Influence of priming with exogenous boron on the seed vigour of alfalfa (Medicago sativa L.)

Author(s):  
F. S. Xia ◽  
Y. C. Wang ◽  
H. S. Zhu ◽  
J. Y. Ma ◽  
Y. Y. Yang ◽  
...  

Alfalfa seeds were primed with 0, 0.1 %, 0.3 %, 0.6 %, 1.2 %, 2.4 % and 4.8 % (W/V) concentration of borax solution for 0 (control), 3, 6, 9 and 12 h at 20°C. The results showed that the vigour of alfalfa seeds were very significantly (P less than 0.01) related to boron concentration, priming time and their interaction, they were enhanced by the opportune concentration of boron priming, but were restrained by high concentration of boron. Priming with 0.1 % boron for 6 h was the best treatment for using to improve the seed vigour of alfalfa.


Author(s):  
F. S. Xia ◽  
Y. C. Wang ◽  
F. Wang ◽  
C. C. Wang ◽  
H. S. Zhu ◽  
...  

This experiment was designed to determine the influence of boron priming on seed vigour in thirteen varieties of alfalfa (Medicago sativa L.). Alfalfa seeds were primed with 1.8% (W/V) concentration of borax solution for 0, 3, 6, 9, 12 and 24 h at 20°C. The results showed that the original vigour of alfalfa seeds was disparate in thirteen varieties and there were highly significant (P less than 0.01) differences observed in both varieties and priming time and their interactions on the vigour of the seeds. Alfalfa seeds of WL525HQ and WL656HQ might be more sensitive to boron toxicity, but seeds of WL298HQ, WL343HQ, WL354HQ, WL903 and Pianguan were insensitive to boron toxicity. Thus, it is necessary to carefully select appropriate varieties of alfalfa seeds for the application of boron priming.



2014 ◽  
Vol 488-489 ◽  
pp. 1165-1168
Author(s):  
Xue Kun Zhang ◽  
Chun Hua Zhao

The compression properties of three varieties of alfalfa seeds, such as Medicago sativa L. cv. Gannong No.3, Medicago sativa L. Longdong and Medicago sativa L. cv. Golden Empress, are studied by extrusion experiments using SANS universal material testing machine. The results of Longdong alfalfa seed show that the fracture load has relation with shape and size of the alfalfa seeds. For both of ellipsoidal seed and arched seed, the fracture load of single seed is about 19N to 30N. The fracture load increases linearly with the thickness of plump seed with the ratio of thickness to width greater than 0.75. These inclusions for other two varieties are the same with Longdong alfalfa seed. This research could offer a technical basis of designing and developing of the alfalfa seeder and seed harvesting machine.



1979 ◽  
Vol 43 (7) ◽  
pp. 1499-1504 ◽  
Author(s):  
Takatoshi ITOH ◽  
Susumu SHIMURA ◽  
Susumu ADACHI


1994 ◽  
Vol 72 (8) ◽  
pp. 1121-1125 ◽  
Author(s):  
H. C. Huang ◽  
G. C. Kozub ◽  
E. G. Kokko

Hard seeds of alfalfa (Medicago sativa L.) were artificially inoculated with Verticillium albo-atrum Reinke & Berthold and assessed for survival of the pathogen under laboratory and field conditions. When infected seeds were stored air-dry, V. albo-atrum survival was low (less than 10% after 10 months) at above-freezing temperatures (20 and 30 °C) but high (more than 90% at 10 months) at subfreezing temperatures (−20 and −10 °C). Survival of the pathogen was also low (10% at 3 months) in seeds buried in soil at 20 °C but high (90% at 10 months) at −5 °C. More than 75% of the hard seeds remained viable after 18 months of air-dry storage at −20, −10, 4, 20, or 30 °C. The incidence of verticillium wilt in alfalfa plants due to seedborne V. albo-atrum decreased with seed storage period, averaging 40% after seed was stored for 1 month and zero when seed was stored for 12 or 18 months. In field experiments, survival of seedborne V. albo-atrum decreased with increasing period of burial but at different rates in each year. The percentage of seeds with viable V. albo-atrum was greater when the seeds were buried at 10 cm compared with 5 and 2 cm (48 vs. 39 and 38%, respectively). The complete loss of viability of V. albo-atrum in infected seeds stored at 30 °C for 6 months indicates that seed-borne V. albo-atrum could be eliminated by storing alfalfa seeds at 30 °C for 1 year. Key words: Verticillium wilt, Verticillium albo-atrum, Medicago sativa, alfalfa, survival, seedborne.



1963 ◽  
Vol 14 (5) ◽  
pp. 716 ◽  
Author(s):  
GB Jones ◽  
KO Godwin

Lucerne (Medicago sativa L.) in which the proteins were labelled with radioactive selenium was fed to mice. The distribution of 75Se among the various organs was studied. On the basis of 75Se activity per unit wet weight, the importance of the liver and kidney as metabolic sites is confirmed, and attention is directed to the pancreas, endocrines, and the stomach wall as additional sites where selenium is present in comparable concentrations. The relative importance of the above organs is supported by the results of autoradiography; in addition, this demonstrates the high concentration of 75Se in the cell nuclei, where selenium may play a fundamental role. Radioactive selenium from labelled lucerne was found in the milk of a lactating mouse within 4 hr of it being fed. Within 24 hr approximately 20% of the selenium absorbed by the dam had been transferred to the young mice through the milk.



1972 ◽  
Vol 14 (2) ◽  
pp. 221-226 ◽  
Author(s):  
K. Lesins

On crossing Medicago sativa L. with M. rhodopea Velen., two hybrids were obtained. One was a triploid 2n = 24. It was produced by crossing a self-incompatible, diploid (2n = 16) M. sativa plant with an artificially chromosome-doubled (2n = 32) M. rhodopea plant. In this hybrid almost no fully analyzable MI plates were found. The range of observed univalents in 57 MI plates examined was one to seven per plate; in 38 of these plates one to four trivalents were seen. The chromosomes were doubled in the triploid to produce a hexaploid hybrid (2n = 48) which was self-sterile. This was probably the influence of the self-incompatible parent. The amount of plasma-filled pollen was approximately 64% in the hexaploid hybrid, as compared to less than 20% in its triploid progenitor. On backcrossing the hexaploid hybrid to hexaploid and tetraploid alfalfa, seeds were readily obtained. The other hybrid, which was obtained by crossing a tetraploid (2n = 32), male-sterile M. sativa with the chromosome-doubled M. rhodopea; had 2n = 31. It is assumed that in this and similar rarely successful crosses, some incompatibility factor/s is eliminated with the eliminated chromosome or the genic ratio is changed favoring compatibility. Transfer of M. rhodopea genic material to cultivated alfalfa may be through crossing over at meiosis involving chromosomes of the two species. Such occurrences were indicated on examination of meiosis in the triploid hybrid. Another way to include M. rhodopea chromosome complements in alfalfa would be to produce hexaploid auto-alloploids consisting of two sets of M. rhodopea (Rp) and four sets of M. sativa (S) chromosomes.



Author(s):  
José F. REYES ◽  
Johannes P.F. De BRUIJN ◽  
Guillermo F. TOLOSA ◽  
Pedro M. AQUEVEQUE ◽  
Christian L. CORREA

Copper metal as a contact surface was studied during the germination of alfalfa seeds (Medicago sativa L.) inside a rotating drum on a laboratory scale and compared with a plastic surface of food-grade. A system of three rotating drums was used inside a thermo-regulated chamber to germinate seeds. Alfalfa seeds were inoculated with 4.2 log cfu g-1 of Escherichia coli and after 84 h of germination sprouts were evaluated for E. coli, mesophilic aerobic bacteria, the content of copper and other minerals (potassium, calcium, magnesium, sodium, iron, manganese, and zinc), total mass, unit mass and length, and color. The contact of alfalfa sprouts with copper sheets allowed to reduce the E. coli load from 6.54 to <0.1 log cfu g-1. However, all sprouts exceeded in copper (> 10 ppm) according to Food Sanitary Regulations. Germinated mass and length decreased after copper treatments. No statistically significant differences were observed between treatments for the remaining quality parameters. Finally, it is concluded that copper was very efficient in reducing the microbial load of E. coli in alfalfa sprouts, complying with the regulations established by the Chilean Ministry of Health.



Author(s):  
Francisco Gavi Reyes ◽  
César Botello-Aguillón ◽  
Leonardo Tijerina-Chávez ◽  
Arturo Galvis-Spíndola ◽  
Rodrigo Roblero-Hidalgo

E Objetivo: Desarrollar un procedimiento para estimar biomasa con imágenes digitales captadas desde un dron y modelación 3D (ID-Dron-3D) aplicable en alfalfa (Medicago sativa L.) y avena forrajera (Avena sativa L.). Diseño/metodología/aproximación: Con una cámara digital acoplada al dron se obtuvieron imágenes antes de la cosecha de los cultivos, que fueron procesadas con software para luego estimar volumen de biomasa. En cada cultivo se midió altura de la planta y área cosechada, volumen aparente y real de biomasa, y peso de biomasa fresca y seca. Resultados: Con base en el análisis de regresión se obtuvieron modelos lineales a una p<0.05 para predecir: biomasa fresca en avena (R2=0.70) y alfalfa (R2 =0.47); y biomasa seca en avena (R2=0.78) y en alfalfa (R2=0.31) mediante ID-Dron-3D. Limitaciones del estudio/implicaciones: Considerando las R2 de los modelos obtenidos, los resultados en la avena forrajera fueron mejores, respecto a los detectados en alfalfa, lo cual se puede deber a la mayor variabilidad de la cobertura vegetal, ya que, en algunas unidades de muestreo, las plantas de alfalfa no cubrían completamente el suelo. Hallazgos/conclusiones: El rendimiento de biomasa fresca y seca de ambos cultivos se correlacionó significativamente con su respectivo volumen aparente estimado con imágenes digitales tomadas desde un dron y su procesamiento 3D (ID-Dron-3D).



Author(s):  
CC. Castillo-Águilar

Se comparó el uso de diferentes dietas con base en heno de alfalfa cultivada (Medicago sativa L.) en Campeche y su relación con un concentrado comercial y pasto Taiwán (Pennisetum purpureum), T1=concentrado comercial, T2=T1+heno de alfalfa, T3=T1+pasto Taiwán, T4=heno de alfalfa. Se evaluó el comportamiento productivo y metabólico de corderos en sistema intensivo utilizando 20 corderos machos con encaste de Pelibuey, Dorper y Black Belly de 12.5±1 kg de peso vivo (PV). Se midió el consumo de materia seca total (CMS), la ganancia diaria de peso (GDP), la conversión alimenticia (CA), y la digestibilidad in situ de la materia seca (DISMS). También fueron evaluados el pH, nitrógeno amoniacal (NH3) y la concentración de ácidos grasos volátiles (AGV) en líquido ruminal. La mejor GDP en gramos por día, de 234 g (p?0.05) se obtuvo en el T2; en contraste, el T3 mostró la menor GDP. La dieta que incluyó la mezcla de heno de alfalfa y concentrado mejoró significativamente las condiciones de las variables pH, NH3 y AGV (p?0.05).



Author(s):  
Galina Stepanova

The article describes the main morphological and biological features of alfalfa varieties included in the State register of breeding achievements approved for use in the Central Chernozem zone of Russia. A total of 32 alfalfa varieties are included in the State register. This is 9 varieties of blue alfalfa (Medicago sativa L. subsp. Sativa) of domestic selection and 8 foreign, 11 varieties of variable alfalfa (Medicago sativa L. nothosubsp. varia (Martyn) Arcang.) domestic selection and 1 variety of foreign and 3 varieties of yellow alfalfa (Medicago sativa L. subsp. falcata (L.) Arcang.). It shows the average and maximum yield of varieties determined in the process of state variety testing, as well as independent evaluation in research institutions in the region. Varieties of blue alfalfa of domestic selection Kevsala, Elena, Satellite, Vavilovskaya Yubileynaya were the most productive. The average yield of dry matter of these varieties reaches 8.4–9.2 t/ha, the maximum — 15.3–17.7 t/ha. Alfalfa varieties Timbale and Galaxy were the most productive among foreign varieties: the average yield of dry matter was 8.1 and 8.3 t/ha, the maximum – 15.5 and 17.2 t/ha. Varieties of alfalfa variable Vitalina and Vela provided an average yield of dry matter of 7.6 and 9.0 t/ha, the maximum yield reached 15.4 and 18.1 t/ha.



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