scholarly journals Effects of Dietary Inclusion of Dry Hydrastis canadensis on Laying Performance, Egg Quality, Serum Biochemical Parameters and Cecal Microbiota in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1381
Author(s):  
Tzuen-Rong J Tzeng ◽  
Tzu-Yu Liu ◽  
Chiao-Wei Lin ◽  
Pei-En Chang ◽  
Pei-Xin Liao ◽  
...  

Alternative growth promoters are able to not only effectively replace the traditional use of antibiotics but also provide additional health benefits for livestock and reduce food safety concerns. This study investigated the effects of dry Hydrastis canadensis on the laying performance and fecal microbial community of laying hens. Twenty-four Lohmann (LSL, white layer strain) hens were reared from 40 to 48 weeks of age and randomly allotted to four dietary treatments (six birds/treatment). The dietary treatments comprised a basal diet with no treatment as control, a basal diet plus 0.6% powder of dry Hydrastis canadensis roots (R) or leaves (L), and a basal diet plus 0.6% powder of a mixture of dry Hydrastis canadensis roots and leaves (1:1, LR). No mortality was observed in the whole experimental period. The results indicated that albumen height in the LR group was significantly greater than that in the control group. The diet supplemented with Hydrastis canadensis had no significant effects on egg production rate, egg weight, eggshell strength, eggshell thickness, Haugh unit, or yolk height during the whole experimental phase. However, principal coordinate analysis, comparative heat map analysis, and cluster dendrogram analysis of cecal microbiota showed distinct clusters among the groups treated with Hydrastis canadensis and the control group. Regarding blood biochemical parameters, serum cholesterol levels were significantly lower in all Hydrastis canadensis-treated groups compared with those in the control group. Moreover, serum low-density lipoprotein levels were lower in hens supplemented with the leaf of Hydrastis canadensis. The abundances of the phyla Fusobacteria and Kiritimatiellaeota were increased (p < 0.05) in laying hens fed with 0.6% Hydrastis canadensis leaves, whereas the abundance of the phylum Firmicutes in cecum digesta decreased in response to treatment with Hydrastis canadensis roots and leaves. The relative abundance of the Fusobacterium genus was higher in the LR group compared with that in the control. On the contrary, we found a different trend in the Synergistes genus. The potential influences of these microbiota on the performance of laying hens were discussed. The results demonstrate that Hydrastis canadensis can improve the egg albumen height and modulate the cecum digesta microbiota composition of laying hens.

2014 ◽  
Vol 59 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
K. Venglovská ◽  
Ľ. Grešáková ◽  
I. Plachá ◽  
M. Ryzner ◽  
K. Čobanová

The objective of this study was to compare the effects of feed supplementation of laying hens with manganese from its inorganic and organic sources on performance and some parameters of egg quality. Ninety-six hens at 20 weeks of age were randomly allocated to 4 dietary treatments, each consisting of 6 replicates (4 birds per replicate). The control group was fed unsupplemented basal diet (BD) with only natural background Mn level of 46.4 mg/kg feed. For the three experimental treatments, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Pro) or Mn-chelate of glycine hydrate (Mn-Gly). After 8 weeks of dietary treatments the egg production, egg weight, feed intake, and feed efficiency were not affected by dietary treatments. Regardless of the sources, Mn supplementation to feed resulted in significantly decreased percentages of cracked eggs compared to the unsupplemented control group. The thickness, weight, proportion, and index of eggshell were significantly elevated in all groups supplemented with Mn. The intake of Mn-Gly resulted in considerably increased Mn deposition in egg yolk compared to the control eggs. In the control and Mn-sulphate groups yolk malondialdehyde (MDA) started to increase after 20 and 30 days of egg storage respectively, whereas in eggs from hens given organic Mn-sources this parameter was not affected up to 40 days. Although there were no significant differences in MDA values between the treatments until 20 days of storage, the Mn-sulphate group showed significantly higher MDA concentration in yolks compared to the control group after 30 days of storage. These results demonstrate that supplementation of hens&rsquo; diet with Mn has positive effects on eggshell quality. Feed supplementation with Mn from organic sources appears to be more effective in preventing yolk lipid oxidation during cold storage of eggs than that from Mn-sulphate. &nbsp;


Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2420
Author(s):  
Ewa Tomaszewska ◽  
Sylwester Świątkiewicz ◽  
Anna Arczewska-Włosek ◽  
Dorota Wojtysiak ◽  
Piotr Dobrowolski ◽  
...  

The aim of the experiment was to assess the effect of dietary alpha-ketoglutarate (AKG) supplementation on performance, serum hormonal indices, duodenum and jejunum histomorphometry, meat quality characteristics, bone quality traits and cartilage degradation in laying hens with a mature skeletal system. Forty-eight 30 week-old Bovans Brown laying hens were randomly assigned to a control group or the group fed the basal diet plus 1.0% AKG. The experimental trial lasted 30 weeks. The supplementation of AKG increases blood serum content of leptin, ghrelin, bone alkaline phosphatate and receptor activator of nuclear factor kappa-Β ligand, while osteoprotegerin and osteocalcin decrease. While dietary AKG was given to laying hens negatively influenced villus length, crypt depth, villus/crypt ratio and absorptive surface area in duodenum and jejunum, these changes have no effect on feed intake, weight gain, nor laying performance. In breast muscles, no significant changes in skeletal muscle fatty acid composition were observed, however, a higher shear force and decreased cholesterol content following AKG supplementation were noted, showing the improvement of muscle quality. While dietary AKG supplementation did not affect the general geometric and mechanical properties of the tibia, it increased collagen synthesis and enhanced immature collagen content. In medullary bone, an increase of bone volume fraction, trabecular thickness, fractal dimension and decrease of trabecular space were observed in AKG supplemented group. The trabeculae in bone metaphysis were also significantly thicker after AKG supplementation. AKG promoted fibrillogenesis in articular cartilage, as indicated by increased cartilage oligomeric matrix protein immunoexpression. By improving the structure and maintaining the proper bone turnover rate of highly reactive and metabolically active medullar and trabecular bones AKG showed its anti-osteoporotic action in laying hens.


2020 ◽  
Vol 100 (3) ◽  
pp. 411-417
Author(s):  
Huan Shi ◽  
Wan Lin Zhang ◽  
In Ho Kim

An experiment was carried out to investigate the effect of Bacillus subtilis RX7 and B2A supplementation on egg production, egg quality, blood profile, and excreta Salmonella population in laying hens. A total of 192 Hy-Line Brown laying hens (40-wk-old) were randomly assigned into four dietary treatments, each of which was replicated eight times with six hens per replicate in a 5 wk trial. The dietary treatments were NC, which is a basal diet without antibiotics or Bacillus subtilis; PC, which is an NC + 0.5 g kg−1 antibiotic (virginiamycin) diet; BSR, which is an NC + 0.5 g kg−1 Bacillus subtilis RX7 (1.0 × 109 colony forming units g−1); and BSB, which is an NC + 0.5 g kg−1 Bacillus subtilis B2A (1.0 × 109 colony forming units g−1). The results showed that hens receiving BSB diets had increased (P < 0.05) eggshell thickness over hens fed the NC, PC, and BSR diets (week 3). The excreta Salmonella counts in the PC, BSR, and BSB groups were lower than the NC group (P < 0.05). These results indicate that dietary inclusion of Bacillus subtilis RX7 and B2A strains effectively decreased excreta Salmonella counts without any detrimental effects on the performance of laying hens.


2021 ◽  
Vol 43 (1) ◽  
pp. 224-232
Author(s):  
O. M. Agunbiade ◽  
P. A. Onimisi ◽  
J. J. Omage

Sorghum is the primary alternative feedstuff for corn in some developed countries in the production of poultry. Thus, four hundred and fifty 32 weeks old Lohmann Brown laying hens were used in a study to evaluate the effect of replacing maize with sorghum with or without enzymes supplementation, on egg laying performance and egg quality characteristics of laying hens. Birds were distributed randomly into six dietary treatments with three replicates per treatment, each replicate had 25 birds with uniform initial group weights. The treatments included maize without enzymes (T1); sorghum without enzymes (T2); as controls respectively, while T3, T4, T5, and T6 contained sorghum based diets with phytase, protease, G2G, and a combination of protease and G2G respectively. Laying performance, cost of production, and egg quality characteristics were determined for the period of the trial, 33-56 weeks of age of the birds. All data obtained were subjected to analysis of variance, using the completely randomized design (CRD) and significant differences among treatment means were compared using the Tukey test. Significant (p<0.05) differences were observed among dietary treatments but with no defined trend observed for egg production traits. Daily feed intake was significantly (p<0.05) higher in maize control diet compared to the other treatments. Diets supplemented with phytase (T3), protease (14), and combination of protease and roxazyme G2G (T6) had significantly (p<0.05) better feed conversion ratio with T3 having the lowest value of 4.01 compared to the other treatments. For feed cost/dozen egg (N), Kg feed/dozen eggs, income above feed cost at N30 per egg, and income above control treatment; phytase (T3), protease (T4), roxazyme 626 (T5), and protease + roxacyme G2G (T6) diets in this study showed better performance than the control. Sorghum diet without enzyme had significantly (p<0.05) least performance for egg number. Hen housed egg production (HHP) and hen day egg production (HDP) compared to the other treatments while sorghum diet with phytase had the highest value (60.12g/day) for average egg weight compared to the other treatments. Egg quality characteristics showed the best performance for dietary treatments supplemented with phytase (T3) in all the other parameters considered, apart from the albumen height, yolk diameter, and yolk index. It was concluded therefore, that sorghum with phytase and protease supplementations can be used in layers' diets as a substitute for maize for optimum egg production.


Author(s):  
Yang Jiao ◽  
Rajesh Jha ◽  
Wan Lin Zhang ◽  
In Ho Kim

The present study evaluated the effects of dietary supplementation of chitooligosaccharide (COS) on egg production, egg quality and blood profiles in laying hens. A total of 192 Hy-Line Brown laying hens (initial age- 46 weeks) were allocated to one of four treatments with eight replications per treatment and six adjacent cages as replication for eight-weeks study. Dietary treatments consisted of CON, basal diet; COS1, CON + 0.1% COS; COS2, CON + 0.2% COS; and COS3, CON + 0.3% COS. Linear improvement (p Lass Than 0.05) was observed in egg quality and high-density lipoprotein cholesterol (HDL/C) concentration at subsequent weeks. In week 8, linear (P=0.04) improvement was observed in haugh unit of egg with the increase in the dose of COS. In addition, linear effects (p Lass Than 0.05) on eggshell thickness at week 3, 5, 6, 7 and 8 were detected as the level for increased COS. In conclusion, the supplementation of COS in laying hens diets linearly improved the egg quality and the HDL/C concentration. Thus, COS has the potential to be used as a substitute to antibiotics in layer hen’s diets.


2017 ◽  
Vol 2 (4) ◽  
pp. 547-554 ◽  
Author(s):  
Mst Nasrin Banu ◽  
Md Bazlar Rashid ◽  
Md Mahmudul Hasan ◽  
Fahima Binte Aziz ◽  
Md Rakibul Islam ◽  
...  

The study was conducted to determine the effect of antiprolactin drug (Bromergon®) and Peppermint (Mentha piperita L.) on broodiness, laying performance and egg quality in indigenous hens. The effect of modulation of prolactin concentration on egg production, sequence length and inter sequence pauses were studied by analyzing the oviposition records. Total sixty indigenous laying hens (30-40 weeks of age), were assigned for treatment with peppermint solution and Bromergon®. Sixty laying hens were randomly divided into 4 groups (A, B, C and D) and each group remained 15 hens. Group A was kept for control, Group B was treated with bromocriptine (Bromergon® SANDOZ) orally @ 640 ?g per bird per day, Group C with 50% peppermint solution @ 10 g per bird orally and Group D was with peppermint and Bromergon® combined at previous dose. Over the course of trial, incremental dietary peppermint (Group C) significantly (p?0.05) increased egg production, body weight of treated indigenous hens than other groups. Egg shell percentage, thickness and haugh unit of hens fed diets supplemented with peppermint were greater than that of hens fed the control diet. However, peppermint supplementation did not influence other egg quality characteristics like albumen and yolk percentages and albumen height. The treated birds had comparatively longer sequences and fewer pauses. It is concluded that the physiological pauses occur during ovulatory sequences can be disrupted effectively using Bromergon® and peppermint. Prolactin levels modulated which may interfere with follicular recruitment and subsequent oviposition thereby improves egg laying potential of the indigenous hens.Asian J. Med. Biol. Res. December 2016, 2(4): 547-554


Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 69
Author(s):  
Mohamed Saleh ◽  
Dariusz Kokoszyński ◽  
Mohamed Abd-Allah Mousa ◽  
Ahmed Abdel-Kareem Abuoghaba

This experiment studied the effect of DPP supplementation in the laying hens’ diet on the ovarian follicle development, hematological variables and hormonal profile of laying hens. Eighty-four, 78-week-old, Lohman LSL hybrids layers were equally divided into four groups (4 groups × 7 replicates × 3 hens); hens in the 1st group were fed a basal diet (control), while those in the 2nd, 3rd and 4th groups, were fed on the same diet and supplemented with 1.25, 2.5 and 5.0 g DPP/kg diet. The results showed that the egg weight, egg mass and laying rate of laying hens treated with DPP levels were significantly increased compared to those of the hens in the control group. Egg weight, egg surface area, albumen quality and percentage of the yolk in treated hens significantly increased compared with controls. The increased DPP levels in laying hens‘ diet significantly (p < 0.05) increased WBC, Hb and TAC, while heterophil/lymphocyte (H/L ratio) significantly decreased. Additionally, the concentrations of FSH and LH and the weights of ovary and oviduct in the treated hens significantly (p < 0.05) increased compared with the control. In conclusion, the DPP supplementation in the hen diet significantly improved egg production, EW, H/L ratio, ovarian follicles, FSH and LH hormones concentrations.


Author(s):  
Da-Hye Kim ◽  
Yoo-Kyung Lee ◽  
Sang-Ho Kim ◽  
Kyung-Woo Lee

The present study investigated the effect of different ambient temperature and relative humidity (RH) but equal temperature-humidity index (THI) on laying performance, egg quality, heterophil to lymphocyte ratio (H/L ratio), corticosterone (CORT) concentration in blood, yolk and albumen, and plasma biochemical parameters in laying hens. One hundred and twenty commercial hens (Hy-Line Brown) aged 60 weeks were allocated into 2 environmental chambers. Laying hens were subjected to either one of two thermal treatments, i.e., 26&ordm;C and 70% RH (LH75) and 30&ordm;C and 30% RH (HL75) for 28 days. Both thermal treatments had equal THI being 75. Neither LH75 nor HL75 affected (P &gt; 0.05) laying performance including egg production, egg weight, egg mass, feed intake, and feed conversion ratio. Plasma biochemical parameters such as total cholesterol, high-density lipoprotein cholesterol, triglyceride, calcium, magnesium, and phosphorus was not altered (P &gt; 0.05) by thermal treatments. As to the stress indicators, both environment regimes failed (P &gt; 0.05) to affect blood H/L ratio and CORT levels in plasma, yolk and albumen although albumen CORT levels were elevated (P &lt; 0.05) in LH75 vs. HL75 at days 3, 7, and 28. In conclusion, our study suggests that laying hens performed and responded equally when they were exposed to equal THI environment conditioned from either 26&ordm;C and 70% RH or 30&ordm;C and 30% RH. The results of this study will be served as a scientific basis for management decisions and handling under thermally challenging conditions.


2021 ◽  
Vol 8 ◽  
Author(s):  
Kai Qiu ◽  
Uchechukwu Edna Obianwuna ◽  
Jing Wang ◽  
Hai-jun Zhang ◽  
Guang-hai Qi ◽  
...  

In the context of increasing awareness on the dietary supplementation of organic selenium in commercial poultry production and ensuring safe egg production, the present study investigated the effects of selenium on the pharmacokinetics of the therapeutic use of florfenicol and enrofloxacin from perspectives of laying performance, selenium deposition in eggs, and drug residue in plasma, organs, and eggs. A 2 × 3 factorial arrangement with two kinds of drugs (florfenicol vs. enrofloxacin, 200 mg/kg) and three levels of dietary organic selenium SCIP (selenium conjugated to insect protein) (0, 2, and 5 mg/kg) was designed together with a blank control group. Healthy Hy-Line Brown laying hens (n = 252, 40-week-old and 90.0 ± 1.7% of egg production rate) were randomly allocated into one of seven treatments with six replicates and six hens per replicate. The experiment lasted for 42 days and consisted of three periods (adjusted stage, depositional stage, and eliminating stage) of 14 days each. These stages entail feeding of the laying hens with basal diets, addition of drugs and selenium synchronously into the diets, drug withdrawal from diet, and supply of selenium uninterruptedly in the diet. Egg production and feed intake were recorded on daily and weekly bases, respectively. The selenium content in egg yolk, egg white, and whole eggs and the drug residues in eggs, plasma, liver, kidney, and breast muscle were determined on days 2, 3, 5, 6, 7, 9, 11, and 14 of the depositional and eliminating stages. There was no significant difference (p &gt; 0.05) in egg production among the dietary treatments, but feed intake decreased significantly (p &lt; 0.05) in the drug treatment group compared to other groups. Dietary organic selenium decreased the residue of drugs in tissues and eggs, while the metabolism and deposition of selenium in laying hens were suppressed due to drug effects. The results of the present study are of significance to enrich the knowledge of the pharmacokinetics of florfenicol and enrofloxacin in laying hens and ensure the quality of poultry products.


2021 ◽  
Vol 6 (3) ◽  
pp. 105-112
Author(s):  
C. A. Essien ◽  

Twelve week feeding trial was conducted to investigate the effect of 10 and 20% replacement of maize with Icacinia manni meal processed in saline on the laying performance, egg quality characteristics, internal organ evaluation, hematological and serum biochemistry of laying hens. Three experimental diets were formulated in which Icacinia manni processed in saline replaced maize at 0% for T1 (control) 10 and 20% for T2 and T3, respectively. One hundred and eighty laying hens (Isa Brown) with 6 weeks of laying life were randomly allotted to three dietary treatments with nine replicates of twenty birds per replicate in a completely randomized design. The laying performance, carcass/organs, hematology and serum biochemistry, egg quality analysis showed no significant differences (p>0.05) at the end of the experiment. The values for hen-day production of birds fed 10% Icacinia manni processed in saline showed numerical increase for hen day egg production and egg weight over the control and 20% groups (T1 and T3 respectively). The study revealed that Icacinia manni processed in saline can replace 20% maize in layers diets without detrimental effect on the performance, egg quality characteristics, internal organs, haematology and serum biochemical indices.


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