Rambusa (Passiflora foetida L) vs. Free Radicals:  In Vitro Study with DPPH Method

ABSTRAK Rambusa (Passiflora foetida L) adalah tumbuhan obat yang banyak terdapat di berbagai daerah di Indonesia, termasuk di Kalimantan Tengah. Beberapa bagian tumbuhan dari rambusa diketahui memiliki aktivitas antioksidan termasuk di bagian daunnya. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari daun rambusa yang berasal dari Kalimantan Tengah. Metode yang digunakan adalah metode 2,2-diphenyl-1-picrylhydrazyl (DPPH) yang diukur serapannya dengan Spektrofotometer UV Vis. Hasil yang diperoleh menunjukkan nilai IC50 dari ekstrak etanol daun rambusa senilai 93,269 μg/mL. Meskipun nilai IC50 yang diperoleh lebih rendah dibandingkan senyawa antioksidan standar seperti kuersetin, nilai IC50 dari ekstrak etanol daun rambusa asal Kalimantan Tengah masih lebih tinggi dibandingkan daun rambusa yang diperoleh dari daerah lain Kata Kunci—antioksidan, DPPH, Kalimantan Tengah, Passiflora foetida, rambusa ABSTRACT Rambusa (Passiflora foetida L) is a medicinal plant that is widely found in various regions in Indonesia, including in Central Kalimantan. Some parts of the plant of rambusa are known to have antioxidant activities including in the leaves. This study aims to determine the antioxidant activity of rambusa leaves from Central Kalimantan. The method used was 2,2-diphenyl-1-picrylhydrazyl (DPPH) method which was measured by UV Vis spectrophotometer. The results obtained showed IC50 values of ethanol extract of rambusa leaves worth 93.269 μg/mL. Although the IC50 values obtained were lower than the standard antioxidant compounds such as quercetin, the IC50 value of the ethanol extract of the leaves of rambusa from Central Kalimantan was still higher than that of the rambusa leaves obtained from other regions or some other medicinal plant extracts. Keywords— antioxidants, DPPH, Central Kalimantan, Passiflora foetida, rambusa

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In-Vitro Study of Antioxidant Activities from Ethanol Extracts of Akar Kuning (Arcangelisia flava)

Jurnal Surya Medika ◽

10.33084/jsm.v4i2.594 ◽

2019 ◽

Vol 4 (2) ◽

pp. 66-71

Author(s):

Suratno Suratno ◽

Muhammad Ikhwan Rizki ◽

Mohammad Rizki Fadhil Pratama

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

In Vitro Study ◽

Ethanol Extract ◽

Antioxidant Compounds ◽

Dpph Method ◽

Ic50 Values ◽

Stem Extract ◽

Ethanol Extracts

This study aims to determine the antioxidant activity of akar kuning (Arcangelisia flava) stem extract from Central Kalimantan using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. This research includes extraction of simplicia and test of antioxidant activity by DPPH method. Based on the analysis of antioxidant activity on ethanol extract from yellow root plant stem IC50 values of 136.81 ppm were obtained. This IC50 value indicates the antioxidant strength of the yellow root plant stems including in the moderate category. Further research on antioxidant activity in the fraction of akar kuning stems needs to be done to determine the composition of the antioxidant compounds in each solvent.

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UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽

10.35799/pha.9.2020.30031 ◽

2020 ◽

Vol 9 (3) ◽

pp. 451

Author(s):

Rizkah V. Mokoginta ◽

Herny E. I. Simbala ◽

Karlah L.R Mansauda

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Ethanol Extract ◽

Antioxidant Compounds ◽

Onion Plant ◽

Dpph Method ◽

Ic50 Values ◽

Experimental Laboratory ◽

Ethanol Extracts ◽

Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

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Functional Effects of Pomegranate Peel Extracts on Milk: Antibacterial Measurements, Antioxidant Activities, and Photochemical Characterizations

Journal of Biobased Materials and Bioenergy ◽

10.1166/jbmb.2021.2097 ◽

2021 ◽

Vol 15 (5) ◽

pp. 571-579

Author(s):

Rokayya Sami ◽

Ebtihal Khojah ◽

Mona Alharbi ◽

Amina A. M. Al-Mushhin ◽

Mohammed Saeed Alkaltham ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging ◽

Water Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Pomegranate Peel ◽

Bacteria Growth ◽

Wonderful Variety ◽

Ic50 Values

Consumers are interested in antimicrobial by-products from natural sources. The present study aims to estimate the antibacterial effects of different concentrations and solvent extracts such as methanol, ethanol, and water extract of pomegranate peels with the milk quality application in-vitro. Listeria monocytogens, E. coli, Salmonella, and Staphylococcus aureus were used as bacteria growth indicators in two pomegranate varieties (i.e., Wonderful and Francis). Besides, evaluating somephysical characterization, antibacterial and antioxidant activities. Results showed that the highest inhibition zone of 18.88 mm was observed with 90% ethanol extract of Wonderful variety against Listeria monocytogens, at 0, 12.5, 25, and 50 mg/mL concentrations, while the lowest inhibition zone of 11.29 mm was observed with 90% methanol extract of Francis variety, at 12.5 mg/mL concentration. The milk treated with WPE of Wonderful variety maintained the bacterial growth from 1.19 ±0.29 x 109 to 1.43 x 1011 CFU/mL at 4 °C. Punicalagin was the most abundant polyphenolic compound (165.05–190.43 µg/mL) followed by gallic acid (153.08–177.65 µg/mL), and p-Hydroxybenzoic acid (91.29–92.5 mg/mL). Acetaldehyde (23.09–27.15 PPM), followed by acetoin (9.65–15.33 PPM) were the most predominant volatile compounds. The WPE treated milk maintained the sensory evaluations such as taste, color, texture, and overall acceptance longer time longer time (i.e., 144 h) than FPE treated milk (i.e., 168 h) at 4°C. DPPH and ABTS radical scavenging of capacity (IC50 values) of FPE treated milk were found to be lower than WPE treated milk (i.e., 159.65 µg/mL and 131.87 µg/mL), respectively. While, FPE treated milk reported a higher viscosity content than WPE treated milk (13.11 cP). In conclusion, pomegranate peel extracts especially Wonderful variety may be used to prolong the shelf-life of milk in dairy products manufacturers.

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Secondary Metabolites from the Barks of Eucommia ulmoides and Their Bioactivities

Journal of the chemical society of pakistan ◽

10.52568/000708/jcsp/41.01.2019 ◽

2019 ◽

Vol 41 (1) ◽

pp. 169-169

Author(s):

Wen Yan Liu Wen Yan Liu ◽

Zi Ling Zi Ling ◽

Dong Dong ◽

Ke Huan Li Ke Huan Li ◽

Jian Hua Shao Jian Hua Shao ◽

...

Keyword(s):

Secondary Metabolites ◽

Antioxidant Activities ◽

Ethanol Extract ◽

2D Nmr ◽

Eucommia Ulmoides ◽

Antioxidant Power ◽

Ic50 Values ◽

Ferric Reducing Antioxidant Power ◽

Intense Activity

Eucommia ulmoides Oliv., exhibiting diverse bioactivities including antimicrobial, antioxidant, neuroprotective, anti-tumor and anti-inflammatory properties, has long been known as an active ingredient commonly used in antihypertensive herbal prescriptions in China. The 95% ethanol extract of the barks of E. ulmoides showed antimicrobial and antioxidant activities and then was isolated by bioactivity-guided fractionation to obtain a new cinnamyl glucoside, 1-O-trans-cinnamoyl-β-D-apiofuranosyl-(1→6)-β-D-glucopyranose (1), together with twenty known secondary metabolites (2–21). And their structures were identified by extensive spectroscopic analyses, including 1D and 2D NMR, UV, IR and HRESIMS, and chemical methods. All secondary metabolites were evaluated for their antimicrobial and antioxidant activities in vitro. Compounds 7, 8, 11, and 21 exhibited moderately antimicrobial activity. In the antioxidant activity assay, compounds 3, 7, 11, and 14 showed intense activity in ferric reducing antioxidant power (FRAP) with the values of 9.82 mmol/g, 19.25 mmol/g, 14.52 mmol/g, and 12.58 mmol/g in comparison to Trolox (9.02 mmol/g). And compounds 2, 11, and 14–16 showed more potent activity with IC50 values ranging from 18.34 to 24.36 M than Trolox (26.35 M) in ABTS+• scavenging assay.

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Phytochemical composition and antioxidant activity of Malus baccata (L.) Borkh. fruits

Food Science and Applied Biotechnology ◽

10.30721/fsab2020.v3.i1.46 ◽

2020 ◽

Vol 3 (1) ◽

pp. 47

Author(s):

Nadezhda Petkova ◽

Manol Ognyanov ◽

Blaga Inyutin ◽

Petar Zhelev ◽

Panteley Denev

Keyword(s):

Phenolic Compounds ◽

Antioxidant Activities ◽

Ethanol Extract ◽

Titratable Acidity ◽

Total Phenolic ◽

Total Phenolic Compounds ◽

Frap Assay ◽

Phytochemical Composition ◽

Malus Baccata

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

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FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.90 ◽

2018 ◽

Vol 10 (1) ◽

pp. 407

Author(s):

Dewi Kumala Putri ◽

Berna Elya ◽

Nuraini Puspitasari

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Hexane Extract ◽

Phytochemical Screening ◽

Active Fraction ◽

Leaf Extracts ◽

Antioxidant Power ◽

Dpph Method ◽

Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

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Evaluation of antioxidant activities of ethanol extract from Ligusticum subjected to in-vitro gastrointestinal digestion

Food and Chemical Toxicology ◽

10.1016/j.fct.2017.12.035 ◽

2018 ◽

Vol 119 ◽

pp. 417-424 ◽

Cited By ~ 5

Author(s):

Huifang Ge ◽

Yazhen Chen ◽

Jicheng Chen ◽

Jingjing Tian ◽

Xiaofeng Liang ◽

...

Keyword(s):

Antioxidant Activities ◽

Ethanol Extract ◽

Gastrointestinal Digestion ◽

In Vitro Gastrointestinal Digestion

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Biological activity from indigenous medicinal plants of Mauritius

Pure and Applied Chemistry ◽

10.1351/pac200577010041 ◽

2005 ◽

Vol 77 (1) ◽

pp. 41-51 ◽

Cited By ~ 19

Author(s):

A. Gurib-Fakim ◽

H. Subratty ◽

F. Narod ◽

J. Govinden-Soulange ◽

F. Mahomoodally

Keyword(s):

Medicinal Plant ◽

Plant Extracts ◽

Biological Properties ◽

Pharmacological Properties ◽

Plant Materials ◽

In Vivo Screening ◽

Medicinal Plant Extracts ◽

Minor Ailments

The Mauritian population has a long tradition in the use of ethno-medicine, and the practice is still strong, especially in the treatment of minor ailments. Such interest stems from an existing culture, and many “tisanes” are still prepared from plant materials and sold in several markets around the island.This paper will focus on the various chemical/biological screening techniques currently being used to evaluate the biological properties of medicinal plant extracts. Particular emphasis will be put on extraction and various screening for biological/pharmacological properties. Due consideration will be given to the pharmacological approaches that utilize different animal models for the in vitro and in vivo screening of medicinal plant extracts.

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