Probiotic and prebiotic supplementation improving the production performance and immune characteristics of laying hens

2017 ◽  
Author(s):  
Abhed Pandey ◽  
Avtar Singh ◽  
Shanthanagouda Admane Holeyappa ◽  
Harsimranjit Kaur
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Basal Diet ◽  
Production Performance ◽  
Control Group ◽  
Egg Mass ◽  
Feeding Trial ◽  
Treatment Groups ◽  
Cell Mediated Immune Response ◽  
Bacillus Spp

A 16 week feeding trial was conducted with 140 White Leghorn layers of 22-23±0.08 weeks of age which were distributed randomly into seven experimental groups, having 4 replicates with 5 birds in each. The laying hens of control group (T1) were fed a basal diet (CP 18.04%), while in treatment groups T2, T3, T4 basal diet was supplemented with probiotic (containing 5×108 cfu/g of Lactobacillus fermentum, 1×109 cfu/g of Bacillus spp., 1×109cfu/g of Saccharomyces cerevisiae) at levels of 0.5g, 1.0 g and 2.0 g /kg feed, respectively, while in T5, T6, and T7 0.5g, 1.0g and 2.0g/kg feed of prebiotic (mannonoligosacchride of Saccharomyces cell wall-47g, formic acid-32g) was supplemented, respectively. The percent hen day egg production and feed efficiency (kg/dozen eggs and kg/kg egg mass) were improved (P less than 0.05) in probiotic and prebiotic incorporated diets. The mRNA expression levels of Toll like receptors (TLRs) TLR2 and TLR4 was increased (P less than 0.05) when graded levels of the probiotic and prebiotic were supplemented, while, TLR7 did not show any significant change. Thus, it can be inferred that the inclusion of the probiotic and prebiotic could enhance the production performance and immune status of birds by augmenting the T cell mediated immune response.

Egg quality and production performance of laying hens injected with growth hormone and testosterone in the late phase of production

2016 ◽  
Vol 56 (1) ◽  
pp. 147
Author(s):  
H. Mohammadi ◽  
Z. Ansari-Pirsaraei ◽  
S. N. Mousavi ◽  
M. Bouyeh ◽  
A. Gholibeikifard ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Quality Parameters ◽  
Production Performance ◽  
Fixed Dose ◽  
Control Group ◽  
Egg Mass ◽  
Positive Effects

In this study, 160 73-week-old laying hens (HyLine W-36) were used to investigate the changes in production performance and egg quality parameters of aged laying hens after injection of different doses of growth hormone (GH) and a fixed dose of testosterone (Ts). The hens were weighed individually, placed in laying cages and then randomly assigned to four treatments with four replicates of 10 birds each, in a completely randomised design. The experiment was started at the 75th week and lasted until the 82nd week of age. The treatments were: 1: injection of 100 µL distilled water (control group), 2: injection of 500 µg Ts/kg liveweight + 50 µg GH/kg liveweight, 3: injection of 500 µg Ts/kg liveweight + 100 µg GH/kg liveweight and 4: injection of 500 µg Ts/kg liveweight + 150 µg GH/kg liveweight. In this study, ovulation rate (egg production percent), egg mass and feed intake were significantly higher in Treatment 3 than the control group (P < 0.05), and in Treatment 4, feed conversion ratio was significantly higher than for the other experimental groups (P < 0.05). In addition, the birds in Treatment 3 showed greater egg weight, albumen height, eggshell weight, eggshell density and eggshell weight per surface area than the control group (P < 0.05). In Treatment 3, egg length significantly increased compared with Treatments 1 and 4 (P < 0.05), and in Treatments 2 and 4, shell breaking strength was significantly lower than for the control group (P < 0.05). In conclusion, our results showed positive effects of GH and Ts administration on egg quality parameters and production performance in aged laying hens. Taken together, in the present study Treatment 3 was found more effective in improving egg quality and production performance of the old laying hens.

scholarly journals Effect of Date Palm Pollen Supplementation on the Egg Production, Ovarian Follicles Development, Hematological Variables and Hormonal Profile of Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 69
Author(s):  
Mohamed Saleh ◽  
Dariusz Kokoszyński ◽  
Mohamed Abd-Allah Mousa ◽  
Ahmed Abdel-Kareem Abuoghaba
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Ovarian Follicle ◽  
Basal Diet ◽  
Ovarian Follicles ◽  
Control Group ◽  
Egg Mass ◽  
Egg Weight ◽  
Hormonal Profile ◽  
Ovarian Follicle Development

This experiment studied the effect of DPP supplementation in the laying hens’ diet on the ovarian follicle development, hematological variables and hormonal profile of laying hens. Eighty-four, 78-week-old, Lohman LSL hybrids layers were equally divided into four groups (4 groups × 7 replicates × 3 hens); hens in the 1st group were fed a basal diet (control), while those in the 2nd, 3rd and 4th groups, were fed on the same diet and supplemented with 1.25, 2.5 and 5.0 g DPP/kg diet. The results showed that the egg weight, egg mass and laying rate of laying hens treated with DPP levels were significantly increased compared to those of the hens in the control group. Egg weight, egg surface area, albumen quality and percentage of the yolk in treated hens significantly increased compared with controls. The increased DPP levels in laying hens‘ diet significantly (p < 0.05) increased WBC, Hb and TAC, while heterophil/lymphocyte (H/L ratio) significantly decreased. Additionally, the concentrations of FSH and LH and the weights of ovary and oviduct in the treated hens significantly (p < 0.05) increased compared with the control. In conclusion, the DPP supplementation in the hen diet significantly improved egg production, EW, H/L ratio, ovarian follicles, FSH and LH hormones concentrations.

scholarly journals Effect of Lactobacillus plantarum supplementation on production performance and fecal microbial composition in laying hens

2019 ◽  
Vol 14 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Hongxing Qiao ◽  
Hongtao Shi ◽  
Liheng Zhang ◽  
Yuzhen Song ◽  
Xiaojing Zhang ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Egg Production ◽  
Laying Hens ◽  
Test Group ◽  
Basal Diet ◽  
Fecal Microbiota ◽  
Production Performance ◽  
Control Group ◽  
Microbiota Composition ◽  
Microbial Composition

AbstractThe present study was performed to investigate the effects of dietary supplementation with Lactobacillus plantarum (CGMCC1.557) on egg production and fecal microbiota composition in laying hens. Sixty Hy-Line Brown laying hens (18 weeks old) were randomly divided into two groups. The control group was fed a basal diet only, and the test group was fed basal diet supplemented with a final concentration of 1.0 × 109 CFU/mL during the 10-week experimental period. Egg production and fecal microbiota composition were both assessed in 28-week-old hens using high-throughput sequencing technology. The results showed that, compared with the control group, the test group exhibited increased laying and feed intake rates (p < 0.05). At the genus level, Lactobacillus was more abundant in the test group compared with the control group (p < 0.05). Conversely, Romboutsia was more abundant in the control group compared with the test group (p < 0.05). This study provides us with an insight into the potential use of L. plantarum as a food supplement in the laying hen industry. the study also provides us with a better understanding of the interplay between L. plantarum and the fecal microbiota of laying hens.

scholarly journals Effects of Dietary Inclusion of Dry Hydrastis canadensis on Laying Performance, Egg Quality, Serum Biochemical Parameters and Cecal Microbiota in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1381
Author(s):  
Tzuen-Rong J Tzeng ◽  
Tzu-Yu Liu ◽  
Chiao-Wei Lin ◽  
Pei-En Chang ◽  
Pei-Xin Liao ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Egg Production ◽  
Laying Hens ◽  
Basal Diet ◽  
Response To Treatment ◽  
Control Group ◽  
Hydrastis Canadensis ◽  
Laying Performance ◽  
Cecal Microbiota ◽  
Dietary Treatments

Alternative growth promoters are able to not only effectively replace the traditional use of antibiotics but also provide additional health benefits for livestock and reduce food safety concerns. This study investigated the effects of dry Hydrastis canadensis on the laying performance and fecal microbial community of laying hens. Twenty-four Lohmann (LSL, white layer strain) hens were reared from 40 to 48 weeks of age and randomly allotted to four dietary treatments (six birds/treatment). The dietary treatments comprised a basal diet with no treatment as control, a basal diet plus 0.6% powder of dry Hydrastis canadensis roots (R) or leaves (L), and a basal diet plus 0.6% powder of a mixture of dry Hydrastis canadensis roots and leaves (1:1, LR). No mortality was observed in the whole experimental period. The results indicated that albumen height in the LR group was significantly greater than that in the control group. The diet supplemented with Hydrastis canadensis had no significant effects on egg production rate, egg weight, eggshell strength, eggshell thickness, Haugh unit, or yolk height during the whole experimental phase. However, principal coordinate analysis, comparative heat map analysis, and cluster dendrogram analysis of cecal microbiota showed distinct clusters among the groups treated with Hydrastis canadensis and the control group. Regarding blood biochemical parameters, serum cholesterol levels were significantly lower in all Hydrastis canadensis-treated groups compared with those in the control group. Moreover, serum low-density lipoprotein levels were lower in hens supplemented with the leaf of Hydrastis canadensis. The abundances of the phyla Fusobacteria and Kiritimatiellaeota were increased (p < 0.05) in laying hens fed with 0.6% Hydrastis canadensis leaves, whereas the abundance of the phylum Firmicutes in cecum digesta decreased in response to treatment with Hydrastis canadensis roots and leaves. The relative abundance of the Fusobacterium genus was higher in the LR group compared with that in the control. On the contrary, we found a different trend in the Synergistes genus. The potential influences of these microbiota on the performance of laying hens were discussed. The results demonstrate that Hydrastis canadensis can improve the egg albumen height and modulate the cecum digesta microbiota composition of laying hens.

Transcriptomics and Transmission Ultrastructural Examination Reveals the Nephrotoxicity of Cadmium in Laying Hens

2021 ◽  
Author(s):  
Man Zhao ◽  
Wenbo He ◽  
Can Tao ◽  
Beiyu Zhang ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Laying Hens ◽  
Basal Diet ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Renal Tubules ◽  
Rna Seq ◽  
Ultrastructural Examination ◽  
Treatment Groups ◽  
Kegg Pathway Analysis ◽  
Transcriptome Expression

Abstract The objective of this study was to reveal the effects of cadmium (Cd) on ultrastructural changes, oxidative stress, and transcriptome expression in the kidneys of laying hens. Seventy-two healthy Hy-Line brown laying hens at 41 weeks old were randomly allocated to four treatment groups with six replicates. The control group received a basal diet without additional Cd incorporation, and the other three treatment groups received diets supplemented with 15, 30, or 60 mg Cd /kg of feed. After 6 weeks of exposure the results show that administration of 60 mg/kg Cd significantly reduced (P < 0.05) eggshell thickness. With an increase in the Cd concentration in feed, the concentrations of renal Zn, Fe also had changed. Renal histopathology and ultrastructure also showed aggravated damage to glomeruli and renal tubules, and the deformation of nuclei and mitochondria in all Cd treatment groups. With an increase in Cd in feed, the activity of GPX and CAT was significantly reduced (P＜0.05), while the activity of T-AOC was decreased (P＜0.05) only in the 60 mg/kg Cd group. RNA-seq analysis revealed that 410 genes displayed differential expression (≥ 1.5-fold) in the 60 mg/kg supplementation group, compared to the control group. GO and KEGG pathway analysis results showed that Cd affected many genes involved in mitochondria and ion transport. In conclusion, this study elaborates the mechanisms underlying renal toxicity caused by Cd, which might provide target candidate genes for alleviating Cd poisoning in laying hens.

scholarly journals Production performance and egg quality of White Rock laying hens fed diets containing whole linseed

2020 ◽  
Vol 20 (1-2) ◽  
pp. 48-53
Author(s):  
H Khatun ◽  
MN Islam ◽  
MA Rashid ◽  
MY Ali ◽  
MSK Sarker
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Control Diet ◽  
Production Performance ◽  
Egg Mass ◽  
Feed Conversion ◽  
Egg Weight ◽  
Quality Aspects ◽  
Significant Difference

This study was conducted to know the production performance of laying hen by using different graded level of linseed. At 56 weeks of age, a total of 160 laying hens were raised up to 63 weeks of age. All hens were fed a basal corn soyabean meal diet. The experiment was allowed 4 dietary treatments (T0, no addition of whole linseed in the basal diet; T1,  addition of 5% whole linseed; T2, addition of 10% whole linseed and T3, addition of 15% whole linseed) with 5 replications and per replication contains eight hens. Egg production, feed intake, egg weight of each treatment was recorded. Egg samples were collected and analyzed for egg quality. The results showed that egg production and egg mass (kg) exhibited for all dietary treatment groups were unaffected (P>0.05) by feeding linseed to the laying hens. Feed Conversion Ratio (FCR) was found significant difference within the four dietary group (P<0.05). Egg quality aspects such as yolk weight, egg weight, HU and yolk index were not differed significantly (P>0.05) among treatments.  A trend for lower yolk weight was observed in birds fed 10% linseed, where as the highest yolk weight percentage was recorded in control diet. There was no statistical difference among the different diets for yolk index. The results of the present trial suggested that feeding linseed to the laying hens has no detrimental effects on egg production and egg quality. Bangladesh J. of Livestock Res. 20(1-2): 48-53, Jan-Dec 2013

scholarly journals Effect of Phytase Supplementation on the Performance of Babcock-380 Layer Hens

2021 ◽  
Vol 1 (1) ◽  
pp. 36-41
Author(s):  
MD. Ruhul Amin ◽  
Elliyana Nadia Hamidi
Keyword(s):  
Body Weight ◽  
Egg Production ◽  
Egg Size ◽  
Basal Diet ◽  
Experimental Period ◽  
Egg Mass ◽  
Egg Weight ◽  
Treatment Groups ◽  
Microbial Phytase ◽  
Production Group

 In order to examine the effect of phytase supplementation in the diet of laying hen this experiment was conducted with 30 commercial hybrid Babcock-380 layers of 73 weeks of age. The hens were grouped into 3 treatment groups and given microbial phytase (Rena Phytase 400) at levels 2.5, 1.5 and 0 (control) g/kg feed in addition to their basal diet (110 g commercial pellet/day). Records on egg production/group/day, egg mass weight/day, body weight of hens at the end of experimental period were taken and analysed. Results revealed that hen house egg production (HHEP) and egg weight (EW) varied (p<0.05) among the treatment groups but level of phytase did not affect (p<0.05) body weight of hens. Both HHEP and EW were in order of 2.5>1.5>0 g/kg feed phytase group and all the means were significantly (p<0.05) different from each other within treatment groups. Microbial phytase supplementation in layer ration proved to be beneficial in terms of egg production and egg size even after 72 weeks of age of hens. Layer farms in Malaysia can use this technology to earn more profit.

scholarly journals The Effect of Clove Oil Supplementation in Laying Hen Diets on Performance, Egg Quality, Some Blood Parameters, and Yolk TBARS

2021 ◽  
Vol 9 (12) ◽  
pp. 2213-2218
Author(s):  
Melek Şehitoğlu ◽  
Hatice Kaya
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Live Weight ◽  
Blood Parameters ◽  
Feed Consumption ◽  
Control Group ◽  
Clove Oil ◽  
Feed Conversion ◽  
Treatment Groups

In this study, it was investigated the effect of clove oil supplementation at increasing levels into laying hens’ diets on performance, egg quality traits, some blood parameters and yolk TBARS (Thiobarbituric Acid Reagent) values. For this purpose 96 Lohman white laying hens, 28 weeks of age, were divided into four treatment groups. Control group was fed with basal diet (C) and treatment groups were fed with diets formed by addition of clove oil at 50 ppm (CO1), 100 ppm (CO2) and 150 ppm (CO3), respectively. During the trial, feed and water were given as ad-libitum, and poultry house was illuminated for 17 hours. Experiment lasted for 13 weeks. Addition clove oil at increasing rates into diet did not affect the live weight. The data analysed as polynomial showed that supplementation of clove oil into layer diet linearly improved feed conversion ratio and increased the egg production. But, daily feed consumption, egg weight, damaged egg ratio and egg quality parameters were not affected by treatments. Serum parameters such as triglyceride, glucose, aspartate aminotransferase, alanine aminotransferase and calcium were not affected by the clove oil supplementation. TBARS values in C, CO1, and CO2 were found higher than the CO3 group fed with diet including 150 ppm clove oil. In conclusion, clove oil at 150 ppm level in diets of laying hens could be used due to extend the egg shelf life and to decline serum cholesterol content.

scholarly journals Effects of feed supplementation with manganese from its different sources on performance and egg parameters of laying hens

2014 ◽  
Vol 59 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
K. Venglovská ◽  
Ľ. Grešáková ◽  
I. Plachá ◽  
M. Ryzner ◽  
K. Čobanová
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Protein Hydrolysate ◽  
Basal Diet ◽  
Egg Yolk ◽  
Control Group ◽  
Feed Supplementation ◽  
Positive Effects ◽  
Dietary Treatments

The objective of this study was to compare the effects of feed supplementation of laying hens with manganese from its inorganic and organic sources on performance and some parameters of egg quality. Ninety-six hens at 20 weeks of age were randomly allocated to 4 dietary treatments, each consisting of 6 replicates (4 birds per replicate). The control group was fed unsupplemented basal diet (BD) with only natural background Mn level of 46.4 mg/kg feed. For the three experimental treatments, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Pro) or Mn-chelate of glycine hydrate (Mn-Gly). After 8 weeks of dietary treatments the egg production, egg weight, feed intake, and feed efficiency were not affected by dietary treatments. Regardless of the sources, Mn supplementation to feed resulted in significantly decreased percentages of cracked eggs compared to the unsupplemented control group. The thickness, weight, proportion, and index of eggshell were significantly elevated in all groups supplemented with Mn. The intake of Mn-Gly resulted in considerably increased Mn deposition in egg yolk compared to the control eggs. In the control and Mn-sulphate groups yolk malondialdehyde (MDA) started to increase after 20 and 30 days of egg storage respectively, whereas in eggs from hens given organic Mn-sources this parameter was not affected up to 40 days. Although there were no significant differences in MDA values between the treatments until 20 days of storage, the Mn-sulphate group showed significantly higher MDA concentration in yolks compared to the control group after 30 days of storage. These results demonstrate that supplementation of hens&rsquo; diet with Mn has positive effects on eggshell quality. Feed supplementation with Mn from organic sources appears to be more effective in preventing yolk lipid oxidation during cold storage of eggs than that from Mn-sulphate. &nbsp;

Supplementation of organic minerals and its effect on production performance and egg quality of laying hens

2020 ◽  
pp. 12-16
Keyword(s):  
Feed Intake ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Production Performance ◽  
Control Group ◽  
Adaptation Period ◽  
Egg Shell ◽  
Organic Minerals

The present study was conducted to find the effect of different levels of organic minerals on egg production and egg quality of laying hens. A total of 45 laying hens were selected and divided into three groups with 3 replicates of 5 birds each. The control group was provided with commercial ration having inorganic minerals and the other two groups were supplemented with organic minerals at the rate of 7.5 (OM-50) and 15 mg/kg (OM-100). The experiment lasted for 5 weeks including 1 week of adaptation period. Overall feed intake was significantly higher (P<0.05) in the control. Significantly lower feed intake was recorded in group OM-100. Numerically lower (better) FCR was recorded in OM-100 at all recorded stages. Significantly (P<0.05) higher egg weight, egg shell weight and egg shell thickness was found in OM-100 compared to the control. Similarly, albumin height, Haugh unit and yolk weight were significantly (P<0.05) higher in M-100. It was concluded from the present results that organic minerals at the level of 50 and 100 mg/kg improved the performance and egg quality of laying hens. However, better results were found in OM-100.

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