scholarly journals NUCLEOTIDE VARIANCE OF MITOCHONDRIAL DNA D-Loop 126 bp (nt: 34-159) REGION IN MADURESE

2017 ◽  
Vol 52 (2) ◽  
pp. 80
Author(s):  
Nilly Sulistyorini ◽  
Ahmad Yudianto ◽  
Nola Margaret
Keyword(s):  
Rural Areas ◽  
Nuclear Dna ◽  
Past History ◽  
Evolutionary Process ◽  
Genetic Variations ◽  
Insertion Mutation ◽  
Nucleotide Substitutions ◽  
Transversion Mutation ◽  
D Loop ◽  
History Of

Endogamy continues to occur among the Madurese people in rural areas of the island of Madura, especially those areas of the smallest islands around the mainland of Madura. Endogamy as seen from a genetic standpoint will increase the frequency of homozygous genotypes. With regard to genetic variations, STRs of nuclear DNA and polymorphisms in mtDNA are frequently examined. Mitochondrial variations in the human undergo an evolutionary process through the accumulation of changes in DNA sequence, i.e. through the process of nucleotide substitutions that evolves in number with the directional development of lineage. So far, the genetic variations among the populations in Madura Island have not been known. The present study was an observational analytical research with the purpose of determining the genetic variations in the polymorphisms of 126-bp mtDNA D-Loop HV2 (nt: 34-159) in the populations of Madura Island. Results indicated that, based on the homology analysis with rCRS sequence, there were 9 variants consisting of two transition mutations, 6 transversion mutations, and one insertion mutation. This indicates that a transversion mutation had a higher probability than transition and insertion mutations. According to Mustama (2007), a gene pool is not only a collection of genes but a dynamic system organized and containing the past history of a population. Any genetic information has certain historical, anthropological and statistical aspects necessitating an interdisciplinary coordination and collaboration.

scholarly journals CODIS STR LOCI (CSF1PO, THOI, TPOX, vWA) GENETIC VARIATION ANALYSIS IN MADURESE

2017 ◽  
Vol 52 (1) ◽  
pp. 1
Author(s):  
Ahmad Yudianto ◽  
Agung Sosiawan ◽  
Nola Margaret
Keyword(s):  
Rural Areas ◽  
Nuclear Dna ◽  
Past History ◽  
Evolutionary Process ◽  
Genetic Variations ◽  
Nucleotide Substitutions ◽  
The Past ◽  
History Of ◽  
Analytical Research ◽  
Coordination And Collaboration

Endogamy continues to occur among the Madurese people in rural areas of the island of Madura, especially those areas of the smallest islands around the mainland of Madura. Endogamy as seen from a genetic standpoint will increase the frequency of homozygote genotypes. With regard to genetic variations, STRs of nuclear DNA and polymorphisms in mtDNA are frequently examined. Genetic variations in human undergo an evolutionary process through the accumulation of changes in DNA sequence, i.e. through the process of nucleotide substitutions that evolves in number with the directional development of lineage. So far, the genetic variations among the populations in Madura Island have not been known. The present study was an observational analytical research with the purpose of determining the genetic variations in STR CODIS in the populations of Madura Island. Results indicated that, based on loci alelle: CSF1PO, THOI, TPOX, and vWA, there was homozygote genotypes. The allele variations is not specific for Madurese ethnic but this variations may represent married model in Madurese ethnic. According to Mustama (2007), a gene pool is not only a collection of genes but a dynamic system organized and containing the past history of a population. Any genetic information has certain historical, anthropological and statistical aspects necessitating an interdisciplinary coordination and collaboration.

scholarly journals Development, validation, and pilot implementation of the minimum datasheet for a domestic violence registry system: The case of a developing country

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261460
Author(s):  
Shabnam Iezadi ◽  
Kamal Gholipour ◽  
Ahmad Khanijahani ◽  
Mahasti Alizadeh ◽  
Bahram Samadirad ◽  
...  
Keyword(s):  
Domestic Violence ◽  
Sexual Violence ◽  
Rural Areas ◽  
Content Validity ◽  
Past History ◽  
The Elderly ◽  
World Health ◽  
Registry System ◽  
History Of ◽  
Pilot Implementation

Background Domestic violence (DV) is a universal issue and an important public health priority. Establishing a DV Registry System (DVRS) can help to systematically integrate data from several sources and provide valid and reliable information on the scope and severity of harms. The main objective of this study was to develop, validate, and pilot-test a minimum datasheet for a DVRS to register DV victims in medical facilities. Materials and methods This study was conducted in two main phases. Phase one includes developing the datasheet for registration of DV in the DVRS. In phase two, the datasheet designed in the previous step was used in a pilot implementation of the DVRS for 12 months to find practical challenges. The preliminary datasheet was first developed using information on similar registry programs and guidelines of the World Health Organization (WHO) and then reviewed by four expert panels. Through a two-round Delphi technique, experts evaluated the instrument using the Content Validity Index (CVI) and Content Validity Ratio (CVR). The consistency of the responses was evaluated by test-retest analysis. Finally, two physicians in two forensic medical clinics registered the victims of physical and/or sexual violence perpetrated by a family member. Results Preliminary datasheet consisted of 31 items. In the first round of Delphi, fifteen items had good content validity (I-CVI and CVR) and were kept, and seven items were moved to the next round. Also, in the first round of Delphi, experts suggested adding three items, including history of the violence, custody of the child, and custody of the elderly. All items evaluated in the second round were kept due to good CVR and CVI scores. As a result of Test-retest correlation coefficients for self-reprted items, two items including perpetrator’s alcohol and drug use status were excluded (r(30) = +.43, and +.38, p< .01, two-tailed, respectively). Finally, 24 items were included in the datasheet including 15 items for individuals’ characteristics (victims’ characteristics and perpetrators’ characteristics), eight items for incidents’ characteristics, and one item for past history of violence experience. A total of 369 cases were registered from September 23, 2019, to July 21, 2020. The majority of the reported cases were female (82%) and were 19–40 years old. No physical and/or sexual violence was reported from rural areas, which calls upon researchers to explore how services for detecting and treating the victims can be made accessible to these areas. Conclusion DVRS can show trends in DV by age, sex, the context of the violence, and incidence characteristics at every point in time. This is particularly valuable in planning and prioritizing research areas and interventions for DV prevention. Additionally, DVRS can be linked to other disease registry programs which can contribute to continuity and coordination of care, and major research in the future. Although a DVRS can be a promising initiative in identifying the areas in need of urgent interventions, there is no guarantee for its proper implementation due to limited resources and other challenges.

scholarly journals Prevalence and risk factors of superficial fungal infections among primary school pupils in Ilorin, North Central Nigeria

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Louis Odeigah ◽  
Stella Rotifa ◽  
Rasaq Shittu ◽  
Yahkub Mutalub
Keyword(s):  
Risk Factors ◽  
Local Government ◽  
Primary School ◽  
Fungal Infection ◽  
Rural Areas ◽  
Fungal Infections ◽  
Past History ◽  
Local Government Area ◽  
History Of ◽  
Primary School Pupils

Superficial fungal infections are common in the tropics particularly in the rural areas where children are predisposed. The causative organisms include dermatophytes, yeasts and non-dermatophyte moulds. To determine the prevalence and risk factors of superficial fungal infections among primary school pupils aged 5- 16 years in Oke-Oyi, Kwara State. A cross-sectional survey was carried out from April through July 2017 among 602 pupils aged 5-16 years in Oke-Oyi, Ilorin East Local Government Area of Kwara State. Out of the 602 pupils screened during the study, 180 pupils were suspected clinically to have superficial fungal infection and a total of 180 skin specimens were collected from the pupils. The prevalence of clinically suspected fungal infection was 29.9% (180/602), dermatophytes accounted for 16.7% (30/180), while non-dermatophyte moulds accounted for about half of the isolates, 51.7% (93/180). Some of the factors that were significantly associated with the risk of acquisition of dermatophytic infections include age, past history of similar lesions, over-crowding, normal sweat pattern and unkempt socks worn by the pupils among others. This study has shown that superficial fungal infections are common among pupils in Oke-Oyi in Ilorin East Local Government Area of Kwara State and the risk factors include among others, young age, past history of skin infections, overcrowding and unkempt socks worn by pupils. Therefore sociodemographic and behavioural factors influence the occurrence of superficial fungal infections in the study area.

scholarly journals Study on genetic variations of the D-loop region in three Vietnamese ethnic groups Kinh, Lolo and Lahu

2020 ◽  
Vol 18 (2) ◽  
pp. 231-238
Author(s):  
Nguyen Thuy Duong ◽  
Nguyen Van Phong ◽  
Nguyen Thy Ngoc ◽  
Nong Van Hai
Keyword(s):  
Ethnic Groups ◽  
Genetic Distances ◽  
Genetic Variations ◽  
Language Group ◽  
Nucleotide Polymorphisms ◽  
Loop Region ◽  
Minority Ethnic Groups ◽  
Mtdna Sequence ◽  
D Loop ◽  
History Of

Mitochondrial DNA (mtDNA) sequence analysis has been widely used to investigate population genetic and evolutional history of different ethnic groups worldwide. In this study, the D-loop region of 119 Vietnamese individuals belonging to three different ethnic groups was sequenced and compared with reference mtDNA on rCRS for genetic variations. Total 218 genetic polymorphisms were found in this population, among which 48 variations appeared with frequencies of more than 0.1. Further statistical analysis showed that there were 23, 13 and 24 single nucleotide polymorphisms (SNP) distributed differently between Kinh vs. Lolo, Kinh  vs. Lahu , and Lolo  vs. Lahu groups, respectively. The mean pairwise genetic distances between each pair of ethnic groups were 0.0101, 0.0098 and 0.0092 for Kinh - Lolo, Kinh - Lahu, Lolo - Lahu, respectively. This indicated that although the Lolo and Lahu ethnic groups both belong to the Tibeto-Burman language group, the genetic distance between them was no closer than the genetic distances between them and the Kinh group of the Viet-Muong (Austroasiatic) language group. This study was the first research on the D-loop region of the Lolo and Lahu populations in Vietnam. These results provided more data for exploring the genetic background and the history of those ethnic groups as well as other minority ethnic groups in Vietnam.

PREVALENCE AND RELATED FACTORS OF POSTNATAL DEPRESSION IN HIV INFECTED WOMEN

2014 ◽  
pp. 140-152
Author(s):  
Manh Hoan Nguyen ◽  
Ngoc Thanh Cao
Keyword(s):  
Regression Analysis ◽  
Postpartum Depression ◽  
Hiv Infection ◽  
Postnatal Depression ◽  
Past History ◽  
Depression Scale ◽  
Multivariate Regression Analysis ◽  
Related Factors ◽  
History Of ◽  
History Of Depression

Background and Objective: HIV infection is also a cause of postpartum depression, however, in Vietnam, there has not yet the prevalence of postpartum depression in HIV infected women. The objective is to determine prevalence and related factors of postpartum depression in HIV infected women. Materials and Methods: From November 30th, 2012 to March 30th, 2014, a prospective cohort study is done at Dong Nai and Binh Duong province. The sample includes135 HIV infected women and 405 non infected women (ratio 1/3) who accepted to participate to the research. We used “Edinburgh Postnatal Depression Scale (EPDS) as a screening test when women hospitalized for delivery and 1 week, 6weeks postpartum. Mother who score EPDS ≥ 13 are likely to be suffering from depression. We exclude women who have EPDS ≥ 13 since just hospitalize. Data are collected by a structural questionaire. Results: At 6 weeks postpartum, prevalence of depression in HIV infected women is 61%, in the HIV non infected women is 8.7% (p < 0.001). There are statistical significant differences (p<0.05) between two groups for some factors: education, profession, income, past history of depression, child’s health, breast feeding. Logistical regression analysis determine these factors are related with depression: late diagnosis of HIV infection, child infected of HIV, feeling guilty of HIV infected and feeling guilty with their family. Multivariate regression analysis showed 4 factors are related with depression: HIV infection, living in the province, child’s health, past history of depression. Conclusion: Prevalence of postpartum depression in HIV infected women is 61.2%; risk of depression of postnatal HIV infected women is 6.4 times the risk of postnatal HIV non infected women, RR=6.4 (95% CI:4.3 – 9.4). Domestic women have lower risk than immigrant women from other province, RR=0.72 (95% CI:0.5 – 0.9). Past history of depression is a risk factor with RR=1.7 (95% CI:1.02 – 0.9. Women whose child is weak or die, RR=1.7(95% CI:0.9 – 3.1). Keywords: Postpartum depression, HIV-positive postpartum women

Nonlocal Gravity

2017 ◽  
Author(s):  
Bahram Mashhoon
Keyword(s):  
Gravitational Field ◽  
Gravitational Lensing ◽  
Past History ◽  
Lorentz Invariance ◽  
Minkowski Spacetime ◽  
Field Measurements ◽  
Local Principle ◽  
History Of ◽  
Expansion Of The Universe ◽  
Nearby Galaxies

A postulate of locality permeates through the special and general theories of relativity. First, Lorentz invariance is extended in a pointwise manner to actual, namely, accelerated observers in Minkowski spacetime. This hypothesis of locality is then employed crucially in Einstein’s local principle of equivalence to render observers pointwise inertial in a gravitational field. Field measurements are intrinsically nonlocal, however. To go beyond the locality postulate in Minkowski spacetime, the past history of the accelerated observer must be taken into account in accordance with the Bohr-Rosenfeld principle. The observer in general carries the memory of its past acceleration. The deep connection between inertia and gravitation suggests that gravity could be nonlocal as well and in nonlocal gravity the fading gravitational memory of past events must then be taken into account. Along this line of thought, a classical nonlocal generalization of Einstein’s theory of gravitation has recently been developed. In this nonlocal gravity (NLG) theory, the gravitational field is local, but satisfies a partial integro-differential field equation. A significant observational consequence of this theory is that the nonlocal aspect of gravity appears to simulate dark matter. The implications of NLG are explored in this book for gravitational lensing, gravitational radiation, the gravitational physics of the Solar System and the internal dynamics of nearby galaxies as well as clusters of galaxies. This approach is extended to nonlocal Newtonian cosmology, where the attraction of gravity fades with the expansion of the universe. Thus far only some of the consequences of NLG have been compared with observation.

scholarly journals Low Nucleotide Diversity in Chimpanzees and Bonobos

Genetics ◽  
2003 ◽  
Vol 164 (4) ◽  
pp. 1511-1518 ◽  
Author(s):  
Ning Yu ◽  
Michael I Jensen-Seaman ◽  
Leona Chemnick ◽  
Judith R Kidd ◽  
Amos S Deinard ◽  
...  
Keyword(s):  
Nucleotide Diversity ◽  
Nuclear Dna ◽  
Demographic History ◽  
Nuclear Genome ◽  
Limited Data ◽  
Effective Population ◽  
East Central ◽  
Dna Diversity ◽  
History Of ◽  
Mtdna Diversity

Abstract Comparison of the levels of nucleotide diversity in humans and apes may provide much insight into the mechanisms of maintenance of DNA polymorphism and the demographic history of these organisms. In the past, abundant mitochondrial DNA (mtDNA) polymorphism data indicated that nucleotide diversity (π) is more than threefold higher in chimpanzees than in humans. Furthermore, it has recently been claimed, on the basis of limited data, that this is also true for nuclear DNA. In this study we sequenced 50 noncoding, nonrepetitive DNA segments randomly chosen from the nuclear genome in 9 bonobos and 17 chimpanzees. Surprisingly, the π value for bonobos is only 0.078%, even somewhat lower than that (0.088%) for humans for the same 50 segments. The π values are 0.092, 0.130, and 0.082% for East, Central, and West African chimpanzees, respectively, and 0.132% for all chimpanzees. These values are similar to or at most only 1.5 times higher than that for humans. The much larger difference in mtDNA diversity than in nuclear DNA diversity between humans and chimpanzees is puzzling. We speculate that it is due mainly to a reduction in effective population size (Ne) in the human lineage after the human-chimpanzee divergence, because a reduction in Ne has a stronger effect on mtDNA diversity than on nuclear DNA diversity.

scholarly journals LENGTH MUTATIONS IN HUMAN MITOCHONDRIAL DNA

Genetics ◽  
1983 ◽  
Vol 104 (4) ◽  
pp. 699-711
Author(s):  
R L Cann ◽  
A C Wilson
Keyword(s):  
Nuclear Dna ◽  
Average Rate ◽  
Rapid Evolution ◽  
Human Species ◽  
Base Pairs ◽  
Human Mitochondrial Dna ◽  
Noncoding Sequences ◽  
Mitochondrial Dnas ◽  
D Loop ◽  
Length Mutations

ABSTRACT By high-resolution, restriction mapping of mitochondrial DNAs purified from 112 human individuals, we have identified 14 length variants caused by small additions and deletions (from about 6 to 14 base pairs in length). Three of the 14 length differences are due to mutations at two locations within the D loop, whereas the remaining 11 occur at seven sites that are probably within other noncoding sequences and at junctions between coding sequences. In five of the nine regions of length polymorphism, there is a sequence of five cytosines in a row, this sequence being comparatively rare in coding DNA. Phylogenetic analysis indicates that, in most of the polymorphic regions, a given length mutation has arisen several times independently in different human lineages. The average rate at which length mutations have been arising and surviving in the human species is estimated to be many times higher for noncoding mtDNA than for noncoding nuclear DNA. The mystery of why vertebrate mtDNA is more prone than nuclear DNA to evolve by point mutation is now compounded by the discovery of a similar bias toward rapid evolution by length mutation.

scholarly journals COVID-19 Vaccine Acceptance in Azuay Province, Ecuador: A Cross-Sectional Online Survey

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 678
Author(s):  
Julio Jaramillo-Monge ◽  
Michael Obimpeh ◽  
Bernardo Vega ◽  
David Acurio ◽  
Annelies Boven ◽  
...  
Keyword(s):  
Rural Areas ◽  
Online Survey ◽  
Vaccination Campaign ◽  
Postgraduate Education ◽  
Effective Vaccine ◽  
Cross Sectional ◽  
Lower Education Level ◽  
Vaccination Acceptance ◽  
History Of ◽  
High Level

We investigated the COVID-19 vaccination acceptance level in Azuay province, Ecuador through an online survey from 12th to 26th February (before the start of the COVID-19 vaccination campaign in Ecuador). Overall, 1219 respondents participated in the survey. The mean age was 32 ± 13 years; 693 participants (57%) were female. In total, 1109 (91%) of the participants indicated they were willing to be vaccinated with a COVID-19 vaccine, if the vaccine is at least 95% effective; 835 (68.5%) if it is 90% effective and 493 (40.5%) if it is 70% effective; 676 (55.5%) participants indicated they feared side effects and 237 (19.4%) thought the vaccine was not effective. Older age, having had a postgraduate education, a history of a negative COVID-19 test, a high level of worry of contracting COVID-19, believing that COVID-19 infection can be prevented with a vaccine and understanding there is currently an effective vaccine against COVID-19 were associated with higher vaccination acceptance. A vaccination education campaign will be needed to increase the knowledge of Ecuadorians about the COVID-19 vaccine and to increase their trust in the vaccine. People with a lower education level and living in rural areas may need to be targeted during such a campaign.

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