scholarly journals AKTIVITAS ANTIBAKTERI JAMU MADURA SUBUR KANDUNGAN

el–Hayah ◽  
2015 ◽  
Vol 5 (2) ◽  
pp. 65
Author(s):  
Andik Wijayanto ◽  
Romi Abrori
Keyword(s):  
Centella Asiatica ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Antibacterial Drug ◽  
E Coli ◽  
Kaempferia Galanga ◽  
Test Organisms ◽  
And Staphylococcus Aureus ◽  
Paper Disc

<p><em>Madurese traditional </em><em>medicine</em><em> (herbs) Subur Kandungan is </em><em>a medicine</em><em> that is widely used</em><em> and produced</em><em> in Madura that contains Curcuma zedoaria</em><em> </em>(Chrism.)Rosc<em>., Kaempferia galanga </em>L<em>., </em><em>Foeniculum</em><em> </em><em>vulgare </em>Mill.<em>, and </em><em>Centella asiatica </em>(L.)Urb.<em> </em><em>highly favoredby the Indonesian community</em><em>, especially women to improve reproductive health but have not been tested scientifically and standardization to ensure efficacy and safety. Initial stageis screening bioactive compound and then antibacterial activity assay with paper disc method. </em><em>Clinical bacterial isolates such as Escherichia coli and Staphylococcus aureus were used as test organisms. </em><em>The best treatment to inhibit the growth of </em><em>E. Coli </em><em>and </em><em>S. aureus</em><em>is 70</em><em>% ethanol at a concentration of extract 1000 mg/ml with inhibition zone respectively 5mm and 6.5mm. Although in crude extract form, </em><em>Madurese traditional </em><em>medicine</em><em> (herbs) Subur Kandungan </em><em>had inhibitory effect on the growth of the E.coli and S.aureus so this </em><em>traditional </em><em>medicine</em><em> </em><em>potential as an antibacterial agent</em><em>. </em><em>It is recommended that further work be done to identify the specific secondary metabolite compound that responsible for this effect, purify it, and standardize as same as a antibacterial drug.</em><em></em></p>

scholarly journals Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Megawati S. Saroinsong ◽  
Febby E. F. Kandou ◽  
Adelfia Papu ◽  
Marina F. O. Singkoh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Activity Test ◽  
And Staphylococcus Aureus ◽  
Paper Disc ◽  
Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

scholarly journals Antibacterial activity of Escherichia coli from squid ink (Loligo sp.) n-Hexane extracts

2021 ◽  
Vol 869 (1) ◽  
pp. 012033
Author(s):  
D R Utami ◽  
I Irwan ◽  
S Agustina ◽  
S Karina ◽  
S Afriani
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Bioactive Compound ◽  
Inhibition Zone ◽  
Hexane Extract ◽  
Inhibition Zone Diameter ◽  
E Coli ◽  
Hexane Extracts ◽  
Intermediate Concentration

Abstract Squid is one of the export commodities in Indonesia. In general, the use of squid meat, while the ink is only as waste. In fact, Squid ink contain bioactive compound that potential as anti-inflammatory, antihypertensive, anti-diabetic,anti-microbial and anti-malaria agents. The purpose of the study is to determine the types of secondary metabolite compounds contained in n-hexane extract of Loligo sp. ink using maceration method to determine its antibacterial activity against Escherichia coli. The results of secondary metabolite compounds obtained from the n-hexane extract of Loligo sp. ink are alkaloid, saponins, glycosides and phenol. The results of antibacterial test against E. coli using the disc method obtained the average of inhibition zone diameter at the concentration of 4% is 6.3 mm (intermediate), concentration of 8% is 7.83 mm (intermediate), concentration of 16% is 14.5 mm (susceptible) and concentration of 32% is 10.83 mm (intermediate). The antibacterial activity in n-hexane extract of Loligo sp. ink is optimal at the concentration of 16% against E. coli bacteria.

Note: Inhibition of Pathogenic Bacteria by Essential Oils at Different Concentrations

2003 ◽  
Vol 9 (2) ◽  
pp. 85-88 ◽  
Author(s):  
G. Özkan ◽  
O. Sağdiç ◽  
M. Özcan
Keyword(s):  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Agents ◽  
Antimicrobial Effect ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Paper Disc

The antimicrobial effect of 11 selected Turkish spice essential oils was investigated against seventeen pathogenic bacteria. The antimicrobial activity of the essential oils of six spices (cumin, fennel, laurel, mint, marjoram, oregano, pickling herb, sage, savory, thyme (black) and thyme) was tested at four concentrations (0.2, 0.4, 1 and 2%) on various microorganisms ( E. aerogenes, E. coli, E. coli O157:H7, K. pneumoniae, P. vulgaris, S. enteritidis, S. gallinarum, S. typhimurium, S. aureus, Y. enterocolitica, A. hydrophila, C. xerosis, M. luteus, M. smegmatis, E. feacalis, P. aeruginosa and P. fluorescens). All preparations showed antibacterial activity against at least one or more bacteria. The inhibitory effect of the essential oils was evaluated through paper disc diffusion method. In general, the essential oils at 1 and 2% levels were effective. The most active essential oils were marjoram, thyme and oregano. According to the results, the studied essential oils potentially might be used as antibacterial agents to prevent the spoilage of food products, although further research is needed.

scholarly journals Isolation and Antibacterial Activity of Endophytic Bacteria Isolates from Gambir (Uncaria gambir (Hunter) Roxb.)

2019 ◽  
Vol 12 (6) ◽  
pp. 139-146
Author(s):  
Suryani Ompusunggu ◽  
Dwi Suryanto ◽  
Yurnaliza
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
And Staphylococcus Aureus ◽  
Paper Disc

A study on isolation and antibacterial activity of endophytic bacteria of gambir (Uncaria gambir (Hunter) Roxb.), along with its antibacterial extract activity test on clinical pathogenic bacteria has been conducted. Isolation of gambir endophytic bacteria was done in nutrient agar incubated at ambient temperature for 48 hours. Samples of gambir leaves and root was used for source of endophytic bacteria. Bacterial isolate was propagated using Mueller Hinton Agar. Bacterial culture was extracted using methanol as solvent. To test methanol extract activity of bacterial isolates, concentration of 20, 40, 60, 80, and 100% (v/v) were used in paper disc. Pathogenic bacterial isolates such as Escherichia coli, Streptococcus mutans, and Staphylococcus aureus were used for antibacterial test. Inhibition zone formed around paper disc indicated antibacterial extract activity against the bacteria. Endophytic bacterial isolates of gambirwas identified using Vitek Compact 2.0® based on biochemical test. Isolates SO02 and SO03 showed relatively higher antibacterial activity, with inhibition zone of 17 and 30 mm, respectively. Methanol extract of SO02 showed to have higher activity of >10 mm at 100% of extract concentration, compared to that of SO03 which only showed <10 mm) against E. coli.High inhibition against S.mutans and S.aureus was obtained at of 60%. Chemical identification found that methanol extract of SO02 and SO03 showed to have flavonoid and tanning group. Bacterial identification showed that SO02 and SO03 were closely related to Enterobacter cloacae and Gemella morbillorum with similarity of 98 and 86%, respectively. However, accurate species of these two isolates should be determined

scholarly journals THE EFFECT OF ALCOHOLIC EXTRACT Eriobotrya japonica LEAVES ON SOME BACTERIAL GENES AND SOME CHEMICAL AND MICROBIOLOGICAL PROPERTIES OF BEEF

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Alwash & et al.
Keyword(s):  
Bacterial Strain ◽  
Thiobarbituric Acid ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Eriobotrya Japonica ◽  
Alcoholic Extract ◽  
E Coli ◽  
Cow Meat ◽  
Bacterial Genes ◽  
Microbiological Properties

This study was conducted to investigate the effect of using alcoholic extract of yangadnia leaves on some bacterial strain Salmonella typhimiurum, E.coli  and  staphylococcus aures  in 200, 100, 50 and 25 mg/ml concentration using digging method. Minced cow meat 200 mg/ml   was mixed with yangdina extract and stored for 0,2,4 day at 4 C and tested for chemical and biological characteristics. The ruselts showed that the best concetration of inhibition was 200mg/ml and the inhibition zone diameters were 26, 19, 15 mm for S. aures, S. typhimiurm    and E .coli  respectively. In respect to Effect of extract in minced cow the ruselts showed drop in  values of, thiobarbituric acid (TBA),total violate nitrogen (TVN), total number account of bactria and count of psycrotrophic bactria and effect on PH values. It can be concluded that  yangdina leaves extract has inhibitory effect on bacterial strain and best concetration  200mg/ml. Mixing  meat with extract improved the storage life at 4c for 4 day. 

scholarly journals The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 3 (1) ◽  
pp. 56-63
Author(s):  
Julia Nanda Puspita ◽  
Rikhsan Kurniatuhadi ◽  
Rahmawati Rahmawati
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
And Staphylococcus Aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).

scholarly journals THE EFFECT OF ALCOHOLIC EXTRACT Eriobotrya japonica LEAVES ON SOME BACTERIAL GENES AND SOME CHEMICAL AND MICROBIOLOGICAL PROPERTIES OF BEEF

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Alwash & et al.
Keyword(s):  
Bacterial Strain ◽  
Thiobarbituric Acid ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Eriobotrya Japonica ◽  
Alcoholic Extract ◽  
E Coli ◽  
Cow Meat ◽  
Bacterial Genes ◽  
Microbiological Properties

This study was conducted to investigate the effect of using alcoholic extract of yangadnia leaves on some bacterial strain Salmonella typhimiurum, E.coli  and  staphylococcus aures  in 200, 100, 50 and 25 mg/ml concentration using digging method. Minced cow meat 200 mg/ml   was mixed with yangdina extract and stored for 0,2,4 day at 4 C and tested for chemical and biological characteristics. The ruselts showed that the best concetration of inhibition was 200mg/ml and the inhibition zone diameters were 26, 19, 15 mm for S. aures, S. typhimiurm    and E .coli  respectively. In respect to Effect of extract in minced cow the ruselts showed drop in  values of, thiobarbituric acid (TBA),total violate nitrogen (TVN), total number account of bactria and count of psycrotrophic bactria and effect on PH values. It can be concluded that  yangdina leaves extract has inhibitory effect on bacterial strain and best concetration  200mg/ml. Mixing  meat with extract improved the storage life at 4c for 4 day. 

Inhibition of Pathogenic Bacteria by Camel's Milk: Relation to Whey Lysozyme and Stage of Lactation

1984 ◽  
Vol 47 (11) ◽  
pp. 838-840 ◽  
Author(s):  
ELIE K. BARBOUR ◽  
NASSIM H. NABBUT ◽  
WAYNE M. FRERICHS ◽  
HABEEB M. AL-NAKHLI
Keyword(s):  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Shigella Dysenteriae ◽  
Milk Samples ◽  
Stage Of Lactation ◽  
California Mastitis Test ◽  
Two Samples ◽  
Test Organisms ◽  
Camel's Milk

Twenty (10%) of 200 milk samples collected from different individual camels inhibited growth of one or more of six pathogenic test organisms in a filter-paper disk assay. The milk samples with inhibitory properties scored zero in the California Mastitis Test. The percentages of milk samples which inhibited Clostridium perfringens, Staphylococcus aureus, Shigella dysenteriae and Salmonella typhimurium were 7.5, 4.0, 2.0 and 1%, respectively. None of the 200 samples inhibited Bacillus cereus or Escherichia coli. Five milk samples inhibited growth of two test organisms and two samples inhibited growth of three organisms. Inhibition zone diameters ranged from 13.5 mm to 22.0 mm (average of 15 mm) using a disk of 12.5 mm in diameter. The average amount of lysozyme in the whey of 58 samples of camel's milk was 288 μg/100 ml. The average lysozyme content of the 20 samples showing growth inhibition of one or more test organisms was 648 μg/100 ml, significantly higher (P&lt;0.01) than the average in the 38 samples (62.6 μg/100 ml) that had no inhibitory effect. There was a negative correlation (r= −0.669) between lysozyme content in the whey of milk samples (y) and the stage of lactation of the camels (x) with the following regression equation: y = −2.86x + 665.8.

scholarly journals Potential Application of Murraya koenigii Extract as Antibacterial Agent in Liquid Hand Soap Formulation

2021 ◽  
Vol 6 (2) ◽  
pp. 97-100
Author(s):  
Ai Yin Loh ◽  
Khomaizon A.K. Pahirulzaman
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Agent ◽  
Antibacterial Agents ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Murraya Koenigii ◽  
Disc Diffusion Method ◽  
E Coli ◽  
And Staphylococcus Aureus ◽  
Paper Disc

Here we present the efficacies of Murraya koenigii leaves extract as natural antibacterial agents bythe ability to inhibit the growth of Escherichia coli and Staphylococcus aureus. The antibacterialactivities of formulated liquid hand soap with different concentrations (10, 30 and 50 mg/mL) ofM. koenigii extracts were determined by paper disc diffusion method. M. koenigii leaves extract at10 mg/mL concentration had the ability to inhibit the growth of both test microorganisms. Whereas,for liquid hand soap formulated with 10, 30 and 50 mg/mL M. koenigii extracts, inhibition zoneswere observed on S. aureus assay plates but not on E. coli. Hence, the results suggested that liquidhand soap containing M. koenigii extract had therapeutic potentials to prevent spread of skindiseases caused by S. aureus.

scholarly journals Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus

2019 ◽  
Vol 7 (22) ◽  
pp. 3882-3886
Author(s):  
Yessi Febriani ◽  
Vriezka Mierza ◽  
Novi Putri Handayani ◽  
Surismayanti Surismayanti ◽  
Ibrenaita Ginting
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Chromatography Method ◽  
E Coli ◽  
Layer Chromatography ◽  
And Staphylococcus Aureus

BACKGROUND: Infection is the most common diseases in developing country, including Indonesia. Bacteria that often causes infection is Escherichia coli and Staphylococcus aureus. One of the traditional plants that can be used as an antibacterial is lidah mertua. AIM: The purpose of this study was to find out the profile of chemical compounds by thin layer chromatography method and determine the antibacterial activity of Lidah Mertua leaves by in vitro. METHODS: This research conducted an experimentally using non-polar, semipolar, and polar as solvents to get extract against E. coli and S. aureus as bacterial testing. The antibacterial activity using agar diffusion method to get minimum inhibitory concentration (MIC). RESULTS: The result of the research on thin layer chromatography showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The data obtained were tabulated and analysed descriptively. The antibacterial activity show that n-hexane extract does not provide inhibitory activity. MIC value show that aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 50 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.50 mm and 8.20 mm and methanol extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 12.5 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.46 mm and 8.32 mm. CONCLUSION: The profile of chemical compounds by thin layer chromatography method showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The antibacterial activity show that n-hexane extract does not provide inhibitory activity, but aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus.

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