A Study on Some Serum Biological Constituents during Different Days of Normal Oestrous Cycle of Manipuri Pony Mares

Author(s):  
Ningthoukhongjam Linda ◽  
Fazal Ali Ahmed ◽  
J.K. Chaudhary ◽  
Mohammad Ayub Ali ◽  
Jagan Mohanarao Gali

Background: The aim of this study was to estimate the serum biochemical (glucose, cholesterol, total protein, calcium, phosphorus and magnesium) and hormonal constituents (estrogen and progesterone) in female Manipuri pony mares during the different days of oestrous cycle. Methods: A total of 50 numbers of blood samples were collected from 10 numbers of apparently healthy, non-pregnant on the different days of oestrous cycle i.e. day 0, 4, 8, 14 and 18 by using commercial diagnostic kits. Result: The mean serum levels of glucose, cholesterol and phosphorus were found to be non-significant on the different days of oestrous cycle whereas the mean serum level of total protein and calcium was found significantly different (P less than 0.01). The serum magnesium level differed significantly (P less than 0.05) amongst the different days of oestrous cycle. Both estrogen and progesterone were also found to be significant (P less than 0.01) between the different days of oestrous cycle. The results showed significant influence on serum biochemical and hormonal assay during different days of oestrous cycle in female Manipuri pony.

1992 ◽  
Vol 38 (10) ◽  
pp. 2002-2007 ◽  
Author(s):  
M Speich ◽  
A Murat ◽  
J L Auget ◽  
B Bousquet ◽  
P Arnaud

Abstract Concentrations of magnesium (Mg), total calcium (Ca), phosphorus (P), copper (Cu), and zinc (Zn) were investigated in plasma (Pl) and erythrocytes (Erc) of venous cord blood of 44 infants of diabetic mothers (IDMs). These same concentrations plus total glycohemoglobin and fructosamine were determined at delivery in a subset of 15 mothers of these infants. Mineral results for IDMs were compared with those for 66 apparently healthy newborns. The duration of gestation in the two groups was significantly different (P < 0.001). After adjustment for gestational age, the mean (+/- SD) differences between groups were significant for birth weight, head circumference, Erc-Mg (1.71 +/- 0.17 for IDMs vs 1.76 +/- 0.15 mmol/L for control subjects), Pl-Ca (1.96 +/- 0.32 vs 2.48 +/- 0.22 mmol/L), Pl-P (1.99 +/- 0.40 vs 1.57 +/- 0.25 mmol/L), and Erc-Cu (10.9 +/- 2.41 vs 12.9 +/- 3.00 mumol/L), but not for Erc-Zn (33.0 +/- 18.3 vs 40.4 +/- 13.6 mumol/L). The variable that best discriminated between the two infant groups after adjustment for gestational age was Pl-Ca. In the 15 mothers, Pl-Mg (0.67 +/- 0.07 mmol/L) and Pl-Ca (1.66 +/- 0.21 mmol/L) concentrations were low, Pl-Zn (9.81 +/- 3.40 mumol/L) was normal, and Pl-Cu (33.5 +/- 10.7 mumol/L) was above normal. Correlations between total glycohemoglobin and mineral values of the mothers or paired IDM mineral values were not significant. The concentration of Pl-Ca was positively correlated with Erc-Cu (P < 0.001) and Pl-Cu (P < 0.05) in the comparison group newborns but not in the IDMs.


2018 ◽  
Vol 10 (1) ◽  
pp. 10-15
Author(s):  
HL Roy ◽  
S Nargis ◽  
SH Mahmud ◽  
NWB Jahan ◽  
MS Hossain ◽  
...  

Preeclampsia is the most common medical complication of pregnancy associated with increased maternal and infant mortality and morbidity. Reduced serum magnesium level is found to be associated with elevated blood pressure in preeclampsia. The purpose of this cross-sectional study is to evaluate the serum magnesium level in preeclamptic women. For this study 50 preeclamptic patients, with age range 20 to 40 years having gestational age range from 20 to 40 weeks and 50 age and gestational age matched normotensive pregnant women having no proteinuria were enrolled. Serum magnesium was measured by colorimetric method. The mean(±SD) age and mean(±SD) gestational age of preeclampsia was not significantly different from those of normotensive pregnant women (p=0.203 and p=0.251 respectively). The mean(±SD) body mass index of the preeclamptic patients was significantly higher compared to that of normotensive pregnant women (p<0.001). The mean(±SD) serum magnesium level was 3.37±2.05 mg/dl in preeclamptic group and 2.87±1.38 mg/dl in normal pregnant women; the level did not differ significantly between the subject of preeclampsia and normal pregnant women (p=0.153). Findings of this study suggest that serum magnesium has no association with preeclampsia.Bangladesh J Med Biochem 2017; 10(1): 10-15


2021 ◽  
Vol 8 (35) ◽  
pp. 3224-3229
Author(s):  
Jasleen Kaur ◽  
Satya Bhushan Nayyar ◽  
Tejinder Sikri ◽  
Jasmine Kaur ◽  
Hardeep Singh Deep

BACKGROUND World Health Organization (WHO) clinically defines a stroke as ‘the rapid development of clinical signs and symptoms of a focal neurological disturbance lasting more than 24 hours or leading to death with no apparent cause other than vascular origin’. Hypo magnesia (ionized form) leads to neuromuscular hyperirritability, tremors, increased vascular resistance, coronary vasospasm and hypertension. Magnesium deficiency triggers vasoconstriction enhancing vascular endothelial injury and hence leads to atherosclerosis. In the present study, we wanted to evaluate serum magnesium levels in ischemic cerebral stroke patients and correlate its severity with the neurological disability using modified Rankin scale (mRS) and Canadian neurological scale. METHODS It was a longitudinal observational study, undertaken in the Department of Medicine in Sri Guru Ram Das Institute of Medical Sciences and Research, Sri Amritsar from December 2018 to June 2020. 60 patients with acute ischemic cerebral stroke fulfilling the inclusion criteria were selected. 5 ml venous sample for serum magnesium level was taken within 24 hours and on day 5 of admission. Presence or absence of hypomagnesemia in patients after acute cerebral ischemic stroke was recorded during the hospital stay of patients, receiving standard management protocol of ischemic stroke. The correlation co-efficient of serum magnesium level with modified Rankin scale and Canadian neurological scale was calculated. RESULTS The mean age of patients was 61.6 ± 1.6 years, 42 (70 %) patients were male and 18 (30 %) were females and there was male preponderance. The mean serum magnesium level was measured as 1.78 ± 0.2 mg/dL, it was observed that the mean value for mRS was 3.93 ± 0.75 and Canadian neurological scale was 7.11 ± 2.01 within 24 hours and on the 5th day the mean value for mRS was 3.5 ± 1.09 and Canadian neurological scale was 8.02 ± 2.97. The present study observed a statistically significant correlation between mRS score and serum magnesium level as well as Canadian neurological scale and serum magnesium levels. CONCLUSIONS It was concluded through the results of this study that low levels of magnesium in the body can cause more severe stroke. KEYWORDS Stroke, Serum Magnesium, Hypomagnesemia, Modified Rankin Scale, Canadian Neurological Scale


2019 ◽  
Vol 71 (1) ◽  
pp. 187-196 ◽  
Author(s):  
D.C. Souza ◽  
D.G. Silva ◽  
T.G. Rocha ◽  
B.M. Monteiro ◽  
G.T. Pereira ◽  
...  

ABSTRACT Serum blood samples from 50 Murrah buffalo calves were examined in this study. The animals were allocated into three groups according to the number of parturitions of their mothers: G1 (n= 15) calves from primiparous buffaloes, G2 (n= 19) calves from buffaloes with two to four parturitions, and G3 (n= 16) calves from buffaloes with five or more parturitions. Blood samples were taken at birth, before colostrum ingestion, at 24h, 48h, and 72h after birth, and at 7, 14, 21, and 30 days after birth for determination of levels of gammaglutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), creatine kinase, total protein, albumin, globulins (including immunoglobulin G), iron, total calcium, ionized calcium, phosphorus, sodium, and potassium. The age of the calves was found to influence all of the biochemical parameters, with the exception of ionized calcium and potassium in the calves in groups G1 and G3. The calving order was found to influence AST, GGT, total protein, albumin, and globulins, including IgG. The high serum ALP activity in the first two days after birth indicates that measurement of the levels of this enzyme may be used as an indirect method of assessing passive immunity transfer.


2018 ◽  
Vol 14 (12) ◽  
pp. 437
Author(s):  
Allagoa DO. ◽  
Agbo OJ. ◽  
Orluwene CG.

Background: Calcium and magnesium are two micronutrients whose role in the development of preeclampsia has been investigated over the years. There is paucity of studies on the role of serum levels of calcium and magnesium in the development of preeclampsia in south-south Nigeria. Objective: Our study evaluated the serum level of calcium and magnesium in preeclamptic and non preeclamptic women in a Tertiary Hospital in southsouth Nigeria. Methodology: We carried out a comparative study in which 52 preeclamptic and 52 non preeclamptic women, who satisfied the eligibility criteria, were enrolled for the study. Data entry and statistical analysis was done using statistical software (IBM SPSS® for windows version 21.0). Data were analyzed for mean and standard deviation. Comparison of serum levels of elements between the two groups was performed by student t-test, and P value < 0.05 was considered as statistically significant. Results: The serum calcium level was statistically lower in the women who developed preeclampsia compared to those who did not (8.37±0.91 mg/dl vs 9.33±1.15mg/dl, p<0.001). The serum magnesium level was not statistically different between women who had preeclampsia and those who did not (1.79±0.24mg/dl vs 1.88±0.37mg/dl, p = 0.102). The systolic and diastolic blood pressure showed a significant negative correlation with serum calcium level, unlike serum magnesiun level in those that had preeclampsia. Conclusion: This study showed that women who developed preeclampsia demonstrated reduced serum calcium level and no reduction in serum magnesium level. This study support the hypothesis that hypocalcaemia, unlike hypomagnecaemia, is a possible aetiology of preeclampsia.


2021 ◽  
Vol 33 (1) ◽  
pp. 58-60
Author(s):  
Heera Lal Roy ◽  
Susmita Nargis ◽  
Farhana Ferdaus

Introduction: There are many Hypertensive disorders in pregnancy like preeclampsia, eclampsia etc. Pre-eclampsia is the most common medical complication of pregnancy associated with increased maternal and infant mortality and morbidity. Some studies have implicated that low serum calcium levels may have a role in pre-eclampsia but other studies failed to find relation between low levels of these trace elements and pre-eclampsia. Materials and Methods: This cross sectional study was carried out in the Department of Biochemistry, Sylhet MAG Osmani Medical College in collaboration with the Department of Obstetrics and Gynaecology, Sylhet MAG Osmani Medical College Hospital during the period from January 2016 to December 2016. This Study was occured in among 31 pre-eclampsia patients, aged 20 to 40 years, and gestational age ranges from 20 to 40 weeks and 31 age matched normotensive non-pregnant women having no proteinuria. Serum magnesium was measured by Colorimetric method. Results: The mean serum magnesium level was 3.24 (±1.42) mg/dl in pre–eclampsia and was 3.30 (±1.5) mg/dl in normal women. The mean serum magnesium level did not differ significantly between the subjects of pre–eclampsia and normal women (t=0; p<.05). Conclusion: The means of both data sets are equal. So we can conclude that there is no significant difference between them. Medicine Today 2021 Vol.33(1): 58-60


Author(s):  
Ezeugwunne Ifeoma Priscilla ◽  
Chukwu Goodness Nkechinyere ◽  
Ogbodo Emmanuel Chukwuemeka ◽  
Analike Rosemary Adamma ◽  
Onyegbule Onyema Athanatius ◽  
...  

This study investigated the effect of short term aerobic exercise on lipid profile and myoglobin levels in apparently healthy students of College of Health Sciences, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria. A total of 60 apparently healthy young students aged between 18-40 years were randomly selected for the study. They were rested for a period of two weeks after which they were subjected to football exercise for 30 minutes (3 times) in a week for a period of 3 weeks. The demographic and anthropometric data of subjects were obtained using a well-structured questionnaire. Their blood pressures reading were obtained also. Thereafter, 5mls of baseline (day 0) and post-exercise (day 21) blood samples were collected from each subject before the commencement and at the end of the exercise on the last day of the training into a plain container for estimation of biochemical parameters (TG, TC, LDL, HDL, Myoglobin levels) respectively using standard methods. Data obtained were statistically analysed by unpaired student t-test and Pearson r correlation. The result showed that the mean serum levels of TG (1.41±0.60 vs 1.04±0.43) TC (5.22±0.98 vs 4.41± 0.90), LDL (3.92±0.99 vs 3.36±0.92) as well as myoglobin (16.04± 4.64 vs 13.38±5.21) and DBP (75.16±10.54 vs 68.59±8.57) were significantly decreased, whereas, there was a significant increase in the mean serum level of HDL (0.83± 0.30 vs 0.95±0.28) and SBP (114.69±9.21 vs 131.48±12.84) in subjects after exercise when compared to before exercise (p<0.05). However, the mean BMI of the subjects remained the same before and after exercise (p>0.05). Therefore, short term moderate intensity aerobic exercise could be of importance in prevention and management of cardiovascular disease


2012 ◽  
Vol 36 (0A) ◽  
pp. 77-85
Author(s):  
Raad Mahmood Hussein

This study was conducted on local breed goats to determine the changes in biochemical parameters in anemic cases by using morphological classification, one hundred and seventyserum samples were collected randomly from goats of both sexes aged 3 months – 6 years, they were grazing on pasture from the different districts of Baghdad, Bakuba and Falluja. The total protein, albumin and fibrinogen in the normal bucks were 73 1 – 84 1 g/L, 35 3- 37 1 g/ L, 9 8- 11 1 g/ L, respectively and in the normal does were 66 1 – 77 4 g/ L, 35 6 – 37 2 g/ L and 6 – 15 g/ L, respectively, the average of these parameters differed significantly at (p> 0.05) between the normal and the anemic animals. The mean level of copper in the serum of normal goats was 21.66 µmol/L and the the mean level of iron in serum was 29.24 µmol/L these averages value were declined in the anemic goats was significantas compared with apparently healthy goats.


2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Takayuki Fujii ◽  
JUNYA KOSHIZAKA ◽  
NOBUAKI YAMAUCHI ◽  
MAYU MORIMOTO ◽  
NORIKO TERASAKI ◽  
...  

Abstract Background and Aims Serum magnesium levels are an important factor associated with vital prognosis, cardiovascular disease, and vascular calcification in hemodialysis patients. Moreover, low serum magnesium levels are reportedly associated with an increased risk of hip fractures. Previously we reported that mean magnesium level during the first 3 years of hemodialysis was associated with all-cause mortality in ERA-EDTA 2018. Here, we investigated whether mean magnesium level during the first 3 years of hemodialysis can predict fracture risk. Method A retrospective cohort study was conducted in 283 patients who started hemodialysis at our hospital between March 2004 and the present and who could be followed for at least 3 years or had a fracture within 3 years. To assess the association between mean serum magnesium level during 3 years after the start of hemodialysis or until fracture and fracture risk, a multivariate analysis was performed using the Cox proportional hazard model with factors related to fractures as covariates, with all fractures such as hip fracture and spinal compression fracture as the outcome. Results The mean follow-up period was 6.1 years, and the mean age of patients was 64 years, with women accounting for 31%. Of all 283 patients, 62 developed fractures. A univariate analysis of factors associated with fractures revealed that high serum magnesium level was a significant fracture suppressor (hazard ratio [HR]: 0.31 [0.12–0.73]). Multivariate analysis conducted with significant factors in univariate analysis (mean serum albumin level, serum phosphorus level, hemoglobin level, C-reactive protein level, age, history of heart disease, history of bone fractures, and use or nonuse of phosphate binder during the 3 years) as the covariates revealed that the serum magnesium level was not significantly associated with bone fractures (HR: 0.95 [0.33–2.78]), whereas, old age, history of fractures, low albumin level, high CRP level, and nonuse of phosphate binder were significantly associated with fractures. Conclusion Serum magnesium levels during 3 years after the initiation of hemodialysis did not predict the risk of subsequent fractures, whereas a high level of inflammatory response, malnutrition, and nonuse of phosphate binder did.


2020 ◽  
Vol 14 (2) ◽  
pp. 71-73
Author(s):  
Mohammad Rezaul Quader ◽  
Sharmin Rahman ◽  
Nasima Sultana ◽  
Suranjit Kumar Saha

Dyslipidemia is an established risk factor of acute myocardial infarction (AMI), but measurement of macro metals like magnesium can be helpful in the prevention and better management of AMI. The aim of this study was to estimate serum magnesium in AMI. This is a case control type of study carried out in the Department of Biochemistry, Dhaka Medical College, Dhaka during the period of January 2015 to December 2015 with a total number of 100 study subjects. Acute myocardial infarction patients were selected as case (50) from coronary care unit (CCU), Department of Cardiology, Dhaka Medical College Hospital. Normal healthy individuals were selected as control (50) from the attendants of patients, relatives and doctors. Serum level of magnesium were assessed for both case and control groups. The mean values of the variable were compared between them by statistical analysis using SPSS version 16. For all the statistical analysis P<0.05 was considered as significant. The mean values of serum magnesium were 1.63±0.27mg/dl in cases. The mean values of serum magnesium were 2.35±0.28 mg/dl in control group. Significant differences were found in mean values between case and control groups and differences were highly significant (p<0.001). In AMI, serum magnesium level was found to be lower in this study. Serum magnesium is an important trace element that act as cofactor in many biochemical reactions. Decrease level of this important trace element may contribute to pathogenesis of AMI. So with other biochemical risk parameters, routine assessment of serum magnesium level is advocated, which might be helpful for prevention and better management of AMI. Faridpur Med. Coll. J. Jul 2019;14(2): 71-73


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