Role of Polymeric Additives in Formulation, Shelf-life and Bioefficacy of Liquid Inoculant of Pseudomonas fluorescens

2018 ◽  
Vol 6 (4) ◽  
pp. 123-133
Author(s):  
B. J. Praveen Biradar ◽  
Keyword(s):  
Shelf Life ◽  
Pseudomonas Fluorescens ◽  
Polymeric Additives ◽  
Liquid Inoculant

scholarly journals Role of endomycorrhizae and Pseudomonas fluorescens on the acclimatization of micropropagated Stevia rebaudiana Bert. plantlets

2017 ◽  
Vol 11 (3) ◽  
pp. 38-47
Author(s):  
S. Zayed Mona ◽  
Abd El-Moneim Hegazi Ghada ◽  
Mohammed Salem Hanaa ◽  
Mohammed Ibrahim Ali Adas Walaa
Keyword(s):  
Pseudomonas Fluorescens ◽  
Stevia Rebaudiana

scholarly journals THE ROLE OF POTASSIUM AND CALSIUM IN IMPROVING THE QUALITY AND SHELF LIFE OF TOMATO (Lycopersicum esculentum var. servo)

el–Hayah ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 84-93
Author(s):  
Puguh Catur Wicaksana ◽  
Ketut Anom Wijaya ◽  
Sigit Soeparjono
Keyword(s):  
Cell Wall ◽  
Shelf Life ◽  
Cell Wall Regeneration ◽  
Cross Link ◽  
Tomato Production ◽  
High Productivity ◽  
Long Duration ◽  
High Respiration Rate ◽  
Quality And Shelf Life

Tomato has the characteristics natural to damage. Damage fruits are caused by destitute of fruit quality and sensory attributes. The loss of tomato production on harvest in developing countries reached 50% due to the long duration of tomato distribution. Servo varieties of tomatoes are in demand by farmers because of their genetic advantages, high productivity, and abundant fruit weights. The shortage of servo varieties of tomatoes is the short shelf life of fruit, which is seven days after harvest. Short shelf life is influenced by fruit thickness and fruit hardness. In improving the quality and shelf life of tomatoes, potassium (K) nutrition plays a role in photosynthetic translocation, strengthens the cell wall, and involved in the lignification process of sclerenchyma tissue. Tomatoes, as climacteric fruits have a high respiration rate influenced by ethylene. Ethylene production can be inhibited by calcium chloride (CaCl2) application by strengthening the cell wall mechanism, cross-link with pectin, and increase the cell wall regeneration can change the texture of the fruit become harder, and it reduces sensitivity to physiological damage. Based on the treatment of this research, the quality and shelf life of servo varieties of tomato is increased.

scholarly journals Increased Fitness of Pseudomonas fluorescens Pf0-1 Leucine Auxotrophs in Soil

2008 ◽  
Vol 74 (12) ◽  
pp. 3644-3651 ◽  
Author(s):  
Wook Kim ◽  
Stuart B. Levy
Keyword(s):  
Pseudomonas Fluorescens ◽  
Bacterial Genome ◽  
Physiological Role ◽  
Underlying Mechanism ◽  
Growth Conditions ◽  
Soil Environment ◽  
Fitness Advantage ◽  
Gene Structures

ABSTRACT The annotation process of a newly sequenced bacterial genome is largely based on algorithms derived from databases of previously defined RNA and protein-encoding gene structures. This process generally excludes the possibility that the two strands of a given stretch of DNA can each harbor a gene in an overlapping manner. While the presence of such structures in eukaryotic genomes is considered to be relatively common, their counterparts in prokaryotic genomes are just beginning to be recognized. Application of an in vivo expression technology has previously identified 22 discrete genetic loci in Pseudomonas fluorescens Pf0-1 that were specifically activated in the soil environment, of which 10 were present in an antisense orientation relative to previously annotated genes. This observation led to the hypothesis that the physiological role of overlapping genetic structures may be relevant to growth conditions outside artificial laboratory media. Here, we examined the role of one of the overlapping gene pairs, iiv19 and leuA2, in soil. Although iiv19 was previously demonstrated to be preferentially activated in the soil environment, its absence did not alter the ability of P. fluorescens to colonize or survive in soil. Surprisingly, the absence of the leuA2 gene conferred a fitness advantage in the soil environment when leucine was supplied exogenously. This effect was determined to be independent of the iiv19 gene, and further analyses revealed that amino acid antagonism was the underlying mechanism behind the observed fitness advantage of the bacterium in soil. Our findings provide a potential mechanism for the frequent occurrence of auxotrophic mutants of Pseudomonas spp. in the lungs of cystic fibrosis patients.

Application of the sensory evaluation method when using the accelerated storage technique

2021 ◽  
pp. 15-17
Author(s):  
Елена Анатольевна Юрова ◽  
Татьяна Викторовна Кобзева
Keyword(s):  
Shelf Life ◽  
Evaluation Method ◽  
Main Task ◽  
Long Term Storage ◽  
Organoleptic Characteristics ◽  
Accelerated Storage ◽  
Shelf Stable

Основной задачей для обеспечения качества функциональных продуктов длительного хранения является наличие методов оценки срока годности, применение которых позволит оценить не только качество продукта, но и его функциональные свойства на протяжении всего срока годности продукта. В настоящее время установлена возможность разработки методики ускоренного хранения для функциональных продуктов на молочной основе длительного хранения с применением метода ASLT и математического моделирования, исходя из имеющихся наработанных статистических данных по показателям окислительной порчи, содержанию свободных аминокислот и параметрам оценки продуктов гидролиза белка. В данной работе приведена оценка роли органолептического анализа при подтверждении срока годности продукции, рассмотрены основные процессы, влияющие на изменение органолептических характеристик при хранении. По результатам выполненной работы даны рекомендации по использованию метода органолептической оценки применительно к функциональным продуктам на молочной основе при использовании методики ускоренного хранения. The main task for ensuring the quality of functional shelf-stable products is the availability of methods for assessing the shelf life, the use of which will allow not only assessing the quality of the product, but also its functional properties throughout the entire shelf life of the product. Currently, the possibility of developing a method of accelerated storage for functional milk-based products for long-term storage using the ASLT method and mathematical modeling has been established, based on the available accumulated statistical data on indicators of oxidative spoilage, the content of free amino acids and parameters for evaluating protein hydrolysis products. This work evaluates the role of organoleptic analysis in confirming the shelf life of products, considers the main processes that affect the change in organoleptic characteristics during storage. Based on the results of the work performed, recommendations were given on the use of the method of organoleptic assessment in relation to functional milk-based products when using the accelerated storage method.

Molecular Analysis of Spoilage-Related Bacteria in Pasteurized Milk during Refrigeration by PCR and Denaturing Gradient Gel Electrophoresis

2009 ◽  
Vol 72 (3) ◽  
pp. 572-577 ◽  
Author(s):  
HONGFEI HE ◽  
JIN DONG ◽  
CHIN NYEAN LEE ◽  
YONG LI
Keyword(s):  
Shelf Life ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Gradient Gel Electrophoresis ◽  
Aerobic Plate Count ◽  
Species Specific

Bacterial diversity in fluid milk products has been extensively studied in order to improve milk quality. Here, we illustrate the utility of viable counts and PCR–denaturing gradient gel electrophoresis (DGGE) for monitoring the microbial spoilage of pasteurized milk during shelf life. Five pasteurized milk samples stored at 4°C were examined at 10 and 5 days before expiration and on the expiration day. With bacterial DNA extracted directly from the samples, PCR-DGGE analysis indicated that Pseudomonas became dominant in four samples. Meanwhile, the aerobic plate count of these four samples exceeded the regulatory limit of 20,000 CFU/ml at 5 days before expiration, and the rapid psychrotrophic count markedly surpassed the aerobic plate count on the expiration day. Streptococcus and Buttiauxella spp. were detected in several samples. Sequence analysis of DGGE fragments revealed high diversity among Pseudomonas spp. in the milk samples. P. putida and P. migulae grew to high numbers during refrigerated storage. Further identification of Pseudomonas at the species level was facilitated by PCR and multiplex PCR using species-specific primers; consequently, P. fluorescens and P. fragi were observed. These results highlight an important role of Pseudomonas in the shelf life of pasteurized milk.

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