Immunomodulatory Efficiency of Abelmoschus esculentus in Swiss Albino Mice

Background: Immunomodulation through natural or synthetic substances may be considered as an alternative for prevention and cure of infections. Methods: A total of nineteen groups of Swiss albino mice were experimented for immunological studies with an inclusion of control and immunised control. Lymphocyte count and DTH response in the experimental groups after the administration of plant drugs. The result showed remarkable changes in all kind of treated animals when compared to control. The increment in ‘B’ and “T lymphocyte number was much pronounced in mice by the administration of A. esculentus in combination with immuno-enhansive drug. Result: In DTH responses directly correlated with cell mediated immunity and were found to be highest at the maximum dose (100 mg/Kg) of plant extracts A. esculuntus. From the results it was clear that the plant extract induced immunomodulating potential of the test animal. On administration of plant extract an enhanced and visible DTH responses were observed.

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HAEMATO-BIOCHEMICAL AND IMMUNOLOGICAL CHANGES IN EXPERIMENTALLY INDUCED ACETAMIPRID TOXICITY IN SWISS ALBINO MICE

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/tijovsab.v12i1.11235 ◽

2016 ◽

Vol 12 (1) ◽

Author(s):

D.T. Fefar ◽

Ankita N. Brahmbhatt ◽

B.P. Joshi ◽

D.J. Ghodasara

Keyword(s):

Dose Level ◽

Significant Rise ◽

High Dose ◽

Cell Mediated Immunity ◽

Swiss Albino Mice ◽

Mean Values ◽

Group Iii ◽

Treatment Groups ◽

Immunological Effects ◽

Albino Mice

A study was conducted on 5 weeks old 64 (32 male and 32 female) Swiss albino mice to assess the haemato-biochemical and immunological effects of acetamiprid. All the male and female mice were randomly divided into eight different groups. The groups I (male) and II (female) served as controls whereas remaining groups served as treatment groups and were administered acetamiprid at the daily dose rate of 20, 10, 5 mg/kg body weight in males(Group III, V, VII) and females (Group IV, VI,VIII),respectively for 28 days. After 28 days treatment, blood samples were collected for hematological, biochemical as well as immunological analysis. There was significant decrease in haematological parameters like Hb, TEC, TLC, neutrophils and lymphocytes count in high dose groups and revealed potential adversity of acetamiprid at rates of 20 mg/kg/day on haematopoetic system of mice. A dose dependent significant rise in mean values of AST and ALT was observed in treatment groups, whereas there was significant decrease in total protein and albumin and increase in BUN in high and mid dose treated groups, irrespective of sex of mice. Dinitroflurobenzene (DNFB) test conducted to assess the cell mediated immunity revealed the toxic effect of acetamiprid on cell mediated immunity of mice at dose level of 10 mg/kg/day. The mice of high dose group revealed a significant decrease in HA titer and indicated the immunotoxic potential of acetamiprid at dose level of 20 mg/kg/day.

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EFFECT OF OPUNTIA ELATIOR ON CHOLESTEROL LEVEL ALTERATIONS INDUCED BY GAMMA RADIATION IN SWISS ALBINO MICE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i3.31294 ◽

2019 ◽

pp. 578-581

Author(s):

KAVITA POONIAM ◽

JAIMALA SHARMA

Keyword(s):

Side Effects ◽

Gamma Radiation ◽

Cholesterol Level ◽

Plant Extract ◽

Treated Group ◽

Blood Cholesterol ◽

Blood Cholesterol Level ◽

Swiss Albino Mice ◽

Radiation Induced ◽

Albino Mice

Objectives: Radiotherapy is deleterious to patient health by making the patient more susceptible to other diseases and often causes death by weakening the immune system and also affects cholesterol level. Based on these studies, it was moved toward the selection of Opuntia elatior as a herbal remedy for serum cholesterol level induced by gamma radiation. Methods: The modulatory effect of O. elatior stem extract was studied in Swiss albino mice at dose, i.e., 6 Gy of gamma radiation in the presence (experimental) or absence (control) of O. elatior extract (OEE) (10 mg/kg body weight) to observe in blood cholesterol level alterations. The animals were scarified and their blood was collected at days 1, 15, and 30 post-irradiation from heart puncture. Cholesterol level was estimated by the modified method of Roeschlau et al., 1974, using kit. Results: The cholesterol level was found almost constant in the control group (without any treatment) and increased in gamma radiation-treated group and decreased in only plant extract-treated group, whereas radiation-induced increased level of cholesterol was reduced by plant extract treatment in experimental animals (in plant extract with radiation-treated group), a normal level was achieved by day 30 post-treatment. Hence, O. elatior is beneficial to control radiation-induced blood cholesterol level. Conclusion: Radiation-induced side effects get lowered by herbal treatment and herbal products do not have their own side effects on body on their physical and chemical processes; thus, OEE is useful to manage blood cholesterol level as an edible part, it is easy to use as treatment and in my results OEE also support to maintain cholesterol level in serum of mice.

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Evaluation of the Effect of Hydroethanolic Root Extract and Solvent Fractions of Cyphostemma adenocaule (Steud. ex A. Rich) Descoings ex Wild & Drummond on Cell-Mediated Immune Response and Blood Cell Count in Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/1838903 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Dehnnet Abebe ◽

Gomathi Periasamy ◽

Aman Karim ◽

Helen Bitew

Keyword(s):

Blood Cell ◽

Cell Count ◽

Test Method ◽

Delayed Type Hypersensitivity ◽

Cell Mediated Immunity ◽

Root Extract ◽

Blood Cell Count ◽

Swiss Albino Mice ◽

Organ Index ◽

Albino Mice

Introduction. Cyphostemma adenocaule (Steud. ex A. Rich) Descoings ex wild & Drummond (Vitaceae) has been used in traditional medicine for the management of various immunological and hematological disorders in different areas of Ethiopia and the rest of the world. In Ethiopia, the plant is used for the management of enlarged spleen, rabies virus, helminthic infection, snake bite, and various types of tumors. Objective. To evaluate the effect of hydroethanolic root extract and solvent fractions of Cyphostemma adenocaule on cell-mediated immunity (delayed-type hypersensitivity), organ index (spleen and liver), and blood cell count in Swiss albino mice. Materials and Methods. Acute oral toxicity test was conducted using nulliparous and nonpregnant Swiss albino mice following OECD 425 limit test method. Delayed-type hypersensitivity model was used to evaluate the effect on cell-mediated immunity. The experimental animals were divided into twelve groups which were sensitized and challenged with sheep red blood cells on day 0 and day 7, respectively. Levamisole 50 mg/kg was used as stimulant control, whereas cyclophosphamide 30 mg/kg was used as suppressant control. Hydroethanolic root extract (100 mg/kg, 200 mg/kg, and 400 mg/kg), aqueous fraction (100 mg/kg, 200 mg/kg, and 400 mg/kg), and n-butanol fraction (100 mg/kg, 200 mg/kg, and 400 mg/kg) were administered for seven days. The paw volume was measured using a digital plethysmometer before challenge and 24 hours after challenge. Blood was collected, and organs (spleen and liver) were isolated from each challenged mouse to determine blood cell count and organ index, respectively. Results. No mortality and noticeable behavioral changes were observed among all mice receiving hydroethanolic root extract and solvent fractions at a dose of 2000 mg/kg. Hydroethanolic root extract and solvent fractions of Cyphostemma adenocaule showed enhancement of delayed-type hypersensitivity, organ index, and blood cell count. Hydroethanolic root extract at a dose of 400 mg/kg showed the highest and statistically significant stimulation of delayed-type hypersensitivity (0.123 ± 0.010) and blood cell count compared to the vehicle. Conclusion. Hydroethanolic root extract and solvent fractions of Cyphostemma adenocaule showed a stimulatory effect on cell-mediated immunity and hematopoiesis.

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Evaluation of In Vivo Antidiarrheal Activities of Hydroalcoholic Leaf Extract of Dodonaea viscosa L.(Sapindaceae) in Swiss Albino Mice

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2515690x19891952 ◽

2019 ◽

Vol 24 ◽

pp. 2515690X1989195

Author(s):

Jemal Abdela

Keyword(s):

Plant Extract ◽

Castor Oil ◽

Leaf Extract ◽

Gastrointestinal Transit ◽

Control Group ◽

Dodonaea Viscosa ◽

Swiss Albino Mice ◽

Antidiarrheal Activity ◽

Albino Mice

Traditionally people used Dodonaea viscosa for the treatment of various ailments, including diarrhea. Therefore, this study was aimed to evaluate the antidiarrheal activity of the 80% methanolic leaf extract of D viscosa against castor oil-induced diarrhea in mice models. Different doses of 80% methanolic leaf extract of D viscosa (100, 200, and 400 mg/kg) were evaluated for their antidiarrheal activities using castor oil–induced diarrhea, gastrointestinal transit, and enteropooling models in Swiss albino mice. At all test doses, the plant extract showed significant ( P < .05) inhibition in the frequency of defecation of wet feces and total fecal output as compared to the control group. Similarly, at all dose ranges used the plant extract demonstrated significant ( P < .05) reduction in an intraluminal fluid accumulation as compared to the untreated group. Besides, at higher doses, the plant extract also indicated significant ( P < .05) antimotility activity in comparison with the control. In conclusion, these findings illustrated that the 80% methanolic leaf extract of D viscosa supported the traditional claim of antidiarrheal activity of the plant though further investigations are warranted.

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Radioprotection of Swiss Albino Mice by Plant Extract Mentha piperita(Linn.).

Journal of Radiation Research ◽

10.1269/jrr.44.101 ◽

2003 ◽

Vol 44 (2) ◽

pp. 101-109 ◽

Cited By ~ 43

Author(s):

R. M. SAMARTH ◽

ASHOK KUMAR

Keyword(s):

Plant Extract ◽

Mentha Piperita ◽

Swiss Albino Mice ◽

Albino Mice

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IN VIVO ACUTE AND SUB-ACUTE TOXICITY STUDY OF ROOT EXTRACT OF CARISSA SPINARUM LINN. IN SWISS ALBINO MICE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2019v11i6.32369 ◽

2019 ◽

pp. 62-65

Author(s):

SIBHATU GEBREHIWOT

Keyword(s):

Body Weight ◽

Acute Toxicity ◽

Plant Extract ◽

Crude Extract ◽

Physical Parameters ◽

Swiss Albino Mice ◽

Experimental Animals ◽

Crude Plant Extract ◽

Albino Mice ◽

The Given

Objective: The objective of the present research work had been done to evaluate the toxicity of crude extract of Carissa spinarum in Swiss albino mice. Methods: In studying the toxicity, the Organization for Economic Cooperation and Development (OECD) guidelines were used. Experimental animals (mice), five mice in each, were grouped into four groups; three experimental groups and one negative control. In studying the acute toxicity, 2000, 3000 up to 5000 mg/kg crude plant extract was given orally using standard intragastric oral gavages. For acute toxicity, a single dose was given and gross behavioral changes were recorded. In sub-acute oral toxicity test, Carissa spinarum crude extract was given to the mice by standard intragastric oral gavages at doses of 500, 750 and 1000 mg/kg body weight of hydro-methanolic extract and 200, 600 and 1000 mg/kg of body weight of chloroform extract in every single to 28 d and various hematological and physical parameters were recorded. Results: In acute toxicity, the given dose of the plant extract did not produce significant physical and behavior changes up to the dose of 5000 mg/kg extracts. In addition, no death was occurred in the given doses. In sub-acute toxicity studies of the hydro-methanolic and chloroform extracts, there was no recorded significant change (p>0.05) of hematological and physical parameters in the treated groups when compared to the control groups. Conclusion: from the present study it was revealed that the crude extract of the plant did not produce any significant toxicological effect in the experimental animals and this supports the use of the plant in folk medicines.

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