scholarly journals PENGARUH PEMBERIAN MADU TERHADAP STAGING SPERMATOGENESIS DAN SEL LEYDIG PADA MENCIT (Mus musculus) YANG DIINFEKSI Toxoplasma gondii

2020 ◽  
Vol 8 (1) ◽  
pp. 10
Author(s):  
Paraswita Eindah Fitri ◽  
Wurlina Wurlina ◽  
Sri Chusniati ◽  
Lucia Tri Suwanti ◽  
Mufasirin Mufasirin ◽  
...  

The aim of this research is to know about the staging of spermatogenesis and leydig cells in mice that injected by Toxoplasma gondii after giving honey as a preventive substance. This research used 25 mice in age 2-4 month with 20-30 weight. Mice were divided into 5 groups (P0, P1, P2, P3 dan P4). P0 as a control teratment were given aquades in peroral, P1 was a group given honey in doses I (0.08ml/0.2ml/day) during 14 days in peroral, P2 was a group that given infection by T.gondii in doses 103 in peritonial, P3 was given honey in doses I 0.08ml/0.2ml/day) then injected by T.gondii, and P4 was given honey in doses II (0.12ml/0.2ml/day) then injected by T.gondii. This research conducted in 18 days, in day 14th mice were given honey and the day 15th–18th mice injected by T.gondii doses 103. The data result was analyzed with Analysis of Variant (ANOVA) and cotinued with Duncan. Comparision statistical showed with SPSS statistic 20 for Windows. The result of analysis, P0 and P1 were significantly different (p<0.05), with the increase number of spermatogenic and leydig cells. P0 and P2 also significantly different, with the decrease number of spermatogenic and leydig cells. In P2 and P3 group were not significantly different (P>0.05), also in P4 group were not significantly different with P0 and P1. The results of this research showed that honey in doses II can maintain the number of sprematogenic and leydig cells as a preventive substance against T.gondii.

2019 ◽  
Vol 3 (1) ◽  
pp. 33
Author(s):  
Alfina Azkiana ◽  
Boedi Setiawan ◽  
Erma Safitri ◽  
Lucia Tri Suwanti ◽  
Mufasirin Mufasirin ◽  
...  

This research aimed to investigate the effect of folic acid as supportive therapy of spiramycine on weight of foetus to Toxoplasma gondii-infected pregnant mice (Mus musculus). Twenty pregnant female mice were divided into four groups as C -, C +, T1 and T2. C +, T1 and T2 were infected by Toxoplasma gondii. C – and C + administered orally 0.5 ml aquadest, T1 administered orally 130 mg/kg BW spiramycine and 0.052 µg/g BW folic acid and T2 administered orally 0.052 µg/g BW folic acid. Experimental groups received the treatments for 5 days, then animals of each groups were sacrified. Foetuses were dissected out for observation. The weight of fetuses were measured using an analytical balance. The data weight of foetuses was presented descriptively and analyzed by ANOVA test and continued by Tukey HSD. From this study, the weight of foetuses from the pregnant mice of T1 and T2 have difference compared with the controls. The result of this research is folic acid affects the weight of foetuses to Toxoplasma gondii-infected pregnant mice.


Biota ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Mahriani Mahriani ◽  
Susantin Fajariyah ◽  
Eva Tyas Utami

Methoxychlor (MXC) is an insecticide (DDT derivates) that has the potential for bioaccumulation in mammal and causes a disruptive effect on the hepar and reproductive system. This study was done to find out the benefits of curcumin as a natural ingredient to overcome the negative impact of Methoxychlor (MXC) on hepar and male reproductive organ of Balb’C mice (Mus musculus L). The study was carried out in a Completely Randomized Design (CRD) Posttest Only Control Group Design used four treatments and six replications. The curcumin treatment after administration of MXC was carried out by gavage with curcumin doses: 0.05; 0,1; and 0.2 mg/g body weight, every day for two weeks, respectively. Histological observations of the liver, and testis was performed using the paraffin method and Hematoxylin Eosin stained. The results showed that MXC exposure caused liver disruption by increasing the number of pycnotic necrotic hepatocytes and hydrophic degeneration hepatocytes. On the male reproductive organ, MXC caused testis impairment by reducing the number of Sertoli cells and Leydig cells, spermatogenic cell counts, and the diameter of seminiferous tubules. The administration of curcumin at doses of 0.1 mg/g bw in mice exposed to methoxychlor can reduce the number of hydrophic degeneration hepatocytes and tend to reduce the number of pycnotic hepatocytes; and also increase the number of Sertoli cells, the number of spermatogenic cells, and the diameter of the seminiferous tubules, and tend to reduce the amount of Leydig cells. Curcumin treatment tends to recover hepar dan testis disruption of mice that were exposed by MXC.


2021 ◽  
Vol 10 (1) ◽  
pp. 18
Author(s):  
Kukuh Prastyaningtyas ◽  
Rochmah Kurnijasanti ◽  
Rahmi Sugihartuti ◽  
Suherni Susilowati ◽  
Tri Wahyu Suprayogi ◽  
...  

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on the parameters of Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat (40°C). Twenty adult male mice were divided randomly into five groups. The control group (C) mice only received a placebo. Meanwhile, the treatment groups mice were exposed to heat for 45 minutes daily for 36 days and oral administration of placebo, RDFPE of 250, 500, and 1000mg/kg BW for T0, T1, T2, and T3, respectively. The result showed that heat exposure on mice (T0 group) caused a lower of all of the parameters (p <0.05) than normal mice (control group, C). RDFPE administration at a dose of 250 mg/kg BW (T1 group) and 500 mg/kg BW (T2 group) resulted in a higher value of those parameters (p <0.05) compared to the T0 group. All those parameters of the T2 group (dose of 500 mg/kg BW) were not significantly different (p >0.05) than the control group (normal mice). However, the higher dose of RDFPE (1000 mg/kg BW, T3 group) resulted in the lower values of those parameters (p <0.05) than those of the T2 group. It could be concluded that 500mg/kg BW dose of RDFPE could return Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat.


2019 ◽  
Author(s):  
◽  
Bruno Fitte

El objetivo de este trabajo fue integrar conocimientos parasitológicos y socio-ambientales a través del estudio de parásitos y patógenos de roedores urbanos presentes en el Gran La Plata, enfatizando en el estudio de especies de importancia sanitaria humana y animal. Para esto, se identificaron a las especies parásitas y patógenas presentes en las tres especies de roedores urbanos (Rattus norvegicus, Rattus rattus y Mus musculus), capturados en siete barrios con diferente grado de urbanización y con distintas características ambientales y estructurales. Se analizaron las relaciones entre la prevalencia / abundancia media de los taxones parásitos y patógenos con variables de los hospedadores y del ambiente. Por otro lado, se evaluó la percepción social con respecto a roedores urbanos y patologías asociadas en dos barrios con características estructurales y ambientales contrastantes, y resultados parasitológicos distantes. Paralelamente, en uno de esos barrios, el cual reveló una de las mayores riquezas de parásitos y patógenos, se propuso un programa modelo que consistió en una serie de talleres y actividades con alumnas/os de dos escuelas secundarias, con el fin de proponer acciones que tiendan a mitigar la problemática a través del intercambio de saberes y la generación de estrategias en conjunto para el control y prevención a nivel barrial, considerando las observaciones de la población y los resultados parasitológicos obtenidos. Se analizaron un total de 136 individuos roedores a partir de un esfuerzo de muestreo de 1046 trampas/noche de trampas tipo Sherman; 962 trampas/noche de jaulas; y 788 trampas/noche de trampas de golpe. Se identificaron un total de 16 taxones de parásitos y patógenos, correspondiendo a protozoos (n=3), helmintos (n=10), virus (n=1), bacterias (n=2). Del total de patógenos y parásitos hallados, siete fueron identificados como zoonóticos: Toxoplasma gondii (protozoos); Hymenolepis diminuta, Rodentolepis nana y Strobilocercus fasciolaris (helmintos); Hantavirus Seoul (virus); Leptospira borgpetersenii y Leptospira interrogans (bacterias). A su vez, se registró el protozoo enzoótico Neospora caninum. Se aportaron detalles morfológicos y biométricos en las especies de helmintos. Hantavirus Seoul en el hospedador R. rattus representa el primer registro en Argentina. Además, T. gondii y el protozoo enzoótico N. caninum corresponden a los primeros registros en roedores en Argentina, Todos los demás hallazgos de parásitos y patógenos (incluidos los no zoonóticos), corresponden a nuevos registros para el área del Gran La Plata. Se evaluaron diferentes factores del hospedador y del ambiente para explicar la composición y abundancia del ensamble parasitario en cada individuo hospedador, resultando la especie hospedadora el único estadísticamente significativo. Así, se comprobó que los ensambles de parásitos y patógenos son similares entre las especies de Rattus spp., pero que difieren del observado en M. musculus. Para cada especie hospedadora se analizaron en forma separada las prevalencias y abundancias de cada taxón parásito resultando estadísticamente significativa las siguientes asociaciones: la prevalencia y abundancia de S. fasciolaris y la prevalencia de N. brasiliensis en R. norvegicus fueron mayores en la estación fría; mientras que la presencia de arroyo en el barrio explicó la prevalencia de N. brasiliensis en R. rattus y S. ratti en R. norvegicus. Por otro lado, el estudio sobre percepción social reflejó una preocupación común en relación al rol que juegan los roedores urbanos como reservorios y trasmisores de enfermedades zoonóticas. Las patologías mencionadas con más frecuencia por la población estudiada fueron el Hantavirus, la rabia, y la Leptospirosis. Además, a partir de las respuestas obtenidas se pudo concluir en que en los barrios periféricos la presencia de roedores urbanos dentro de los hogares y en el barrio en general es mayor que en el Casco urbano de La Plata. También, a partir del programa planteado en las escuelas secundarias, se comprobó que las/os alumnos reconocían la existencia de roedores en el barrio y hogares, pero un alto porcentaje no conocía acerca de su implicancia sanitaria. Esto cambió hacia el final del programa, cuando las/os alumnas/os fueron capaces de identificar diferentes patologías asociadas a los roedores, y plantear alternativas y medidas de posible aplicación a nivel barrial para el control de roedores urbanos. Así, se resalta la importancia de focalizar esfuerzos que permitan promover acciones comunitarias, principalmente en estas áreas marginales presentes en toda Latinoamérica, las cuales en su mayoría no están nunca incluidas en proyectos de urbanización formal.


Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 27 ◽  
Author(s):  
Vladimir Ivovic ◽  
Sandra Potusek ◽  
Elena Buzan

To assess the prevalence of Toxoplasma gondii infection in native and commensal rodents as indicators of environmental pollution, we analyzed brain tissue from small mammals collected on legal and illegal waste sites in the Slovenian and Croatian parts of Istria. A total of 136 animals and five species of the family Muridae were analyzed: black rat (Rattus rattus), domestic mouse (Mus musculus), wood mouse (Apodemus sylvaticus), striped field mouse (Apodemus agrarius), and yellow-necked mouse (Apodemus flavicollis). Using quantitative Polymerase Chain Reaction (qPCR), T. gondii DNA was detected in four homogenized brain tissue samples (2.94%), from all of the analyzed species, except black rat. Out of these, two samples, domestic mouse (Mus musculus) and wood mouse (Apodemus sylvaticus) had sufficient DNA for genotyping of T. gondii isolates in which we demonstrated the presence of clonal type II using RFLP PCR with four markers (SAG1, SAG2, GRA6 and GRA7). Three of four infected animals (75%) were collected on dumpsites.


Parasitology ◽  
1998 ◽  
Vol 116 (4) ◽  
pp. 299-304 ◽  
Author(s):  
M. R. OWEN ◽  
A. J. TREES

Captive-bred Mus musculus (house mice) and Apodemus sylvaticus (field mice) were each infected with 50 oocysts of Toxoplasma gondii M1 strain per os and infection in them and their offspring was assessed by polymerase chain reaction (PCR) amplification of the T. gondii B1 gene in brain tissue and by serology, using the modified agglutination test (MAT). The chronically infected female A. sylvaticus (n=10) and M. musculus (n=23) were mated at least 6 weeks after infection (and subsequently to produce up to 6 litters) and their pups examined 3 weeks after weaning at 6 weeks of age. By PCR, in offspring of A. sylvaticus and M. musculus respectively, vertical transmission was demonstrated in 82·7% (n=83) and 85·0% (n=207) of all pups (N.S., P>0·05), 95% (n=21) and 100% (n=30) of all litters (N.S., P>0·05), with a mean (±S.E.) proportion of each litter infected of 0·87 (0·06) and 0·86 (0·04) (N.S., P>0·05). There was no change in any of these variables between first and subsequent litters. By serology, whilst MAT suggested 100% vertical transmission in A. sylvaticus, it under-estimated rates of infection in offspring of M. musculus. A limited series of bioassays from M. musculus tissues confirmed the good correlation of PCR and the poor correlation of MAT with mouse inoculation. These results indicate that vertical transmission in A. sylvaticus and M. musculus is extremely efficient and probably endures for the life of the breeding female. This mechanism favours parasite transmission and dispersion by providing a potential reservoir of infection in hosts predated by the cat.


Acta Tropica ◽  
2021 ◽  
pp. 105938
Author(s):  
Larissa Staurengo-Ferrari ◽  
Raquel Arruda da Silva Sanfelice ◽  
Julia Bagatim de Souza ◽  
Joao Paulo Assolini ◽  
Dayane Priscila dos Santos ◽  
...  

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