scholarly journals Evaluación química y emisión de gas In-vitro en ensilados mixtos de King Grass y Clitoria-ternatea

2022 ◽  
Vol 8 (II) ◽  
Author(s):  
Serafín Jacobo López-Garrido ◽  
Diego Arturo Ramos-Ramos ◽  
Mónica Marcela Galicia-Jiménez ◽  
Marco Antonio Camacho-Escobar ◽  
Narciso Ysac Ávila-Serrano ◽  
...  
Keyword(s):  

El estudio evaluó la composición química, degradabilidad de la materia seca, producción de metano (CH4) y dióxido de carbono (CO2) en ensilados mixtos. Se realizaron micro silos de King grass CT-115 con la inclusión de 20, 30 y 40% de Clitoria ternatea adicionando de 5.0% de melaza y 0.5% de ácido fosfórico. Después de 40 días de fermentación, se determinó materia seca (MS), proteína cruda (PC), fibra detergente neutro (FDN), fibra detergente ácido (FDA), pH, nitrógeno amoniacal (NA) y ácidos grasos volátiles (AGV). La fermentación in vitro realizada midió la degradabilidad de la materia seca, emisiones de CH4 y CO2 a las 24, 48 y 72h. La inclusión de Clitoria ternatea en los ensilados aumentó el contenido de MS y PC (P< 0.05) y disminuyeron (P<0.05) los porcentajes de FDN y FDA. El pH fue menor a 4.5 (P<0.05) con la adición de ácido fosfórico. Las concentraciones de AGV y de NA indicaron buena calidad de los ensilados. La inclusión de 40% de Clitoria ternatea en los ensilados produjo una disminución de 27% de CH4 (P<0.05) durante la fermentación in vitro. Como conclusión, la inclusión de 40% de Clitoria ternatea en ensilados de King grass CT-115 mejora la composición química y disminuye las emisiones de CH4 durante la fermentación ruminal in vitro.

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Armelia Sari Widyarman ◽  
Stephani Sumadi ◽  
Tri Putriany Agustin

  Introduction: Clitoria ternatea flower contains flavonoid such as anthocyanin that gives the blue color to its flower and has antimicrobial activity.   Objectives: The aim of this study was to examine the effect of flower juice of Clitoria ternatea against Porphyromonas gingivalis biofilm viability in vitro.   Methods: This study was experimental laboratory research using biofilm assay method. P. gingivalis was cultured in BHI broth in 37°C for 24h under anaerobic condition. Fresh flowers of Clitoria ternatea were extracted using mortar and pestle and diluted into 6 different concentrations: 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% with phosphate buffer saline (PBS). Chlorhexidine (0.2%) was used as positive control and biofilm without treatment as negative control. The flower juice was distributed into 96 well-plates that contained biofilm of P. gingivalis and incubated for 1h, 3h, 6h, and 24h in 37°C, anaerobic atmosphere. Biofilm was measured using crystal violet dye with microplate reader (490 nm). Data were statistically analysed using one-way ANOVA test and Post Hoc test with p<0.05 was set as significant different.   Result: Result showed that Clitoria ternatea flower juice significantly reduced the Porphyromonas gingivalis biofilm viability in all concentration and all incubation time. The most effective concentration to inhibit Porphyromonas gingivalis biofilm was 100% in 1h incubation time which biofilm was diminished (Optical Density=0.00). One way ANOVA test and Post Hoc test showed a significant biofilm reduction in all concentration and all incubation time after treatment with the flower juice compared to control (p<0.05).   Conclusion: Clitoria ternatea flower juice has antibiofilm effect against Porphyromonas gingivalis. This result showed this flower juice may be useful for combating periodontal pathogens. However, further studies using other bacteria are still needed to confirm this result.


2014 ◽  
Vol 5 (12) ◽  
pp. 926-928 ◽  
Author(s):  
Ranaweera Chathuranga Bharathee ◽  
Pathirana Ranjith ◽  
Ambalanduwa Kankanamge Chandana ◽  
Jayakody Ralalage Anusha Chandra Jayakody ◽  
Ratnasooriya Wanigasekara Daya

2019 ◽  
Vol 295 ◽  
pp. 646-652 ◽  
Author(s):  
Charoonsri Chusak ◽  
Jowynn Ang Yu Ying ◽  
Joseph Lim Zhien ◽  
Porntip Pasukamonset ◽  
Christiani Jeyakumar Henry ◽  
...  

Foods ◽  
2018 ◽  
Vol 7 (7) ◽  
pp. 102 ◽  
Author(s):  
Charoonsri Chusak ◽  
Christiani Henry ◽  
Praew Chantarasinlapin ◽  
Varanya Techasukthavorn ◽  
Sirichai Adisakwattana

Author(s):  
C. B. Ranaweera ◽  
A. K. Chandana

Clitoria ternatea commonly known as Butterfly pea is a standard Ayurvedic medicinal plant used in many parts of south Asian countries. Traditional medicinal plants are a great alternative to find new treatments and for the development of novel antimicrobials to combat many diseases. In Ayurveda and traditional and folk medicine in several countries, decoction and extracts made from C. ternatea are recommended to be used for various medical treatments. C. ternatea extracts claimed to possess antibacterial, antiviral, and antifungal properties, which had been supported and validated by many in vitro and in vivo experiments. However, biologically active compound/s isolation and development novel compounds still remain in its infancy. Despite its enormous potential health benefits, only a single commercial product managed to reach industrial level production. C. ternatea cyclotide studies are also limited despite the fact that it the fastest known natural ligase discovered to date. These cyclotides are rapid peptide ligators and has been the focus of many recent studies on peptide ligation and cyclization for biotechnological applications. In this mini summary we have tried to point out innate unique biological properties of C. ternatea and suggested few future studies, more specifically on C. ternatea cyclotides development against bacterial heat shock proteins (Hsp 100) for novel antimicrobial discovery and development.


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