Efficient application of Trichoderma viride on soybean [Glycine max (L.) Merrill] seed using thin layer polymer coating

2018 ◽  
Author(s):  
P. Falah Kuchlan ◽  
M.M.M. Ansari K. Kuchlan ◽  
M. M. Ansari
Keyword(s):  
Polymer Coating ◽  
Seed Treatment ◽  
Disease Incidence ◽  
Trichoderma Viride ◽  
Agar Plate ◽  
Solution Treatment ◽  
Soybean Seed ◽  
Culture Solution ◽  
Seedling Mortality ◽  
Talcum Powder

Soybean crop is very sensitive to change in climate and suffers from vagaries of climate. The productivity thus gets affected in different years due to the influence of climatic condition, incidence of diseases and insects. Trichoderma sp. is considered as a potential biocontrol and growth promoting agents for many crop plants. Trichoderma spp. is generally being applied to the soil and seed as talcum powder form. Though powder formulations may be applied directly to seeds at the rate of 5g/kg seed, the effectiveness of Trichoderma seed treatment is lost due to poor adhesion on the smooth seed coat surface of soybean. Thus, it was targeted to achieve maximum benefit by Trichoderma seed treatment mediated through polymer coating. Two soybean cultivars were treated with Trichoderma viride with different formulation namely Trichoderma culture solution with polymer, Trichoderma culture talc with polymer, Trichoderma culture talc powder dry dressing. Endophytic growth of Trichoderma viride in root, stem and leaf was studied by agar plate method at 27±10C for seven days. The percentage of plants with Trichoderma endophytic growth was 97-100 per cent in plants from seeds treated with Trichoderma with polymer as compared to 37-45 per cent in plants from seeds treated with Trichoderma talc powder dry dressing. Control plants did not show any endophytic growth of Trichoderma. Significant increase in plant height was observed due to endophytic growth of Trichoderma. 24 per cent yield advantage was observed due to Trichoderma culture solution treatment with polymer. Seedling mortality due to collar rot (0.61%) and disease incidence of Myrothecium Leaf Spot, Anthracnose and Rhizoctonia Arial Blight (0.34%) was significantly lower than control (9.8% and 9.169%). Proper application of Trichoderma viride on soybean seed through polymer coating has significant potential to reduce diseases and to improve plant growth and seed yield.

Eco-friendly management of wilt caused by fusarium oxysporum f.sp. Ciceri in chickpea

2015 ◽  
Author(s):  
Chandar Kala ◽  
S. Gangopadhyay ◽  
S. L. Godara
Keyword(s):  
Population Dynamics ◽  
Disease Control ◽  
Fusarium Oxysporum ◽  
Seed Treatment ◽  
Disease Incidence ◽  
Trichoderma Viride ◽  
Organic Amendments ◽  
Chickpea Wilt ◽  
Maximum Inhibition

Antagonistic potentiality of Trichoderma viride, T. harzianum and Pseudomonas fluorescens were evaluated against Fusarium oxysporum f. sp. ciceri under in vivo conditions. The effect of organic amendments viz; farm yard manure, vermicompost and mustard cake on disease control potentiality of test antagonists against chickpea wilt and on population dynamics of the antagonists and pathogen in soil was also studied. Maximum inhibition of mycelial growth of F. o. f. sp. ciceri was recorded in presence of P. fluorescens (%) followed by T. harzianum (%) and T. viride (%). Seed treatment with P. fluorescens was more effective in suppressing the disease incidence as compared to T. harzianum and T. viride. The disease control efficacy and population dynamics of all the three test antagonists was enhanced in response to application of organic amendments. Among the three organic amendments tested, mustard cake was most effective in enhancing the disease control potentiality of these antagonists.

scholarly journals Eco-friendly management of major diseases in jute (Corchorus olitorius L.)

2014 ◽  
Vol 6 (2) ◽  
pp. 541-544 ◽  
Author(s):  
P. N. Meena ◽  
A. Roy ◽  
B. S. Gotyal ◽  
S. Mitra ◽  
S. Satpathy
Keyword(s):  
Seed Treatment ◽  
Disease Incidence ◽  
Block Design ◽  
Trichoderma Viride ◽  
Corchorus Olitorius ◽  
Farm Yard Manure ◽  
Soil Application ◽  
Randomized Block Design ◽  
Fibre Yield ◽  
Cent Disease

Field experiment was conducted for disease management with seven eco-friendly treatments in Corchorus olitorius jute during 2012-2013 under randomized block design (RBD) in three replications with variety JRO-524. The ecofriendly treatment 50% N: P: K + seed treatments with Azotobacter and phosphorus solubilizing bacteria (PSB) @ 5g/Kg+ Trichoderma viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha at 21DAS) + Psuedomonas fluorescens spray @ 0.2% at 45DAS was found superior with minimum per cent disease incidence (3.46 and 1.43%) as compared to control (13.17 and 4.96%). The second best treatment was found with Farm yard manure (FYM) @ 5t/ha + seed treatment with Azotobecter and PSB @ 5g/Kg+ T. viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha 21DAS)+ P. fluorescencs spray @ 0.2% at 45DAS and showed 3.61 and 2.0% disease incidence. The treatment with N: P: K @ 60:30:30 and seed treatment with carbendazim 50WP @ 2g/Kg at 45DAS showed 4.56 and 2.10 % disease incidence. Dry fibre yield was highest (30.33q/ha) in the plots treated with FYM @ 5t/ha + seed treatment with Azotobacter and PSB @ 5g/Kg+ T. viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha at 21DAS) + P. fluorescencs spray @ 0.2% at 45DAS) and it was lowest in untreated control (15.69 q/ha).Variation in yield was attributed due to difference in plant height.

scholarly journals Integrated Disease Management for Pigeonpea Wilt Caused by Fusarium udum

2019 ◽  
Author(s):  
RL Sharma ◽  
Tushar Mishra ◽  
Rakesh Bhagat ◽  
Vivek Swarnkar
Keyword(s):  
Seed Yield ◽  
Seed Treatment ◽  
Field Experiments ◽  
Integrated Management ◽  
Disease Incidence ◽  
Trichoderma Viride ◽  
Germination Percentage ◽  
Wilt Disease ◽  
Integrated Disease Management ◽  
Wilt Incidence

Field experiments were conducted during Kharif seasons of 2013 and 2014 in the farmer's field of Gariyaband, Chhattisgarh, India to find out different integrated management levels of control schedule for pigeonpea wilt disease. All treatments had a significant effect on germination percent, disease incidence, seed yield and disease index in both the consecutive years of experimentation. T4–Seed treatment with Thiram + Carbendazim+ Trichoderma viride + Rhizobium + Soil application of Trichoderma viride, resulted in higher germination percentage (96.8 and 97.2), wilt incidence percent at 60 DAS (2.97 and 3.15), wilt incidence per cent at 150 DAS (9.68 and 7.65) and seed yield (15.10 and 16.28 q ha-1) during 2013 and 2014, respectively and was found superior over rest of the treatments. The loss in pigeonpea yield was up to the tune of 18.86 to 54.24% due to the incidence of wilt disease.

scholarly journals Chitosan for suppression of fusarium wilt and plant growth promotion of brinjal

2021 ◽  
Vol 2 (2) ◽  
pp. 124-137
Author(s):  
S. Chaterjee ◽  
Rayhanur Jannat ◽  
M. M. Hossain ◽  
M. R. Amin ◽  
M. T. Rubayet
Keyword(s):  
Plant Growth ◽  
Fusarium Wilt ◽  
Soil Amendment ◽  
Seed Treatment ◽  
Disease Incidence ◽  
Block Design ◽  
Plant Diseases ◽  
Growth And Yield ◽  
Pathogenicity Test ◽  
Seedling Mortality

Chitosan is a biodegradable natural compound that has a great potentiality in agriculture for controlling plant diseases. An attempt was made to control Fusarium wilt caused by Fusarium oxysporum f. sp. melongenae under inoculated field condition and increase the growth and yield of brinjal by chitosan. Before setting the experiments in the field, preliminary laboratory experiments were carried out to select virulent isolate and effective dose of chitosan against the mycelial growth of the selected pathogen. F. oxysporum f. sp. melongenae isolate F-1 was found to be the most virulent on brinjal in pathogenicity test. Chitosan @ 1.0% concentration was appeared to be the highest inhibitory to the test pathogen at in vitro condition. Additionally, seed treatment with 1.0% chitosan for 12 hrs resulted in the highest increased in germination and seedling growth of brinjal. The field experiment was conducted following Randomized Complete Block Design (RCBD) with four treatments. No treatment was given in T1, the pathogen was inoculated in T2 and seed treatment and soil amendment with 1.0% chitosan was done in T3 and T4, respectively, in test pathogen inoculated condition. Application of 1.0% chitosan as a seed treatment (T3) or soil amendment (T4) significantly reduced pre- and post-emergence seedling mortality, incidence and severity of Fusarium wilt as well as enhanced germination percentage, plant growth and yield of brinjal. On the contrary, pre-emergence and post-emergence seedling mortality, disease incidence and severity of Fusarium wilt were highest in treatment T2 where the soil was inoculated with pathogen without chitosan. Therefore, chitosan could be used against this vascular disease as an alternative to inorganic fungicides and augment yield.

scholarly journals Evaluation of Different Measures to Control Wilt Causing Pathogens in Chickpea

2011 ◽  
Vol 51 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Muneeb Andrabi ◽  
Amrish Vaid ◽  
Vijay Razdan
Keyword(s):  
Seed Treatment ◽  
Cultural Practices ◽  
Disease Incidence ◽  
Trichoderma Viride ◽  
Maximum Yield ◽  
Row Spacing ◽  
Trichoderma Virens ◽  
Late Sowing

Evaluation of Different Measures to Control Wilt Causing Pathogens in Chickpea Fusarium oxysporum f. sp. ciceri, F. solani and Rhizoctonia solani were isolated from the wilted chickpea (Cicer arietinum) plants. To manage the wilt complex cultural practices, use of biocontrol agents and fungicides were tried under in vitro and in vivo conditions. Sowing of chickpea at different dates revealed that early sowing (10th Oct.) resulted in maximum disease incidence (32.20%), whereas, late sowing (24th Nov.) the minimum (13.35%). Twenty and 50 cm row to row spacing resulted in maximum (29.17%) and minimum (17.35%) disease incidence respectively. In vitro evaluation of biological control agents revealed the superiority of Trichoderma viride. Trichoderma over Trichoderma virens in controlling the pathogens. Carbendazim at 100, 200, 500 ppm caused maximum per cent inhibition of the pathogens under in vitro conditions. Fungicides applied as seed treatment reduced disease incidence significantly. Seed treatment with carbendazim increased seed germination (71.24%), though it was at par with carbendazim + mancozeb (62.21%) and mancozeb (61.46%). Seed coating with T. viride resulted in minimum disease incidence (9.24%), however, it was at par with T. virens (9.72%). Maximum yield (10.10 q/ha) was recorded with the application of carbendazim, followed by carbendazim + mancozeb (9.77 q/ha) and T. viride (8.10 q/ha).

scholarly journals Efficiency of Bio-fungicides (Trichoderma spp and Pseudomonas fluorescens) on Seedling Emergence, Vigour and Health of Infected Chilli Seeds (Capsicum annuum) by Colletotrichum capsici

2019 ◽  
pp. 1-8
Author(s):  
Y. N. Priya Reddy ◽  
S. S. Jakhar ◽  
O. S. Dahiya
Keyword(s):  
Pseudomonas Fluorescens ◽  
Seed Treatment ◽  
Negative Impact ◽  
Disease Incidence ◽  
Seedling Emergence ◽  
Trichoderma Viride ◽  
Fruit Rot ◽  
Seedling Vigour ◽  
Colletotrichum Capsici ◽  
Pot Culture

Damping off and fruit rot caused by Colletotrichum capsici are the major constraints in production and marketability of chilli. Systemic fungicides are commonly used to control this disease. However, continuous use of chemical fungicides leads to negative impact on environment, soil and human health. Therefore, present studies (blotter and pot experiment) were conducted to explore the bio-fungicides (as an alternative to chemical fungicide) in comparison with carbendazim using chilli seeds infected with Colletotrichum capsici. Experiments were conducted at the CCSHAU, Hisar, India during 2016 in completely randomized design with nine treatments replicated three times. Six months old seeds having germination above the Indian Minimum Seed Certification Standard, were infected with Colletotrichum capsici and such infected seeds were treated with Trichoderma asperellum, Trichoderma viridae, Pseudomonas fluorescens individually and their combinations to control the disease incidence. The infected, un-infected and seed treatment with carbendazim served as controls. Results revealed that the seed germination was significantly higher (94.7%) with Trichoderma viride treatment compared to all other treatments including controls in blotter method. However, the seedling emergence in pot culture was significantly superior with Carbendazim treatment, the seed treatment with Pseudomonas fluorescens and Trichoderma viridae was on par to that of Carbendazim treatment. The seedling length was significantly superior with Trichoderma viride compared to the carbendazim and other controls both in blotter and pot culture. The seedling dry weight and seedling vigour were significantly higher with carbendazim as compared to the Trichoderma viride treatment or other treatments in both blotter and pot culture. However, the overall seedling vigour obtained with Trichoderma viride was similar to that of carbendazim treatment. The disease incidence was significantly lower with Pseudomonas fluorescens as compared to the Trichoderma viride and carbendazim in blotter method and; T. viride + P. fluorescens treatment was on par to that of carbendazim treatment in pot culture. Therefore, use of Trichoderma viride and Pseudomonas fluorescens individually or in combination are suggested as an alternative to carbendazim to control the Colletotrichum capsici.

On-Farm Soybean Seed Treatment Trials

2015 ◽  
Author(s):  
Jim Fawcett ◽  
Zack Koopman ◽  
Lance Miller ◽  
Wayne Roush ◽  
Josh Sievers
Keyword(s):  
Seed Treatment ◽  
Soybean Seed ◽  
Treatment Trials ◽  
On Farm

Tyrosol, a phenolic compound from Phomopsis sp., is a potential biostimulant in soybean seed treatment

2021 ◽  
Vol 43 ◽  
pp. 40-44
Author(s):  
Geraldo Humberto Silva ◽  
Josiane Abadia Resende de Souza ◽  
Willian Rodrigues Macedo ◽  
Frederico Garcia Pinto
Keyword(s):  
Phenolic Compound ◽  
Seed Treatment ◽  
Soybean Seed

scholarly journals Estimation of interrelationships among some quality factors of jute seeds

2016 ◽  
Vol 25 (1) ◽  
pp. 9-17
Author(s):  
Md Al Mamun ◽  
S Shamsi ◽  
MA Bashar
Keyword(s):  
Seed Germination ◽  
Negative Correlation ◽  
Agar Plate ◽  
Curvularia Lunata ◽  
Rhizopus Stolonifer ◽  
Quality Factors ◽  
Seedling Mortality ◽  
Corchorus Capsularis ◽  
Paper Towel ◽  
Blotter Paper

The storage mycoflora associated with seeds of 21 accessions of Corchorus capsularis L. were isolated and identified. Blotter, Paper towel and Agar plate methods were used to isolate the fungi associated with seeds. A total of ten species of fungi viz. Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Curvularia lunata, Colletotrichum corchori, Fusarium oxysporum, Eurotium sp., Penicillium sp. and Rhizopus stolonifer were isolated from the selected jute seeds. Aspergillus spp. and Colletotrichum corchori were predominant in most of the jute accessions. Accession No. A-3047 showed the highest fungal incidence and Accession Nos. A-3361, A-2734 and A-877 showed the lowest. Accession No. A-3361 showed the highest seed germination while Accession No. A-3309 showed the lowest. Mortality of seedling showed the highest in Accession No. A-3309 and the lowest in Accession No. A-3361. Physical purity of seed recorded the highest in Accession No. A- 3361 and the lowest in Accession No. A-3309. There were negative correlation between percentage of fungi and percentage of germination and between percentage of fungi and physical purity of seeds. Positive correlation was observed between percentage of fungi and seedling mortality and between purity of seed and germination of seed.Dhaka Univ. J. Biol. Sci. 25(1): 9-17, 2016

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