scholarly journals Stability studies of mefenamic acid Self-Nanoemulsifying Drug Delivery System (SNEEDS) preparation with oleic acid as the oil phase

2020 ◽  
Vol 16 (2) ◽  
pp. 130-143
Author(s):  
Yandi Syukri ◽  
Septiani Eka Cahyani ◽  
Bambang Hernawan Nugroho
Keyword(s):  
Oleic Acid ◽  
Mefenamic Acid ◽  
Tween 80 ◽  
Stability Studies ◽  
Storage Test ◽  
Peg 400 ◽  
Accelerated Storage ◽  
The Stability ◽  
Accelerated Storage Test

Background: Mefenamic acid is a non-steroidal anti-inflammatory drug (NSAID) with low solubility in water. Self-Nanoemulsifying Drug Delivery Systems (SNEDDS) play a role to improve the solubility and bioavailability of mefenamic acid. Objective: This study aimed to determine the stability of mefenamic acid in SNEDDS formulation through various stability studies. Methods: The stability studies conducted consisted of centrifugation test, heating-cooling cycle test, freezethaw cycle test, robustness to dilution, accelerated storage test, and determination of drug content. Results: The centrifugation test, heating-cooling cycle test, and freeze-thaw cycle test showed no phase separation in the samples. The robustness to dilution and accelerated storage test resulted in 2 formulas of mefenamic acid loaded SNEDDS having good stability with 10% oleic acid, 80% tween 80, 10% PEG 400 and 10% oleic acid, 70% tween 80, 20% PEG 400. The determination of drug content in both of these formulations showed 98.20 ± 0.04% and 90.98 ± 0.06%. Conclusion: The SNEDDS formulation of mefenamic acid in this study had good stability. Keywords: SNEDDS, mefenamic acid, stability study, oleic acid

Solubility Studies and Validation of Lovastatin using High Performance Liquid Chromatography Method

2021 ◽  
pp. 6285-6288
Author(s):  
Nurhabibah Nurhabibah ◽  
A.K. Nugroho ◽  
Ronny Martien ◽  
Endang Lukitaningsih
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Oleic Acid ◽  
High Performance ◽  
Tween 80 ◽  
Tween 20 ◽  
Chromatography Method ◽  
Peg 400 ◽  
Solubility Studies ◽  
Liquid Chromatography Method

This study aimed to determine the solubility of lovastatin (LV) in different oil, surfactant, and co-surfactant using the high-performance liquid chromatography method. LV was solubility studies in different vehicle. The different vehicle used almond oil, sunflower oil, oleic acid, olive oil, soybean oil, and corn oil, isoprophyl myristate, myoglyol, tween 80, tween 20, and cremophor R.H. 40, propylene glycol, and PEG 400. Each of them was added lovastatin until saturated. The mixtures were mixing, sonicating, putting in the water bath and standing for 24 hours, then centrifugated. Each of the aliquot 2 µL diluted with acetonitrile and determination of concentration lovastatin using HPLC, with detector ultraviolet at 237 nm. Before determinate LV validated, and curve calibration at range 2-16 µg/mL was made. This study using the HPLC method with detector UV 237 nm, Agilent C 18 (4.6 x 150 mm 5 µ) column, and acetonitrile: water (70:30 v/v) as mobile phase. Calibration curve of lovastatin at the range 2-16 µg/mL with linear regression 0.999. Accuracy and precision showed that. Lovastatin has high soluble in oleic acid, tween 80, and PEG 400.

FACTORS AFFECTING THE STORAGE OF DEHYDRATED PORK

1945 ◽  
Vol 23f (1) ◽  
pp. 9-21
Author(s):  
Jesse A. Pearce
Keyword(s):  
Storage Temperature ◽  
Storage Test ◽  
Factors Affecting ◽  
Accelerated Storage ◽  
Relative Value ◽  
Keeping Quality ◽  
P 16 ◽  
One Year ◽  
Accelerated Storage Test

In an accelerated storage test at 60 °C. fat levels of 20 and 30% were observed to have no effect on keeping quality of dehydrated pork. Measurements on dried product stored in paper-bodied containers for one year at temperatures from −17.8 °C. to 36.7 °C. showed 15.6 °C. to be less desirable than 23.9°, 0°, or −17.8 °C. Dehydrated pork was stored in tin-plate containers for periods of one year at 23.9 °C. and 36.7 °C. with little decrease in palatability. No difference in storage life was demonstrated between cured and uncured pork, or, as a result of differences in drying times, moisture content |or storage temperature. The product prepared on an atmospheric double-drum drier deteriorated a little more rapidly than vacuum–tray- or tunnel–tray-dried material. The effectiveness of container materials, tin-plate, Reynolds' metal A-10, Dewey and Almy P-16, and 300 MST cellophane, was evaluated and their relative value for dehydrated pork fell in that order.

Application of accelerated storage test to lyophilized plant viruses

1996 ◽  
Vol 10 (12) ◽  
Author(s):  
Angela Yordanova ◽  
Elisaveta Stoimenova ◽  
Todor Donev
Keyword(s):  
Plant Viruses ◽  
Storage Test ◽  
Accelerated Storage ◽  
Accelerated Storage Test

scholarly journals VIRULENCE AND MORPHOLOGICAL CHARACTERISTICS OF MAMMALIAN TUBERCLE BACILLI

1947 ◽  
Vol 86 (2) ◽  
pp. 175-184 ◽  
Author(s):  
Gardner Middlebrook ◽  
René J. Dubos ◽  
Cynthia Pierce
Keyword(s):  
Oleic Acid ◽  
Morphological Characteristics ◽  
Tween 80 ◽  
Water Dispersible ◽  
Varying Thickness ◽  
Solid Media ◽  
Solid Agar ◽  
Surface Active ◽  
Morphological Differences

Experimental infection of the mouse can be used for the determination of virulence of cultures of mammalian tubercle bacilli. The relative virulence of such cultures for the mouse is approximately the same as for the guinea pig. Cultures of virulent and avirulent variants of mammalian tubercle bacilli grown in the depth of Tween 80-albumin liquid medium, on the surface of solid agar modifications of this medium, and on the surface of a liquid modification of this medium exhibit consistent morphological differences. All virulent cultures tend to form microscopically demonstrable serpentine cords of varying thickness and length consisting of highly acid-fast bacilli oriented in parallel along the long axis of the cord. The formation of cords appears to be an important factor in conditioning the ability of cultures to spread on the surface of liquid and solid media. It can be inhibited by the addition to the medium of the surface-active water-dispersible oleic acid ester, Tween 80. Avirulent variant bacilli grow in a more or less non-oriented fashion. They have never been observed to form cords under any condition of growth and are much less acid-fast than the virulent cultures when grown in Tween-albumin medium. Two strains of mammalian tubercle bacilli which are intermediate in degree of virulence between the fully virulent and the avirulent variants also exhibit intermediate morphological characteristics.

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