In vitro antifungal activity of Pongamia pinnata against collar rot pathogen (Sclerotium rolfsii) of Chickpea

Aims: This study is aimed to evaluate the in vitro antifungal activity effect of the crude aqueous extract (CAE), hydrolate (HY) and essential oil (EO) of Corymbia citriodora, Cymbopogon citratus, Cymbopogon flexuosus and Curcuma longa against the phytopathogenic fungi Alternaria steviae, Botryosphaeria dothidea, Colletotrichum gloeosporioides and Sclerotium rolfsii, and assess, in situ, the effectiveness of CAE of medicinal plants in reducing the severity of the cucumber anthracnose. Methodology: The EOs and HYs were obtained by hydrodistillation. The CAEs were prepared by the turbolysis method. Mycelial growth of the fungi was measured daily, by the diametrically opposite method. In the in vivo test, the CAEs were sprayed on the cotyledon leaves of healthy cucumber plants with three days after were inoculated with C. lagenarium. The severity of assessment of the disease was based on a scale of notes. Results: The medicinal plants studied showed antifungal activity against all or almost all pathogens. In general, treatment with CAE and HY of C. longa revealed the highest inhibition against the fungi tested. With the exception of the EO of C. longa, the other EOs showed total inhibition against all the fungi and in all the concentrations tested. Compared to control, in in vivo assays CAE of C. citratus presents a potential for control of cucumber anthracnose reducing the severity of the disease. Conclusion: The medicinal plants studied produce compounds associated with antimicrobial activity.

Download Full-text

In vitro antifungal activity of three Saudi plant extracts against some Phytopathogenic fungi

Journal of Plant Protection Research ◽

10.2478/v10045-012-0073-9 ◽

2012 ◽

Vol 52 (4) ◽

pp. 458-462 ◽

Cited By ~ 12

Author(s):

Abdul Aziz A. Al-Askar

Keyword(s):

Antifungal Activity ◽

Plant Extracts ◽

Sclerotium Rolfsii ◽

Ethanolic Extract ◽

Punica Granatum ◽

Phytopathogenic Fungi ◽

Plant Diseases ◽

High Concentration ◽

In Vitro Antifungal Activity

Abstract The antifungal activities of ethanolic extracts of three Saudi plants; camel thorn (Alhagi maurorum Medic.), caper (Capparisspinosa L.), and pomegranate (Punica granatum L.) were investigated in vitro against Alternaria alternata, Fusarium oxysporum, Phomadestructiva, Rhizoctonia solani, and Sclerotium rolfsii at concentrations of 0, 3, 6, and 9% (v/v). All tested plant extracts; seeds, roots, and rinds had different degrees of antifungal activity against the tested fungi. When compared with the control, the highest antifungal activity was recorded for camel thorn seeds extract at a concentration of 9%, while, pomegranate rinds extract at 9% came in second. Camel thorn rinds extract came in last even when used at a high concentration. The ethanolic extract of camel thorn seeds may be recommended as a potent bio-fungicide. Extensive studies should be undertaken for the ethanolic extract of camel thorn seeds as a strong antifungal agent against fungal plant diseases.

Download Full-text

In vitro antifungal activity and GC/MS profiling of ten selected Sudanese essential oils against Madurella mycetomatis, the causative agent of black-grain mycetoma

Planta Medica ◽

10.1055/s-0035-1565722 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

SA Khalid ◽

K Veres ◽

J Hohmann ◽

ME Elamin ◽

S Abd Algaffar

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Causative Agent ◽

Madurella Mycetomatis ◽

In Vitro Antifungal Activity

Download Full-text

In vitro Antifungal Activity of a 29-kDa Glycoprotein Purified from the Galls of Quercus infectoria

Acta Phytopathologica et Entomologica Hungarica ◽

10.1556/aphyt.40.2005.1-2.6 ◽

2005 ◽

Vol 40 (1-2) ◽

pp. 43-54 ◽

Cited By ~ 9

Author(s):

K. Yamunarani ◽

R. Jaganathan ◽

R. Bhaskaran ◽

P. Govindaraju ◽

R. Velazhahan

Keyword(s):

Antifungal Activity ◽

Quercus Infectoria ◽

In Vitro Antifungal Activity

Download Full-text

Formulation and Evaluation of Carbopol Gel Containing Liposomes of Ketoconazole. (Part-II)

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v1i2.8839 ◽

2009 ◽

Vol 1 (2) ◽

Cited By ~ 1

Author(s):

Rakesh Patel ◽

Hardik Patel ◽

Ashok Baria

Keyword(s):

Antifungal Activity ◽

Rat Skin ◽

Albino Rat ◽

In Vitro Drug Release ◽

Carbopol Gel ◽

Liposomal Formulations ◽

Release Drug ◽

In Vitro Permeation ◽

In Vitro Antifungal Activity

The aim of this work was to prepare and evaluate the topical carbopol gel formulation containing ketoconazole encapsulated liposomes. Ketoconazole loaded liposomes were prepared by thin film hydration technique. The prepared liposomes were incorporated into 1% carbopol gel, and the systems were evaluated for in-vitro drug release, drug retention into skin and in-vitro antifungal activity. The in-vitro permeation of ketoconazole using wistar albino rat skin from liposomal gel was compared with that of plain drug gel and also with plain drug cream containing 2% w/w of ketoconazole. The release of ketoconazole from liposomal gel was much slower than from non liposomal formulations. Gel containing liposomal ketoconazole showed maximum antifungal activity after 30 hours over plain ketoconazole gel and cream formulations.

Download Full-text

Management of collar rot disease in chickpea by Trichoderma species

Journal of AgriSearch ◽

10.21921/jas.v7i03.18694 ◽

2020 ◽

Vol 7 (03) ◽

Author(s):

PREM PANDEY ◽

G. C. SAGAR ◽

SUNDARMAN SHRESTHA2 ◽

HIRAKAJI MANANDHAR ◽

RITESH K. YADAV ◽

...

Keyword(s):

Growth Inhibition ◽

Mycelial Growth ◽

Sclerotium Rolfsii ◽

Maximum Growth ◽

Pot Experiment ◽

Trichoderma Spp ◽

Trichoderma Species ◽

Collar Rot ◽

Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

Download Full-text

Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro

Pharmaceutics ◽

10.3390/pharmaceutics13070998 ◽

2021 ◽

Vol 13 (7) ◽

pp. 998

Author(s):

Virgínia Barreto Lordello ◽

Andréia Bagliotti Meneguin ◽

Sarah Raquel de Annunzio ◽

Maria Pía Taranto ◽

Marlus Chorilli ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Enterococcus Faecium ◽

Potato Starch ◽

Oral Candidiasis ◽

Candida Spp ◽

Sem Images ◽

Control Film ◽

In Vitro Antifungal Activity

Background: Probiotic bacteria have been emerging as a trustworthy choice for the prevention and treatment of Candida spp. infections. This study aimed to develop and characterize an orodispersible film (ODF) for delivering the potentially probiotic Enterococcus faecium CRL 183 into the oral cavity, evaluating its in vitro antifungal activity against Candida albicans. Methods and Results: The ODF was composed by carboxymethylcellulose, gelatin, and potato starch, and its physical, chemical, and mechanical properties were studied. The probiotic resistance and viability during processing and storage were evaluated as well as its in vitro antifungal activity against C. albicans. The ODFs were thin, resistant, and flexible, with neutral pH and microbiologically safe. The probiotic resisted the ODF obtaining process, demonstrating high viability (>9 log10 CFU·g−1), up to 90 days of storage at room temperature. The Probiotic Film promoted 68.9% of reduction in fungal early biofilm and 91.2% in its mature biofilm compared to the group stimulated with the control film. Those results were confirmed through SEM images. Conclusion: The probiotic ODF developed is a promising strategy to prevent oral candidiasis, since it permits the local probiotic delivery, which in turn was able to reduce C. albicans biofilm formation.

Download Full-text

Rhizosphere-enriched microbes as a pool to design synthetic communities for reproducible beneficial outputs

FEMS Microbiology Ecology ◽

10.1093/femsec/fiz138 ◽

2019 ◽

Cited By ~ 6

Author(s):

Maria-Dimitra Tsolakidou ◽

Ioannis A Stringlis ◽

Natalia Fanega-Sleziak ◽

Stella Papageorgiou ◽

Antria Tsalakou ◽

...

Keyword(s):

Antifungal Activity ◽

Fungal Pathogens ◽

Growth Parameters ◽

Tomato Plants ◽

Beneficial Effects ◽

Negative Effect ◽

Tomato Growth ◽

In Vitro Antifungal Activity

Abstract Composts represent a sustainable way to suppress diseases and improve plant growth. Identification of compost-derived microbial communities enriched in the rhizosphere of plants and characterization of their traits, could facilitate the design of microbial synthetic communities (SynComs) that upon soil inoculation could yield consistent beneficial effects towards plants. Here, we characterized a collection of compost-derived bacteria, previously isolated from tomato rhizosphere, for in vitro antifungal activity against soil-borne fungal pathogens and for their potential to change growth parameters in Arabidopsis. We further assessed root-competitive traits in the dominant rhizospheric genus Bacillus. Certain isolated rhizobacteria displayed antifungal activity against the tested pathogens and affected growth of Arabidopsis, and Bacilli members possessed several enzymatic activities. Subsequently, we designed two SynComs with different composition and tested their effect on Arabidopsis and tomato growth and health. SynCom1, consisting of different bacterial genera, displayed negative effect on Arabidopsis in vitro, but promoted tomato growth in pots. SynCom2, consisting of Bacilli, didn't affect Arabidopsis growth, enhanced tomato growth and suppressed Fusarium wilt symptoms. Overall, we found selection of compost-derived microbes with beneficial properties in the rhizosphere of tomato plants, and observed that application of SynComs on poor substrates can yield reproducible plant phenotypes.

Download Full-text