scholarly journals Determination of Sambucus Interspecific Hybrid Structure using Molecular Markers

Agricultura ◽  
2019 ◽  
Vol 16 (1-2) ◽  
pp. 1-10
Author(s):  
Metka Šiško ◽  
◽  
Anja Ivanuš ◽  
Anton Ivančič ◽  
◽  
...  
Keyword(s):  
Parental Species ◽  
Genetic Recombination ◽  
Hybrid Structure ◽  
Main Group ◽  
Broad Sense ◽  
F1 Hybrids ◽  
The Third ◽  
Complex Hybrid ◽  
Black Beauty

Phenotypic and genotypic variations within the genus Sambucus are limited. They could be efficiently increased by genetic recombination involving different species. The aim of the presented investigation was to assess the possibility of using molecular approach (i.e., microsattelites) in determination of unknown hybrid structures. The study involved 47 Sambucus genotypes (parental species and intespecific hybrids), and six microsatellite loci were analysed. The clustering method grouped the analysed genotypes into four main groups. The first main group involved two sub-groups: one with taxons and hybrids involving S. racemosa (in broad sense) and the other involving two botanical varieties of S. nigra. In the second main group, there was a sub-group involving hybrids between S. javanica and S. nigra, and a sub-group involving F1 hybrids between S. javanica and S. ebulus. The third main group contained a sub-group with hybrids between S. javanica and S. nigra ‘Black Beauty’, a sub-group with hybrids involving S. javanica, S. nigra and S. racemosa (miquelii), a sub-group with backcrosses S. javanica × (S. javanica × S. ebulus), and un unknown hybrid. The fourth main group included a subgroup with F1 hybrids S. javanica × S. ebulus, a sub-group involving various taxons of S. racemosa (in broad sense), and a sub-group involving hybrids between S. cerulea and S. javanica, with and without S. nigra. Our study shows that molecular analysis can be helpful in determining some of the unknown but simple interspecific hybrids of Sambucus. In the cases of complex hybrid combinations, the use of SSRs is most probably not the best solution.

Investigations on skeletal growth zones via bone scans as base of determination of optimal time for surgery in mandibular asymmetry

2000 ◽  
Vol 39 (05) ◽  
pp. 121-126 ◽  
Author(s):  
R. Werz ◽  
P. Reuland
Keyword(s):  
Bone Growth ◽  
Region Of Interest ◽  
Tracer Uptake ◽  
Optimal Time ◽  
Mandibular Bone ◽  
The Third ◽  
Mandibular Asymmetry ◽  
Bone Scans ◽  
Epiphyseal Plates

Summary Aim of the study was to find out wether there is a common stop of growth of mandibular bone, so that no individual determination of the optimal time for surgery in patients with asymmetric mandibular bone growth is needed. As there are no epiphyseal plates in the mandibular bone, stop of growth cannot be determined on X-ray films. Methods: Bone scans of 731 patients [687 patients (324 male, 363 female) under 39 y for exact determination of end of growth and 44 (21 male, 23 female) patients over 40 y for evaluation of nongrowth dependant differences in tracer uptake] were reviewed for the study. All the patients were examined 3 hours after injection of 99mTc-DPD. Tracer uptake was measured by region of interest technique in different points of the mandibular bone and in several epiphyseal plates of extremities. Results: Tracer uptake in different epiphyseal plates of the extremities shows strong variation with age and good correlation with reported data of bone growth and closure of the epiphyseal plates. The relative maximum of bone activity is smaller in mandibular bone than in epiphyseal plates, which show well defined peaks, ending at 15-18 years in females and at 18-21 years in males. In contrast, mandibular bone shows no well defined end of growing but a gradually reduction of bone activity which remains higher than bone activity in epiphyseal plates over several years. Conclusion: No well defined end of growth of mandibular bone exists. The optimal age for surgery of asymmetric mandibular bone growth is not before the middle of the third decade of life, bone scans performed earlier for determination of bone growth can be omitted. Bone scans performed at the middle of the third decade of life help to optimize the time of surgical intervention.

THE FACTORS OF CAREER SELF-DETERMINATION OF STUDENTS OF HIGHER EDUCATION INSTITUTIONS

2019 ◽  
Vol 7 (1) ◽  
pp. 9-20
Author(s):  
Inna Yeung
Keyword(s):  
Higher Education ◽  
Higher Education Institutions ◽  
Well Being ◽  
Young Person ◽  
Self Determination ◽  
Full Time ◽  
Actual Problem ◽  
The Third ◽  
The Right

Choice of profession is a social phenomenon that every person has to face in life. Numerous studies convince us that not only the well-being of a person depends on the chosen work, but also his attitude to himself and life in general, therefore, the right and timely professional choice is very important. Research about factors of career self-determination of students of higher education institutions in Ukraine shows that self-determination is an important factor in the socialization of young person, and the factors that determine students' career choices become an actual problem of nowadays. The present study involved full-time and part-time students of Institute of Philology and Mass Communications of Open International University of Human Development "Ukraine" in order to examine the factors of career self-determination of students of higher education institutions (N=189). Diagnostic factors of career self-determination of students studying in the third and fourth year were carried out using the author's questionnaire. Processing of obtained data was carried out using the Excel 2010 program; factorial and comparative analysis were applied. Results of the study showed that initial stage of career self-determination falls down on the third and fourth studying year at the university, when an image of future career and career orientations begin to form. At the same time, the content of career self-determination in this period is contradictory and uncertain, therefore, the implementation of pedagogical support of this process among students is effective.

Determination of clinical efficiency of deproteinized dialysate from blood of milk-fed calves after endoprosthetics in patients with ventral hernias

2020 ◽  
Vol 28 (3) ◽  
pp. 323-333
Author(s):  
Sergej V. Ivanov ◽  
Ilya S. Ivanov ◽  
Evgenij G. Obyedkov ◽  
Liliya P. Popova
Keyword(s):  
Abdominal Wall ◽  
Inflammatory Reaction ◽  
Inflammatory Process ◽  
Anterior Abdominal Wall ◽  
Dynamic Change ◽  
Main Group ◽  
Control Group ◽  
Surgical Department ◽  
Regeneration Stage

Aim. To study the influence of deproteinized dialysate from blood of milk-fed calves on the type of exudative discharge and dynamics of inflammatory reaction after hernia repair with plastics of the anterior abdominal wall with hernioendoprosthesis of polypropylene. Materials and Methods. The study involved 59 patients being on stationary treatment in the surgical department of Kursk Regional Clinical Hospital. Patients were hospitalized for the herniation of small or medium dimension. The patients were divided to two groups: the main (n=30) and control (n=29) groups. After endoprosthetics, the patients of the control group were given complex conservative treatment. The patients of the main group, besides standard treatment, were administered deproteinized dialysate from blood of milk-fed calves intravenously in drips 10 ml + 200 ml of 0.9% sodium chloride solution within 7 days. For cytological examination and determination of the type of cytograms, the traumatic discharge was collected and analyzed using the method of M.F. Kamaev and M.A. Palthsev. Results. Cytomorphometric examination was conducted on the third, fifth and seventh day after endoprosthetics with the aim of studying dynamic changes. Determination of cell composition and also of its changes characteristic of each studied period, was necessary for obtaining further information characterizing inflammatory process in the region of placement of the endoprosthesis. After endoprosthetics in patients who were administered hemodialysate, the inflammatory reaction was less pronounced than in patients who did not receive the preparation. This was associated with a more dynamic change of stages of the inflammatory process. In patients of the main group who received deproteinized dialysate, regenerative type of inflammation first appeared on the fifth day and made 6.9%; by the seventh day the share of patients with the regenerative type rose to 17.5%, while in the patients of the control group no regeneration stage was observed in both periods. Conclusion. Analysis of the efficiency of influence of hemodialysate on the inflammatory reaction in plastics of the anterior abdominal wall with polypropylene endoprosthesis evidences faster course of all stages of inflammation and reduction of its intensity by 10%.

Comparative assessment of informational content of the equations of F.P. Hadlock and the computer program of V.N. Demidov in determination of term of gestation and fetal weight in the III trimester of gestation

2017 ◽  
Author(s):  
E.A. Derkach , O.I. Guseva
Keyword(s):  
Computer Program ◽  
High Precision ◽  
Gestational Age ◽  
Computer Programs ◽  
Comparative Assessment ◽  
Fetal Weight ◽  
Informational Content ◽  
Third Trimester ◽  
The Third

Objectives: to compare the accuracy of equations F.P. Hadlock and computer programs by V.N. Demidov in determining gestational age and fetal weight in the third trimester of gestation. Materials: 328 patients in terms 36–42 weeks of gestation are examined. Ultrasonography was performed in 0–5 days prior to childbirth. Results: it is established that the average mistake in determination of term of pregnancy when using the equation of F.P. Hadlock made 12,5 days, the computer program of V.N. Demidov – 4,4 days (distinction 2,8 times). The mistake within 4 days, when using the equation of F.P. Hadlock has met on average in 23,1 % of observations, the computer program of V.N. Demidov — 65,9 % (difference in 2,9 times). The mistake more than 10 days, took place respectively in 51,7 and 8,2 % (distinction by 6,3 times). At a comparative assessment of size of a mistake in determination of fetal mass it is established that when using the equation of F.P. Hadlock it has averaged 281,0 g, at application of the computer program of V.N. Demidov — 182,5 g (distinction of 54 %). The small mistake in the mass of a fetus which isn't exceeding 200 g at application of the equation of F.P. Hadlock has met in 48,1 % of cases and the computer program of V.N. Demidov — 64,0 % (distinction of 33,1 %). The mistake exceeding 500 g has been stated in 18 % (F.P. Hadlock) and 4,3 % (V.N. Demidov) respectively (distinction 4,2 times). Conclusions: the computer program of V.N. Demidov has high precision in determination of term of a gestation and mass of a fetus in the III pregnancy.

Introduction

2018 ◽  
Author(s):  
Chakravarthi Ram-Prasad
Keyword(s):  
Philosophical Tradition ◽  
Phenomenological Tradition ◽  
The Third ◽  
Western Philosophical Tradition ◽  
The Subject ◽  
To Come

The Introduction outlines the various chapters. It then situates the question of ‘body’ in the modern Western philosophical tradition following Descartes, and argues that this leaves subsequent responses to come under one of three options: metaphysical dualism of body and subject; any anti-dualist reductionism; or the overcoming of the divide. Describing the Phenomenology of Merleau-Ponty as a potent example of the third strategy, the Introduction then suggests his philosophy will function as foil to the ecological phenomenology developed and presented in the book. Moreover, one approach within the Western Phenomenological tradition, of treating phenomenology as a methodology for the clarification of experience (rather than the means to the determination of an ontology of the subject) is compared to the approach in this book. Since classical India, while understanding dualism, did not confront the challenge of Descartes (for better or for worse), its treatment of body follows a different trajectory.

scholarly journals Sequencing an F1 hybrid of Silurus asotus and S. meridionalis enabled the assembly of high-quality parental genomes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Weitao Chen ◽  
Ming Zou ◽  
Yuefei Li ◽  
Shuli Zhu ◽  
Xinhui Li ◽  
...  
Keyword(s):  
De Novo ◽  
Parental Species ◽  
F1 Hybrids ◽  
Pelteobagrus Fulvidraco ◽  
F1 Hybrid ◽  
Short Reads ◽  
Final Assembly ◽  
Genome Complexity ◽  
Hybrid Genome ◽  
Silurus Asotus

AbstractGenome complexity such as heterozygosity may heavily influence its de novo assembly. Sequencing somatic cells of the F1 hybrids harboring two sets of genetic materials from both of the paternal and maternal species may avoid alleles discrimination during assembly. However, the feasibility of this strategy needs further assessments. We sequenced and assembled the genome of an F1 hybrid between Silurus asotus and S. meridionalis using the SequelII platform and Hi-C scaffolding technologies. More than 300 Gb raw data were generated, and the final assembly obtained 2344 scaffolds composed of 3017 contigs. The N50 length of scaffolds and contigs was 28.55 Mb and 7.49 Mb, respectively. Based on the mapping results of short reads generated for the paternal and maternal species, each of the 29 chromosomes originating from S. asotus and S. meridionalis was recognized. We recovered nearly 94% and 96% of the total length of S. asotus and S. meridionalis. BUSCO assessments and mapping analyses suggested that both genomes had high completeness and accuracy. Further analyses demonstrated the high collinearity between S. asotus, S. meridionalis, and the related Pelteobagrus fulvidraco. Comparison of the two genomes with that assembled only using the short reads from non-hybrid parental species detected a small portion of sequences that may be incorrectly assigned to the different species. We supposed that at least part of these situations may have resulted from mitotic recombination. The strategy of sequencing the F1 hybrid genome can recover the vast majority of the parental genomes and may improve the assembly of complex genomes.

scholarly journals Electrical stimulation to clinically identify position of the lingual nerve: results of 50 subjects with reliability and correlation with MRI

2021 ◽  
Author(s):  
Sanaa Aljamani ◽  
Callum Youngson ◽  
Fadi Jarad ◽  
Francis O’Neill
Keyword(s):  
Third Molar ◽  
Lingual Nerve ◽  
Observer Agreement ◽  
Electrical Nerve Stimulation ◽  
The Third ◽  
Mri Scans ◽  
Tingling Sensation ◽  
Magnetic Resonance Imaging Mri ◽  
Clinical Determination

Abstract Purpose Recently we described mapping of the lingual nerve clinically in patients using electrical nerve stimulation. This paper reports results of a larger study with inter- and intra-observer reliability and comparison with positional measurements from magnetic resonance imaging (MRI). Methods In 50 healthy participants, measurements were taken when subjects felt a tingling sensation in the tongue induced by a stimulation probe over the lingual nerve. Three positions were measured in relation to the third molar. Measurement reliability was tested for both inter-observer and intra-observer agreement and positional data of the lingual nerve measured clinically was also compared with nerve position as measured from MRI scans. Results Out of 50 participants, 96 nerves (49 = left/47 = right) were included in the study. The lingual nerve was identified in 90% (87) of this sample. The mean of height of the nerve in points A, B and C were 9.64 mm, 10.77 mm and 12.34 respectively. Inter-and intra-observer agreement was considered to be good to excellent (ICC = 0.8–0.96). Agreement between nerve mapping measured values and MRI measured values was good (ICC < 0.6). Conclusion This technique may prove useful for the clinical determination of lingual nerve position prior to procedures in the third molar region.

scholarly journals Three Different Spectrophotometric Methods for Simultaneous Determination of Pyriproxyfen and Chlorothalonil Residues in Cucumber and Cabbage Samples

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Shilan A. Omer ◽  
Nabil A. Fakhre
Keyword(s):  
Simultaneous Determination ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
The Third ◽  
Mean Centering ◽  
Relative Standard ◽  
One Way Anova

In this study, three simple and accurate spectrophotometric methods for simultaneous determination of pyriproxyfen and chlorothalonil residues in cucumbers and cabbages grown in experimental greenhouse were studied. The first method was based on the zero-crossing technique measurement for first and second derivative spectrophotometry. The second method was based on the first derivative of the ratio spectra. However, the third method was based on mean centering of ratio spectra. These procedures lack any previous separation steps. The calibration curves for three spectrophotometric methods are linear in the concentration range of 1–30 μg·mL−1 and 0.5–7 μg·mL−1 for pyriproxyfen and chlorothalonil successively. The recoveries ranged from 82.12–97.40% for pyriproxyfen and 81.51–97.04% for chlorothalonil with relative standard deviations less than 4.95% and 5.45% in all instances for pyriproxyfen and chlorothalonil, respectively. The results obtained from the proposed methods were compared statistically by using one-way ANOVA, and the results revealed there were no significant differences between ratio spectra and mean centering methods with the zero-crossing technique. The proposed methods are successfully applied for the simultaneous estimation of the residue of both pesticides in cucumber and cabbage samples.

Quantitative Bestimmung der hydrierten Pterine und des Xanthommatins in den Augen von Ceratitis capitata WIED (Dipt. Trypetidae)

1965 ◽  
Vol 20 (4) ◽  
pp. 318-322 ◽  
Author(s):  
Irmgard Ziegler ◽  
M. Feron
Keyword(s):  
Quantitative Determination ◽  
Ceratitis Capitata ◽  
Male And Female ◽  
The Third ◽  
Quantitative Bestimmung ◽  
Drastic Increase

In the eyes of male and female flies a quantitative determination of the tetrahydropterin, the corresponding dihydro-compound (Sepiapterin) and of xanthommatin from 0—5 days after hatching was made. The increase of xanthommatin almost stops 24 hours after hatching; about 13 μg are present after that time. The tetrahydropterin, after a drastic increase during the first 24 hours, remains at a level of about 1,1 — 1,2 µg. In contrast the dihydrocompound which is nearly absent at the time of hatching is deposited on the eye-granules between the third and fifth day. At that time, especially in the male the dihydropterin/tetrahydropterin ratio is increased. Starvation during the first 24 hours has essentially no effect on the amount of xanthommation whereas the amount of pterins is markedly reduced.

Determination of Instability Boundaries for a Highly Flexible Prismatic-Jointed Robot Performing Repetitive Maneuvers

1991 ◽  
Author(s):  
Keith W. Buffinton
Keyword(s):  
Floquet Theory ◽  
Equations Of Motion ◽  
Perturbation Techniques ◽  
Axial Motion ◽  
The Third ◽  
Straightforward Application ◽  
Method Yield ◽  
Robotic Devices ◽  
Axially Moving

Abstract Presented in this work are the equations of motion governing the behavior of a simple, highly flexible, prismatic-jointed robotic manipulator performing repetitive maneuvers. The robot is modeled as a uniform cantilever beam that is subject to harmonic axial motions over a single bilateral support. To conveniently and accurately predict motions that lead to unstable behavior, three methods are investigated for determining the boundaries of unstable regions in the parameter space defined by the amplitude and frequency of axial motion. The first method is based on a straightforward application of Floquet theory; the second makes use of the results of a perturbation analysis; and the third employs Bolotin’s infinite determinate method. Results indicate that both perturbation techniques and Bolotin’s method yield acceptably accurate results for only very small amplitudes of axial motion and that a direct application of Floquet theory, while computational expensive, is the most reliable way to ensure that all instability boundaries are correctly represented. These results are particularly relevant to the study of prismatic-jointed robotic devices that experience amplitudes of periodic motion that are a significant percentage of the length of the axially moving member.

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