scholarly journals Identification of Species and Fatty Acid Composition of Microalgae from Raas Beach Sendang Biru Malang and Their Potential as Biodiesel Feedstock

2017 ◽  
Vol 2 (1) ◽  
pp. 51
Author(s):  
Eli Hendrik Sanjaya

Biodiesel is one of the best renewable energy made from triglycerides. Microalgae has a high lipid content include triglycerides, so it can be used as raw material for biodiesel production. Malang has many of beaches which are potent to become sources of microalgae, several beaches are surrounding Sendang Biru. The aims of this research were to identify the species of microalgae from Raas Beach Sendang Biru, determine their yield of lipid and fatty acid composition, and analyse their potential to became feedstock of biodiesel production. This research consists of five stages: identification of microalgae, cultivation, harvesting, determination of the yield of microalgae lipid by soxhlet extraction method, and analysis of the fatty acid composition of lipid by GC-MS. The results showed that the species of microalgae are dominated by Euglena sp and small amount of Gloeocapsa. The lipid yield of dried microalgae is 7.5% and the fatty acids composition are dodecanoic acid (1.6%), tetradecanoic acid (1.3%), hexadecanoic acid (16.6%), 9,12-octadecadienoic acid (25.2%), 9-octadecenoic acid (29.8%), and octadecanoic acid (6.7%). Based on this results, it can be concluded  that the microalgaes from Raas Beach Sendang Biru are potential to become biodiesel feedstock but it still need optimization.

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Ines Ayadi ◽  
Hafedh Belghith ◽  
Ali Gargouri ◽  
Mohamed Guerfali

The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis. On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content. This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose. Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid. An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes. The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material. Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements. Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively. The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran. The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.


2019 ◽  
Vol 24 ◽  
pp. 33-39 ◽  
Author(s):  
R. Ya. Blume ◽  
G. V. Lantukh ◽  
I. V. Levchuk ◽  
S. O. Rakhmetova ◽  
D. B. Rakhmetov ◽  
...  

Aim. Main aim of this research was a comparison of fatty acid composition in seed oil from breeding forms and varieties of hybrid culture tyfon (B. rapa ssp. oleifera f. biennis × (B. rapa ssp. rapifera × B. rapa ssp. pekinensis)) produced in M.M. Gryshko Natl. Botanical Garden of Natl. Academy of Sci. of Ukraine and its parental genotypes: turnip rape and hybrid Holland greens. Methods. Biochemical analysis of oil content as well as chromatographic analysis of fatty acid composition of tyfon were conducted out. Results. Oil fatty acids profiles of tyfon (B. rapa ssp. oleifera f. biennis × (B. rapa ssp. rapifera × B. rapa ssp. pekinensis)) were determined. The highest content of erucic acid was 42.8 % in cultivar Fitopal, the highest content of oleic acid (20.02 %) was in oil of breeding form EOTFVS. Highest content of gondoic acids was indentified in oil of breeding form EOTFV (11.46 %). Conclusions. Taking in account the results of chromatographic analysis the best genotypes for biodiesel production were identified: cultivar Fitopal and breeding form EOTFVS due to their highest content of erucic acid (42.8 % and 41.63 %) and lowest content of linolenic acid (6.99 % and 6.6 %). Keywords: Brassicaceae, hybrid, tyfon, winter vatieties, oil, fatty acids, composition, biodiesel.


1986 ◽  
Vol 32 (10) ◽  
pp. 796-800 ◽  
Author(s):  
S. H. de Boer ◽  
M. Sasser

The cellular fatty acids of Erwinia carotovora were analyzed by gas–liquid chromatography with a fused-silica capillary column. All strains contained, in decreasing order of amount present, 9-hexadecenoic acid (16:1), hexadecanoic acid (16:0), 9-octadecenoic acid (18:1), dodecanoic acid (12:0), 3-hydroxytetradecanoic acid (3-OH-14:0), and tetradecanoic acid (14:0). In addition some strains contained a small amount of 13, 15, 17, and 18 carbon saturated fatty acids. Strains of E. carotovora ssp. atroseptica (Eca) and E. carotovora ssp. carotovora (Ecc) from potato could be differentiated on the basis of three different fatty acid ratios. The Ecc strains had ratios of >3.71 for 12:0/14:0, <4.87 for 16:0/12:0, and <2.70 for 16:1/18:1, while the Eca strains had contrasting values for each of the ratios. Non-potato strains of E. carotovora including strains that were neither Ecc nor Eca on the basis of biochemical reactions were more variable in fatty acid composition than the potato strains, but the fatty acid ratios were generally of the Ecc type.


1977 ◽  
Vol 89 (3) ◽  
pp. 575-582 ◽  
Author(s):  
W. M. F. Leat

SummaryAberdeen Angus and Friesian cattle were reared from 4 months of age to slaughter weight at 18–24 months on either high-barley or high-hay diets. Samples of subcutaneous fat were taken by biopsy at 3 monthly intervals, and the degree of fatness of each animal was estimated ultrasonically prior to slaughter, and by visual inspection of the carcasses.The barley-fed animals gained weight more rapidly, and fattened more quickly than the hay-fed animals with the Angus being fatter than the Friesian at the same age. The percentage stearic acid (C18:0) in subcutaneous fat decreased with age and was replaced by octadecenoic acid (C18:l) and hexadecenoic acid (C16:l), these changes being more rapid in barley-fed than in hay-fed animals. At the same degree of fatness the depot fats of the Friesians were more unsaturated than those of the Angus, and in both breeds the fatter the animal the more unsaturated was its depot fat.In the hay-fed cattle the percentage C16:0 in subcutaneous fat increased during the last half of the experiment and at slaughter the percentage C16:0 was significantly higher, and C18:l significantly lower, in all depot fats compared with those of the barley-fed animals.It is concluded that the fatty acid composition of bovine depot fats is modulated by the degree of fattening, and can be affected by diet.


Author(s):  
Syamsul RAHMAN ◽  
Salengke Salengke ◽  
Abu Bakar TAWALI ◽  
Meta MAHENDRADATTA

Palado (Aglaia sp) is a plant that grows wild in the forest around Mamuju regency of West Sulawesi, Indonesia. This plant is locally known as palado. Palado seeds (Aglaia sp) can be used as a source of vegetable oil because it contains approximately 14.75 % oil, and it has the potential to be used as food ingredients or as raw material for oil production. The purpose of this study was to determine the chemical properties and the composition of fatty acids contained in palado seed oil (Aglaia sp). The employed method involved the use of palado fruit that had been processed to be palado seed and undergoing flouring process. Palado flour was produced by the extraction process by using chloroform solvent with the soxhlet method. The characteristics of the chemical properties in the oil produced were analyzed by using a standard method, including iodine, saponification, and acid values. The analysis of fatty acid composition was conducted by using gas chromatography. The results showed that palado oil extracted with hexane had an iodine value of 15.38 mg/g, saponification value of 190.01 mg KOH/g, and acids value of 1.961 mg KOH/g. The fatty acid composition of the palado seed oil consisted of saturated fatty acids (41.601 %), which included palmitic acid (41.062 %), myristic acid (0.539 %), and unsaturated fatty acids (45.949 %), which included mono-unsaturated fatty acids (MUFA) such as (22.929 %), oleic acid and poly-unsaturated fatty acids (PUFA), which was linoleic acid (23.020 %).


2020 ◽  
Vol 6 (6(75)) ◽  
pp. 13-20
Author(s):  
A.V. Ocheretna ◽  
N.E. Frolova

The fatty acid composition of ostrich fat was studied by chromatographic method. Raw material quality indicators have been experimentally confirmed. Dynamics of growth of acidic number of fat during storage under different conditions is established.


2018 ◽  
Vol 23 ◽  
pp. 24-30 ◽  
Author(s):  
R. Ya. Blume ◽  
G. V. Lantukh ◽  
O. V. Holubets ◽  
S. O. Rakhmetova ◽  
A. I. Yemets ◽  
...  

Aim. Main aim of this research was a comparison of fatty acid composition for seed oils from oil radish breeding forms and varieties produced in Natl. Botanical Garden of Natl. Academy of Sciences of Ukraine. Methods. Biochemical analysis of oil content as well as chromatographic analysis of fatty acid composition of oil radish and its genotypes were conducted out. Results. Oil content in seeds of oil radish (Raphanus var. oleifera sativus L.) was determined. The highest oil content was indicated for variety Kyyanochka – 42 %. Basing on chromatographic analysis of fatty acids of oil radish genotypes most optimal fatty acid composition for biodiesel production was identified in Kyyanochka variety due to high content of short-chained and monounsaturated fatty acids and highest oleic (18:1) acid content – 37.89 %. Conclusions. Taking in account results of chromatographic analysis and agronomic productivity the best genotype for biodiesel production were identified: variety Kyyanochka. Chosen genotype could be used for production of light types of biofuel which have potential to be used as additive for aviation fuel according to the optimal fatty acid composition Keywords: Brassicaceae, oil radish, varieties, breeding forms, oil, fatty acids, biodiesel production.


Sign in / Sign up

Export Citation Format

Share Document