scholarly journals Quantitative Analysis of Histidine Amino Acid as a Predictor of Ergothioneine in the Drying and Frying Process of White Oyster Mushrooms (Pleurotus ostreatus)

2021 ◽  
Vol 5 (4) ◽  
pp. 360
Author(s):  
Dilla Dayanti ◽  
Windi Permatasari ◽  
Icha Khaerunnisa ◽  
Sri Winarni
Keyword(s):  
Amino Acid ◽  
Pleurotus Ostreatus ◽  
High Performance ◽  
Amino Acid Content ◽  
The Body ◽  
Frying Time ◽  
Drying Time ◽  
Frying Process ◽  
Oyster Mushrooms ◽  
Methionine Content

ABSTRACTBackgrounds: White oyster mushroom (Pleurotus ostreatus) is widely cultivated by Indonesian people due to its delicious and nutritious taste. Pleurotus ostreatus contains 18 amino acids that make up the body and antioxidants, including phenolic compounds and ergothioneine. Ergothioneine as a strong antioxidant is an amino acid derived from histidine which has sulfur groups such as cysteine and methionine. The increase in histidine indicates the activity of ergothioneine.Objectives: This study aims to determine the amino acid content of histidine as a predictor of ergothioneine amino acid in the drying and frying process of white oyster mushrooms.Methods: The extraction method used in this study was maceration with 90% ethanol for drying samples and 70% ethanol for frying samples analyzed using High Performance Liquid Chromatography (HPLC).Results: The results showed that the histidine content increased with the longer drying time, and decreased with the longer frying time. Drying with a variation of 2 days, 3 days, and 5 days were 674788,802 mg/L; 615302.747 mg/L; and 1946113,494 g / L respectively. Frying with a variation of 2 minutes, 3 minutes, and 5 minutes were 500435,148 mg / L; 232428,391 mg/L; and 0.000 mg / L. On the other hand, the methionine content decreased with the longer drying time, and increased with the longer frying time. Drying with a variation of 2 days, 3 days, and 5 days in a row were 6673,283mg/L; 6671,920 mg/L; and 1876,358 mg / L. Frying with a variation of 2 minutes, 3 minutes, and 5 minutes were 2296,698 mg / L; 1243,911 mg/L; and 34764.534 mg/L.Conclusions: the study concludes that the content of histidine as the highest ergothioneine predictor is at drying for 5 days and frying for 2 days.

scholarly journals STUDY OF THE AMINO ACID AND ELEMENTAL COMPOSITION OF THE COMPLEX DRY EXTRACT "PHY-TOINFLAM"

2021 ◽  
pp. 217-223
Author(s):  
Ekut Saidkarimovna Karieva ◽  
Nodira Nigmatillaevna Gaipova ◽  
Kamola Negmatilloevna Nuridullaeva
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Elemental Composition ◽  
Inductively Coupled Plasma ◽  
High Performance ◽  
Amino Acid Content ◽  
The Body ◽  
Total Amino Acid ◽  
Dry Extract

The purpose of this research is to study the amino acid and elemental composition of the complex dry extract "Phytoinflam" obtained from ordinary Oak (latin. Quercus robur L.). Chamomile (latin. Chamоmilla recutita (L.)) and three-lobe Beggarticks (latin. Bidens tripartita). Determination of the amino acid composition was carried out by high performance liquid chromatography (HPLC), and the quantitative determination of macro-and microelements - by the method of inductively coupled plasma mass spectrometry (ICP-MS) According to the results of the research, it was revealed that 20 amino acids were found in the dry extract "Phytoinflam": 15 of which are representatives of various classes of aliphatic amino acids, 3 – aromatic and 2 – heterocyclic. It should be noted that ten out of twenty discovered amino acids are irreplaceable. The largest quantities are proline, valine, alanine, arginine, cysteine and glutamine. The total amino acid content was 14.767 mg/100 mg, of which 38.97% were essential amino acids, and, accordingly, 61.03% were non-essential. The results of the study of micro- and macroelements showed the presence of 28 elements in the analyzed extract, among which seven are essential (Co, Cr, Cu, Fe, Mn, Se, Zn) and four conditionally essential. It is noted that the high content of such elements important for the vital activity of the body as calcium, potassium, sodium, magnesium, silver, strontium. The concentrations of heavy metals and arsenic in the analyzed dry extract did not exceed the regulated limit according to WHO and state Pharmacopoeia XIV. The data obtained indicate the high value of the complex dry extract "Phytoinflam", which confirms its therapeutic value and the possibility of creating drugs based on it.

Amino Acids Profiling in Fruit Juices by High Performance Liquid Chromatography: A Review

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2756-2767
Author(s):  
Vijaya Vemani ◽  
Mounika P ◽  
Poulami Das ◽  
Anand Kumar Tengli
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Amino Acid Content ◽  
Building Blocks ◽  
Fruit Juices ◽  
The Body ◽  
Total Amino Acid ◽  
Analytical Approaches

In the preservation of normal physiological functions, the building blocks of the body called amino acids play a crucial role. A number of valuable and nutritional phytoconstituents are contained in fruit juices, such as vitamins, minerals, microelements, organic acids, antioxidants, flavonoids, amino acids and other components. Due to the growing population and demand, the quality of fruit juices is decreasing. One of the unethical and harmful practices called adulteration or food fraudulence has been adopted by most food and beverage industries. The amino acids which is one of the most important phytochemicals of fruit and fruit juices which affects the organoleptic properties like color, odor, and taste of juices and also helps in authenticity process from governing bodies by providing total amino acid content. Consequently, the main aim of the present review work is to provide information regarding the importance of amino acids, how they are adulterated, the potential analytical approach to detected amino acids and which methods are generally accepted method by the food industries. According to the literature review, we presume that reverse phased high-performance liquid chromatography with pre-column derivatization was the most adopted method for quality checking due to its advantages over other old and recent analytical approaches like simple, rapid, cost-effective nature, less / no sample matrix effect with high sensitivity, accuracy, and precision.

scholarly journals The formulation of white oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) as natural flavoring and the quality test in temperature and drying time variations

2021 ◽  
Vol 922 (1) ◽  
pp. 012054
Author(s):  
S Sadli ◽  
S Saleha ◽  
D Fiana ◽  
M Misrahanum
Keyword(s):  
Low Temperatures ◽  
Pleurotus Ostreatus ◽  
Raw Material ◽  
Quality Test ◽  
Drying Time ◽  
Test Parameters ◽  
Time Variations ◽  
Oyster Mushrooms

Abstract The determination of the drying temperature and time depends to the characteristic of the mushrooms. The use of low temperatures causes the mushrooms to not fully ripen, if the temperature is too high the protein contained in the mushrooms can be denatured and drying too long will change the color of the mushrooms to brown and change its taste. Research on the processing of oyster mushrooms as a raw material for natural flavoring, it is carried out to determine the quality flavoring produced through a descriptive test of the test parameters, color, aroma, taste and texture by variations the temperature 60¼C and 70¼C in drying time 6, 7 and 8 hours. The results of descriptive test of the sixth color of the flavoring have an intensity of attractive colors are light brown; Descriptive taste test F1, F2 and F5 have the intensity of the taste is rather pleasant, the umami is weak while F3, F4 and F6 have taste is quite good, umami tastes; Descriptive test of the six flavoring textures has a good intensity and; Descriptive test of the sixth aroma of flavoring has a slightly fragrant aroma intensity, the aroma of the material is not too smelly. The conclusion of the research is that temperature and drying time do not affect quality of color, aroma and texture. However, they affect the taste where the temperature 60¼C for 8 hours and a temperature of 70¼C within 7 and 8 hours have enough delicious taste with tasteful umami.

scholarly journals Application of Near-Infrared Spectroscopy to Investigate Some Endogenic Properties of Pleurotus ostreatus Cultivars

Sensors ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 6632
Author(s):  
Marietta Fodor ◽  
Erika Etelka Mikola ◽  
András Geösel ◽  
Éva Stefanovits-Bányai ◽  
Zsuzsanna Mednyánszky
Keyword(s):  
Amino Acid ◽  
Free Amino Acid ◽  
Pleurotus Ostreatus ◽  
Free Amino ◽  
Near Infrared ◽  
Amino Acid Content ◽  
Principal Component ◽  
Automatic Amino Acid Analyzer ◽  
Linear Discriminant ◽  
Reducing Ability

Fourteen different Pleurotus ostreatus cultivars (Po_1–Po_14) were tested for free amino acid content (fAA), total polyphenol content (TPC), and antioxidant capacity (Ferric Reducing Ability of Plasma—FRAP) to select the cultivars with the most favorable traits. Automatic amino acid analyzer (fAA) and spectrophotometric assay (TPC, FRAP) results as well as Fourier-transform near infrared (FT-NIR) spectra were evaluated with different chemometric methods (Kruskal–Wallis test, Principal Component Analysis—PCA, Linear Discriminant Analysis—LDA). Based on total free amino acid concentrations and FRAP values, the Po_2 cultivar was found to be the most favorable. Types Po_3, Po_8, Po_10 and Po_12 were separated using PCA. Based on the spectral profile, they may contain polyphenols and reducing compounds of different qualities. LDA classification that was based on the concentrations of all free amino acids, cysteine, and proline of the cultivars was performed with an accuracy of over 90%. LDA classification that was based on the TPC and FRAP values was performed with an accuracy of over 83%.

The interaction of intestinal bacteria and feed: a case study of intensive-duck husbandry in Central Java

2021 ◽  
Vol 46 (2) ◽  
pp. 154-165
Author(s):  
R. Susanti ◽  
M. Dafip ◽  
W. Christijanti ◽  
A. Yuniastuti
Keyword(s):  
Amino Acid ◽  
Essential Amino Acid ◽  
High Performance ◽  
Amino Acid Content ◽  
Intestinal Bacteria ◽  
Vital Role ◽  
Microbiome Analysis ◽  
Central Java ◽  
Intestinal Contents

This study was aimed to compare sugars and amino acid content in duck’s feed and the intestinal bacteria's effects to improve ducks' quality. This research was an observational exploration involving five duck husbandries from Semarang, Temanggung, Magelang, Pati, and Salatiga District, Central Java. A total of 5 g of intestinal contents were collected from each of the five ducks randomly selected from each husbandry. The feed and intestinal contents were then analyzed using high-performance liquid chromatography (HPLC). The highest amino acid types were glutamate, aspartate, lysine, valine, and isoleucine, while the lowest was asparagine found in all sample farms. Intestinal contents freshly extracted then placed in dark bottles sterile for microbiome analysis with primers 6S rRNA V3-V4 genome identification. The concentration of glucose, fructose, and galactose in the intestine has increased significantly caused by digested polysaccharides. The composition of bacteria plays a vital role in digesting polysaccharides, makes them quickly absorbed by duck’s intestine cells. The abundance of bacteria in all sample locations was dominated by the phylum Firmicutes, especially Lactobacilalles, Bacilalles, and Clostridialles. Over feeding may not effective in sugar and essential amino acid absorption, however, it may play an essential role in the diversity of gut bacteria to produce necessary component for duck’s physiology.

scholarly journals Amino Acid and Vitamin Content of Propolis Collected by Native Caucasican Honeybees

2016 ◽  
Vol 60 (2) ◽  
pp. 101-110 ◽  
Author(s):  
Nazife Eroglu ◽  
Senem Akkus ◽  
Mustafa Yaman ◽  
Baris Asci ◽  
Sibel Silici
Keyword(s):  
Liquid Chromatography ◽  
Amino Acid ◽  
Acid Content ◽  
High Performance ◽  
Amino Acid Content ◽  
Biological Properties ◽  
Chemical Components ◽  
Plant Metabolites ◽  
Secondary Plant Metabolites ◽  
Therapeutic Properties

Abstract The polyphenol content of propolis has received a lot of attention due to the benign biological properties noted in the chemical composition studies. However, there are very limited studies about other chemical components found in trace amounts in nature which contribute to the therapeutic properties of propolis. The present study, therefore, investigated the amino acid and vitamin composition of propolis. Propolis harvested by 60 colonies of Apis mellifera caucasica belonged to local non-migratory beekeepers. The A. m. caucasica is known for its distinctive propolis collecting capability which native to the secluded Ardahan Province of Turkey. Vitamin (Thiamine, Riboflavin) combinations of propolis were determined using the HPLC (High-Performance Liquid Chromatography) fluorescent detector. An amino acid analysis was also performed with the UFLC (Ultra-Fast Liquid Chromatography) system consisting of binary pump and UV/VIS. Our findings record that the vitamin and amino acid content of propolis samples collected from three areas of different altitudes in the same region differed from each other. Vitamin B1 content and Vitamin B2 content ranged between 0.025-0.16 mg/100g, and 0.304-0.777mg/100g, respectively. A maximum amount of amino acid was reported as leucine, while a minimum amount of amino acid was seen as tryptophan in Ardahan propolis. Consequently, the vitamin and amino acid content of propolis, which derived from secondary plant metabolites of resin, varied depending on their geographical altitudes. Those vitamin and amino acids found in the propolis composition are believed to have beneficial therapeutic properties.

scholarly journals Free amino acids as phagostimulants in cricket nuptial gifts: support for the ‘Candymaker’ hypothesis

2009 ◽  
Vol 5 (2) ◽  
pp. 194-196 ◽  
Author(s):  
Stuart Warwick ◽  
Karim Vahed ◽  
David Raubenheimer ◽  
Stephen J Simpson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
High Performance ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Nuptial Gifts

Nuptial gifts that are manufactured by the male are found in numerous insect species and some spiders, but there have been very few studies of the composition of such gifts. If, as has been proposed recently, nuptial gifts represent sensory traps, males will be selected to produce gifts that are attractive to females but such gifts will not necessarily provide the female with nutritional benefits (the ‘Candymaker’ hypothesis). We examined the free amino acid content of the spermatophylax of the cricket Gryllodes sigillatus (Orthoptera: Gryllidae) using high performance liquid chromatography (HPLC). The spermatophylax (dry weight) consisted of approximately 7 per cent free amino acids. The free amino acid composition was highly imbalanced, with a low proportion of essential amino acids (18.7%) and a high proportion of proline and glycine. The main free amino acids found in the spermatophylax appeared to act as phagostimulants: the duration of feeding on artificial gels by females was positively related to the free amino acid content of the gels. The results therefore suggest that males use free amino acids to ‘sweeten’ a relatively low-value food item. A possible function of glycine in inhibiting female movement is also proposed.

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