Genetic variation and correlation studies between micronutrient (Fe and Zn), protein content and yield attributing traits in mungbean (Vigna. radiata L.)

Author(s):  
Renu Singh ◽  
Adriaan W. Van Heusden ◽  
Ram Kumar ◽  
Richard G.F. Visser

Mungbean can effectively contribute in alleviation of iron, zinc and protein malnutrition as it is a source of micronutrients and protein. To improve this cultivars have to be developed which are rich in micronutrients and protein. But in general more focus is given to quantitative traits such as yield. Breeding mungbean for enhanced grain nutrients is still in its start-up phase. The present study was carried out to access genetic variation for both quantitative as qualitative traits. The correlation between important traits such as yield and Fe, Zn, protein content was calculated. A positive correlation was found between iron and zinc content (r = 0.47) whereas no significant correlation with grain yield was observed indicating no compromise of yield for improving quality. Breeding a cultivar which is nutritionally improved along with high yield is therefore possible. A few promising cultivars with high micronutrients, protein and yield were identified. These cultivars can be used in specific breeding programs aiming at nutrient-rich high yielding cultivars.

Author(s):  
Avinash Kumar ◽  
Ashutosh Kumar ◽  
N. K. Singh ◽  
Rajesh Kumar ◽  
S. K. Singh ◽  
...  

In the present investigation, 10 parents and their 45 crosses (half diallel) along with 2 standard checks (Rajendra Nilam and Rajendra Mahsuri-1) were evaluated during Kharif, 2018 in Randomized Complete Block Design (RCBD) with 3 replications at Rice Farm Section, Dr. Rajendra Prasad Central Agricultural University (RPCAU), Pusa, Bihar. The objective of this study was to identify promising rice genotypes having desirable combination of morphological traits along with high grain iron and zinc content and high grain yield potential. The results of variability parameters indicated that ample amount of genetic variability was present for all the studied traits. Most of the traits showed high heritability coupled with high genetic advance indicating fruitfulness of selection for improvement of these traits. One genotype (P2×P7) with high grain iron (16.10 ppm) and grain zinc (26.40 ppm) content along with high yield (43.12 g/plant) was identified. Genotypes with high grain iron coupled with high grain yield (P7×P9, P8×P9, P5×P7 and P5×P9) and high  grain zinc content coupled with high grain yield (P4×P7, P9×P10, P8×P9 and P5×P7) were also identified. These promising genotypes identified can be used further in breeding programmes to obtain superior segregants with high grain micronutrient content and high grain yield.


2017 ◽  
Vol 6 (6) ◽  
pp. 45
Author(s):  
Nyabasi Makori ◽  
Neema Kassim ◽  
Joyce Kinabo ◽  
Athanasia Matemu

Evaluation of the quality of complementary flour and its nutritional adequacy in relation to infant’s nutritional requirements was done to four different types of complementary flour (composite cereals with groundnuts, maize, millet and sorghum). The collected samples were analysed for determination of macronutrient, micronutrients and phytate content. The mean protein content in all types of complementary flour was 7.30 ± 2.52 g/100 g. A significant difference in protein content between composite cereals (p ≤ 0.001) and other types of cereals-based foods was observed. Fat and iron content were found to be inadequate in all types of complementary flours. Phytate content ranged from 59.47 - 1176.8 mg/100 g, the highest content was observed in sorghum. On the other hand, the mean iron and zinc content in all types of complementary flour was 5.25 ± 1.35 mg/100 g and 2.99 ± 1.36 mg/100 g respectively. The molar ratios of phytate:iron and phytate:zinc were at a level that would tend to inhibit bioavailability of iron and zinc in 97% and 45% of analysed samples respectively. An association between anaemia and phytate:iron molar ratio (AOR = 4.2, 95%, 1.2 - 6.9) was observed. The quality and adequacy of nutrients in cereal-based complementary flour has shown to be inadequate and likely to be compromised by the presence of phytate. 


2012 ◽  
Vol 12 (4) ◽  
pp. 245-252 ◽  
Author(s):  
Jeane de Oliveira Moura ◽  
Maurisrael de Moura Rocha ◽  
Regina Lúcia Ferreira Gomes ◽  
Francisco Rodrigues Freire Filho ◽  
Kaesel Jackson Damasceno e Silva ◽  
...  

The objective of this study was to estimate the direct and indirect effects of agronomic and culinary traits on iron and zinc contents in 11 cowpea populations. Correlations between traits were estimated and decomposed into direct and indirect effects using path analysis. For the study populations, breeding for larger grain size, higher number of grains per pod, grain yield, reduced cooking time, and number of days to flowering can lead to decreases in the levels of iron and zinc in the grain. Genetic gains for the iron content can be obtained by direct selection for protein content by indirect effects on the number of grains per pod, 100-grain weight and grain yield. The positive direct effect of grain size and protein content on the zinc content indicates the possibility of simultaneous gain by combined selection of these traits.


Author(s):  
Ritu R. Saxena ◽  
Kanushree Nandedkar ◽  
Suman Rawte ◽  
S.S. Porte ◽  
Mary S. Xalxo ◽  
...  

Background: In an effort to develop micronutrient-rich chickpea lines, a study to examine the variability in yield and nutritional traits was conducted. Methods: 99 genotypes were studied, the data was recorded and analyzed on yield traits, protein, iron and zinc content in Rabi 2019-20. Result: Maximum variability was recorded in plant yield followed by iron concentration (mg/kg), hundred seed weight and number of pods per plant. Of the total entries, nine entries namely, RGH4, RGH56, RG2016-84, ICC251762, RGH53, IPC98-12, RG2016-03, ICC1053 and RGH58 recorded high protein content ( greater than 20%). All these entries possessing high protein had pink flower. One chickpea accession (RGH53) accumulated the highest concentration for both protein and zinc, with an average of 21.86% and 73.00 ppm, respectively, but it showed low iron concentration (20.77 ppm). The first six principal components provided a reasonable summary of the data and explained 80.19% of the total variation. Ninety nine genotypes were grouped into ten clusters. Maximum inter cluster distance was observed between clusters VI and IX; VI and VII and IV and VI. The genotypes of these clusters are suggested for utilization in the crossing programs to breed varieties of chickpea for high yield with amenability to nutrients.


2021 ◽  
Vol 58 (3) ◽  
pp. 394-408
Author(s):  
Jeena George ◽  
VG Jayalekshmy ◽  
AM Shahiba ◽  
Arun Chacko ◽  
Tejashree S Lachyan

Kerala is rich with traditional rice varieties that could grow in various agro- climatic conditions of the state. However many of these traditional varieties are not cultivated and have become extinct due to low yield, promotion of high breed variety seeds and high-cost in continuing the cultivation with traditional seeds. The lack of knowledge about the nutraceutical values of traditional rice varieties among farmers leads to the non-participation in cultivation. Present study was aimed at to characterization of thirty traditional rice varieties in terms of physico-chemical, cooking quality as well as molecular screening with markers specific to quality traits. All the varieties used is unique in their quality parameters, among them Njavara Veluthath (13.57%) and Orumundakan (13.35%) were rich in protein content whereas, Karimbalan (311.54 mg/Kg and 102.41 mg/kg) and Veliyan (218.25 mg/Kg and 56.88mg/kg) were superior in Iron and Zinc content. The carotene content ranged from 0.14 mg/100g (Mullan kayama) to 0.56 mg/100g (Njavara veluthath) and amylose content was maximum in Cheruviripp (29.52%). Thus, these varieties need to be conserved and promote them by commercialize and through general public awareness about their medicinal benefits. Correlation analysis showed positive significant relationship between Iron and Zinc content, while amylose content had positive correlation with volume expansion and optimum cooking quality. The SSR marker RM535 linked to Quantitative Trait Loci for Iron and Zinc content was detected in thirteen varieties, RM190 linked with Wx gene locus associated with amylose content in seventeen varieties and the marker RM520 linked to QTL for protein content was detected in all the varieties.


2000 ◽  
Vol 53 ◽  
pp. 97-102 ◽  
Author(s):  
G. Ye ◽  
D.L. McNeil ◽  
G.D. Hill

This paper summarises existing studies of the genetics of resistance of lentils to Ascochyta blight and the genetic variation among pathogen populations with particular emphasis on the results from our programme Breeding methods are discussed Six pathotypes have been identified Resistance is mainly under the control of major genes but minor genes also play a role Current breeding programs are based on crossing resistant cultivars with high yield cultivars and multilocation testing Gene pyramiding exploring slow blighting and partial resistance and the use of genes from wild relatives will be the methods used in future


2021 ◽  
Author(s):  
C. Tara Satyavathi ◽  
Rukam S. Tomar ◽  
Supriya Ambawat ◽  
Jasminkumar Kheni ◽  
Shital M. Padhiyar ◽  
...  

Abstract Pearl millet is an important staple food crop of poor people which is rich in micronutrients like iron and zinc and amenable for focused breeding for these micronutrients along with high yield. Transcriptome sequencing using ION S5 Next Generation Sequencer generated 43.5 million sequence reads resulting in 83,721 transcripts with N50 of 597 bp and 84.35% of transcripts matched with the pearl millet genome assembly. The genotypes having high Fe and Zn showed differential gene expression during different stages. Of which, 155 were up-regulated and 251were down-regulated while during flowering stage and milking stage 349 and 378 transcripts were differentially expressed, respectively. Gene annotation and GO term showed the presence of transcripts involved in metabolic activities associated with uptake and transport of iron and zinc. In the present study, the 83,721 transcripts were also examined for identification of SSRs. A total of 4,327 SSRs were identified with dominance of tri-nucleotide SSRs in comparison to di-nucleotide SSRs. These EST-SSRs can be used in molecular breeding, genetic diversity analysis and determination of heterozygosity of the allelic loci. Information generated will help in gaining insights into iron and zinc metabolism and develop genotypes with high yield, grain iron and zinc content.


2021 ◽  
Vol 2 ◽  
Author(s):  
Mohammad Sohidul Islam ◽  
Md. K. Hasan ◽  
Bushra Islam ◽  
Nowrin Akhter Renu ◽  
Mohammad A. Hakim ◽  
...  

This study evaluated the role of gibberellic acid [GA3; (0, 100, 200, and 300 ppm)] in modulation of the growth, physiology, yield, and quality traits in two varieties (BARI Mung-6 and BARI Mung-8) of mungbean (Vigna radiata L.). Irrespective of the two varieties (BARI Mung-6 and BARI Mung-8), 100, 200, and 300 ppm of GA3 differentially modulated the tested parameters (relative water content, RWC; photosynthetic pigments: chlorophyll a, chlorophyll b, and carotenoids; growth parameters: fresh and dry weights of leaves, petioles, stems, and roots; yield contributing traits such as plant height, number of pods plant−1, number of grains pod−1, pod length, and 100-grain weight; quality traits such as grain nitrogen and protein). However, compared to the lowest GA3 (100 ppm) and the highest GA3 (300 ppm), the moderate concentration of GA3 (200 ppm) led to highest values of leaf-RWC, where this parameter exhibited 16.1 and 13.4% increase in BARI Mung-8 and BARI Mung-6, respectively. Similarly, the tested herein growth parameters and the yield traits significantly increased up to the foliar application of the moderate GA3 concentration (200 ppm), and thereafter these traits decreased with 300 ppm GA3. The 200 ppm-led changes in the growth and yield traits were significantly higher in BARI Mung-8 when compared to BARI Mung-6. Considering the quality traits, GA3 positively influenced the nitrogen and protein content in grains, where 200 ppm of GA3 led to increases of 25.2% in N, and 17.7% in protein over control in BARI Mung-6; whereas, BARI Mung-8 exhibited 28.3% in N, and 18.3% in protein with 200 ppm GA3 over control. Overall, BARI Mung-8 significantly responded to the foliar supply of 200 ppm GA3 when compared to BARI Mung-6. Hence, in order to high yield and grain protein content, the application of 200 ppm GA3 may be applied in V. radiata before and during flowering. The major mechanisms underlying the responses of the water relation, growth, and yield traits to the GA3 concentrations need to be explored.


2020 ◽  
Vol 17 (1) ◽  
pp. 48-58
Author(s):  
Cristina Z. de Morais C. Dias-Barbosa ◽  
Diego S.V. de Oliveira ◽  
Kaesel J.D. de Oliveira ◽  
Regilda S. dos Reis Moreira Araújo ◽  
Maurisrael de Moura Rocha

Background: Cowpea is a very popular crop in Brazil, rich in nutrients that can be used as food to feed the population, avoiding deficiencies caused by the lack of minerals such as iron and zinc. Objective: To select elite cowpea lines for biofortification of iron and zinc and determine the physical and chemical characteristics of the lines. We analyzed: 33 cowpea genotypes, being 31 elite genotypes and two biofortified cultivars (control). Method: The 10 best lines with iron and zinc contents were selected and, together with the controls, were analyzed for chemical composition (moisture, ash, lipids, proteins and carbohydrates), total energy value and cooking time. Results: The iron and zinc contents in semi-prostrate genotypes were higher than those in semi-erect genotypes, all the semi-prostrate lines presented a high iron content, and 19.25% presented a high zinc content. The genotypes of the semi-erect lines exhibited more significant genetic variability for iron content, whereas those of the semi-prostrate genotypes showed more significant genetic variation for zinc content, both exhibited a high genetic component in phenotypic expression. Improving the genotypes for increased protein content led to reductions in lipid and carbohydrate contents, as well as the total energy value, whereas increasing the carbohydrate content increased both the total energy value and the zinc content. The MNC04-792F-146 lines presented alleles that favored an increase in carbohydrate content and total energy, while the MNC04-769F-26, MNC04-769F-31, and MNC04-774F-90 lines were shown to be good sources of genes for increasing protein content, decreasing lipid content, and rapid cooking. Conclusion: The MNC04-762F-9, MNC04-792F-146, and MNC04-769F-55 lines exhibited the greatest potential to be released as iron and zinc biofortified cultivars.


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