Anatomy of the Liver of Mizoram Local Pig (Zovawk)

2021 ◽  
Author(s):  
Swarup Debroy ◽  
P.C. Kalita ◽  
Arup Kalita ◽  
O.P. Choudhary ◽  
P.J. Doley ◽  
...  
Keyword(s):  
Animal Husbandry ◽  
Electron Microscopic Examination ◽  
Electron Microscopic Level ◽  
Average Weight ◽  
Free Ribosome ◽  
Structural Examination ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Transmission Electron ◽  
Highly Correlated

Background: The present study was aimed for the promotion and advancement of the anatomical knowledge at the gross, light microscopic and electron microscopic level in Zovawk (mizo local pig). Methods: The current investigation was done at the Instructional livestock farm, Department of Veterinary Anatomy and Histology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram and Sophisticated Analytical Instrument Facility (SAIF), North Eastern Hill University (NEHU), Shillong, Meghalaya. Six liver samples were collected from six apparently healthy Zovawk animal of either sex and gross morphological observations were done directly after collection. Thereafter tissue samples were collected as such and were preserved in neutral buffer formalin (NBF) and in Karnovsky’s fixative for routine histology and transmission electron microscopic examination, respectively.Result: Zovawk liver shows four distinct borders, i.e. medial, lateral, dorsal and ventral border, two surfaces, i.e. parietal surface or diaphragmatic surface and caudal or visceral surface and six distinct lobes. The average weight of Zovawk liver was 1.402 kg. Weight of the liver was highly correlated with body weight of animal. Histologically, Zovawk liver was characterized with thick Glisson’s capsule and thick connective tissue septa emerging from it, which gives the hepatic lobules its hexagonal shape. Sinusoids of adjacent hepatocytes were lined by stellate shaped Kupffer cells. The ultra-structural examination of liver shows that, the hepatocytes were rich in mitochondria, endoplasmic reticulum and glycogen granules. Free ribosome and well developed rER and dense lysosomal granules were common in those hepatocyte.

A Comparative Study of Fractographic Replicas

1974 ◽  
Vol 32 ◽  
pp. 566-567
Author(s):  
Loren Anderson ◽  
Pat Pizzo ◽  
Glen Haydon
Keyword(s):  
Electron Microscopy ◽  
Electron Microscopic Examination ◽  
Direct Examination ◽  
Electron Microscopic ◽  
Reliable Technique ◽  
Service Failures ◽  
Transmission Electron ◽  
Mode Of Failure ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Transmission electron microscopy of replicas has long been used to study the fracture surfaces of components which fail in service. Recently, the scanning electron microscope (SEM) has gained popularity because it allows direct examination of the fracture surface. However, the somewhat lower resolution of the SEM coupled with a restriction on the sample size has served to limit the use of this instrument in investigating in-service failures. It is the intent of this paper to show that scanning electron microscopic examination of conventional negative replicas can be a convenient and reliable technique for determining mode of failure.

scholarly journals Chromate Reduction in Serratia marcescens Isolated from Tannery Effluent and Potential Application for Bioremediation of Chromate Pollution

2002 ◽  
Vol 2 ◽  
pp. 972-977 ◽  
Author(s):  
M.A. Mondaca ◽  
V. Campos ◽  
R. Moraga ◽  
C.A. Zaror
Keyword(s):  
Natural Waters ◽  
Waste Treatment ◽  
Environmental Concern ◽  
Electron Microscopic Examination ◽  
Tannery Effluent ◽  
Chromate Reduction ◽  
Bacterial Cells ◽  
Electron Microscopic ◽  
Treatment Processes ◽  
Transmission Electron

Pollution of aquatic systems by heavy metals has resulted in increasing environmental concern because they cannot be biodegraded. One metal that gives reason for concern due to its toxicity is chromium. Cr(VI) and Cr(III) are the principal forms of chromium found in natural waters. A chromate-resistant strain of the bacterium S. marcescens was isolated from tannery effluent. The strain was able to reduce Cr(VI) to Cr(III), and about 80% of chromate was removed from the medium. The reduction seems to occur on the cell surface. Transmission electron microscopic examination of cells revealed that particles were deposited on the outside of bacterial cells. A stable biofilm was formed in less than 10 h, reaching around 1010cfu attached per milligram of activated carbon. These findings demonstrate that immobilizedS. marcescensmight be used in industrial waste treatment processes.

scholarly journals Disordered Myocardial Ca2+ Homeostasis Results in Substructural Alterations That May Promote Occurrence of Malignant Arrhythmias

2016 ◽  
pp. S139-S148 ◽  
Author(s):  
N. TRIBULOVA ◽  
V. KNEZL ◽  
B. SZEIFFOVA BACOVA ◽  
T. EGAN BENOVA ◽  
C. VICZENCZOVA ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Intact Animal ◽  
Electron Microscopic Examination ◽  
Atrial Pacing ◽  
Cardiac Cell ◽  
Cell Coupling ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
The Impact ◽  
Malignant Arrhythmias

We aimed to determine the impact of Ca2+-related disorders induced in intact animal hearts on ultrastructure of the cardiomyocytes prior to occurrence of severe arrhythmias. Three types of acute experiments were performed that are known to be accompanied by disturbances in Ca2+ handling. Langedorff-perfused rat or guinea pig hearts subjected to K+-deficient perfusion to induce ventricular fibrillation (VF), burst atrial pacing to induce atrial fibrillation (AF) and open chest pig heart exposed to intramyocardial noradrenaline infusion to induce ventricular tachycardia (VT). Tissue samples for electron microscopic examination were taken during basal condition, prior and during occurrence of malignant arrhythmias. Cardiomyocyte alterations preceding occurrence of arrhythmias consisted of non-uniform sarcomere shortening, disruption of myofilaments and injury of mitochondria that most likely reflected cytosolic Ca2+ disturbances and Ca2+ overload. These disorders were linked with non-uniform pattern of neighboring cardiomyocytes and dissociation of adhesive junctions suggesting defects in cardiac cell-to-cell coupling. Our findings identified heterogeneously distributed high [Ca2+]i-induced subcellular injury of the cardiomyocytes and their junctions as a common feature prior occurrence of VT, VF or AF. In conclusion, there is a link between Ca2+-related disorders in contractility and coupling of the cardiomyocytes pointing out a novel paradigm implicated in development of severe arrhythmias.

Electrophoretic and electron microscopic examination of the adductor and diductor muscles of an articulate brachiopod, Terebratalia transversa

1982 ◽  
Vol 60 (4) ◽  
pp. 550-559 ◽  
Author(s):  
William P. Eshleman ◽  
Jerrel L. Wilkens ◽  
Michael J. Cavey
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Electron Microscopic Examination ◽  
Light Chains ◽  
Electron Microscopic ◽  
Electrophoretic Patterns ◽  
Transmission Electron ◽  
Adductor Muscles ◽  
Regulation Of Contraction

The proteins of the striated adductor muscles, smooth adductor muscles, and diductor muscles of the articulate brachiopod Terebratalia transversa have been examined by sodium dodecyl sulfate – polyacrylamide gel electrophoresis. Electrophoretic patterns indicate the presence of paramyosin in all of these valve muscles. Tentative identification has also been made of the proteins responsible for actin and for myosin regulation of contraction (troponin–tropomyosin and myosin light chains, respectively). The myofilaments of the striated adductor cells, smooth adductor cells, and diductor cells have been characterized by transmission electron microscopy. The smooth adductor cells and the diductor cells exhibit very thick myofilaments which are fusiform in shape, exceptionally long, and axially banded. Morphological features of these thick myofilaments are consistent with those of paramyosin filaments found in other muscles and myoepithelia. Although the striated adductor cells contain paramyosin, it is not manifest in the thick myofilaments.

The effects of intravitreally injected bevacizumab on the retina and retina pigment epithelium: experimental in-vivo electron microscopic study in intact versus vitrectomized eyes

Open Medicine ◽  
2010 ◽  
Vol 5 (6) ◽  
pp. 745-751 ◽  
Author(s):  
Nilufer Kocak ◽  
Candan Ozogul ◽  
Suleyman Kaynak ◽  
Ulker Sonmez ◽  
Mehmet Zengin ◽  
...  
Keyword(s):  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Intravitreal Bevacizumab ◽  
Electron Microscopic Examination ◽  
Photoreceptor Cells ◽  
Control Group ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Electron Microscopes

AbstractTo analyze the retinal toxicity of bevacizumab at various doses both in vitrectomized and non-vitrectomized rabbit models. Twenty- eight rabbits were included in the study. Twenty- four rabbits were assigned to six groups, with 4 of the rabbits in the control group. The animals in Groups 1, 2 and 3 received bevacizumab at a dose of 0.3 mg, 0.5 mg and 1.5 mg /eye, respectively. The rabbits in Groups 4, 5 and 6 received intravitreal bevacizumab of 0.3 mg, 0.5 mg and 1.5mg/eye, respectively, after gas compression vitrectomy. Two weeks after the procedure, the rabbits were euthanized. Retina tissue samples were then obtained and examined with both light and electron microscopes. In Groups 1, 2 and 3 after bevacizumab injection, toxic degeneration in the photoreceptor and retinal pigment epithelium cells was observed via electron microscopic examination. The findings in Groups 4 and 5 were normal as compared to the control group. In Group 6, toxicity in the bipolar neurons and photoreceptor cells was noticed. Increased toxicity and retinal penetration were noticed in all administered doses of bevacizumab in the presence of vitreous. In addition, ocular toxicity occurred through the injection of the highest dose of bevacizumab after vitrectomy. It is possible that the bevacizumab dose and the, vitreous are as important as the drug half-life in the vitreous.

scholarly journals Internalization of Staphylococcus aureusby Endothelial Cells Induces Apoptosis

1998 ◽  
Vol 66 (12) ◽  
pp. 5994-5998 ◽  
Author(s):  
Barbara E. Menzies ◽  
Iordanka Kourteva
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Electron Microscopic Examination ◽  
Cytochalasin D ◽  
Nuclear Morphology ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Endothelial Cell Death ◽  
Umbilical Vein Endothelial Cells

ABSTRACT The ability of Staphylococcus aureus to invade and survive within endothelial cells is believed to contribute to its propensity to cause persistent endovascular infection with endothelial destruction. In the present study, we show that following invasion of human umbilical vein endothelial cells, intracellular S. aureus organisms remain viable over a 72-h period and, as determined by transmission electron microscopic examination, that the bacteria exist within vacuoles and free within the cytoplasm. We also demonstrate that endothelial cell death following S. aureusinvasion occurs at least in part by apoptosis as shown by DNA fragmentation and changes in nuclear morphology. Apoptotic changes were evident as early as 1 h after infection of endothelial cells. Internalization of S. aureus rather than adherence appears to be necessary, since use of the phagocytosis inhibitor cytochalasin D prevented apoptosis. UV-killed staphylococci, although retaining the capacity to be internalized, were not capable of inducing apoptosis, suggesting that apoptosis is dependent upon a factor associated with viable organisms. The studies demonstrate that viable intracellularS. aureus induces apoptosis of endothelial cells and that internalized staphylococci can exist free within the cytoplasm.

Ultrastructural studies of cartilage in genetic disorders of skeletal growth

1978 ◽  
Vol 36 (2) ◽  
pp. 668-669
Author(s):  
D. O. Sillence ◽  
D. L. Rimoin ◽  
Ruth Silberberg
Keyword(s):  
Genetic Disorders ◽  
Electron Microscopic Examination ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Electron Microscopic ◽  
Skeletal Dysplasias ◽  
Ultrastructural Studies ◽  
Low Viscosity ◽  
Transmission Electron ◽  
Cacodylate Buffer

The human skeletal dysplasias are an heterogeneous group of heritable connective tissue disorders associated with abnormalities in the size and shape of the limbs, trunk and/or skull which frequently result in disproportionate short stature. In recent years it has become apparent that these comprise over 50 distinct conditions with a variety of subtypes distinguished on clinical and radiological grounds. We have investigated the pathogenesis of these conditions in over 100 patients by direct transmission electron microscopic examination of chondro-osseous tissue. Some of the ultrastructural studies have been previously reported.Small biopsies of chondro-osseous junction were collected for electron microscopy from the rib or iliac crest of patients with skeletal dysplasias or from normal controls at the time of surgery. These were cut into small blocks and fixed for one hour in either 5% glutaraldehyde in white's buffer or directly in 1% osmic acid in White's buffer or a modified Karnovsky's fixative, (2. 5% paraformaldehyde, 2. 5% glutaraldehyde, 2. 5mM calcium in cacodylate buffer). Subsequent processing included osmium fixation, block staining with uranyl acetate and embedding in Araldite or Spurr's low viscosity resin (firm composition). Sections were cut with glass knives or diamond knives. The latter produced sections which were much more even in thickness, permitting more consistent appraisal of matrix features.

A Rapid Method for Electron Microscopic Examination of Biological Specimens

1971 ◽  
Vol 29 ◽  
pp. 486-487
Author(s):  
Glenn Stoner
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Simple Procedure ◽  
Contact Point ◽  
Electron Microscopic Examination ◽  
Test Solution ◽  
Biological Applications ◽  
Electron Microscopic ◽  
Preparation Time ◽  
Transmission Electron

The purpose of this paper is to demonstrate the use of a method which reduces the sample preparation time for transmission electron microscopy studies to about one minute.The relatively simple procedure is as follows: When a sample is desired, place 1 mℓ test solution in a 2-5 mℓ container, add 0.1 mℓ of a solution of 0.3 mg/mℓ fibrinogen, immediately insert a 400 mesh E.M. grid held by forceps, withdraw immediately, blot (at the tweezer tip-grid contact point) with filter paper, blow dry with a lab drier, add one drop of stain (2% urinal acetate), blot and blow dry in the above manner. Then immediately insert in the pre-pump chamber of the E.M.The above process has been used by the author in a variety of biological applications, from studies of fibrin growth from fibrinogen to identification of unknown viruses. The accompaning figures for T4, bacteriophage are given simply to demonstrate the method.

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