scholarly journals Etude bactériologique et pathologique de la pneumonie chez le dromadaire (Camelus dromedarius) en Jordanie

2001 ◽  
Vol 54 (2) ◽  
pp. 93 ◽  
Author(s):  
Y. H. Al Tarazi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Mannheimia Haemolytica ◽  
Camelus Dromedarius ◽  
E Coli ◽  
Actinomyces Pyogenes ◽  
Klebsiella Spp

Les publications concernant la pneumonie chez le dromadaire sont en général rares. Dans cette étude, 284 poumons de dromadaires, abattus entre 6 mois et 10 ans d’âge et provenant du nord de la Jordanie, ont été examinés. La prévalence de la pneumonie a été de 10,2 p. 100. Les lésions pathologiques des poumons atteints de pneumonie ont été classées selon qu’elles indiquaient la présence de la bronchopneumonie proliférative chronique, de la pleuropneumonie chronique ou de la pneumonie interstitielle. Les abcès du poumon ont aussi été enregistrés. La broncho-pneumonie proliférative chronique (20,69 p. 100) et la pleuropneumonie chronique (6,9 p. 100) ont été plus fréquentes chez les dromadaires âgés (10 ans environ), alors que la pneumonie interstitielle (58,6 p. 100) et les abcès du poumon (10,34 p. 100) ont été plus fréquents chez les jeunes dromadaires (âgés de 6 mois à 4 ans). Mannheimia haemolytica et Pseudomonas aeruginosa ont été les espèces les plus fréquemment isolées dans le cas de broncho-pneumonie proliférative chronique et de pleuropneumonie chronique, alors que, dans les cas de pneumonie interstitielle, c’étaient Escherichia coli et Klebsiella spp. qui l’ont été le plus. Dans les cas d’abcès du poumon c’étaient Staphylococcus aureus, Actinomyces pyogenes et des streptocoques hémolytiques qui ont été le plus fréquemment observés. Au total, 75 espèces bactériennes ont été isolées de 29 poumons. Les plus fréquentes ont été E. coli (26,66 p. 100), Klebsiella spp. (14,66 p. 100), Pseudomonas aeruginosa (12 p. 100), Staphylococcus aureus (10,66 p. 100), Mannheimia haemolytica (6,66 p. 100) et Actinomyces pyogenes (6,66 p. 100). L’espèce la plus souvent observée parmi les Klebsiella identifiées a été Klebsiella ozaenae.

scholarly journals Prevalence of symptomatic urinary tract infection and bacterial spectrum of diabetic and non-diabetic patients at the two teaching hospitals in Enugu, Nigeria

2021 ◽  
Vol 22 (4) ◽  
pp. 480-488
Author(s):  
C.C. Okwume ◽  
N.F. Onyemelukwe ◽  
I.N. Abdullahi ◽  
O.E. Okoyeocha ◽  
S.D. Asamota
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Pathogen ◽  
Diabetic Patients ◽  
Isolation Rate ◽  
E Coli ◽  
Enterococcus Spp ◽  
Infections Urinaires ◽  
Klebsiella Spp

Background: Diabetes mellitus is a group of metabolic disorder characterized by relative or absolute lack of insulin. When this condition is not properly managed, it can lead to complications that make diabetic patients vulnerable to urinary tract infections (UTI). The objectives of this study are to determine the prevalence of microbiologically confirmed UTI and the spectrum of uropathogens in diabetic and non-diabetic patients with clinical features of UTI attending the two tertiary hospitals in Enugu State, Nigeria. Methodology: Clean catch specimen of single mid-stream urine sample was collected from each of 60 (22 males, 38 females) diabetic and 60 (22 males, 38 females) non-diabetic patients enrolled using stratified random sampling method. The samples were cultured on standard microbiological culture media (MacConkey and Blood agar plates) and incubated aerobically at 37◦C for 24 hours. Plates with significant bacteria growth (>105 CFU/ml) were processed further for bacterial identification using conventional biochemical test scheme. Antibiotic susceptibility test (AST) of each isolate to 17 selected antibiotics was performed by the modified disc diffusion method. Results: Of the total 120 patients enrolled, 101 had bacterial pathogens isolated from their voided urine samples; 51 of 60 (85.0%) diabetics and 50 of 60 (83.3%) non-diabetics (p=0.802). Bacteria were isolated in 59.1% (13/22) of diabetic and 54.5% (12/22) of non-diabetic male patients compared to 100% (38/38) isolation rate in diabetic and non-diabetic female patients. The most frequently isolated bacteria in the diabetic patients were Proteus spp (18.6%), Klebsiella spp (16.9%) and Escherichia coli (15.5%) while the most frequently isolated bacteria among the non-diabetic patients were E. coli (30.0%), Proteus spp (26.3%) and Enterobacter spp (14.0%). Apart from Klebsiella spp which was more frequently isolated from the diabetic (16.9%) than non-diabetic patients (6%) (p=0.039), the frequency other bacterial pathogen isolation such as Proteus spp, E. coli, Enterobacter spp, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus spp was not significantly different between the two population groups (p>0.05). The Gram-positive and Gram-negative bacteria were highly sensitive to imipenem in both diabetic and non-diabetic patients, but the isolates from both study groups exhibited low susceptibility to amoxicillin, nitrofuran- toin, cefixime and cefuroxime. Conclusion: Although the overall frequency of bacterial pathogen isolation in the diabetic and non-diabetic patients was not significantly different, females had a higher pathogen isolation rate than the males, and diabetic females had a higher frequency of polymicrobial infections compared to non-diabetic females and the male population. The high antimicrobial resistance of the isolated bacteria pathogens underscores the need for clinical microbiology laboratory testings to optimize the management of UTI in diabetic patients.   French title: Prévalence des infections urinaires symptomatiques et spectre bactérien des patients diabétiques et non diabétiques dans les deux hôpitaux universitaires d'Enugu, au Nigeria   Contexte: Le diabète sucré est un groupe de troubles métaboliques caractérisés par un manque relatif ou absolu d'insuline. Lorsque cette condition n'est pas correctement gérée, elle peut entraîner des complications qui rendent les patients diabétiques vulnérables aux infections des voies urinaires (UTI). Les objectifs de cette étude sont de déterminer la prévalence des infections urinaires confirmées microbiologiquement et le spectre des uropathogènes chez les patients diabétiques et non diabétiques présentant des caractéristiques cliniques des infections urinaires fréquentant les deux hôpitaux tertiaires de l'État d'Enugu, au Nigeria. Méthodologie: Un échantillon de capture propre d'un seul échantillon d'urine à mi-jet a été prélevé sur chacun des 60 (22 hommes, 38 femmes) patients diabétiques et 60 (22 hommes, 38 femmes) patients non diabétiques inscrits à l'aide d'une méthode d'échantillonnage aléatoire stratifié. Les échantillons ont été cultivés sur des milieux de culture microbiologiques standard (plaques de gélose MacConkey et Blood) et incubés en aérobie à 37°C pendant 24 heures. Les plaques avec une croissance bactérienne significative (>105 CFU/ml) ont été traitées davantage pour l'identification bactérienne en utilisant un schéma de test biochimique conventionnel. Le test de sensibilité aux antibiotiques (AST) de chaque isolat à 17 antibiotiques sélectionnés a été réalisé par la méthode de diffusion sur disque modifiée. Résultats: Sur un total de 120 patients recrutés, 101 avaient des agents pathogènes bactériens isolés de leurs échantillons d'urine évacués; 51 des 60 (85,0%) diabétiques et 50 des 60 (83,3%) des non-diabétiques (p=0,802). Les bactéries ont été isolées chez 59,1% (13/22) des patients diabétiques et 54,5% (12/22) des hommes non diabétiques contre un taux d'isolement de 100% (38/38) chez les femmes diabétiques et non diabétiques. Les bactéries les plus fréquemment isolées chez les patients diabétiques étaient Proteus spp (18,6%), Klebsiella spp (16,9%) et Escherichia coli (15,5%) tandis que les bactéries les plus fréquemment isolées chez les patients non diabétiques étaient E. coli (30,0%), Proteus spp (26,3%) et Enterobacter spp (14,0%). Hormis Klebsiella spp qui était plus fréquemment isolé chez les diabétiques (16,9%) que les patients non diabétiques (6%) (p=0,039), la fréquence d'isolement d'autres agents pathogènes bactériens tels que Proteus spp, E. coli, Enterobacter spp, Pseudomonas aeruginosa, Staphylococcus aureus et Enterococcus spp n'étaient pas significativement différents entre les deux groupes de population (p>0,05). Les bactéries Gram-positives et Gram-négatives étaient très sensibles à l'imipénème chez les patients diabétiques et non diabétiques, mais les isolats des deux groupes d'étude présentaient une faible sensibilité à l'amoxicilline, à la nitrofurantoïne, au céfixime et à la céfuroxime. Conclusion: Bien que la fréquence globale d'isolement des agents pathogènes bactériens chez les patients diabétiques et non diabétiques n'était pas significativement différente, les femmes avaient un taux d'isolement des agents pathogènes plus élevé que les hommes, et les femmes diabétiques avaient une fréquence plus élevée d'infections polymicrobiennes par rapport aux femmes non diabétiques. et la population masculine. La haute résistance antimicrobienne des bactéries pathogènes isolées souligne la nécessité de tests de laboratoire de microbiologie clinique pour optimiser la gestion des infections urinaires chez les patients diabétiques.    

scholarly journals Evaluation of the Antibacterial Effectiveness of Some Antiseptics and Disinfectants

2021 ◽  
Vol 6 (1) ◽  
pp. 175-181
Author(s):  
A. I. Aminu ◽  
◽  
M. S. Abdullahi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
E Coli ◽  
Test Organisms ◽  
Inhibition Zones ◽  
Key Words Evaluation ◽  
Klebsiella Spp

Application of disinfectants and antiseptics continue to be an essential part of infection control practices; as such there is the need for constant evaluation of their effectiveness. The study aimed at evaluating the antibacterial effectiveness of some brands of disinfectants and antiseptics against some bacterial isolates. Two disinfectants-D1 (Sodium hypochlorite 3.85%) and D2 (7% Tar acid, phenol, 2% cresylic creosote) and two antiseptics-A1 (Chloroxyenol B.Pc 48%nw/v, oleumpiniaromgticum 8.3%w/w) and A2 (Dichlorometaxylenol, IPA, terpinol) were selected and their efficacies against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella spp and Escherichia coli were determined using agar disk diffusion methods. The potency of the disinfectants was further evaluated against phenol to establish the phenol coefficient (Pc). The result revealed that both the disinfectants and the antiseptics inhibited the different test organisms at various concentrations producing higher inhibition zones at higher concentrations (P=0.001) that differ significantly with the type of agent used (P=0.007).Both agents produces higher inhibitions zones against S. aureus, Klebsiella spp, E. coli and the least inhibition zones against P. aeruginosa. At 100% concentration, D1 produces the highest inhibition zone of 21mm against E. coli, while A1produces the highest inhibition zones of 17mm against E. coli, S. aureus and Klebsiella spp. The result indicated that both disinfectants (D1 and D2) are more effective than phenol and that D1 is more effective than D2 with Pc coefficient of 3.0 and 2.0 against S. aureus and P. aeruginosa respectively compared to D2 with a Pc of 2.0 and 1.05 against S. aureus and P. aeruginosa. The study identifies that both disinfectants and antiseptics especially D1 and A1 were effective against the test isolates except for P. aeruginosa which was less susceptible. Key words: Evaluation, Disinfectants, Antiseptics, Bacteria

scholarly journals Fabrication of a Novel AgBr/Ag2MoO4@InVO4 Composite with Excellent Visible Light Photocatalytic Property for Antibacterial Use

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1541
Author(s):  
Jie Zhang ◽  
Jia Wang ◽  
Qingjun Zhu ◽  
Binbin Zhang ◽  
Huihui Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Degradation Rate ◽  
Photocatalytic Property ◽  
Photocatalytic Reaction ◽  
Composite Photocatalyst ◽  
E Coli ◽  
Radical Trapping ◽  
Visible Light Photocatalytic

A novel AgBr/Ag2MoO4@InVO4 composite photocatalyst with different heterojunction structures was successfully constructed by compounding InVO4 with Ag2MoO4 and AgBr. According to the degradation, antibacterial and free radical trapping data, the photocatalytic antibacterial and antifouling activities of AgBr/Ag2MoO4@InVO4 composite were evaluated, and the corresponding photocatalytic reaction mechanism was proposed. Adding AgBr/Ag2MoO4@InVO4 composite, the degradation rate of ciprofloxacin (CIP) achieved 95.5% within 120 min. At the same time, the antibacterial rates of Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) achieved 99.99%. The AgBr/Ag2MoO4@InVO4 composite photocatalyst showed promising usage in photocatalytic antibacterial and purification areas.

scholarly journals Estudio fitoquímico y actividad antimicrobiana de plantas medicinales empleadas para el control de infecciones urinarias

2021 ◽  
Vol 16 (1) ◽  
pp. 43-56
Author(s):  
Lida Vanessa Hernández Moreno ◽  
Ludy Cristina Pabón Baquero ◽  
Patricia Hernández-Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Zingiber Officinale ◽  
Taraxacum Officinale ◽  
Portulaca Oleracea ◽  
Uncaria Tomentosa ◽  
E Coli ◽  
Anthoxanthum Odoratum ◽  
Parietaria Officinalis

Las enfermedades infecciosas han sido un desafío para la humanidad. A pesar de los avances en la ciencia, aún muchas infecciones no tienen tratamientos efectivos o los microorganismos han generado resistencia a los antibióticos, de manera que las plantas medicinales son una alternativa de tratamiento y reducción de la resistencia. El objetivo de este estudio fue realizar un análisis fitoquímico preliminar y evaluar la actividad antimicrobiana de doce extractos etanólicos deplantas empleadas en la medicina tradicional colombiana como control de infecciones urinarias (IU). Las especies Anthoxanthum odoratum (grama), Urera caracasana (ortigón), Equisetum bogotenses (cola de caballo), Parietaria officinalis (parietaria), Achyrocline bogotensis (vira vira), Kohleria hirsuta (caracola), Taraxacum officinale (diente de león), Sedum praealtum (siempre viva), Portulaca oleracea (verdolaga), Petroselinum sativum (perejil), Zingiber officinale (jengibre) y Uncaria tomentosa (uña de gato) fueron adquiridas en la plaza de mercado Soacha (Cundinamarca) y sometidas a maceración con etanol a temperatura ambiente. La actividad antimicrobiana se evaluó por difusión en agar, microdilución en placa y bioautografía frente a Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) y Pseudomonas aeruginosa (ATCC 9027). Se determino que S. aureus y P. aeruginosa presentaron inhibición frente a cinco extractos en los tres métodos realizados, mientras que E. coli no fue inhibida por ningún extracto. Se estableció mediante bioautografía con reveladores específicos que los esteroides y/o triterpenoides, fenoles y flavonoides son los posibles metabolitos responsables de la actividad. Este tipo de investigaciones propicia estudios orientados al aislamiento de principios activos con utilidad en la elaboración de medicamentos para tratar IU.

scholarly journals Silver Antibacterial Synergism Activities with Eight Other Metal(loid)-Based Antimicrobials against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus

Antibiotics ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 853
Author(s):  
Ali Pormohammad ◽  
Raymond J. Turner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nitrate ◽  
Zinc Sulfate ◽  
Nickel Sulfate ◽  
Luria Broth ◽  
Potassium Tellurite ◽  
Wound Fluid ◽  
E Coli

The present study surveys potential antibacterial synergism effects of silver nitrate with eight other metal or metalloid-based antimicrobials (MBAs), including silver nitrate, copper (II) sulfate, gallium (III) nitrate, nickel sulfate, hydrogen tetrachloroaurate (III) trihydrate (gold), aluminum sulfate, sodium selenite, potassium tellurite, and zinc sulfate. Bacteriostatic and bactericidal susceptibility testing explored antibacterial synergism potency of 5760 combinations of MBAs against three bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus) in three different media. Silver nitrate in combination with potassium tellurite, zinc sulfate, and tetrachloroaurate trihydrate had remarkable bactericidal and bacteriostatic synergism effects. Synergism properties of MBAs decreased effective antibacterial concentrations remarkably and bacterial cell count decreased by 8.72 log10 colony-forming units (CFU)/mL in E. coli, 9.8 log10 CFU/mL in S. aureus, and 12.3 log10 CFU/mL in P. aeruginosa, compared to each MBA alone. Furthermore, most of the MBA combinations inhibited the recovery of bacteria; for instance, the combination of silver nitrate–tetrachloroaurate against P. aeruginosa inhibited the recovery of bacteria, while three-fold higher concentration of silver nitrate and two-fold higher concentration of tetrachloroaurate were required for inhibition of recovery when used individually. Overall, higher synergism was typically obtained in simulated wound fluid (SWF) rather than laboratory media. Unexpectedly, the combination of A silver nitrate–potassium tellurite had antagonistic bacteriostatic effects in Luria broth (LB) media for all three strains, while the combination of silver nitrate–potassium tellurite had the highest bacteriostatic and bactericidal synergism in SWF. Here, we identify the most effective antibacterial MBAs formulated against each of the Gram-positive and Gram-negative pathogen indicator strains.

scholarly journals Actividad antibacteriana in vitro de aceites esenciales de diferentes especies del género Citrus

2017 ◽  
Vol 46 (2) ◽  
Author(s):  
Miladys Esther Torrenegra Alarcón ◽  
Nerlis Paola Pájaro ◽  
Glicerio León Méndez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
E Coli

Se evaluó la actividad antibacteriana in vitro de aceites esenciales de diferentes especiesdel género Citrus frente a cepas ATCC de Staphylococcus aureus, Staphylococcus epidermidis,Klebsiella pneumoniae, Pseudomonas aeruginosa y Escherichia coli, determinandola concentración mínima inhibitoria (CMI) y la concentración mínima bactericida(CMB). Las bacterias se replicaron en medios de agar y caldos específicos. Se determinóel momento de máxima densidad óptica (DO620) para emplearlo como tiempode incubación; luego se hicieron pruebas de evaluación de sensibilidad con la exposiciónde las cepas a concentraciones a 1000 g/mL del extracto en caldo. Para solubilizarse empleó dimetilsulfóxido (DMSO) al 1%. Posteriormente, se le determinó laconcentración mínima inhibitoria mediante metodologías de microdilución en caldoy la concentración mínima bactericida. Encontrándose una actividad de los aceitesesenciales del género Citrus, con valores de CMI ≥ 600 mg/mL frente a S. aureus,S. epidermidis, K. pneumoniae, P. aeruginosa y E. coli. En función a los resultados obtenidos,se concluye que las diferentes especies del género Citrus son consideradas comopromisorias para el control del componente bacteriano.

scholarly journals Green synthesis of Ag, Zn and Cu nanoparticles from aqueous extract of Spondias mombin leaves and evaluation of their antibacterial activity

2020 ◽  
Vol 21 (2) ◽  
pp. 106-113
Author(s):  
D.K. Adeyemi ◽  
A.O. Adeluola ◽  
M.J. Akinbile ◽  
O.O. Johnson ◽  
G.A. Ayoola
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Green Synthesis ◽  
Antimicrobial Activities ◽  
Cu Nanoparticles ◽  
E Coli ◽  
Spondias Mombin ◽  
Ft Ir ◽  
Uv Visible

Background: Nanotechnology offers an advantage as a green route for synthesis of metal nanoparticles (NPs) with plant extracts as capping agent. Spondias mombin is a fruit-bearing tree and its leaf extracts have been reported to possess anxiolytic, hypoglycaemic, antiepileptic, antipsychotic, sedative, antioxidant, and antimicrobial properties. The objective of the study is to determine the antibacterial potential of a simple non-toxic product of green synthesis of metallic (Ag, Zn and Cu) nanoparticles using the leaf of Spondias mombin aqueous extracts (SMAE) as a reducing and capping agents of the metal ions.Methodology: Nanoparticles were characterized by UV visible spectrophotometeric analysis, Fourier Transform Infra-Red (FT-IR) spectrophotometer and scanning electron microscope (SEM). Antimicrobial activities of synthesized NPs against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were determined by agar well diffusion technique.Results: The synthesized NPs varied in colour from dark brown to green and appears dominantly spherical, occasionally rod or triangular shaped with size ranging from 65-90 nm. UV spectroscopy absorption spectra of Ag, Zn and Cu NPs had absorbance peak at 267, 262 and 765 nm respectively. FT-IR spectrometry of Zn NP, Cu NP, and SMAE gave wave number ranging from 895.71-3320.67, 747.02-3225.45 and 658.25-3674.49 respectively. FT-IR analysis showed that SMAE acted as reducing and stabilizing agent while the NPs exhibited lower energy absorption band when compared to the plant extract. The NPs demonstrated higher antimicrobial activities against S. aureus than Ps. aeruginosa and E. coli. The antimicrobial activity was higher with copper NP than Ag and Zn NPs, and also higher than SMAE.Conclusion: The result from this study presents an indication for an alternative means for development of novel antimicrobial agents for clinical and biotechnological applications.Keywords: synthesis, nanoparticles, FT-IR, UV-visible spectrophotometry, antibacterial activitiesFrench Title: Synthèse verte de nanoparticules d'Ag, de Zn et de Cu à partir d'extrait aqueux de feuilles de Spondias mombin et évaluation de leur activité antibactérienneContexte: La nanotechnologie offre un avantage en tant que voie verte pour la synthèse de nanoparticules métalliques (NP) avec des extraits de plantes comme agent de coiffage. Spondias mombin est un arbre fruitier et ses extraits de feuilles possèdent des propriétés anxiolytiques,  hypoglycémiques, antiépileptiques, antipsychotiques, sédatives,  antioxydantes et antimicrobiennes. L’objectif de l’étude est de déterminer le potentiel antibactérien d’un simple produit non toxique de synthèse verte de nanoparticules métalliques (Ag, Zn et Cu) à l’aide de la feuille d’extraits aqueux de Spondias mombin (SMAE) en tant qu’agent réducteur et coiffant de la ions métalliques.Méthodologie: Les nanoparticules ont été caractérisées par analyse  spectrophotométrique UV visible, spectrophotomètre à transformée de Fourier infrarouge (FT-IR) et microscope électronique à balayage (MEB). Lesactivités antimicrobiennes de NP synthétisées contre Escherichia coli, Staphylococcus aureus et Pseudomonas aeruginosa ont été déterminées par une technique de diffusion sur puits d’agar.Résultats: Les NP synthétisés ont une couleur allant du brun foncé au vert et apparaissent principalement sphériques, parfois en bâtonnets ou en triangles, avec des tailles allant de 65 à 90 nm. Les spectres  d'absorption par spectroscopie UV des NP Ag, Zn et Cu présentaient des pics  d'absorbance à 267, 262 et 765 nm respectivement. La spectrométrie FT-IR de Zn NP, Cu NP et SMAE a donné un nombre d'onde allant de 895,71 à 3320,67, 747,02 à 3225,45 et 658,25 à 3674,49 respectivement. L'analyse FT-IR a montré que le SMAE agissait en tant qu'agent réducteur et  stabilisant, alors que les NP présentaient une bande d'absorption d'énergie inférieure à celle de l'extrait de plante. Les NP ont démontré des activités antimicrobiennes plus élevées contre S. aureus que Ps. aeruginosa et E. coli. L'activité antimicrobienne était plus élevée avec les NP en cuivre que dans les NP Ag et Zn, et également supérieure à celle du SMAE.Conclusion: le résultat de cette étude présente une indication d'un autre moyen de développement de nouveaux agents antimicrobiens pour des applications cliniques et biotechnologiques.Mots-clés: synthèse, nanoparticules, FT-IR, spectrophotométrie UV-visible, activités antibactériennes 

scholarly journals Antibacterial effect of Dimethyl sulfoxide extract of Aloe vera (Aloe barbadensis) leaf gel against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae

Mediscope ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 67-74 ◽  
Author(s):  
Syed Didarul Haque ◽  
Abu Md Mayeenuddin Al Amin ◽  
Baishakhi Islam ◽  
Nazia Nazneen ◽  
Syeda Noorjahan Karim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Dimethyl Sulfoxide ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Biologically Active ◽  
Diffusion Method ◽  
E Coli

An exploratory study based on laboratory experiment was carried out to determine the antibacterial effect of Dimethyl sulfoxide (DMSO) extract of Aloe vera leaf gel (DAE) against standard strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae in the Department of Pharmacology & Therapeutics in collaboration with the Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh. DMSO extract was used in five different concentrations (100, 200, 300, 400 and 500 μg/ml). Dose dependent inhibitory effect was seen against the test organisms using disc diffusion method. Zone of inhibition (ZOI) were 8 mm, 13 mm, 15 mm, 16 mm and 21 mm against S. aureus; 0 mm, 8 mm, 13 mm, 15 mm and 18 mm against P. aeruginosa; 8 mm, 11 mm, 13 mm, 16 mm and 20 mm against E. coli; 0 mm, 9 mm, 12 mm, 14 mm and 18 mm against K. pneumoniae at 100, 200, 300, 400 and 500 μg/ml respectively. The minimum inhibitory concentration (MIC) was assessed by broth dilution technique. The MICs of DAE for S. aureus, P. aeruginosa, E. coli and K. pneumoniae were 300 μg/ml, 400 μg/ml, 400 μg/ml and 450 μg/ml respectively. From the study it was observed that DMSO extract of Aloe vera leaf gel possesses antibacterial effect against the test pathogens. The findings highlight the need for further extensive study to detect and isolate the biologically active ingredients present in the Aloe vera leaves which are responsible for antibacterial effect. Hopefully, that would lead to the discovery of new and more potent antimicrobial agents originated from Aloe vera. Mediscope Vol. 7, No. 2: July 2020, Page 67-74

scholarly journals Affinity of Tomopenem (CS-023) for Penicillin-Binding Proteins in Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa

2008 ◽  
Vol 53 (3) ◽  
pp. 1238-1241 ◽  
Author(s):  
Tetsufumi Koga ◽  
Chika Sugihara ◽  
Masayo Kakuta ◽  
Nobuhisa Masuda ◽  
Eiko Namba ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Binding Proteins ◽  
Binding Protein ◽  
Penicillin Binding Protein ◽  
Penicillin Binding Proteins ◽  
E Coli ◽  
High Affinity

ABSTRACT Tomopenem (formerly CS-023), a novel 1β-methylcarbapenem, exhibited high affinity for penicillin-binding protein (PBP) 2 in Staphylococcus aureus, PBP 2 in Escherichia coli, and PBPs 2 and 3 in Pseudomonas aeruginosa, which are considered major lethal targets. Morphologically, tomopenem induced spherical forms in E. coli and short filamentation with bulges in P. aeruginosa, which correlated with the drug's PBP profiles. The potential of resistance of these bacteria to tomopenem was comparable to that to imipenem.

Serum Inhibitory and Bactericidal Activity of Ciprofloxacin following Intravenous Administration

DICP ◽  
1989 ◽  
Vol 23 (6) ◽  
pp. 456-460
Author(s):  
Michael N. Dudley ◽  
Hilary D. Mandler ◽  
Kenneth H. Mayer ◽  
Stephen H. Zinner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Minimum Inhibitory Concentration ◽  
Bactericidal Activity ◽  
Resistant Strain ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
E Coli ◽  
Dose Levels

Serum inhibitory and bactericidal titers were measured in nine healthy volunteers following single iv doses of ciprofloxacin 100, 150, and 200 mg. The median peak serum bactericidal titer (5 minutes following completion of a 30-minute infusion) against two highly susceptible strains of Escherichia coli ranged between 1:64 and 1:1024 and titers exceeded 1:8 for six hours for all dose levels. The bactericidal titers against two strains of Pseudomonas aeruginosa and a methicillin-resistant strain of Staphylococcus aureus were considerably lower, the median peak being 1:2 at all dose levels. Measured inhibitory and bactericidal titers at five minutes and one hour postinfusion were significantly greater than those predicted (measured serum ciprofloxacin concentration to minimum inhibitory concentration [MIC] or minimum bactericidal concentration [MBC]) for only one strain of E. coli. Intravenous doses of ciprofloxacin 100–200 mg produce high and sustained serum bactericidal titers against highly susceptible bacteria; considerably lower levels of activity are seen against bacteria having higher MICs and MBCs but still considered susceptible to the drug.

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