scholarly journals Identification and quantification of phenolic compounds in pomegranate juices from eight Macedonian cultivars

2019 ◽  
Vol 38 (2) ◽  
pp. 149
Author(s):  
Jasmina Petreska Stanoeva ◽  
Nina Peneva ◽  
Marina Stefova ◽  
Viktor Gjamovski

Punica granatum L. is one of the species enjoying growing interest due to its complex and unique chemical composition that encompasses the presence of anthocyanins, ellagic acid and ellagitannins, gallic acid and gallotannins, proanthocyanidins, flavanols and lignans. This combination is deemed responsible for a wide range of health-promoting biological activities.This study was focused on the analysis of flavonoids, anthocyanins and phenolic acids in eight pomegranate varieties (Punica granatum) from Macedonia, in two consecutive years. Fruits from each cultivar were washed and manually peeled, and the juice was filtered. NaF (8.5 mg) was added to 100 ml juice as a stabilizer. The samples were centrifuged for 15 min at 3000 rpm and analyzed using an HPLC/DAD/MSn method that was optimized for determination of their polyphenolic fingerprints.The dominant anthocyanin in all pomegranate varieties was cyanidin-3-glucoside followed by cyanidin and delphinidin 3,5-diglucoside. From the results, it can be concluded that the content of anthocyanins was higher in 2016 compared to 2017. But in contrast, the total content of non-colored polyphenols was around 10 times lower in 2016 compared to the amount found in the same samples in 2017.

2021 ◽  
Author(s):  
Elham Lashkari

Abstract Effects of storage temperature, elevated CO2 and reduced O2 in the package headspace, packaging material, and their interactions on the total content of anthocyanins and degradation of phenolic compounds in ‘Malase Torsh Saveh’ pomegranate were studied during long storage. The results showed that only storage temperature and its duration had significant effects on the degradation of phenolic compounds. The content of anthocyanins was affected by the single effect of storage time and its 3-way interaction effect with the storage temperature and the surrounding gas composition. Higher amounts of both total anthocyanins and browning pigments were recorded at 2 °C. The Browning index (BI) of the pomegranate extracts changed quadratically with the variations in the total anthocyanins content and linearly with the variations in the content of browning compounds. Thermal dipping treatment and its interaction with the storage time significantly affected the BI values of modified atmosphere packaged pomegranates stored at 6 °C.


Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 230
Author(s):  
Oleh Koshovyi ◽  
Ain Raal ◽  
Igor Kireyev ◽  
Nadiya Tryshchuk ◽  
Tetiana Ilina ◽  
...  

The prospect of creating a new medicine with psychotropic activity is shown as a result of studying the chemical composition and pharmacological activity of modified dry extracts of motherwort (Leonurus cardiaca L.) tincture. The most promising substances were the dry extracts, modified by adding small amounts of arginine, valine, phenylalanine, glycine, lysine, and alanine. A total of 15 main phenolic substances were found in the extracts, and eight of them were identified. There were also 10 hydroxycinnamic acids in these extracts, three of which were identified (chlorogenic, caffeic, and rosmarinic acids). The dominant hydroxycinnamic acids were chlorogenic and caffeic acids. Among flavonoids, catechin, hyperoside, and rutin were identified. It should be noted that the extracts had a significant content of ellagic acid. On the basis of the results of the phytochemical analysis of the extracts, it can be concluded that the composition of phenolic compounds does not differ significantly, and the main differences are related to amino acids, which obviously have an impact on the overall pharmacological effect. The results obtained indicate the presence of anxiolytic activity in the motherwort extracts studied in complex with amino acids. The extracts with glycine, valine, and arginine were more effective in reducing anxiety in animals.


Author(s):  
P. Sreedevi ◽  
K. Vijayalakshmi ◽  
R. Venkateswari

Objective: This study was conducted to assess the phytochemical constituents in Punica granatum L. Leaf extracts (PGLE) using standard methods.Methods: The leaf powder was extracted using solvents namely aqueous, hydroalcohol, ethanol, ethyl acetate and n-hexane. Qualitative and Quantitative phytochemical screenings of PGLE were assessed by standard methods.Results: All the leaf extracts were positive for a wide range of bio-active compounds except n-hexane. The result has showed that the maximum amount of total phenols (394.16 mg/g DW of extract), total tannins (210.5 mg/g DW of extract), flavanoids (147.4 mg/g DW of extract) and total triterpenoids (112 mg/g DW of extract) were noted in ethanolic extract of P. granatum leaf (EPGL). The biological assay revealed that relevant amount of carbohydrate, protein, lipid and alkaloid in EPGL.Conclusion: The findings of this study concluded that the EPGL had potential bioactive substances that may be used as pharmaceutical ingredients for formulation of new or prospective potent drug to cure wide range of metabolic diseases.


LWT ◽  
2021 ◽  
pp. 112136
Author(s):  
Sara Kalantari ◽  
Leila Roufegarinejad ◽  
Sajad Pirsa ◽  
Mehdi Gharekhani ◽  
Mahnaz Tabibiazar

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5946
Author(s):  
Dragana Borjan ◽  
Maja Leitgeb ◽  
Željko Knez ◽  
Maša Knez Hrnčič

According to many reports, phenolic compounds isolated from olive leaves have very good biological activities, especially antimicrobial. Presently, the resistance of microorganisms to antibiotics is greater than ever. Therefore, there are numerous recent papers about alternative solutions for inhibiting their influence on human health. Olive leaf is studied as an important source of antimicrobials with low cost and used in medicine. Numerous publications on involving green technologies for isolation of active compounds from olive leaves have appeared over the past few decades. The present review reports on current knowledge of the most isolated phenolic compounds from olive leaf extract as well as methods for their isolation and characterization. This paper uses recent research findings with a wide range of study models to describe the antimicrobial potential of phenolic compounds. It also describes the vast range of information about methods for determination of antimicrobial potential focusing on effects on different microbes. Additionally, it serves to highlight the role of olive leaf extract as an antioxidants and presents methods for determination of antioxidant potential. Furthermore, it provides an overview of presence of enzymes. The significance of olive leaves as industrial and agricultural waste is emphasized by means of explaining their availability, therapeutic and nutritional effects, and research conducted on this field.


2018 ◽  
Vol 21 (3-4) ◽  
pp. 171-180
Author(s):  
Mira Radunić ◽  
Ela Šimera ◽  
Katarina Lozo ◽  
Jelena Gadže ◽  
Maja Jukić Špika

Fruit samples of ‘Granada’, ‘Wonderful’ and ‘Hicaznar’ cultivars were collected in productive pomegranate orchard in Neretva valley, Metković (Croatia) and their pomological and chemical properties were compared. Significant differences among cultivars were noted between the studied traits. Fruit weight ranged from 429.37 g to 359.43 g and the lowest was recorded in cv. ‘Hicaznar’. Color of peel, aril and juice of all investigated cultivars was intensively red and dark red, respectively. ‘Granada’ and ‘Wonderful’ stood out with high juice yield compared with ‘Hicaznar’. Total soluble solid (TSS) of ‘Hicaznar’ (18.61%) was higher than that of ‘Wonderful’ (16.64%). Total acidity (TA) significantly differed among cultivars and ranged from 1.6% to 2.1%. According to the TSS/TA ratio investigated cultivars belong to the group of sour-sweet cultivars. Dissimilarity between cultivars was found in the phenolics content (TPC). It ranged from 679.6 to 906.6 mg/100 g D.W. where ‘Hicaznar’ stood out as a cultivar with highest TPC and also highest flavonoid and non-flavonoid content (521.1 mg/100 g D.W. and 385.5 mg/100 g D.W., respectively). In ‘Granada’ antioxidant activity measured by 1,1-diphenyl-2-picrylhydrazyl was recorded for 64.1%, in ‘Wonderful’ 70.2% and the highest in ‘Hicaznar’ (74.5%). Results of this assessment are the basis for practical determination of the pomegranate use.


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