PCR Analysis of Genes from Aeromonas punctata for Reactive Black 5 Dye Degradation

In recent years, groundwater contamination caused by dyes has become an important problem. They enter into wastewater as a result of the textile, automotive, or cosmetics industries. For this reason, new methods are being sought, which would aid at the removal of dye impurities with high efficiency and also would be relatively cheap. In the presented study synthesized TiO2-ZrO2 (with TiO2:ZrO2 molar ratio of 8:2) and TiO2-ZrO2-SiO2 (with TiO2:ZrO2:SiO2 molar ratio of 8:1:1) oxide materials were used as supports for enzyme immobilization. Effective synthesis of the carriers was confirmed by results of scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), low-temperature nitrogen sorption and Fourier transform infrared spectroscopy (FTIR). The materials achieve high immobilization efficiency of the laccases from Trametes versicolor (83% and 96% for TiO2-ZrO2-laccase and TiO2-ZrO2-SiO2-laccase, respectively). The effect of selected dye concentrations, pH, temperature, and reusability were also tested. The obtained results showed that after removal of textile dyes, such as Alizarin Red S (ARS), Remazol Brilliant Blue R (RBBR), and Reactive Black 5 (RB5), under optimal process conditions, which were pH 5 and 25 °C, from dye solution of 5 mg/L degradation efficiency reached 100%, 91%, and 77%, respectively, suggesting synergistic mechanism of degradation by simultaneous sorption and catalytic action. Finally, reduction of chemical oxygen demand (COD) of the solution after treatment indicated lower mixture toxicity and effective dye degradation.

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Investigating degradation metabolites and underlying pathway of azo dye “Reactive Black 5” in bioaugmented floating treatment wetlands

10.21203/rs.3.rs-351058/v1 ◽

2021 ◽

Author(s):

Nain Tara ◽

Mazhar Iqbal ◽

Fazale Habib ◽

Qaiser Mahmood Khan ◽

Samina Iqbal ◽

...

Keyword(s):

Azo Dyes ◽

Azo Dye ◽

Dye Degradation ◽

Treatment Wetlands ◽

Reactive Black 5 ◽

Acinetobacter Junii ◽

Degrading Bacteria ◽

Carcinogenic Effects ◽

Successful Removal ◽

Floating Treatment Wetlands

Abstract The direct discharge of azo dyes and/or their metabolites into the environment may exert toxic, mutagenic, and carcinogenic effects on exposed fauna and flora. In this study, we analyzed the metabolites produced during the degradation of an azo dye namely Reactive Black 5 (RB5) in the bacterial augmented-floating treatment wetlands (FTWs), followed by the investigation of their underlying toxicity. To this end, a FTWs system was developed by using a common wetland plant Phragmites australis in the presence of three dye-degrading bacteria (Acinetobacter junii strain NT-15, Pseudomonas indoloxydans strain NT-38, and Rhodococcus sp. strain NT-39). We found that the FTW system effectively degraded RB5 into at least 20 different metabolites with the successful removal of color (95.5%) from the water. The fish toxicity assay revealed the non-toxic characteristics of the metabolites produced after dye degradation. Our study suggests that bacterially aided FTWs could be a suitable option for the successful degradation of azo dyes, and the results presented in this study may help improve the overall textile effluent clean-up processes.

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Degradation of Reactive Dyes by Thermally Activated Persulfate and Reuse of Treated Textile Dye-Bath

Journal of the Brazilian Chemical Society ◽

10.21577/0103-5053.20210077 ◽

2021 ◽

Author(s):

Amanda Basilio ◽

Lucas Dohler ◽

Matheus Servin ◽

Carlos Gouvea ◽

Ronny Ribeiro ◽

...

Keyword(s):

Textile Industry ◽

Dye Degradation ◽

Textile Dyes ◽

Color Removal ◽

Textile Dye ◽

Reactive Black 5 ◽

Thermally Activated ◽

Good Potential ◽

High Concentrations ◽

Activated Persulfate

Due to the usual resistance of textile dyes to conventional biological treatment processes, the color removal of dyeing wastewaters remains a challenge for the textile industry. This work evaluates the capacity of advanced oxidation processes based on thermally-activated persulfate concerning textile dyes’ degradation in aqueous solution and the reuse of dyeing baths. Preliminary studies were carried out in a bench-scale jacketed reactor, using Reactive Black 5 (40 mg L-1) as a model dye. Almost complete dye degradation was observed in 60 min in this stage, using 300 mg L-1 of persulfate and activation temperatures of 80 ºC, basically due to the action of radical sulfate. The use of high concentrations of persulfate (1000 mg L-1) allowed efficient color removal of dyeing baths containing remazol dyes in processes activated at 80 and 90 ºC. However, the wastewaters treated under these conditions did not lead to high dyeing efficiency in reuse studies, probably due to residual persulfate presence. In contrast, low concentrations of persulfate (250 mg L-1) lead to partial color removal and a better dyeing quality. The results suggest a good potential for treating high-temperature dyeing baths, saving water and auxiliary agents used in textile dyeing processes.

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Water Contaminated by Industrial Textile Dye: Study on Decolorization Process

Environments ◽

10.3390/environments6090101 ◽

2019 ◽

Vol 6 (9) ◽

pp. 101 ◽

Cited By ~ 2

Author(s):

Pierantonio De Luca ◽

Paola Foglia ◽

Carlo Siciliano ◽

Jànos B. Nagy ◽

Anastasia Macario

Keyword(s):

Hydrogen Peroxide ◽

Titanium Oxide ◽

Sodium Carbonate ◽

Dye Degradation ◽

Reaction Rates ◽

Textile Dye ◽

Reactive Black 5 ◽

Experimental Conditions ◽

Textile Industries

This work aims to investigate possible interferences due to the presence of sodium carbonate on the photodegradation of the reactive Black 5 azoic dye, both in systems containing only titanium oxide and those containing titanium oxide and hydrogen peroxide. The role of hydrogen peroxide is explicitly treated. Sodium carbonate, in fact, is often present in the wastewater of textile industries as it is used in the fiber dyeing phases. The use of TiO2 nanoparticles is emphasized, and the possible danger is underlined. Each system was subjected to ultraviolet irradiation (UV) by varying the exposure time. After the photodegrading tests, the resulting solutions were analyzed by UV-vis spectrophotometry and High-Resolution Nuclear Magnetic Resonance to measure the residual concentrations of dye. The dye degradation curves and reaction rates for different UV exposure times were obtained and discussed as a function of the used additives. All the data are repeated three times, and they differ only by a maximum of 5%. The results indicated a reduction of about 50% of the initial concentration of Reactive Black 5 after 30 min under optimal experimental conditions. The NMR analysis indicated the formation of a series of aromatic structures that were generated by the UV-induced photochemical fragmentation of the original molecule.

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Effects of the Addition of Fe, Co on the Azo Dye Degradation Ability of Mn-Al Mechanically Alloyed Powders

Metals ◽

10.3390/met10121578 ◽

2020 ◽

Vol 10 (12) ◽

pp. 1578

Author(s):

Wael Ben Mbarek ◽

Joan Saurina ◽

Lluisa Escoda ◽

Eloi Pineda ◽

Mohamed Khitouni ◽

...

Keyword(s):

High Performance ◽

Dye Degradation ◽

Research Work ◽

Azo Compounds ◽

Reactive Black 5 ◽

Leather Industry ◽

Mechanically Alloyed ◽

Visible Absorption ◽

X Ray ◽

Co Addition

Azo compounds are used in the textile and leather industry. A significant step during the azo dyes treatment of water is the degradation by breaking the N=N bonds. This break produces the decolorization of water. In this research work, 10% atomic of Fe or Co was added to produce ternary Mn-Al-rich, nanostructured, mechanically alloyed powders in order to improve the decolorization of Reactive Black 5 solutions and to check Fe and Co addition’s influence. The microstructure was followed by X-ray diffraction, the morphology and composition by electronic microscopy and energy-dispersive X-ray spectroscopy (EDS) microanalysis. The dye degradation was monitored with ultraviolet/visible absorption spectrophotometry. After degradation, the remaining organic compound was checked by high-performance liquid chromatography (HPLC) and the functional groups of the powdered alloys by infrared spectroscopy. Fe addition to Mn-Al displayed faster kinetics and a higher efficiency than the Co addition. The Mn-Al-Fe solution (0.25 g/100 mL) was fully decolorized in 5 min. On the other side, Mn-Al-Co powders were able to successfully decolorize the dyed solution in 10 min under the same conditions. Thus, nanocrystalline Fe-doped Mn-Al alloys are good candidates for use in the decolorization process, in comparison with Co-doped and other intermetallic particles.

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Screening of wild basidiomycetes and evaluation of the biodegradation potential of dyes and lignin by manganese peroxidases

BioResources ◽

10.15376/biores.14.3.6558-6576 ◽

2019 ◽

Vol 14 (3) ◽

pp. 6558-6576

Author(s):

Ramya G. Rao ◽

Aarthi Ravichandran ◽

Giridhar Kandalam ◽

Samanta Ashish Kumar ◽

Senani Swaraj ◽

...

Keyword(s):

Dye Degradation ◽

White Rot Fungi ◽

Bromothymol Blue ◽

Kraft Lignin ◽

White Rot ◽

Bromocresol Green ◽

Reactive Black 5 ◽

Ftir Analysis ◽

The Fourier Transform ◽

Almost All

Manganese peroxidase (MnP), a crucial enzyme in biodegradation of lignin, is synthesized by most white rot fungi. To obtain novel enzymes with superior biodegradation potential, MnP-producing wild isolates were evaluated for their ability to degrade recalcitrant azo dyes, sulfonephthalein dyes, and kraft lignin. Of 30 wild isolates screened, 18 tested positive for lignin modifying enzymes (LMEs). Thirteen of these isolates were positive for both laccase and MnP, whereas four produced only laccase, and one produced lignin peroxidase alone. The isolates were identified as Clitopilus scyphoides MH172162 (AGUM004), Ganoderma rasinaceum MH172163 (AGUM007), and three Schizophyllum species: MH172164, MH172165, and MH172166 (KONA001, AGUM0011, and AGUM021). The Fourier-transform infrared spectroscopy (FTIR) analysis of dye degradation and kraft lignin samples with AGUM004 and AGUM007 revealed biotransformation. The former could not completely degrade Reactive Black 5 and bromocresol green, but it could completely (100%) decolorize bromophenyl blue, bromothymol blue, and Remazol brilliant blue R. The latter efficiently degraded almost all tested dyes. Both degraded kraft lignin. The screened hyper MnP-producing wild AGUM004 and AGUM007 were shown to be potential dye degraders in addition to having lignin degrading abilities.

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Decolorization and Detoxification of Synthetic Dyes by Mexican Strains of Trametes sp.

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16234610 ◽

2019 ◽

Vol 16 (23) ◽

pp. 4610

Author(s):

Levin ◽

Hernández-Luna ◽

Niño-Medina ◽

García-Rodríguez ◽

López-Sadin ◽

...

Keyword(s):

Raphanus Sativus ◽

Dye Degradation ◽

Degradation Products ◽

Ligninolytic Enzyme ◽

Reactive Black 5 ◽

Synthetic Dyes ◽

Redox Mediators ◽

Liquid Media ◽

Solid Media ◽

Mexican Strains

Laccases have attracted a great deal of interest because of their remarkable ability for the degradation of synthetic dyes present in wastewaters. New laccase producing sources with robust operational and functional properties are being continuously explored. In this work, the potential for the decolorization and detoxification of synthetic dyes was evaluated in two Mexican strains of the genus Trametes. The decolorization capacity of Trametes maxima LE130 and Trametes sp. LA1 was tested in solid and liquid media. The phytotoxicity of the degradation products was determined using Raphanus sativus and Pisum sativum seeds. In solid media, both strains showed a higher decolorization capacity (p ≤ 0.05) than Phanerochaete chrysosporium ATCC 24725, which is known to be very efficient in lignin and dye-degradation. They produced laccase as the main ligninolytic enzyme; T. maxima LE130 secreted a single isoform of 43.9 kDa, while Trametes sp. LA1 produced three isoforms of 67.3, 58.6 and 52.7 kDa, respectively. Trametes sp. LA1 culture fluids were capable of decolorizing and detoxifying chemically diverse dyes (anthraquinonic dye Remazol Brilliant Blue R, azoic Reactive Black 5 and triphenylmethane Crystal Violet) without the addition of redox mediators. Therefore, this could be considered as a new laccase source which could be potentially competitive in the bioremediation of dye-containing wastewaters.

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Effective Dye Degradation by Graphene Oxide Supported Manganese Oxide

Processes ◽

10.3390/pr7010040 ◽

2019 ◽

Vol 7 (1) ◽

pp. 40 ◽

Cited By ~ 16

Author(s):

Hayarpi Saroyan ◽

George Kyzas ◽

Eleni Deliyanni

Keyword(s):

Graphene Oxide ◽

Catalytic Activity ◽

Surface Chemistry ◽

Manganese Oxide ◽

Azo Dye ◽

Dye Degradation ◽

High Catalytic Activity ◽

Reactive Black 5 ◽

Ambient Conditions ◽

Nanocomposite Catalyst

Graphene oxide (GO) was used as a support for manganese oxide (MnO2) for the preparation of a nanocomposite catalyst for the degradation of an azo dye, Reactive Black 5 (RB5). The nanocomposite was characterized for the structure by XRD, for the morphology with SEM, and for the surface chemistry with FTIR and potentiometric titration measurements. The GO-MnO2 nanocomposite presented a high catalytic activity for the degradation/oxidation of RB5 at ambient conditions, which was higher than that of the pure MnO2 and could be attributed to the beneficial contribution of the manganese oxide and the graphene oxide.

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Reactive Black 5 dye degradation using filters of smuggled cigarette modified with Fe3+

Environmental Science and Pollution Research ◽

10.1007/s11356-016-6820-0 ◽

2016 ◽

Vol 24 (7) ◽

pp. 6143-6150 ◽

Cited By ~ 6

Author(s):

Letícia Polli Glugoski ◽

Paloma de Jesus Cubas ◽

Sérgio Toshio Fujiwara

Keyword(s):

Dye Degradation ◽

Reactive Black 5

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