Chemotherapeutic Effects of Docetaxel and Gene Expression of Epidermal Growth Factor Receptor During Regression of Mammary Tumours in Canines

2020 ◽  
Author(s):  
Prachi Upadhyay ◽  
N.S. Jadon ◽  
Priyanka Pandey ◽  
Jyotsana Bhatt ◽  
Ramanpreet Singh Sandhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Real Time ◽  
Real Time Pcr ◽  
Growth Factor Receptor ◽  
Mammary Tumour ◽  
Epidermal Growth ◽  
Group Ii

Background: In this study the chemotherapeutic effects of docetaxel and gene expression of epidermal growth factor receptor (EGFR) during regression of mammary tumour in canines were evaluated.Methods: Sixteen dogs suffering from canine mammary tumour were randomly divided into two groups viz. I and II and subjected to Docetaxel @ 30mg/m2 weekly, four consecutive cycles, (Group I) and surgical excision of tumour followed by chemotherapy with Docetaxel @ 30mg/m2 weekly, four consecutive cycles, (Group II). The therapeutic efficacy was assessed by clinical, radiological, ultrasonography, haemato-biochemical and histopathological evaluation including gene expression profiling of EGFR using Real-time PCR analysis.Result: On the basis of the above parameters studied it was concluded that combination of surgery and chemotherapy using docetaxel is an effective treatment for the regression of mammary tumour and can be used safely by the field veterinarian. Real-time PCR analysis also revealed down-regulation of EGFR gene in group II.

The epidermal growth factor receptor ligand amphiregulin is a negative regulator of hepatic acute-phase gene expression

2009 ◽  
Vol 51 (6) ◽  
pp. 1010-1020 ◽  
Author(s):  
Ana Pardo-Saganta ◽  
Maria Ujue Latasa ◽  
Josefa Castillo ◽  
Laura Alvarez-Asiain ◽  
María J. Perugorría ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Acute Phase ◽  
Growth Factor Receptor ◽  
Negative Regulator ◽  
Receptor Ligand ◽  
Epidermal Growth

scholarly journals Lycopene Inhibits Activation of Epidermal Growth Factor Receptor and Expression of Cyclooxygenase-2 in Gastric Cancer Cells

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 2113 ◽  
Author(s):  
Hwana Han ◽  
Joo Weon Lim ◽  
Hyeyoung Kim
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cancer Cells ◽  
Growth Factor Receptor ◽  
Ags Cells ◽  
Epidermal Growth ◽  
Cox 2

Reactive oxygen species (ROS) contribute to the oncogenic phenotype of cancer cells by acting as signaling molecules for inducing proliferation. ROS are known to activate the epidermal growth factor receptor (EGFR), which causes the activation of the Ras/mitogen-activated protein kinases (MAPKs) pathway. The Ras-dependent pathway promotes the activation of nuclear factor-kappa-light-chain-enhancer of activated B cells (NF-κB), a transcriptional modulator of cyclooxygenase-2 (COX-2) that induces cell proliferation. Lycopene is a potent antioxidant carotenoid and is responsible for the red color of fruits and vegetables. This study aims to investigate whether lycopene inhibits proliferation and induces apoptosis in gastric cancer AGS cells by suppressing the EGFR/Ras/MAPK and NF-κB-COX-2 signaling axis. Lycopene decreased cell viability and increased apoptotic indices (DNA fragmentation, apoptosis inducing factor, cleavage of caspase-3 and caspase-9, Bax/Bcl-2 ratio). Lycopene reduced the level of intracellular and mitochondrial ROS and decreased the activation of the ROS-mediated EGFR/Ras/extracellular signal-regulated kinase (ERK) and p38 MAPK pathways, thus leading to attenuation of the DNA-binding activity of NF-κB p50/p50 and the level of COX-2 gene expression. These results show that lycopene-induced apoptosis and inhibition of proliferation occur via inhibition of ROS-activated EGFR/Ras/ERK and p38 MAPK pathways and NF-κB-mediated COX-2 gene expression in AGS cells. In conclusion, consumption of lycopene-enriched foods could decrease the incidence of gastric cancer.

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