident with anther development from pollen mother cell formation to meiosis stages. Genetic analysis indicated that the TGMS trait in 373S was controlled by one pair of genes, with male sterility as the recessive. Multiplex PCR analysis revealed that the cytoplasm of 373S is pol type. Conclusions Our study suggested that the 373S line in B. napus has a novel thermo-sensitive gene Bnmst1 in Pol CMS cytoplasm background, and its fertility alteration is mainly caused by temperature changes. Our results will broaden the TGMS resources and lay the foundation for two-line hybrid breeding in B. napus.

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Microarray analysis of gene expression patterns in the leaf during potato tuberization in the potato somatic hybrid Solanum tuberosum and Solanum etuberosum

Genome ◽

10.1139/gen-2014-0191 ◽

2015 ◽

Vol 58 (6) ◽

pp. 305-313 ◽

Cited By ~ 6

Author(s):

Jagesh Kumar Tiwari ◽

Sapna Devi ◽

S. Sundaresha ◽

Poonam Chandel ◽

Nilofer Ali ◽

...

Keyword(s):

Gene Expression ◽

Candidate Genes ◽

Microarray Analysis ◽

Somatic Hybrid ◽

Developmental Stages ◽

Expression Patterns ◽

Pcr Analysis ◽

Gene Expression Patterns ◽

Potato Tuberization ◽

Solanum Etuberosum

Genes involved in photoassimilate partitioning and changes in hormonal balance are important for potato tuberization. In the present study, we investigated gene expression patterns in the tuber-bearing potato somatic hybrid (E1-3) and control non-tuberous wild species Solanum etuberosum (Etb) by microarray. Plants were grown under controlled conditions and leaves were collected at eight tuber developmental stages for microarray analysis. A t-test analysis identified a total of 468 genes (94 up-regulated and 374 down-regulated) that were statistically significant (p ≤ 0.05) and differentially expressed in E1-3 and Etb. Gene Ontology (GO) characterization of the 468 genes revealed that 145 were annotated and 323 were of unknown function. Further, these 145 genes were grouped based on GO biological processes followed by molecular function and (or) PGSC description into 15 gene sets, namely (1) transport, (2) metabolic process, (3) biological process, (4) photosynthesis, (5) oxidation-reduction, (6) transcription, (7) translation, (8) binding, (9) protein phosphorylation, (10) protein folding, (11) ubiquitin-dependent protein catabolic process, (12) RNA processing, (13) negative regulation of protein, (14) methylation, and (15) mitosis. RT-PCR analysis of 10 selected highly significant genes (p ≤ 0.01) confirmed the microarray results. Overall, we show that candidate genes induced in leaves of E1-3 were implicated in tuberization processes such as transport, carbohydrate metabolism, phytohormones, and transcription/translation/binding functions. Hence, our results provide an insight into the candidate genes induced in leaf tissues during tuberization in E1-3.

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Photosynthetic Gene Expression in Amaranth, an NAD-ME Type C4 Dicot

Functional Plant Biology ◽

10.1071/pp97001 ◽

1997 ◽

Vol 24 (4) ◽

pp. 423 ◽

Cited By ~ 11

Author(s):

James O. Berry ◽

Dennis J. McCormac ◽

John J. Long ◽

Joseph Boinski ◽

Amy C. Corey

Keyword(s):

Gene Expression ◽

Developmental Stages ◽

Expression Patterns ◽

Metabolic Enzyme ◽

Gene Expression Patterns ◽

Control Mechanisms ◽

Multiple Control ◽

Amaranthus Hypochondriacus ◽

Specific Accumulation ◽

Post Transcriptional Regulation

Post-transcriptional regulation determines initial C4 gene expression patterns in developing leaves of Amaranthus hypochondriacus, an NAD-ME type C4 dicot. RuBPCase, PEPCase, and PPdK mRNAs are abundant in meristems and in leaf primordia, but are utilised only during specific developmental stages. While each enzyme shows independent patterns of initial mRNA and polypeptide accumulation, cell-specific localisation of the polypeptides occurs prior to cell-specific localisation of the mRNAs. In mature three-coloured leaves of A. tricolor, loss of photosynthetic activity correlates with reductions in the transcription rates of some plastid-encoded genes, reduction and loss of coordination in the translation of RuBPCase polypeptides, and loss of cell-specific accumulation of RuBPCase mRNAs (but not the polypeptides). The mitochondrial NAD-dependent malic enzyme (NAD-ME) provides an example of a basic metabolic enzyme that has acquired specialised gene expression patterns allowing it to function in the C4 pathway. NAD-ME occurs preferentially in photosynthetic tissues, and is specific to bundle sheath cells throughout development. NAD-ME synthesis is regulated by light and development at transcriptional and post-transcriptional levels. The findings summarised here indicate that C4 genes are independently regulated by multiple control mechanisms in response to developmental, environmental, and metabolic signals.

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Evolution of gene expression across species and specialized zooids in Siphonophora

10.1101/2021.07.30.454354 ◽

2021 ◽

Author(s):

Catriona Munro ◽

Felipe Zapata ◽

Mark Howison ◽

Stefan Siebert ◽

Casey W Dunn

Keyword(s):

Gene Expression ◽

Large Scale ◽

Developmental Stages ◽

Expression Patterns ◽

Gene Expression Patterns ◽

Gene Trees ◽

Species Trees ◽

Examine Gene Expression ◽

Specialized Tissue ◽

Species Specific

Background: Siphonophores are complex colonial animals, consisting of asexually-produced bodies (called zooids) that are functionally specialized for specific tasks, including feeding, swimming, and sexual reproduction. Though this extreme functional specialization has captivated biologists for generations, its genomic underpinnings remain unknown. We use RNA-seq to investigate gene expression patterns in five zooids and one specialized tissue (pneumatophore) across seven siphonophore species. Analyses of gene expression across species present several challenges, including identification of comparable expression changes on gene trees with complex histories of speciation, duplication, and loss. Here, we conduct three analyses of expression. First, we examine gene expression within species. Then, we conduct classical analyses examining expression patterns between species. Lastly, we introduce Speciation Branch Filtering, which allows us to examine the evolution of expression in a phylogenetic framework. Results: Within and across species, we identified hundreds of zooid-specific and species-specific genes, as well as a number of putative transcription factors showing differential expression in particular zooids and developmental stages. We found that gene expression patterns tended to be largely consistent in zooids with the same function across species, but also some large lineage-specific shifts in gene expression. Conclusions: Our findings show that patterns of gene expression have the potential to define zooids in colonial organisms. We also show that traditional analyses of the evolution of gene expression focus on the tips of gene phylogenies, identifying large-scale expression patterns that are zooid or species variable. The new explicit phylogenetic approach we propose here focuses on branches (not tips) offering a deeper evolutionary perspective into specific changes in gene expression within zooids along all branches of the gene (and species) trees.

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Chasing the Apomictic Factors in the Ranunculus auricomus Complex: Exploring Gene Expression Patterns in Microdissected Sexual and Apomictic Ovules

Genes ◽

10.3390/genes11070728 ◽

2020 ◽

Vol 11 (7) ◽

pp. 728 ◽

Cited By ~ 2

Author(s):

Marco Pellino ◽

Diego Hojsgaard ◽

Elvira Hörandl ◽

Timothy F. Sharbel

Keyword(s):

Gene Expression ◽

Asexual Reproduction ◽

Developmental Stages ◽

Expression Patterns ◽

Dominant Factor ◽

Gene Expression Patterns ◽

Future Studies ◽

Dosage Effects ◽

Differential Gene ◽

Parent Of Origin

Apomixis, the asexual reproduction via seeds, is associated to polyploidy and hybridization. To identify possible signatures of apomixis, and possible candidate genes underlying the shift from sex to apomixis, microarray-based gene expression patterns of live microdissected ovules at four different developmental stages were compared between apomictic and sexual individuals of the Ranunculus auricomus complex. Following predictions from previous work on mechanisms underlying apomixis penetrance and expressivity in the genus, gene expression patterns were classified into three categories based on their relative expression in apomicts compared to their sexual parental ancestors. We found evidence of misregulation and differential gene expression between apomicts and sexuals, with the highest number of differences detected during meiosis progression and emergence of aposporous initial (AI) cells, a key developmental stage in the ovule of apomicts where a decision between divergent reproductive pathways takes place. While most of the differentially expressed genes (DEGs) could not be annotated, gene expression was classified into transgressive, parent of origin and ploidy effects. Genes related to gametogenesis and meiosis demonstrated patterns reflective of transgressive and genome dosage effects, which support the hypothesis of a dominant factor controlling apomixis in Ranunculus and modulated by secondary modifiers. Three genes with probable functions in sporogenesis and gametogenesis development are identified and characterized for future studies.

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Transcriptome Reveals Allele Contribution to Heterosis in Maize

Frontiers in Plant Science ◽

10.3389/fpls.2021.739072 ◽

2021 ◽

Vol 12 ◽

Author(s):

Jianzhong Wu ◽

Dequan Sun ◽

Qian Zhao ◽

Hongjun Yong ◽

Degui Zhang ◽

...

Keyword(s):

Gene Expression ◽

Developmental Stages ◽

Expression Patterns ◽

Quantitative Variation ◽

Allelic Expression ◽

Gene Expression Patterns ◽

Gene Expression Variation ◽

Specific Expression ◽

Maize Hybrids ◽

Expression Variation

Heterosis, which has greatly increased maize yields, is associated with gene expression patterns during key developmental stages that enhance hybrid phenotypes relative to parental phenotypes. Before heterosis can be more effectively used for crop improvement, hybrid maize developmental gene expression patterns must be better understood. Here, six maize hybrids, including the popular hybrid Zhengdan958 (ZC) from China, were studied. Maize hybrids created in-house were generated using an incomplete diallel cross (NCII)-based strategy from four elite inbred parental lines. Differential gene expression (DEG) profiles corresponding to three developmental stages revealed that hybrid partial expression patterns exhibited complementarity of expression of certain parental genes, with parental allelic expression patterns varying both qualitatively and quantitatively in hybrids. Single-parent expression (SPE) and parent-specific expression (PSE) types of qualitative variation were most prevalent, 43.73 and 41.07% of variation, respectively. Meanwhile, negative super-dominance (NSD) and positive super-dominance (PSD) types of quantitative variation were most prevalent, 31.06 and 24.30% of variation, respectively. During the early reproductive growth stage, the gene expression pattern differed markedly from other developmental stage patterns, with allelic expression patterns during seed development skewed toward low-value parental alleles in hybrid seeds exhibiting significant quantitative variation-associated superiority. Comparisons of qualitative gene expression variation rates between ZC and other hybrids revealed proportions of SPE-DEGs (41.36%) in ZC seed DEGs that significantly exceeded the average proportion of SPE-DEGs found in seeds of other hybrids (28.36%). Importantly, quantitative gene expression variation rate comparisons between ZC and hybrids, except for transgressive expression, revealed that the ZC rate exceeded the average rate for other hybrids, highlighting the importance of partial gene expression in heterosis. Moreover, enriched ZC DEGs exhibiting distinct tissue-specific expression patterns belonged to four biological pathways, including photosynthesis, plant hormone signal transduction, biology metabolism and biosynthesis. These results provide valuable technical insights for creating hybrids exhibiting strong heterosis.

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Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of ZmMs20 Gene Regulating Male Fertility in Maize

International Journal of Molecular Sciences ◽

10.3390/ijms20061411 ◽

2019 ◽

Vol 20 (6) ◽

pp. 1411 ◽

Cited By ~ 5

Author(s):

Yanbo Wang ◽

Dongcheng Liu ◽

Youhui Tian ◽

Suowei Wu ◽

Xueli An ◽

...

Keyword(s):

Male Sterility ◽

Pollen Development ◽

Male Fertility ◽

Molecular Mechanisms ◽

Expression Patterns ◽

Hybrid Breeding ◽

Functional Markers ◽

Hybrid Seed Production ◽

Functional Relationships ◽

Zea May

Genic male sterility (GMS) mutant is a useful germplasm resource for both theory research and production practice. The identification and characterization of GMS genes, and assessment of male-sterility stability of GMS mutant under different genetic backgrounds in Zea may (maize) have (1) deepened our understanding of the molecular mechanisms controlling anther and pollen development, and (2) enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for hybrid breeding. Here, we reported a complete GMS mutant (ms20), which displays abnormal anther cuticle and pollen development. Its fertility restorer gene ZmMs20 was found to be a new allele of IPE1 encoding a glucose methanol choline (GMC) oxidoreductase involved in lipid metabolism in anther. Phylogenetic and microsynteny analyses showed that ZmMs20 was conserved among gramineous species, which provide clues for creating GMS materials in other crops. Additionally, among the 17 maize cloned GMS genes, ZmMs20 was found to be similar to the expression patterns of Ms7, Ms26, Ms6021, APV1, and IG1 genes, which will give some clues for deciphering their functional relationships in regulating male fertility. Finally, two functional markers of ZmMs20/ms20 were developed and tested for creating maize ms20 male-sterility lines in 353 genetic backgrounds, and then an artificial maintainer line of ms20 GMS mutation was created by using ZmMs20 gene, ms20 mutant, and BMS system. This work will promote our understanding of functional mechanisms of male fertility and facilitate molecular breeding of ms20 male-sterility lines for hybrid seed production in maize.

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The Ontology of the Amphioxus Anatomy and Life Cycle (AMPHX)

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.668025 ◽

2021 ◽

Vol 9 ◽

Author(s):

Stephanie Bertrand ◽

João E. Carvalho ◽

Delphine Dauga ◽

Nicolas Matentzoglu ◽

Vladimir Daric ◽

...

Keyword(s):

Gene Expression ◽

Scientific Community ◽

Developmental Stages ◽

Expression Patterns ◽

Evolutionary Developmental Biology ◽

Model Organisms ◽

Gene Expression Patterns ◽

Marine Animal ◽

Computable Representation ◽

Different Parts

An ontology is a computable representation of the different parts of an organism and its different developmental stages as well as the relationships between them. The ontology of model organisms is therefore a fundamental tool for a multitude of bioinformatics and comparative analyses. The cephalochordate amphioxus is a marine animal representing the earliest diverging evolutionary lineage of chordates. Furthermore, its morphology, its anatomy and its genome can be considered as prototypes of the chordate phylum. For these reasons, amphioxus is a very important animal model for evolutionary developmental biology studies aimed at understanding the origin and diversification of vertebrates. Here, we have constructed an amphioxus ontology (AMPHX) which combines anatomical and developmental terms and includes the relationships between these terms. AMPHX will be used to annotate amphioxus gene expression patterns as well as phenotypes. We encourage the scientific community to adopt this amphioxus ontology and send recommendations for future updates and improvements.

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Morphological and Cytological Characterization of Petaloid-type Cytoplasmic Male Sterility in Camellia oleifera

HortScience ◽

10.21273/hortsci14005-19 ◽

2019 ◽

Vol 54 (7) ◽

pp. 1149-1155

Author(s):

Huan Xiong ◽

Ping Chen ◽

Zhoujun Zhu ◽

Ya Chen ◽

Feng Zou ◽

...

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Abnormal Development ◽

Hybrid Breeding ◽

Vascular Bundles ◽

Camellia Oleifera ◽

Sterile Male ◽

Male Sterile ◽

Pollen Abortion ◽

Cytological Characteristics

Camellia oleifera is an important woody tree species in China that produces edible oil. Although sterile male C. oleifera plants play an important role in hybrid breeding, the possible cytological characteristics of pollen abortion remain unknown. To characterize the pollen abortion process, a genic petaloid-type sterile male C. oleifera ‘X1’ plant was investigated using a cytological method. The results showed that in male-fertile plants, the anthers were full and butterfly shaped, the pollen viability was as high as 97.5%, and the development of the tapetum and anther vascular bundles was normal. However, in male-sterile C. oleifera ‘X1’, petaloidy in the anther was observed, and the pollen vitality was as low as 4.5%. Pollen abortion in sterile C. oleifera ‘X1’ anthers occurred from the microspore stage to the mature pollen period. Further cytological analyses revealed an abnormally enlarged tapetum and retarded tapetum degeneration, suggesting that insufficient nutrients were provided for microspore development. Moreover, the anther vascular bundles displayed hyperplasia, and the pollen sac area became increasingly smaller, causing most anthers to be sterile and to have few pollen grains. Taken together, the results indicate that petaloid-type male sterility in C. oleifera may be attributed to abnormal development of the tapetum and anther vascular bundles. The findings clarify the pollen abortion period and the cytological characteristics of petaloid-type cytoplasmic male sterility in C. oleifera, and lay a solid foundation for the male sterile line in C. oleifera hybrid breeding.

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Mitochondrial Proteomics and Transcriptional Analysis of Cytoplasmic Male Sterility in Sugar Beet using iTRAQ and qRT–PCR

10.21203/rs.3.rs-1120889/v1 ◽

2022 ◽

Author(s):

Tianjiao Liu ◽

Dayou Cheng ◽

Xue Han ◽

Jie Cui ◽

Cuihong Dai ◽

...

Keyword(s):

Sugar Beet ◽

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Raw Material ◽

Differentially Expressed ◽

Transcriptional Analysis ◽

Hybrid Breeding ◽

Male Sterile ◽

Pollen Abortion ◽

Absolute Quantitation

Abstract Sugar beet (Beta vulgaris L.) is an important raw material for the sugar industry, and its output is second only to sugar cane. Cytoplasmic male sterility (CMS) is a phenomenon of pollen abortion that has important implications in sugar beet hybrid breeding. Male plant sterility is usually considered to be associated with mitochondrial dysfunction. Although mitochondrial genes associated with male sterility have been well explored, the different mitochondrial proteomics of CMS in sugar beet are still poorly understood. In this study, differentially expressed mitochondrial proteomic analysis was performed on the flower buds of the male sterile line (DY5-CMS), its maintainer line (DY5-O) and a fertility restorer line (CL6), using an isobaric tag for relative and absolute quantitation (iTRAQ) technology. A total of 2260 proteins were identified by mass spectrometry, of which 538 were differentially expressed proteins. Most of them were involved in protein metabolism, carbohydrate and energy metabolism, and binding. More specifically, some cysteine and methionine metabolism proteins (A0A0J8BGE0, A0A0J8CZM6, A0A0J8D7W0 and A0A0J8BCR7) may play important roles during the formation of CMS. This study provided an in–depth understanding of the CMS molecular mechanism at the protein level in sugar beet.

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