scholarly journals ​​In vitro Evaluation and Methane Production Potential of Rice Gluten Meal based Concentrate Feeds in Buffalo Inoculum

2021 ◽  
Author(s):  
Manpreet . ◽  
Jasmine Kaur ◽  
J.S. Lamba ◽  
R.S. Grewal
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Relative Proportion ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Total Carbohydrate ◽  
In Vitro Evaluation ◽  
Acid Detergent Fibre

Background: There is shortage of conventional feedstuffs to feed livestock in the country. Thus, this study was intended to evaluate the chemical composition and in vitro nutrient utilization of concentrate feeds containing graded levels of rice gluten meal (RGM). Methods: Five concentrate feeds containing graded levels of RGM at 0, 6.25, 12.50, 18.75 and 25% replacing soybean meal @ 0, 25, 50, 75 and 100% on w/w basis were prepared and designated as conc-1, conc-2, conc-3,conc-4 and conc-5. In vitro evaluation of feeds was carried out by in vitro gas production technique. Data were analysed by one way ANOVA using SPSS (2012) version 21. Result: The total ash, acid detergent fibre (ADF) and acid detergent lignin (ADL) content decreased while organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF), hemicellulose, acid detergent insoluble crude protein (ADICP), neutral detergent insoluble crude protein (NDICP) and total carbohydrate (TCHO) content increased with increase in the inclusion of RGM in the concentrate feeds. The OM digestibility was lower (P less than 0.05) in concentrate feeds 3, 4 and 5 than concentrate feed 1 and 2. Microbial mass production (MMP), efficiency of MMP and ME availability were not affected by including RGM in feeds. NH3-N showed a declining trend with increasing level of RGM in concentrate feeds. The relative proportion of acetate decreased (P less than 0.05) while that of propionate increased (P less than 0.05) with increasing level of RGM in the concentrate feeds. Methane production varied non-significantly among the concentrate feeds. The fermentation efficiency was higher (P less than 0.05) in concentrate 4 and 5 than other concentrates.

scholarly journals In Vitro Fermentation Characteristics and Methane Mitigation Responded to Flavonoid Extract Levels from Alternanthera sissoo and Dietary Ratios

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 109
Author(s):  
Sukruthai Sommai ◽  
Anusorn Cherdthong ◽  
Chanon Suntara ◽  
Sarong So ◽  
Metha Wanapat ◽  
...  
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Acid Detergent Fiber ◽  
In Vitro Fermentation

Two experiments were conducted under this study: Experiment 1 was to study production yield, chemical composition, and in vitro degradability of Brazilian spinach (Alternanthera sissoo; BS) leaf and leaf + leaf-stalk at various maturity ages of 15, 30, 45, and 60 days after plantation and regrowth and Experiment 2 was to evaluate the effect of flavonoid extract from BS leaf and leaf + leaf-stalk and dietary ratios on ruminal gas production, fermentation characteristics, and in vitro degradability. Experiment 1 showed that maturity ages after planting and regrowth increased, the yield significantly increased. Increasing maturity ages significantly (p < 0.05) increased neutral detergent fiber and acid detergent fiber content and decreased crude protein content, total flavonoid (TF) content, and degradability for both leaf and leaf + leaf-stalk. Maturity ages from 15 to 30 days after plantation and regrowth resulted (p < 0.05) the highest TF content and degradability for both leaf and leaf + leaf-stalk. Thus, BS leaf and leaf + leaf-stalk samples from 15 to 30 days of age were used for flavonoid extraction and used in the Experiment 2. Experiment 2 was conducted according to a 3 × 5 factorial experiment. Three roughage to concentrate (R:C) ratios at 50:50, 40:60, and 30:70 were used, and five levels of flavonoid extract (FE) at 0, 10, 20, 30, and 40 mg of substrate dry matter (DM) were supplemented. Experiment 2 showed that R:C ratio and FE had an interaction effect only on acetate to propionate ratio. Varying R:C ratios significantly increased (p < 0.05) in vitro DM degradability, total volatile fatty acids (VFA), and propionate (C3) concentration. FE supplementation linearly (p < 0.05) increased total VFA and C3 concentration and decreased methane production and protozoal population. This study could conclude that FE from BS could effectively modulate ruminal fermentation and decrease methane production. However, in vivo study needs to elucidate in order to validate the present results.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

Essential oils from Lippia turbinata and Tagetes minuta persistently reduce in vitro ruminal methane production in a continuous-culture system

2019 ◽  
Vol 59 (4) ◽  
pp. 709 ◽  
Author(s):  
F. Garcia ◽  
P. E. Vercoe ◽  
M. J. Martínez ◽  
Z. Durmic ◽  
M. A. Brunetti ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Essential Oils ◽  
Acid Concentration ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Crude Protein ◽  
Gas Production ◽  
Volatile Fatty Acid Concentration ◽  
Tagetes Minuta

The aim of the present study was to evaluate the impact of essential oils (EO) from Lippia turbinata (LT) and Tagetes minuta (TM) as well as the rotation of both EO on fermentation parameters in vitro. Daily addition of LT, TM, or a 3-day rotation between them (TM/LT), as well as a control (without EO), was evaluated using the rumen simulation technique (Rusitec). The experiment lasted 19 days, with a 7-day adaptation period, followed by 12 days of treatment (Days 0–12). The EO were dissolved in ethanol (70% vol/vol) to be added daily to fermenters (300 μL/L) from Day 0. Daily measurements included methane concentration, total gas production, apparent DM disappearance and pH, which started 2 days before the addition of treatments. On Days 0, 4, 8 and 12 apparent crude protein disappearance and neutral detergent fibre disappearance, ammonia and volatile fatty acid concentration and composition were determined. Methane production was significantly inhibited shortly after addition of both EO added individually, and persisted over time with no apparent adaptation to EO addition. The TM/LT treatment showed a similar effect on methane production, suggesting that rotating the EO did not bring further improvements in reduction or persistency compared with the inclusion of the EO individually. Gas production, total volatile fatty acid concentration and composition and apparent crude protein disappearance were not affected by EO addition. Compared with the control, a 5% reduction of apparent DM disappearance and a 15% reduction of neutral detergent fibre disappearance were observed with the addition of EO. Only TM and TM/LT reduced ammonia concentration. Given the significant and persistent antimethanogenic activity of both EO, and the potential of T. minuta to modify nitrogen metabolism, EO from these plant species are of interest for developing new feed additives with potential application in ruminant nutrition that are also likely to be acceptable to consumers.

scholarly journals In vitro fermentation, digestibility and methane production of tropical perennial grass species

2014 ◽  
Vol 65 (5) ◽  
pp. 479 ◽  
Author(s):  
Belete Shenkute Gemeda ◽  
Abubeker Hassen
Keyword(s):  
Organic Matter ◽  
Methane Production ◽  
Rumen Fluid ◽  
Perennial Grass ◽  
Gas Production ◽  
Automated System ◽  
Grass Species ◽  
Tropical Conditions ◽  
Acid Detergent Fibre

This study characterised 16 tropical perennial grass species in terms of in vitro methane output and related their digestibility and rumen fermentation with methane output. The grass samples were collected, dried in a forced oven, and ground and analysed for nutrient composition. In vitro gas production and organic matter digestibility (IVOMD) were determined using rumen fluid collected, strained and anaerobically prepared. A semi-automated system was used to measure gas production through in vitro incubation at 39°C. Anthephora argentea and Stipagrostis ciliate produced the highest concentration of methane in terms of g kg–1 digestible dry matter (DDM) and g kg–1 digestible organic matter (IVOMD). Cenchrus ciliaris, Setaria verticillata and Panicum coloratum produced the lowest (P < 0.05) methane when expressed in terms of g kg–1 DDM and g kg–1 IVOMD. Ash, ether extract, non-fibrous carbohydrate, neutral and acid detergent insoluble nitrogen, and crude protein were negatively correlated with methane production. Methane production positively correlated with neutral and acid detergent fibre, cellulose and hemicellulose. It is important to focus on screening and selecting perennial grass with higher nitrogen content and low methane production to mitigate methane production under tropical conditions.

Nutritional value of Phalaris minor seeds and its comparison with conventional cereal grains for livestock feeding

2017 ◽  
Author(s):  
Jasmine Kaur ◽  
S. Thakur ◽  
Mahendra Singh
Keyword(s):  
Crude Protein ◽  
Nutritional Value ◽  
Gas Production ◽  
Cereal Grains ◽  
Phalaris Minor ◽  
Acid Detergent Lignin ◽  
Mineral Profile ◽  
Acid Detergent Fibre ◽  
Livestock Feeding

The aim of the present study was to determine the nutritional value of Phalaris minor seeds in comparison to conventional cereal grains. P. minor seeds had higher ether extract (6.3%), total ash (7.5%), acid insoluble ash (AIA, 4.5%), acid detergent fibre (ADF, 10.2%), cellulose (5.5%), acid detergent lignin (ADL, 2.0%), neutral detergent insoluble crude protein (NDICP, 4.0%) and acid detergent insoluble crude protein (ADICP, 2.7%) content in comparison to conventional cereal grains. The crude protein of P. minor seeds (12.3%) was similar to that of wheat (12.0%), but higher than other conventional cereal grains including maize (9.0%). The amino acid composition of P. minor seeds was more or less similar to conventional cereal grains. P. minor seeds had a superior mineral profile (Ca, P, Na, K, Mg, Fe, Cu, Zn and Mn) than conventional cereal grains. In vitro evaluation revealed that net gas production was lowest (P less than 0.05) in P. minor seeds (216.37 L/kg DM/24 h). The methane production (L/kg DM/24 h)from P. minor seeds (43.03)was lower (P less than 0.05) than that from wheat (54.33) and barley (57.35). The organic matter digestibility of P. minor seeds (83.27%) was similar to that of barley (81.69%).It was concluded that P. minor seeds could be considered as potential source of nutrients for livestock.

Effects of increasing the inclusion level of Atriplex amnicola in the diet of sheep

2009 ◽  
Vol 49 (11) ◽  
pp. 1029
Author(s):  
M. B. P. Kumara Mahipala ◽  
G. L. Krebs ◽  
P. McCafferty ◽  
K. Dods
Keyword(s):  
Crude Protein ◽  
Energy Content ◽  
Gas Production ◽  
Mineral Contents ◽  
Neutral Detergent Fibre ◽  
Freeze Dried ◽  
Acid Detergent Fibre ◽  
Inclusion Level ◽  
Metabolisable Energy

The effects of increasing the level of inclusion of fresh Atriplex amnicola Paul G.Wilson foliage in an oaten chaff (Avena sativa L.) diet fed to sheep were investigated. Six experimental diets were formulated to contain varying amounts [0, 161, 362, 496, 650 and 836 g/kg diet dry matter (DM)] of A. amnicola, and these diets were fed to individually penned sheep according to a Latin square design. Feed and faecal samples were analysed for crude protein, neutral detergent fibre, acid detergent fibre, acid detergent lignin, and ash contents. Mineral contents and 24 h in vitro gas production were measured in freeze-dried feed samples. As the level of inclusion of A. amnicola increased, digestibility of DM, organic matter and neutral detergent fibre increased (P < 0.05), reaching the peak at the 496 g/kg DM inclusion level and then decreasing (P < 0.05) thereafter. Acid detergent fibre digestibility followed a similar trend, but peak digestibility was at 362 g/kg DM inclusion level. Digestibility of crude protein continued to increase (P < 0.05) with increasing inclusion of A. amnicola. Gas production (24 h) from A. amnicola was lower (P < 0.05) than that from oaten chaff (24.4 v. 45.3 mL/200 mg DM) and also had lower metabolisable energy content (in vitro ME, 6.3 v. 8.6 MJ/kg DM). As the level of inclusion of A. amnicola in the diet increased, gas production and in vitro metabolisable energy content decreased (P < 0.05). The 496 g/kg DM inclusion level provided the optimum N : energy ratio for fermentation of dietary fibre. At this level of inclusion the diet was rich in Na, Ca, P, Mg, K, S, Mn and Zn. High inclusion levels improved the crude protein value of the diet but compromised fibre digestibility.

scholarly journals Assessing the nutritional value of agroindustrial co-products and feed through chemical composition, in vitro digestibility, and gas production technique

2017 ◽  
Vol 39 (3) ◽  
pp. 289 ◽  
Author(s):  
Paula Martins Olivo ◽  
Geraldo Tadeu dos Santos ◽  
Luís Carlos Vinhas Ítavo ◽  
Ranulfo Combuca da Silva Junior ◽  
Eduardo Souza Leal ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Soybean Hulls ◽  
Citrus Pulp

Agroindustrial co-products are a viable alternative for use in animal nutrition. Tests were conducted using eight different types of co-products and feed to evaluate the chemical composition, in vitro digestibility of dry matter, crude protein and neutral detergent fiber, and gas production by them. The co-products tested were: coffee hulls; pelleted citrus pulp; grape residue; soybean hulls; cottonseed; cassava foliage; and foods usually supplied to ruminants: corn silage and ground corn concentrate. Data of in vitro digestibility of dry matter, crude protein and neutral detergent fiber were tested by analysis of variance using the least square method; the results of gas production were interpreted by a non-linear regression by the Gauss-Newton method; and the effects of treatments were evaluated by the Tukey’s test. The coefficients of in vitro digestibility of dry matter, crude protein and neutral detergent fiber of co-products were different. Gas production was also different between co-products and feeds evaluated for the volume of gas produced from the fast and slow degradation fractions, degradation rate, bacterial colonization time, and the total volume of gas produced. The evaluated co-products exhibited greater in vitro dry matter digestibility compared to corn silage, except for cottonseed, grape residue, and cassava foliage. Co-products showed higher values of in vitro crude protein digestibility compared to corn silage, and a reduced in vitro digestibility of neutral detergent fiber, except for pelleted citrus pulp and soybean hulls. Corn silage produced larger volume of gas from the fast degradation fraction compared to the co-products and corn concentrate. Co-products analyzed had appropriate nutritional characteristics according to the techniques applied and can be included in ruminant diets. 

scholarly journals In vitro evaluation of mixed silage of Pennisetum purpureum and orange fleshed sweet potato vines

2019 ◽  
Vol 46 (1) ◽  
pp. 216-224
Author(s):  
R. M. Akinbode ◽  
S. O. Afuape ◽  
K. O. Adebayo ◽  
A. S. Rafiu ◽  
A. O. Adekoya
Keyword(s):  
Sweet Potato ◽  
Methane Production ◽  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Dry Season ◽  
Pennisetum Purpureum ◽  
Lag Phase ◽  
Orange Fleshed Sweet Potato

The study was aimed at determining the in vitro nutritive value of mixed silages of pennisetum purpureum and orange-fleshed sweet potato vines and evaluating their potential of being a new feed resource for ruminants during the dry season. Pennisetum purpureum (Pp) and orange-fleshed sweet potato vines (OFPV) were ensiled in experimental silos bottles after wilting for 24 hours in the following proportions viz:100%Pp (T1), 75%Pp +25%OFPV (T2), 50%Pp +50%OFPV (T3), 25% Pp +75% OFPV (T4) and 100% OFPV (T5). Each treatment was replicated ten (10) times and was kept for 14 and 28 days in the laboratory at a room temperature (20 – 25°C). The chemical composition of the silage at 14 and 28 days was determined. In vitro gas production of the silages was carried out for 48 hours. In vitro fermentation kinetics and gas production parameters of the silages were also estimated. Results revealed that ensiling periods had significant (p<0.05) effect on the dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) contents of the silages. Silage of 28 days had highest CP (13.45%) and lowest NDF and ADF contents (40.10 and 25.48% respectively). Silage containing 100% OFPV recorded highest (p<0.05) crude protein (15.95%), ash (15.81%) and lowest NDF (32.25%) and ADF (22.50%) contents. Gas production was lowest (p<0.05) in T1 at all incubation periods, and it increases with inclusion of OFPV, T5 had significant (p<0.05) highest gas production. Least gas production was observed in T1 at 42 and 48 hours incubation period (15.17 and 17.17 mL/200mg DM respectively). Silage of 28 days had the best potential gas production (37.51 mL). Fractional rate of gas production was higher in 28 days silage (0.06mL/hr), T3 (0.05mL/hr), T4 (0.05mL/hr) and T5 (0.06mL/hr) while lag phase was lowest in these silages. In vitro dry matter digestibility (IVDMD), metabolisable energy (ME) and short chain fatty acids (SCFA) were greater in silage of 28 days (63.73%, 5.01MJ/Kg DM and 0.02mL) with least percentage methane production (33.34%). Also, IVDMD, in vitro organic matter digestibility (OMD), ME, and SCFA increased as level of OFPV in the silages increases with T5 having the highest values for these parameters. Percentage methane production was least (p < 0.05) in T5 (26.55%). It can therefore be concluded that Pennisetum purpureum be supplemented with OFPV up to 75% and ensiled for 28 days or beyond to produce better quality feed resource for ruminants during the dry season. Ensiling 100% OFPV is also highly recommended as it exhibited the best nutritive potential.

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