Effect of increasing concentration of paracetamol on performance of Vigna radiata: A concentration dependent study

2018 ◽  
Author(s):  
Ibrahem A.A> Almohisen
Keyword(s):  
Vigna Radiata ◽  
Mung Bean ◽  
Growth And Development ◽  
Growth Traits ◽  
Distilled Water ◽  
Dependent Manner ◽  
Water Control ◽  
Concentration Dependent Manner ◽  
Germination And Growth

In the recent times, it was shown that panadol (paracetamol) are involved in the processes of growth and development of plants. With this motion the present work was undertaken to explore the effect of panadol treatment on germination and various growth biomarkers of 30-days-old plants of mung bean (Vigna radiata). At 10 days stage of growth, seedlings were sprayed with distilled water (control), 0.0001, 0.0005, 0.001, 0.005, 0.01, 0.1 mg/L of panadol for 10 days. Treated plants were harvested at 30 days of growth to asses various parameters. The plants that developed from the panadol treatment demonstrated improved germination and growth traits in a concentration dependent manner. Out of the various concentrations (0.0001, 0.0005, 0.001, 0.005, 0.01, 0.1 or 1.0 mg/L) of panadol tested, 0.1 mg/L proved to be the best.

scholarly journals Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1311
Author(s):  
Magdalena Chmur ◽  
Andrzej Bajguz
Keyword(s):  
Plant Growth ◽  
Growth And Development ◽  
Inhibitory Effect ◽  
Dependent Manner ◽  
Plant Growth And Development ◽  
Concentration Dependent Manner ◽  
Spectrophotometric Methods ◽  
Synthetic Inhibitor ◽  
Wolffia Arrhiza ◽  
First Time

Brassinolide (BL) represents brassinosteroids (BRs)—a group of phytohormones that are essential for plant growth and development. Brassinazole (Brz) is as a synthetic inhibitor of BRs’ biosynthesis. In the present study, the responses of Wolffia arrhiza to the treatment with BL, Brz, and the combination of BL with Brz were analyzed. The analysis of BRs and Brz was performed using LC-MS/MS. The photosynthetic pigments (chlorophylls, carotenes, and xanthophylls) levels were determined using HPLC, but protein and monosaccharides level using spectrophotometric methods. The obtained results indicated that BL and Brz influence W. arrhiza cultures in a concentration-dependent manner. The most stimulatory effects on the growth, level of BRs (BL, 24-epibrassinolide, 28-homobrassinolide, 28-norbrassinolide, catasterone, castasterone, 24-epicastasterone, typhasterol, and 6-deoxytyphasterol), and the content of pigments, protein, and monosaccharides, were observed in plants treated with 0.1 µM BL. Whereas the application of 1 µM and 10 µM Brz caused a significant decrease in duckweed weight and level of targeted compounds. Application of BL caused the mitigation of the Brz inhibitory effect and enhanced the BR level in duckweed treated with Brz. The level of BRs was reported for the first time in duckweed treated with BL and/or Brz.

scholarly journals Acclimation of cadmium-induced genotoxicity and oxidative stress in mung bean seedlings by priming effect of phytohormones and proline

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257924
Author(s):  
Meher Hassan ◽  
Muhammad Israr ◽  
Simeen Mansoor ◽  
Syeda Amna Hussain ◽  
Faiza Basheer ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Mitotic Index ◽  
Chromosomal Aberrations ◽  
Mung Bean ◽  
Growth Traits ◽  
Cadmium Stress ◽  
Dependent Manner ◽  
Root Tips ◽  
Maximum Decrease ◽  
Mutagenic Effects

In this research, eight local mung bean (Vigna radiata) varieties were analyzed for their performance against two levels of CdCl2 solution (0.3 and 0.5 mM) alone and priming with gibberellic acid (GA3) (100 μM), salicylic acid (SA) (50 μM) and proline (5 mM) solution prior to Cd exposure. Mung bean seedlings were analyzed for disturbance in cytological, morphological, biochemical and enzymatic parameters under cadmium stress. For cytological studies, 48 h grown mung bean seedlings root tips were used to prepare slides and studied for percent mitotic index (MI%) and to calculate percent C-mitosis, laggard, sticky and fragmented chromosomes, pictures were captured by a Nikon camera (DS-Fi 1 Japan) attached with a microscope. One-week grown mung seedlings were studied for growth traits, malondialdehyde (MDA), protein, proline and antioxidant enzymes. ANOVA and DMR test of this research revealed that all the tested mung bean varieties and treatments were significantly different regarding mitotic index and number of chromosomal aberrations. Both the Cd treatments exhibited increased total chromosomal aberrations with different types and a maximum decrease in MI%. In pretreated samples, GA3, SA and proline serve as mitigating agents that reduce mutagenic effects of Cd in mung bean by increasing MI% and decreasing chromosomal aberrations as compared to non-pretreated samples. Both the Cd treatments showed a decrease in all growth traits. Total proteins were also found to be significantly reduced in a dose-dependent manner in all genotypes. Cd treatment increased the activities of all antioxidant enzymes tested. Cd caused oxidative damage as indicated by elevated levels of MDA content in treated samples in comparison to control. Proline content levels were also high in Cd treated seedlings indicating stress. Results demonstrated that pretreatment with phytohormones and proline before Cd were found to improve all morphological parameters, by altering antioxidant enzymes activities along with a decrease in MDA and proline contents as well. It was further noticed that the performance of GA3 was better at 0.3 mM Cd treatment while SA was found to be a good mitigating agent at 0.5 mM Cd stress in all tested mung bean varieties. This research concluded less deleterious effects of Cd on AZRI-2006 while more sensitivity to NM-51 towards Cd. Priming with phytohormones and proline is a user-friendly, economical, and simple mitigation strategy to reduce Cd toxicity in plants and get better yield from contaminated lands.

scholarly journals Absorption Curve and Germination of Jucá Seeds under Different Salts

2016 ◽  
Vol 8 (7) ◽  
pp. 112
Author(s):  
Danielle Marie Macedo Sousa ◽  
Celsemy Eleutério Maia ◽  
Elis Regina Costa de Morais
Keyword(s):  
Electrical Conductivity ◽  
Salt Stress ◽  
Seed Germination ◽  
Water Absorption ◽  
Electric Conductivity ◽  
Growth And Development ◽  
Saline Solution ◽  
Distilled Water ◽  
Plant Tolerance ◽  
Water Control

<p>The plants are subjected to conditions of multiple stresses that limit their growth and development, which affects their chances of survival wherever they grow. To evaluate the plant tolerance to these conditions, one of the most used methods is the observation of seed germination under salt stress. The action of salts on the seeds varies widely among species and can exert toxic effects, causing damage before and/or after the onset of germination. Therefore, the objective of this work was to evaluate the influence of different salts in water absorption and germination in jucá seeds (<em>Libidibia ferrea</em>). For absorption and germination curve, were used non-scarified and scarified seeds, and subjected to the treatments in distilled water (control) and NaCl solutions, KCl, CaCl<sub>2</sub> and K<sub>2</sub>HPO<sub>4</sub>, in an electric conductivity of 5 dS m<sup>-1</sup>. The saline solution composed by K<sub>2</sub>HPO<sub>4</sub> influence on water absorption of <em>L. ferrea</em> in intact seeds. The scarified and non-scarified seeds of <em>L. ferrea</em> have different times for the physiological phases of water absorption. The action of salts in the germination and vigor in non-scarified and scarified seeds, does not significantly reduce these values due to electrical conductivity of 5 dS m<sup>-1</sup> not be enough to affect the seed germination of <em>L. ferrea</em>.</p>

scholarly journals Growth and development of Mung bean (Vigna radiata(L.)) depending on sowing methods

2020 ◽  
Vol 614 ◽  
pp. 012126
Author(s):  
N A Ravshanova ◽  
I Usmonov ◽  
A Chulliyev ◽  
B Isroilov ◽  
D Rahimova ◽  
...  
Keyword(s):  
Vigna Radiata ◽  
Mung Bean ◽  
Growth And Development

scholarly journals Increase in Endogenous and Exogenous Cyclic AMP Levels Inhibits Sclerotial Development in Sclerotinia sclerotiorum

1998 ◽  
Vol 64 (7) ◽  
pp. 2539-2544 ◽  
Author(s):  
Jeffrey A. Rollins ◽  
Martin B. Dickman
Keyword(s):  
Cyclic Amp ◽  
Sclerotinia Sclerotiorum ◽  
Growth And Development ◽  
Mycelial Growth ◽  
Dependent Manner ◽  
Wild Type ◽  
Concentration Dependent Manner ◽  
Sclerotial Development ◽  
Camp Levels ◽  
Methyl Xanthine

ABSTRACT Growth and development of a wild-type Sclerotinia sclerotiorum isolate were examined in the presence of various pharmacological compounds to investigate signal transduction pathways that influence the development of sclerotia. Compounds known to increase endogenous cyclic AMP (cAMP) levels in other organisms by inhibiting phosphodiesterase activity (caffeine and 3-isobutyl-1-methyl xanthine) or by activating adenylate cyclase (NaF) reduced or eliminated sclerotial development in S. sclerotiorum. Growth in the presence of 5 mM caffeine correlated with increased levels of endogenous cAMP in mycelia. In addition, incorporation of cAMP into the growth medium decreased or eliminated the production of sclerotia in a concentration-dependent manner and increased the accumulation of oxalic acid. Inhibition of sclerotial development was cAMP specific, as exogenous cyclic GMP, AMP, and ATP did not influence sclerotial development. Transfer of developing cultures to cAMP-containing medium at successive time points demonstrated that cAMP inhibits development prior to or during sclerotial initiation. Together, these results indicate that cAMP plays a role in the early transition between mycelial growth and sclerotial development.

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